Polyethyleneimine capped silver nanoclusters based turn-off-on fluorescence sensor for the determination of glutathione.

A polyethyleneimine capped silver nanoclusters (PEI-AgNCs) based turn-off-on fluorescence sensor has been developed to determine glutathione (GSH) effectively. The fluorescence intensity of silver nanoclusters (AgNCs) has been quenched by Cu(II) and recovered by adding GSH. The quenching of fluorescence intensity of PEI-AgNCs by Cu(II) and recovery of the emission intensity of PEI-AgNCs after the addition of GSH is supposed to be ground state adduct formation. Due to the greater affinity of Cu(II) towards GSH compared to that to PEI-AgNCs, the defragmentation of PEI-AgNCs-Cu(II) adduct occurs after the addition of GSH to the solution, resulting in the recovery of emission intensity of PEI-AgNCs. Characterisation studies of the probe have been done using FT-IR spectroscopy, XPS analysis, XRD analysis, UV-visible and Fluorescence spectrophotometry, EDX spectroscopy and TEM analysis. Different experimental parameters were optimised. Under optimised analytical conditions, the sensor showed a wide linear range for the quantification of GSH from 1.00 × 10-4 M to 3.00 × 10-6 M with a detection limit (LOD) of 8.00 × 10-7 M. Selectivity and interference studies were done in the presence of different structurally similar and coexisting species of GSH in blood. The practical utility of the proposed sensor has been validated in artificial blood serum.

Lysozyme functionalized silver nanoclusters as a dual channel optical sensor for the effective determination of glutathione.

This article describes the development of a facile and efficient fluorescence sensor for the determination of glutathione (GSH). Presence of the antioxidant glutathione in blood serum is considered as a biomarker for catastrophe like colorectal cancer. Silver nanoclusters with strong fluorescence and good water solubility synthesized from relatively cheaper precursors are one of the species very much explored in fluorescence sensors and bioimaging. Here, Chicken egg derived-lysozyme functionalized silver nanoclusters (Lyz AgNCs) with red fluorescence emission has been synthesized and developed to a turn-off fluorescence sensor for GSH through which colorimetric determination is also possible. Due to the ground state 'Ag-S' interaction between Lyz AgNCs and GSH, the determination of the analyte is possible from 1.00 × 10-5 M to 1.00 × 10-6 M via fluorimetric and from 9.00 × 10-6 to 8.00 × 10-7 M via spectrophotometric techniques with a limit of detection 2.86 × 10-7 M and 4.76 × 10-7 M, respectively. Selectivity of the sensor has been studied and applicability of the sensor in artificial blood serum samples has been demonstrated.

Minimum data and core outcomes for subacute rehabilitation: A scoping review.

OBJECTIVE: In clinical practice and research, standardised sets of data and outcomes are routinely collected to facilitate data comparison, benchmarking and quality improvement. Most existing data sets are condition-specific and cannot be applied to all patients in a given clinical setting. This review aimed to determine whether the development of a minimum data set for subacute rehabilitation is feasible by collating and comparing existing rehabilitation minimum data sets and core outcome sets. DATA SOURCES: Published literature was identified through database searches (Scopus, PubMed, EMBASE, CINAHL and the COMET Initiative) in September 2021. Additional data sets were identified through a grey literature search. REVIEW METHODS: This review was conducted in alignment with the PRISMA-ScR recommendations. Datasets were included if they were published in English, designed for adults, and intended for use in subacute rehabilitation. Data were extracted and taxonomically organised to identify commonalities. Items present in ≥50% of data sets were considered common. RESULTS: Twenty minimum data sets and seven core outcome sets were included. There were 29 common minimum data set domains, with 19 relating to Patient Information, seven relating to Outcomes, two relating to Service Delivery and one relating to Provider Demographics. Four common domains were identified within the Core Outcome Set analysis, which all related to Life Impact, specifically Physical Functioning (86%), Emotional Functioning/Wellbeing (57%), Social Functioning (86%) and Global Quality of Life (100%). CONCLUSION: Common item domains in conditions requiring subacute rehabilitation have been identified, suggesting that development of a dataset for subacute rehabilitation may be feasible.

