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Significance: Label-free quantitative phase imaging can potentially measure cellular dynamics with minimal perturbation, motivating efforts to develop faster and more sensitive instrumentation. We characterize fast, single-shot quantitative phase gradient microscopy (ss-QPGM) that simultaneously acquires multiple polarization components required to reconstruct phase images. We integrate a computationally efficient least squares algorithm to provide real-time, video-rate imaging (up to 75 frames / s ). The developed instrument was used to observe changes in cellular morphology and correlate these to molecular measures commonly obtained by staining. Aim: We aim to characterize a fast approach to ss-QPGM and record morphological changes in single-cell phase images. We also correlate these with biochemical changes indicating cell death using concurrently acquired fluorescence images. Approach: Here, we examine nutrient deprivation and anticancer drug-induced cell death in two different breast cell lines, viz., M2 and MCF7. Our approach involves in-line measurements of ss-QPGM and fluorescence imaging of the cells biochemically labeled for viability. Results: We validate the accuracy of the phase measurement using a USAF1951 pattern phase target. The ss-QPGM system resolves 912.3 lp / mm , and our analysis scheme accurately retrieves the phase with a high correlation coefficient ( â¼ 0.99 ), as measured by calibrated sample thicknesses. Analyzing the contrast in phase, we estimate the spatial resolution achievable to be 0.55 µ m for this microscope. ss-QPGM time-lapse live-cell imaging reveals multiple intracellular and morphological changes during biochemically induced cell death. Inferences from co-registered images of quantitative phase and fluorescence suggest the possibility of necrosis, which agrees with previous findings. Conclusions: Label-free ss-QPGM with high-temporal resolution and high spatial fidelity is demonstrated. Its application for monitoring dynamic changes in live cells offers promising prospects.
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Algoritmos , Humanos , Processamento de Imagem Assistida por Computador/métodos , Linhagem Celular Tumoral , Microscopia de Contraste de Fase/métodos , Células MCF-7 , Microscopia de Fluorescência/métodosRESUMO
Calcification of aortic valve leaflets is a growing mortality threat for the 18 million human lives claimed globally each year by heart disease. Extensive research has focused on the cellular and molecular pathophysiology associated with calcification, yet the detailed composition, structure, distribution and etiological history of mineral deposition remains unknown. Here transdisciplinary geology, biology and medicine (GeoBioMed) approaches prove that leaflet calcification is driven by amorphous calcium phosphate (ACP), ACP at the threshold of transformation toward hydroxyapatite (HAP) and cholesterol biomineralization. A paragenetic sequence of events is observed that includes: (1) original formation of unaltered leaflet tissues: (2) individual and coalescing 100's nm- to 1 µm-scale ACP spherules and cholesterol crystals biomineralizing collagen fibers and smooth muscle cell myofilaments; (3) osteopontin coatings that stabilize ACP and collagen containment of nodules preventing exposure to the solution chemistry and water content of pumping blood, which combine to slow transformation to HAP; (4) mm-scale nodule growth via ACP spherule coalescence, diagenetic incorporation of altered collagen and aggregation with other ACP nodules; and (5) leaflet diastole and systole flexure causing nodules to twist, fold their encasing collagen fibers and increase stiffness. These in vivo mechanisms combine to slow leaflet calcification and establish previously unexplored hypotheses for testing novel drug therapies and clinical interventions as viable alternatives to current reliance on surgical/percutaneous valve implants.
