RESUMO
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand, with the human papillomavirus (HPV) type-11 being the most widespread. In this study, we isolated the HPV type-11 (HPV-11) genome from subjects with RRP and subcloned the E6 and L2 open reading frames (ORFs) with the expression vectors pEX1 and pEX3. The recombinant E6/beta-gal and L2/beta-gal fusion proteins were expressed in E. coli. Using the recombinant proteins, we demonstrated the presence of antibodies against HPV-11 E6 and L2 in RRP patients by Western blot analysis. The prevalence of seropositivity for HPV-11 E6 and L2 were 5% (1/20) and 10% (2/20), respectively. Although RRP is caused by infection on the mucosal surface, it appears that an immune response occurs against viral proteins expressed in the epithelial lesions.
Assuntos
Anticorpos Antivirais/sangue , Neoplasias Laríngeas/diagnóstico , Recidiva Local de Neoplasia/diagnóstico , Proteínas Oncogênicas Virais/imunologia , Papillomaviridae/imunologia , Infecções por Papillomavirus/diagnóstico , Infecções Tumorais por Vírus/diagnóstico , Western Blotting , Criança , Pré-Escolar , Clonagem Molecular , Sondas de DNA de HPV , Feminino , Regulação Viral da Expressão Gênica/fisiologia , Humanos , Lactente , Neoplasias Laríngeas/imunologia , Masculino , Recidiva Local de Neoplasia/imunologia , Proteínas Oncogênicas Virais/genética , Papillomaviridae/genética , Infecções por Papillomavirus/imunologia , Tailândia , Infecções Tumorais por Vírus/imunologiaRESUMO
The problems with the system for reconstructive surgery for head and neck cancers in our department where plastic surgeons are regularly invited from distant facilities were discussed. Especially, the importance of preoperative discussion between the plastic surgeons and ENT doctors was stressed. The computer system with Internet will play an important role in exchanging information such as CT scans and MRI scans between the plastic surgeons and ENT doctors. Moreover, the establishment of a system to prevent postoperative troubles such as closure of anastomosed vessels was stressed. Twenty-seven re-vascularized free flaps had been transferred with a 96.3% success rate (26 of 27 cases). We concluded that the system for reconstructive surgery under the multiple facility cooperation system worked well.
Assuntos
Neoplasias de Cabeça e Pescoço/cirurgia , Equipe de Assistência ao Paciente , Cirurgia Plástica , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Relações Interprofissionais , Masculino , Pessoa de Meia-Idade , Retalhos CirúrgicosRESUMO
A 32-year-old female with bilateral congenital stapes fixation accompanied by bilateral proximal symphalangism and bilateral hypermetropia is reported. This is the 19th case of congenital stapes fixation and symphalangism in Japan. Hypermetropia was speculated to be one of the cardinal symptoms of the disease based on the present case and cases previously reported. In this case hypermetropia was due to pure microphthalmos and this was the first report that revealed the cause of hypermetropia in the syndrome. Bilateral stapedotomy were carried out and her hearing loss was improved to the satisfactory level.