[The influence of low-intensity laser irradiation on the loach embryo cells].

The ultrastructural organization of loach embryo cells (Misgurnus fossilis L) at the stage of the first and the tenth embryo divisions was investigated in the control and under the influence of low intensity helium-neon laser irradiation of 5 min exposure. The effect of laser irradiation led to ultrastructural changes in cell organelles, increasing the number and size of mitochondria, and as a result their shape changes. Under the influence of laser irradiation, the activation of cellular digestion processes took place, the number of vacuoles and lysosomes increased. The results explain the possible mechanism laser irradiation impact at the cellular level.

[The influence of potassium 4-toluenethiosulfonate on membrane potential and ATPase activity of plasmatic membranes of loach embryos].

The influence of novel biologically active substance potassium 4-toluenethiosulfonate in concentration of 4 x 10(-5) M on the changes of electrophysiological parameters of embryonic cells in early development of fish (Misgurnus fossilis L.) was investigated including the changes of membrane potential (TMP) and enzyme activity of plasmatic membranes of loach embryos during the period of synchronous division of blastomers in the early period of development. The evaluation of influence of these matters was studied and aperiodic changes of the level of TMP were shown. The diminishing of amplitude in every period by 7/12 mV and diminishing of growth of maximal values of vibrations of TMP by 39 mV in comparison to control was noticed. It was related to inhibition of some biosynthetic processes and results in the decline of activity of membrane enzyme (Na+, K(+)-ATPase) by 75.5 +/- 4.1% and 78.4 +/- 10.4% both at the action of high (4 x 10(-3) M) and low (4 x 10(-9) M) concentrations, accordingly, at first hours of development with subsequent renewal of its activity to the level of control only for the actions of low concentration.