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Valva Aórtica , Calcinose , Fosfatos de Cálcio , Colágeno , Osteopontina , Fosfatos de Cálcio/metabolismo , Humanos , Valva Aórtica/metabolismo , Valva Aórtica/patologia , Osteopontina/metabolismo , Calcinose/metabolismo , Calcinose/prevenção & controle , Colágeno/metabolismo , Durapatita/metabolismo , Durapatita/química , Estenose da Valva Aórtica/metabolismo , Estenose da Valva Aórtica/patologia , Colesterol/metabolismoRESUMO
Streptomyces avermitilis is a gram-positive bacterium that undergoes complex physiological and morphological differentiation during its life cycle, which has implications in secondary metabolite production. Avermectin, produced by S. avermitilis, is widely used as an anthelmintic and insecticidal agent. In this study, we have applied Raman microspectroscopic imaging to elucidate the correlation between production of avermectin and the morphological differentiation in S. avermitilis. We demonstrate distinctive variations in the localization of secondary metabolites at various stages of morphological differentiation. Under solid culture, avermectin was detected in the mycelia formed at the later stages of morphological differentiation (e.g., spore-bearing mycelium and spiral spore chains), but not in the early-stage substrate mycelium. On the contrary, under liquid culture condition, avermectin was found concentrated in the mycelial pellet formed at the early MII stage of differentiation. Furthermore, the chemical profiles of the mycelia were substantially different depending on the culture condition. Raman spectra corresponding to proteins, lipids, and cytochrome were observed in the mycelia irrespective of the stage of morphological differentiation, however, carotenoid was observed under solid culture condition particularly in spore-bearing mycelium and spiral spore chains. KEY POINTS: ⢠Avermectin production is regulated during mycelial differentiation ⢠Liquid and solid culture conditions affects mycelial differentiation ⢠Raman microspectroscopic analysis reveals localization profiles of avermectin.
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Regulação Bacteriana da Expressão Gênica , Streptomyces , Streptomyces/metabolismo , Ivermectina , Micélio/metabolismoRESUMO
Lipids, proteins, and nucleic acids have closely associated water molecules (Bound water), which exhibit considerably different physical properties compared to bulk water. Here we investigate the possibility of resolving Raman spectra of the specific hydration shell of these biomolecules in intracellular regions using Raman imaging. Lipids and proteins + nucleic acids Raman spectral components resolved in the analysis showed associated water spectral features, which are uniquely different from that of bulk water. These spectral profiles agree with water spectral profile observed in the case of corresponding hydrated pure biomolecules. The results show the prospects of Raman imaging in examining intracellular hydration in biomolecules and its functional relation.
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Ácidos Nucleicos , Análise Espectral Raman , Análise Espectral Raman/métodos , Água/química , LipídeosRESUMO
Methodologies for direct intracellular imaging of RNA and DNA are necessary for the advancement of bioimaging. Here we show direct label-free imaging of RNA and DNA in single cells by isolating their accurate Raman spectra. Raman images of DNA from interphase cells show intact nucleus, while those from mitotic cells reveal condensed chromosome. The condensed chromosome images are accurate enough to assign the stage of mitotic cell division (e.g., metaphase). Raman spectral features indicate B-DNA double helical conformational form in all the cell lines investigated here. The Raman images of RNAs, on the other hand, reveal liquid-liquid phase separated (LLPS) membraneless organelles in interphase cells, which disappears during mitosis. Further, the Raman spectrum of proteins from the intracellular LLPS organelles indicates slight enrichment of amyloid-like secondary structural features. Vibrational imaging of intracellular DNA and RNA simultaneously would open myriad of opportunities for examining functional biochemical aspects of cells and organelles.
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Condensados Biomoleculares , RNA , RNA/química , Núcleo Celular , DNA , MitoseRESUMO
Spatial transcriptome analysis of formalin-fixed paraffin-embedded (FFPE) tissues using RNA-sequencing (RNA-seq) provides interactive information on morphology and gene expression, which is useful for clinical applications. However, despite the advantages of long-term storage at room temperature, FFPE tissues may be severely damaged by methylene crosslinking and provide less gene information than fresh-frozen tissues. In this study, we proposed a sensitive FFPE micro-tissue RNA-seq method that combines the punching of tissue sections (diameter: 100 µm) and the direct construction of RNA-seq libraries. We evaluated a method using mouse liver tissues at two years after fixation and embedding and detected approximately 7000 genes in micro-punched tissue-spots (thickness: 10 µm), similar to that detected with purified total RNA (2.5 ng) equivalent to the several dozen cells in the spot. We applied this method to clinical FFPE specimens of lung cancer that had been fixed and embedded 6 years prior, and found that it was possible to determine characteristic gene expression in the microenvironment containing tumor and non-tumor cells of different morphologies. This result indicates that spatial gene expression analysis of the tumor microenvironment is feasible using FFPE tissue sections stored for extensive periods in medical facilities.
