RESUMO
Specimens belonging to the Anopheles maculipennis complex were collected as larvae or resting adults from May 2003 to November 2004 in the area of the Athens 2004 Olympic Rowing Centre in Schinias, Attiki, Greece, and identified by morphological and molecular analyses. Of the 201 specimens collected, 199 were found to be Anopheles sacharovi Favre and two were An. maculipennis Meigen s.s. on the basis of similarity to published sequence data for the rDNA internal transcribed spacer (ITS2) region and the mitochondrial cytochrome c oxidase I gene (COI). Sequence data from a number of specimens were obtained for both genes and compared with corresponding GenBank data derived from diverse geographical areas. A high degree of homology in ITS2 sequences was found in both species, ranging from 99.5% to 100% in An. sacharovi and 99.4% to 100% in An. Maculipennis, with no intraspecific variation in either of the two species in our study. The degree of homology in the COI sequences was 94.8-99.8% in An. sacharovi and 95.0-99.8% in An. maculipennis. The 522-bp fragment produced a rather high degree of intrapopulation polymorphism for An. sacharovi, generating nine different haplotypes, five of which were singletons. Intraspecific variation for these sequences ranged from 0.2% to 1.4%, but was much lower (0.77%) for the two An. maculipennis sequences. These findings represent the first characterization of the An. maculipennis complex in the area of Schinias.
Assuntos
Anopheles/genética , DNA Espaçador Ribossômico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Insetos Vetores/genética , Animais , Anopheles/classificação , Sequência de Bases , Primers do DNA/química , DNA Mitocondrial/química , DNA Ribossômico/química , Grécia , Insetos Vetores/classificação , Malária/prevenção & controle , Dados de Sequência Molecular , Polimorfismo de Fragmento de Restrição , Alinhamento de Sequência , Especificidade da EspécieRESUMO
DNA sequences were generated for eight specimens of the Anopheles maculipennis complex from Florina in NW Greece, and identified to species on the basis of comparison with ITS2 sequences for members of the complex already in GenBank. The sequences revealed the presence of An. maculipennis and An. messeae in Florina. Problems with sequence reliability and accessibility of sequences generated in earlier studies of Palaearctic members of the complex are discussed.
Assuntos
Anopheles/genética , DNA Espaçador Ribossômico/genética , Animais , Anopheles/química , Anopheles/classificação , Sequência de Bases , DNA Espaçador Ribossômico/química , Feminino , Grécia , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
Keys to the adult females and 4th instar larvae of the mosquitoes of Greece are presented. In all, 53 species in 7 genera are included. Also, Aedes albopictus is added because of the potential for its introduction into Greece.
Assuntos
Culicidae/classificação , Animais , Feminino , GréciaRESUMO
A study of the literature and mosquito collections in Greece has yielded 3 species that have not been recorded from Greece. They are Aedes annulipes, Aedes berlandi, and Culex pusillus. Notes on their recognition and geographic distribution are also given.