Identification of COQ2 as a regulator of proliferation and lipid peroxidation through genome-scale CRISPR-Cas9 screening in myeloma cells.

Multiple myeloma (MM) is the second most common malignant haematological disease with a poor prognosis. The limit therapeutic progress has been made in MM patients with cancer relapse, necessitating deeper research into the molecular mechanisms underlying its occurrence and development. A genome-wide CRISPR-Cas9 loss-of-function screening was utilized to identify potential therapeutic targets in our research. We revealed that COQ2 plays a crucial role in regulating MM cell proliferation and lipid peroxidation (LPO). Knockout of COQ2 inhibited cell proliferation, induced cell cycle arrest and reduced tumour growth in vivo. Mechanistically, COQ2 promoted the activation of the MEK/ERK cascade, which in turn stabilized and activated MYC protein. Moreover, we found that COQ2-deficient MM cells increased sensitivity to the LPO activator, RSL3. Using an inhibitor targeting COQ2 by 4-CBA enhanced the sensitivity to RSL3 in primary CD138+ myeloma cells and in a xenograft mouse model. Nevertheless, co-treatment of 4-CBA and RSL3 induced cell death in bortezomib-resistant MM cells. Together, our findings suggest that COQ2 promotes cell proliferation and tumour growth through the activation of the MEK/ERK/MYC axis and targeting COQ2 could enhance the sensitivity to ferroptosis in MM cells, which may be a promising therapeutic strategy for the treatment of MM patients.

Metal-organic-framework derived Zn-V-based oxide with charge storage mechanism as high-performance anode material to enhance lithium and sodium storage.

Transition metal oxides have been extensively studied due to their large theoretical capacities, but their practical application has been hampered by low electrical conductivity and dramatic volume fluctuation during cycling. In this work, we synthesized Zn3V2O8 material using Zn-V-MOF (metal-organic framework) as a sacrificial template to improve the electrochemical characteristics of lithium-ion batteries (LIBs) and sodium-ion batteries (SIBs). Unique dodecahedral structure, larger specific surface area and higher ability to mitigate volume changes, improve the electrochemical reaction active site while accelerating ion transport. Zn3V2O8 with 2-methylimidazole as a ligand demonstrated a discharge capacity of 1225.9 mAh/g in LIBs and 761.6 mAh/g in SIBs after 300 cycles at 0.2 C. Density functional theory (DFT) calculation illustrates the smaller diffusion barrier energy and higher specific capacity in LIBs that is ascribed to the fact that Li has a smaller size and hence its diffusion is easier. This study may lead to a path for the manufacturing of high-performance LIBs and SIBs.

Detection of heavy metal ion in real samples with fiber based paper based on new rare earth cluster.

Chromium (Cr) is an important material, but also one of the most toxic heavy metal pollutants, showing great threat to human health and ecological environment, thus, accurate and rapid detection of Cr3+ has far-reaching significance. In this work, based on the ligand of 2,3,4,5,6-pentafluorobenzoic acid (HPFBA) that does not contains oscillation effect group such as "CH, OH, and NH bond", three rare earth dinuclear cluster of Ln2(PFBA)6(phen)2(H2O)2 (Ln = Tb3+1-Tb, Eu3+1-Eu, Gd3+1-Gd, phen = 1,10-phenanthroline) were obtained. 1-Tb shows excellent stability and luminescence properties. In depth investigation reveals that 1-Tb shows quick detection towards Cr3+ in water through luminescence "turn-off", with extremely short response time of 1.0 min, very low limit of detection (LOD) of 5.2 ppb and no interference from other ions. The LOD value is much lower than the total content of chromium for water in China (15 ppm, GB9078-1996). In the actual environment such as tap water, lake water, human, and serum, 1-Tb shows excellent detection and recovery rate for Cr3+. More interestingly, a fiber based paper of test paper that based on 1-Tb and ordinary filter paper was fabricated, which can probe Cr3+ by visible color changes to the naked eye under UV light.