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MicroRNAs , Camundongos , Animais , Inclusão em Parafina/métodos , Fixação de Tecidos/métodos , Formaldeído , Análise de Sequência de RNA/métodos , Perfilação da Expressão Gênica/métodos , Transcriptoma , RNA/genética , RNA/análiseRESUMO
The mechanism of production of extracellular vesicles (EVs) and their molecular contents are of great interest due to their diverse roles in biological systems and are far from being completely understood. Even though cellular cargo releases mediated by EVs have been demonstrated in several cases, their role in secondary metabolite production and release remains elusive. In this study, this aspect is investigated in detail using Raman microspectroscopic imaging. Considerable evidence is provided to suggest that the release of antibiotic penicillin by the filamentous fungus Penicillium chrysogenum involves EVs. Further, the study also reveals morphological modifications of the fungal body during biogenesis, changes in cell composition at the locus of biogenesis, and major molecular contents of the released EVs. The results suggest a possible general role of EVs in the release of antibiotics from the producing organisms.
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Vesículas Extracelulares , Penicillium chrysogenum , Vesículas Extracelulares/metabolismo , Penicilinas , Penicillium chrysogenum/metabolismoRESUMO
Light transmission aggregometry (LTA) is the gold standard for the diagnosis of platelet function disorders (PFDs). The requirement of customized aggregometer, large blood volume, normal platelet count and processing within 4 hours of venipuncture for LTA makes platelet function testing inaccessible to wider population. Flow cytometric platelet activation test (PACT) may overcome these limitations. This study compares the performance of PACT with LTA, characterizes diagnostic patterns of PFDs on PACT and assesses the stability of PACT beyond 4 hours of venipuncture in controls (n = 5) at different temperature conditions. LTA and PACT were performed in 121 healthy controls and 66 patients with suspected PFD. PACT had excellent agreement (kappa = 0.93) with LTA and 94.1% sensitivity, 98.5% specificity. PACT had distinct patterns in Bernard Soulier Syndrome (n = 10), Glanzmann Thrombasthenia (n = 24), δ-granule disorder (n = 7), and other PFDs (n = 12). PACT could assess platelet function in patients (14%) with thrombocytopenia/lipemia wherein LTA was inconclusive. PACT was stable up to 24 hours in samples stored/transported at 2-8â¦C. The results of utility and stability are only valid for the specific markers, agonist concentrations, and conditions investigated in this paper. PACT is a useful modality for the diagnosis of PFD, especially in children, thrombocytopenia cases or in the setup where an aggregometer is not readily available.
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Transtornos Plaquetários , Trombocitopenia , Plaquetas , Criança , Humanos , Ativação Plaquetária , Agregação Plaquetária , Testes de Função Plaquetária/métodosRESUMO
Raman imaging has transcended in recent times from being an analytical tool to a molecular profiling technique. Biomedical applications of this technique often rely on singular-value decomposition (SVD), principal component analysis (PCA), etc. for data analysis. These methods, however, obliterate the molecular information contained in the original Raman data leading to speculative interpretations based on relative intensities. In the present study, SVD analysis of the Raman images from Penicillium chrysogenum resulted in 11 spectral components and corresponding images with highly distorted spectral features and complex image contrast, respectively. To interpret the SVD results in molecular terms, we have developed a combined multivariate approach. By applying this methodology, we have successfully extracted the contribution of five biomolecular constituents of the P. chrysogenum filamentous cell to the SVD vectors. Molecular interpretability will help SVD/PCA surpass the realm of variance-based classification to a more meaningful molecular domain.
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Análise de Componente PrincipalRESUMO
Raman spectra are molecular structure-specific and hence are employed in applications requiring chemical identification. The advent of efficient handheld and smartphone-based Raman instruments is promoting widespread applications of the technique, which often involve less trained end users. Software modules that enable spectral library searches based on spectral pattern matching is an essential part of such applications. The Raman spectrum recorded by end users will naturally have varying levels of signal to noise (SN), baseline fluctuations, etc., depending on the sample environment. Further, in biological, forensic, food, pharmaceuticals, etc., fields where a vast amount of Raman spectral data is generated, careful removal of background is often impossible. In other words, a 100% match between the library spectrum and user input cannot be often guaranteed or expected. Often, such influences are discounted upon developing mathematical methods for general applications. In this manuscript, we carefully examine how such effects would determine the results of spectral similarity-based library search. We show that several popular mathematical spectral matching approaches give incorrect results under the influence of small changes in the baseline and/or the noise. We also discuss the points to be carefully considered while generating a spectral library. We believe our results will be a guiding note for developing applications of Raman spectroscopy that uses a standard spectral library and mathematical spectral matching.