Sustaining allied health telehealth services beyond the rapid response to COVID-19: Learning from patient and staff experiences at a large quaternary hospital.

The patient, clinician and administration staff perspectives of telehealth (specifically videoconferencing) services provided by Allied Health Professions (AHP) at a large quaternary hospital were explored. The purpose was to understand stakeholders' perceptions of the service during initial COVID-19 restrictions and examine factors that influenced the implementation and sustained use of telehealth. A sequential mixed-methods approach was undertaken. Stage 1 involved surveys completed by patients (n = 109) and clinicians (n = 66) who received and provided care via telehealth, respectively, across six AHP departments. Stage 2 involved focus groups with clinicians (n = 24) and administrative staff (n = 13) to further examine implementation and sustainability factors.All participant groups confirmed that telehealth was a valid service model and valued the benefits it afforded, particularly during COVID-19 restrictions. Both patients and clinicians reported that not all AHP services could be delivered via telehealth and preferred a blended model of telehealth and in-person care. Increased administrative staff assistance was needed to support growing telehealth demand. Main factors to address are the need to expand AHP telehealth models and workforce/patient training, improve workflow processes and enhance technical support.Despite rapid implementation, telehealth experiences were overall positive. Study findings are being used to generate solutions to enhance and sustain AHP telehealth services.

Tannic Acid Capped Copper Nanoclusters as a Cost-Effective Fluorescence Probe for Hemoglobin Determination.

For the first time, we report on a copper nanoclusters based fluorescence sensor for hemoglobin (Hgb). The aggregation-induced quenching of tannic acid capped copper nanoclusters' (TACuNCs) fluorescence by a Hgb-H2O2 mixture that mimics the Fenton's reagent is used here for the selective determination of Hgb. It is possible to effectively determine Hgb using this sensitive and cost-effective sensor in the linear range of 5.0 × 10-8 to 4.0 × 10-9 M with a detection limit of 5.6 × 10-10 M. The practical utility of the sensor is evident from the good recovery values obtained from Hgb spiked with artificial blood serum.

Allied health primary contact services: results of a 2-year follow-up study of clinical effectiveness, safety, wait times and impact on medical specialist out-patient waitlists.

Objective Long specialist out-patient waitlists are common in public health facilities, but not all patients require consultation with a medical specialist. Studies of single allied health primary contact services have shown they provide timely, appropriate care and reduce demand on medical specialist out-patient waitlists. This study evaluated the collective benefits across multiple allied health primary contact services and models to determine their clinical effectiveness, safety, timeliness of care and impact on medical specialist out-patient waitlists. Method Using a prospective observational study design, data were collected and analysed for patients attending 47 allied health primary contact services in Queensland public hospitals over a 2-year period. Outcomes reported are global status, adverse events, wait times and impact on medical specialist out-patient waitlists. Results In all, 10634 patients were managed in and discharged from the allied health services. Most adult patients (80%) who attended at least two consultations reported an improvement in health status. No adverse events were attributed to the model of care. Approximately 68%, 44% and 90% of urgent, semi-urgent and non-urgent out-patients respectively were seen within clinically recommended time frames. Between 35% and 89% of patients were removed from out-patient waitlists without medical specialist consultation across the service models. Conclusions Allied health primary contact services provide safe, effective and timely care. The impact on medical specialist out-patient waitlists varied depending on service model and pathway characteristics. What is known about this topic? Most studies of allied health primary contact services have focused on the management of patients on orthopaedic specialist out-patient waitlists by a physiotherapist. These studies of either individual services or groups of services with the same model cite benefits, including reduced waiting times, high levels of patient and referrer satisfaction, improved conversion to surgery, cost-effectiveness and more effective utilisation of medical specialists. What does this paper add? This paper highlights that, collectively, allied health primary contact services are safe, effective and provide timely care. The proportion of patients independently managed and removed from various medical specialist out-patient waitlists and the services involved are reported, demonstrating the variety of service models. This study reports outcomes for primary contact services for which there is a dearth of published literature, including dietician services for patients on gastroenterology waitlists, speech pathology and audiology services for patients on ear, nose and throat waitlists, occupational therapy hand services for patients on orthopaedic waitlists and physiotherapy led pelvic-health services for patients on gynaecology waitlists. Possibilities for efficiency gains are identified and discussed. What are the implications for practitioners? Health service managers should consider allied health primary contact services as a viable option to increase specialist out-patient capacity. Service model characteristics that maximise impact on medical specialist out-patient waitlist management are highlighted to inform resource allocation.

Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases.

The rise of emerging infectious diseases (EIDs) as well as the increase in spread of existing infections is threatening global economies and human lives, with several countries still fighting repeated onslaught of a few of these epidemics. The catastrophic impact a pandemic has on humans and economy should serve as a reminder to be better prepared to the advent of known and unknown pathogens in the future. The goal of having a set of initiatives and procedures to tackle them is the need of the hour. Rapid detection and point-of-care (POC) analysis of pathogens causing these diseases is not only a problem entailing the scientific community but also raises challenges in tailoring appropriate treatment strategies to the healthcare sector. Among the various methods used to detect pathogens, Electrochemical Biosensor Technology is at the forefront in the development of POC devices. Electrochemical Biosensors stand in good stead due to their rapid response, high sensitivity and selectivity and ease of miniaturization to name a few advantages. This review explores the innovations in electrochemical biosensing based on the various electroanalytical techniques including voltammetry, impedance, amperometry and potentiometry and discusses their potential in diagnosis of emerging and re-emerging infectious diseases (Re-EIDs), which are potential pandemic threats.

A uniform data set for determining outcomes in allied health primary contact services in Australia.

The project aim was to develop and implement a set of metrics to capture and demonstrate the performance of newly established allied health primary contact services. Selection of the metrics and performance indicators was guided by an existing state-wide data collection system and from a review of the published literature. The metrics were refined after consultation with a working group of health service managers and clinicians. The data collection and reporting framework were developed for use in allied health primary contact services and implemented at public health facilities in Queensland, Australia. The set of metrics consists of 18 process and outcome measures. Patient-reported metrics include the global rating of change scale and patient satisfaction. Service metrics include wait times; referral source; triage category; diagnosis; occasions of service; referrals and investigations initiated; effects; care duration; discharge status; waitlist reinstatement reasons; treatment non-completion reasons; and expedited care. Safety, patient demographics and service improvement metrics were included. The metrics will enable analysis of the effectiveness of allied health primary contact services and will facilitate reporting, advocacy, service improvement, service continuity and research. The metrics are suitable for use by all providers of allied health primary contact services in hospital and primary care settings.

Process to establish 11 primary contact allied health pathways in a public health service.

Objective Faced with longstanding and increasing demand for specialist out-patient appointments that was unable to be met through usual medical consultant led care, Metro North Hospital and Health Service in 2014-15 established 11 allied health primary contact out-patient models of care. Methods The models involved six different allied health professions and nine specialist out-patient departments. Results All the allied health models have been endorsed for continuation following demonstration of their contribution to managing demand on specialist out-patient services. Conclusion This paper describes key features of the allied health primary contact models of care and presents preliminary data including new case throughput, effect on wait times and enablers and challenges for clinic establishment. What is known about the topic? Allied health clinics have been demonstrated to result in high patient, referrer and consultant satisfaction, and are a cost-effective management strategy for wait list demand. In Queensland, physiotherapy-led orthopaedic clinics have been operating since 2005. What does this paper add? This paper describes the establishment of 11 allied health primary contact models of care in speciality out-patient areas including Ear, Nose and Throat, Gynaecology, Urology, Neurology, Neurosurgery, Orthopaedics and Plastic Surgery, and involving speech pathologists, audiologists, physiotherapists, occupational therapists and podiatrists as primary contact practitioners. Observations of enablers for and challenges to implementation are presented as key lessons. What are the implications for practitioners? The new allied health primary contact models of care described in this paper should be considered by health service executives, allied health leaders and specialist out-patient departments as one strategy to address unacceptably long specialist wait lists.