Effects of functional training on the athletic quality of aerobics practitioners / Efeitos do treinamento funcional sobre a qualidade atlética dos praticantes de aeróbica / Efectos del entrenamiento funcional en la calidad atlética de los practicantes de aeróbic

ABSTRACT Introduction: Athletes' level of physical fitness has become the basis for achieving their sports ability. The lack of physical fitness can generate a great impasse in the quality of movements and the performance of the athletes. Objective: Study functional training research on aerobics athletes' athletic quality. Methods: The content of functional training applications in competitive aerobics and fitness was analyzed through a literature review. Research methods of mathematical statistics were used to analyze the collected data and contrast it with the current scientific literature. Volunteers for the research were athletes from the aerobics team at the Institute of Physical Education. They were randomly divided into two groups, the experimental group (n=9) and the control group (n=9). Functional training was added only to the experimental group; all performed the standard training. Results: In the mean total score of the FMS functional movement screening test, the athletes in the experimental group from 9.22 points before the experiment to 15.65 points after the experiment. The control group athletes improved from 9.83 points before the experiment to only 11.23 points after. Conclusion: It was evidenced a dominant improvement in the movement of the athletes who executed the suggested training, acquiring greater coordination gain through functional training. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O nível de aptidão física dos atletas se converteu na base para atingir sua habilidade esportiva. A falta de aptidão física pode gerar um grande impasse na qualidade de movimentos e no desempenho dos atletas. Objetivo: Estudar a pesquisa do treinamento funcional sobre a qualidade atlética de atletas de aeróbica. Métodos: Analisou-se o conteúdo da aplicação do treinamento funcional na aeróbica competitiva e na aptidão física através da revisão da literatura. Métodos de pesquisa de estatísticas matemáticas foram utilizadas para analisar os dados coletados e contrastar com a literatura científica vigente. Foram voluntários da pesquisa os atletas da equipe de aeróbica do Instituto de Educação Física. Divididos aleatoriamente em dois grupos, grupo experimental (n=9) e o grupo controle (n=9). O treinamento funcional foi adicionado apenas ao grupo experimental, todos executaram o treinamento padrão. Resultados: Na pontuação média da pontuação total do teste FMS de triagem do movimento funcional, os atletas do grupo experimental, de 9,22 pontos antes do experimento para 15,65 pontos após o experimento. Os atletas do grupo controle melhoraram de 9,83 pontos antes do experimento para apenas 11,23 pontos após o experimento. Conclusão: Evidenciou-se uma melhoria dominante no movimento dos atletas que executaram o treinamento sugerido, adquirindo maior ganho de coordenação pelo treinamento funcional. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El nivel de aptitud física de los atletas se ha convertido en la base para alcanzar su capacidad deportiva. La falta de aptitud física puede generar un gran impasse en la calidad de los movimientos y en el rendimiento de los deportistas. Objetivo: Estudiar la investigación del entrenamiento funcional sobre la calidad atlética de los atletas de aeróbic. Métodos: Se analizó el contenido de la aplicación del entrenamiento funcional en el aeróbic de competición y la aptitud física mediante la revisión de la literatura. Se utilizaron métodos de investigación de estadística matemática para analizar los datos recogidos y contrastarlos con la literatura científica actual. Los voluntarios de la investigación fueron los atletas del equipo de aeróbic del Instituto de Educación Física. Se dividieron aleatoriamente en dos grupos, el grupo experimental (n=9) y el grupo de control (n=9). El entrenamiento funcional se añadió sólo al grupo experimental, todos realizaron el entrenamiento estándar. Resultados: En la puntuación total media de la prueba de detección de movimientos funcionales FMS, los atletas del grupo experimental pasaron de 9,22 puntos antes del experimento a 15,65 puntos después del mismo. Los atletas del grupo de control mejoraron de 9,83 puntos antes del experimento a sólo 11,23 puntos después del mismo. Conclusión: Se evidenció una mejora dominante en el movimiento de los atletas que ejecutaron el entrenamiento sugerido, adquiriendo una mayor ganancia de coordinación por el entrenamiento funcional. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

I2-mediated Csp2-P bond formation via tandem cyclization of o-alkynylphenyl isothiocyanates with organophosphorus esters.