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The genus Okeania is a globally distributed group of microorganisms that live in shallow seabed regions. These organisms play several environmentally important roles and are also known producers of several active secondary metabolites with potential human applications. Here, we present a draft genome of Okeania sp. strain KiyG1 (92.7% completeness) that was assembled from four single-amplified genomes.
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Rickettsiales-like organisms are important for the survival and functioning of corals, prompting an investigation of their complete genomes. Earlier reports of the genomes of these organisms remain incomplete. Here, we report a novel draft genome of Rickettsiales bacterial strain SESOKO1, found in Acropora tenuis coral, using single-cell genome technology.
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Raman microspectroscopy is a minimally invasive technique that can identify molecules without labeling. In this study, we demonstrate the detection of penicillin G inside Penicillium chrysogenum KF425 fungal cells. Raman spectra acquired from the fungal cells had highly overlapped spectroscopic signatures and hence were analyzed with multivariate curve resolution by alternating least-squares (MCR-ALS) to extract the spectra of individual molecular constituents. In addition to detecting spatial distribution of multiple constituents such as proteins and lipids inside the fungal body, we could also observe the subcellular localization of penicillin G. This methodology has the potential to be employed in screening the production of bioactive compounds by microorganisms.
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Penicilina G/metabolismo , Penicillium chrysogenum/metabolismo , Análise Espectral Raman/métodos , Cromatografia Líquida de Alta Pressão/métodos , Fermentação , Análise dos Mínimos Quadrados , Análise MultivariadaRESUMO
Raman imaging has become an attractive technology in molecular biology because of its ability to detect multiple molecular components simultaneously without labeling. Two major limitations in accurately accounting for spectral features, viz., background removal and spectral unmixing, have been overcome by employing a modified and effective routine in multivariate curve resolution (MCR). With our improved strategy, we have spectrally isolated seven structurally specific biomolecules without any post-acquisition spectral treatments. Consequently, the isolated intensity profiles reflected concentrations of corresponding biomolecules with high statistical accuracy. Our study reveals the changes in the molecular composition of lipid droplets (LDs) inside HuH7 cells and its relation to the physiological state of the cell. Further, we show that the accurate separation of spectral components permits analysis of structural modification of molecules after cellular uptake. A detailed discussion is presented to highlight the potential of Raman spectroscopy with MCR in semi-quantitative molecular profiling of living cells.
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Gotículas Lipídicas/química , Fígado/química , Células Cultivadas , Colesterol/análise , Humanos , Fígado/citologia , Ácido Oleico/análise , Análise Espectral RamanRESUMO
In Japan, the imports of meat products have been increasing every year. Heat processing of meat is the current standard method for ensuring domestic animal health, particularly in case of meat products from areas where infectious diseases are known to have occurred in domestic animals. The Animal Quarantine Service needs to establish a method that detects the temperature at which the meat has been heat-processed (endpoint temperature) to ensure that the standard protocol is followed at the production location. Here, we developed a Raman spectroscopy and multivariate statistics (viz. multivariate curve resolution (MCR))-based simple and rapid method for accurately estimating the end point temperature. We showed that the temperature-dependent secondary structure modification of proteins can serve as an accurate indicator of the temperature of heat processing. This methodology can be easily automated for effective utilization by someone who is not an expert in spectroscopy. We envisage a wider application of this method in food analysis, although the present research investigated the application of this method in chicken meat heat processing analysis.
