RESUMO
CONTEXT: Nitric oxide (NO) is a diatomic molecule that has been implicated in tumor progression of oral squamous cell carcinoma (OSCC). However, the mechanism of NO affecting tumor growth and progression remains unknown. Tumor progression has been recently received considerable attention, and there is increasing evidence of correlation of tumor biology and behavior. AIMS: We aim to evaluate tissue NO of OSCC patients and correlate these findings with grade and stage of the OSCC patients. MATERIALS AND METHODS: To count tissue nitric oxide in normal and OSCC cases. To compare the findings of tissue nitric oxide between normal and OSCC cases. SUBJECTS AND METHODS: Forty-two fresh tissue specimens from the excisional biopsy of OSCC patients and 42 tissue samples of normal healthy mucosa using ultraviolet visible spectrophotometer. STATISTICAL ANALYSIS USED: Statistical analysis was done using Student's unpaired t-test and Tukey's post hoc analysis. RESULTS: Tissue NO level was higher in OSCC compared with control group (P < 0.01). There was an increase in NO levels with advanced clinical staging and with decreased differentiation of tumor. CONCLUSIONS: Increased tissue NO levels in OSCC patients along with an increase in the clinical stage of the tumor and decreased differentiation of tumor indicates the association of NO with tumor growth and with staging and grading of is well recognized.
RESUMO
CONTEXT: Estimation of the age is a procedure adopted by anthropologists, archeologists and forensic scientists. Different methods have been undertaken. However none of them meet the standards as Demirjian's method since 1973. Various researchers have applied this method, in both original and modified form (Chaillet and Demirjian in 2004) in different ethnic groups and the results obtained were not satisfactory. AIMS: To determine the applicability and accuracy of modified Demirjian's method of dental age estimation (AE) in 8-18 year old Tibetan young adults to evaluate the interrelationship between dental and chronological age and the reliability between intra- and inter observer relationship. SETTINGS AND DESIGN: Clinical setting and computerized design. SUBJECTS AND METHODS: A total of 300 Tibetan young adults with an age range from 8 to 18 years were recruited in the study. Digital panoramic radiographs (DPRs) were evaluated as per the modified Demirjian's method (2004). STATISTICAL ANALYSIS USED: Pearson correlation, paired t-test, linear regression analysis. RESULTS: Inter -and intraobserver reliability revealed a strong agreement. A positive and strong association was found between chronological age and estimated dental age (r = 0.839) with P < 0.01. Modified Demirjian method (2004) overestimated the age by 0.04 years (2.04 months)in Tibetan young adults. CONCLUSIONS: Results suggest that, the modified Demirjian method of AE is not suitable for Tibetan young adults. Further studies: With larger sample size and comparision with different methods of AE in a given population would be an interesting area for future research.