A novel, I2-mediated tandem cyclization of o-alkynylphenyl isothiocyanates with organophosphorus esters has been developed under mild conditions. Different kinds of 4H-benzo[d][1,3]thiazin-2-ylphosphonate could be synthesized in moderate to excellent yields. This method has the advantages of easy access to raw materials, free-metal catalyst, simple operation, high yield and high functional group tolerance.

Magnetic deep eutectic solvent-based dispersive liquid-liquid microextraction for enantioselectively determining chiral mefentrifluconazole in cereal samples via ultra-high-performance liquid chromatography.

A simple, rapid, and efficient pretreatment method of mefentrifluconazole enantiomers in cereal samples was established by dispersive liquid-liquid microextraction coupled with ultra-high-performance liquid chromatography-diode array detector (UHPLC-DAD). In this study, a novel ternary magnetic deep eutectic solvent (MDES) [octyltrimethylammonium bromide][cobalt chloride][acetic acid] was synthesized as the extractant. Acetic acid was used as the dispersant to promote the in situ dispersion of binary MDES [octyltrimethylammonium bromide][cobalt chloride]. The microdroplets of binary MDES containing mefentrifluconazole were separated by an external magnet. Device-assisted dispersion and time-consuming centrifugation steps were eliminated to ensure simplicity and rapidity of the pretreatment. Good linearity ranging from 0.01 µg g-1 to 2 µg g-1 was obtained. The extraction recovery varied from 82.9 to 95.0%. The limit of detection was 0.003 µg g-1. Finally, this established approach has been applied for the enantioselective detection of chiral mefentrifluconazole in corn, rice, wheat, millet, and sorghum samples.

Synthesis of thiazolo[2,3-b]quinazoline derivatives via base-promoted cascade bicyclization of o-alkenylphenyl isothiocyanates with propargylamines.

A highly efficient cascade bicyclization reaction of o-alkenylphenyl isothiocyanates with propargylamines has been developed, which affords a series of thiazolo[2,3-b]quinazolines in good to excellent yields by using K2CO3 as a base in MeCN at 80 °C. This method is transition-metal-free and operationally simple with broad functional group tolerance. Mechanistically, 6-exo-trig hydroamination followed by 5-exo-dig hydrothiolation was involved in this transformation.

A silver(i)-catalyzed cascade bicyclization strategy for synthesis of 5H-benzo[d]tetrazolo[5,1-b][1,3]thiazines.

A simple and efficient protocol for silver(i)-catalyzed tandem reaction of o-alkynylphenyl isothiocyanates with sodium azide has been developed, affording a series of 5H-benzo[d]tetrazolo[5,1-b][1,3]thiazines in moderate to good yields. In this transformation, a [3 + 2] cycloaddition reaction mechanism was involved and two new rings were formed in one pot.

New simple synthesis of ring-fused 4-alkyl-4H-3,1-benzothiazine-2-thiones: Direct formation from carbon disulfide and (E)-3-(2-aminoaryl)acrylates or (E)-3-(2-aminoaryl)acrylonitriles.

A new simple and efficient method to construct ring-fused 4-alkyl-4H-3,1-benzothiazine-2-thione derivatives has been developed from carbon disulfide and (E)-3-(2-aminoaryl)acrylates or (E)-3-(2-aminoaryl)acrylonitriles under mild conditions, without the need for a metal catalyst. The newly developed method tolerates a wide range of substrates in moderate to excellent yields. Moreover, this method is advantageous over previous ones for the easy synthesis of reactants.