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Análise de Alimentos/métodos , Carne/análise , Análise Espectral Raman/métodos , Animais , Galinhas , Temperatura AltaRESUMO
Physical properties of polymers (e.g. crystallinity, lamella thickness, thermodynamic properties etc.) can in principle be reliably estimated from their Raman spectral intensities by converting intensities to corresponding concentrations of conformers. However, such conversions are not straightforward due to the unknown scattering cross-sections. The study demonstrates that for several practical applications of Raman spectroscopy, a ratio of cross-sections can be used instead of the absolute values. A straight forwards method for accurately estimating ratio of scattering cross-section from variable temperature measurements is described here. In order to demonstrate its applicability, percent crystallinity (PC) of polyethylene has been directly estimated from Raman intensities without external calibration with other techniques. This general method can be applied to any polymer when there is a continuous change in composition of conformers over a range of temperatures.
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A general method for estimating lamella-thickness distribution in semicrystalline polymers has been developed and applied to polyethylene (PE). The longitudinal acoustic mode (LAM) of PE appears at very low frequencies (i.e., νË 8-20â cm-1 ) in the Raman spectrum. It represents a distribution of lamellae of varying thicknesses. We propose a distribution function that converts a low-frequency LAM Raman band into the corresponding lamellae-thickness distribution. By using this distribution function, we can study lamella formation in crystallizing PE to elucidate the influence of supercooling and determine critical lamella thickness, the minimum chain length at which folding occurs, and the associated thermodynamic parameters accurately. This method has a general applicability toward the examination of polymer crystallization in an accurate and straightforward manner. Understanding the molecular details of polymer crystallization has applications, particularly in polymer thin-film photovoltaics and polymer processing, beyond its fundamental academic significance.
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A low generation amphiphilic dendrimer, POSS-AD, which has a POSS core and eight amphiphilic arms, was synthesized and used as a nano-reactor to produce well-defined polymer nano-cylinders. Confirmed by small-angle X-ray scattering (SAXS), Raman and NMR spectrometry, monodispersed cylindrical micelles that contain a hydrophilic cavity with a diameter of 2.09 nm and a length of 4.26 nm were produced via co-assembling POSS-AD with hydrophilic liquids, such as H2O and HEMA in hydrophobic solvents. Taking the HEMA/POSS-AD cylindrical micelles as nano-reactors, polymerization of HEMA within the micelles results in polymer nano-cylinders (POSS-ADNPs) with a diameter of 2.24 nm and a length of 5.02 nm. The study confirmed that despite the inability to maintain specific shape in solution, low generation dendrimers form well-defined nano-containers or nano-reactors, which relies on co-assembling with hydrophilic guest molecules. These nano-reactors are robust enough to maintain their shape during the polymerization of the guest molecules. Polymer nano-cylinders with dimensions less than 10 nm can thus be produced from the HEMA/POSS-AD micelles. Since the chemical structure of low-generation dendrimers and the contents of the co-assembled nano-reactors can be easily adjusted, the concept holds the potential for the further developments of low-generation amphiphilic dendrimers.
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We have recently demonstrated a methodology to estimate the percent crystallinity (PC) of polymers directly with Raman spectroscopy and multivariate curve resolution (MCR) by alternating least-squares (ALS). In the MCR-ALS methodology, the Raman spectrum of a semicrystalline polymer is separated into two constituent components (crystalline and molten/amorphous) and their corresponding concentrations. The methodology necessitates that the Raman spectrum at any temperature be a linear combination of two MCR spectral components (one molten and one crystalline). This is true in the case of simple systems such as crystalline pendant alkyl domains in polymers (Samuel et al. Anal. Chem. 2016, 88, 4644). However, in the case of main chain polymer crystals (e.g., polyethylene), the situation can be complicated owing to several molecular changes in the lattice in addition to conformational reorganizations during melting. Under this circumstance, a simple two-state model may not be adequate and we describe the modifications required to treat such systems, keeping the basic principles of the proposed methodology unchanged. A comparative study with wide-angle X-ray scattering (WAXS) and Raman spectroscopy is also performed to substantiate our findings. In addition to estimating percent crystallinity (PC), our methodology is capable of revealing additional information, such as interchain interactions in crystal lattice, that in principle will help distinguishing polymorphic transformations, subtle changes in lamellar lattice dimensions, and other phase changes in polymers.