RESUMO
Trichopus zeylanicus Gaertn, (Trichopodaceae) is also known as "Arogyappacha" meaning the greener of health by tribal inhabitants (Kani tribes). This plant used as health tonic and rejuvenator. The whole plant material of Trichopus zeylanicus is defatted and successively extracted with methanol. The alkaloid fraction of Trichopus zeylanicus was obtained from methanol extract. Up to the dose of 2000 mg/kg b.w. per orally alkaloid fraction of Trichopus zeylanicus did not show any mortality or toxicity. Immunomodulatory activity of alkaloid fraction of Trichopus zeylanicus Gaertn was evaluated using various in vivo models including neutrophil adhesion test, delayed type hypersensitivity reaction, and effect on hematological parameter like, total white blood cell's, red blood cell's and hemoglobin and cyclophosphamide induce immunosupression. Sheep red blood cells were used to immunized the animals. The percentage of neutrophils adhesion to the nylon fiber was dose dependently increased in alkaloid fraction of Trichopus zeylanicus75, 150 and 300 mg/kg, p.o treated groups (50.57, 52.99 and 54.21%), respectively compared to control group. A dose dependent potentiating of delayed type hypersensitivity reaction induced by sheep red blood cells was also observed from the alkaloid fraction of Trichopus zeylanicus. On chronic administration of alkaloid fraction of Trichopus zeylanicus (75, 150 and 300 mg/kg. p.o.) caused significant (P<0.001) increased in hematological parameter like, total white blood cell's, red blood cell's and hemoglobin. Alkaloid fraction of Trichopus zeylanicus also prevented the myelosupression in mice treated cyclophosphamide (30 mg/kg, p.o.). The result of present investigation suggested that alkaloid fraction of Trichopus zeylanicus stimulate defense system by modulating several immunological parameters.
RESUMO
The extract of Chloroxylon sweitenia (Family: Rutaceae) leaves were investigated for its anti-inflammatory activity at the different doses in the standard animal models. The experimental paradigms used were carrageenan induced rat paw oedema (acute), and cotton pellet induced granuloma (chronic) models in rats for anti-inflammatory activity. In rats the toxicity was also performed for the extract by oral administration. The chloroform extract of Chloroxylon sweitenia (CECS) exhibited significant anti-inflammatory effect at the dose 50, 100 and 200 mg/kg. Maximum inhibition (55.32 %) was noted at the dose of 200 mg/kg after 3 h of drug treatment in carrageenan induced paw oedema, whereas the Diclofenac (standard drug) produced 61.33 % of inhibition. In the chronic model (cotton pellet induced granuloma) the CECS (200 mg/kg) and standard drug showed decreased formation of granuloma tissue by 52.32 % and 56.32 % (p < 0.001) respectively. The CECS further evaluated for their toxicity effect at the doses of 100 mg/kg administered for 14 days to orally in rats. At the end of experiments the blood, liver function and kidney metabolism was observed. The effect of CECS was assessed by the change in the body weight, lipid peroxidation and glutathione content (GSH) activities were measured from hepatic tissues. The hematological profile and different biochemical parameters such as SGOT, SGPT, and ALP were also estimated. Thus, the present study revealed that the chloroform extract of Chloroxylon sweitenia exhibited significant anti-inflammatory activity in the tested models Toxicity study indicates that the extract is non-toxic at the tested doses.
