Autotrasplante renal en hipertensión renovascular secundaria a aneurisma de arteria renal / Renal autotransplantation in renovascular hypertension secondary to renal artery aneurysm

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Serology for hepatitis B and C, HIV and syphilis in the initial evaluation of diabetes patients referred for an external nephrology consultation.

INTRODUCTION: Information is available regarding the prevalence and incidence of positive microbiological serology results both in the general population and in patients on haemodialysis in Spain. Nevertheless, little information is known regarding patients with diabetes attended in external nephrology consultations. OBJECTIVE: To evaluate the percentage of patients with positive serology results for hepatitis B and C, Human Immunodeficiency Virus (HIV), and syphilis in the initial assessment of patients referred to diabetic nephropathy external consultations. PATIENTS AND METHOD: Retrospective study of 500 patients assessed over the course of 5 years with the diagnosis of diabetic nephropathy originating in external consultations and for which hepatitis B, C, HIV, and syphilis serology had been requested. RESULTS: With regard to hepatitis B virus, 0.4% of patients had chronic hepatitis B, 10.2% had overcome the hepatitis and were in recovery, and 4.2% had received the hepatitis B vaccine. As for hepatitis C, 2.4% of the patients studied had antibodies against hepatitis C. With regard to syphilis, 0.8% of patients had positive serological results. No patients had positive HIV serology results. CONCLUSIONS: Despite major methodological limitations, this would be the first study to evaluate the microbiological serology of diabetic nephropathy patients treated in external consultations.

Implication of Hsc70, PDI and integrin αvß3 involvement during entry of the murine rotavirus ECwt into small-intestinal villi of suckling mice.

In the present study, a homologous rotavirus, ECwt, infecting small intestinal villi isolated from ICR and BALB/c mice were used as a model for identifying cell-surface molecules involved in rotavirus entry. Small-intestinal villi were treated with anti-Hsc70, anti-PDI, anti-integrin ß3 or anti-ERp57 antibodies or their corresponding F(ab')2 fragments before inoculation with rotavirus ECwt, RRV or Wa. Pretreatment of villi decreased virus infectivity by about 50-100 % depending of the rotavirus strain, antibody structure and detection assay used. Similar results were obtained by treating viral inocula with purified proteins Hsc70, PDI or integrin ß3 before inoculation of untreated villi. Rotavirus infection of villi proved to be sensitive to membrane-impermeant thiol/disulfide inhibitors such as DTNB and bacitracin, suggesting the involvement of a redox reaction in infection. The present results suggest that PDI, Hsc70 and integrin ß3 are used by both homologous and heterologous rotaviruses during infection of isolated mouse villi.

Serologías B, C, VIH y sífilis en la primera valoración de pacientes con diabetes remitidos a consulta externa de Nefrología / Serology for Hepatitis B and C, HIV and syphilis in the initial evaluation of diabetes patients referred for an external Nephrology consultation

Antecedentes: En España hay datos sobre prevalencia e incidencia de serologías microbiológicas positivas tanto en la población general como en pacientes en hemodiálisis. Sin embargo, se conocen pocos datos sobre los pacientes con diabetes atendidos en consulta externa de Nefrología. Objetivo: Valorar el porcentaje de pacientes con serologías positivas para hepatitis B y C, virus de inmunodeficiencia humana (VIH) y sífilis en la valoración inicial de los pacientes remitidos para estudio a la consulta externa de nefropatía diabética. Pacientes y métodos: Estudio retrospectivo de las serologías solicitadas con el diagnóstico de nefropatía diabética con origen en consultas externas de 500 pacientes valorados durante 5 años a los que se les había solicitado serologías de la hepatitis B, C, VIH y sífilis. Resultados: Respecto al virus de la hepatitis B, el 0,4 % de los pacientes tenían hepatitis B crónica, el 10,2 % había pasado la hepatitis y estaban curados y el 4,2 % había recibido la vacuna de la hepatitis B. En cuanto a la hepatitis C, el 2,4 % de los pacientes estudiados tenían anticuerpos frente hepatitis C. Respecto a la sífilis, el 0,8 % de los pacientes tenían serologías positivas. Ningún paciente presentó serologías positivas para VIH. Conclusiones: Si bien con grandes limitaciones metodológicas, este sería el primer estudio donde se revisan las serologías microbiológicas de pacientes con nefropatía diabética atendidos en consulta externa (AU)

Introduction: Information is available regarding the prevalence and incidence of positive microbiological serology results both in the general population and in patients on haemodialysis in Spain. Nevertheless, little information is known regarding patients with diabetes attended in external nephrology consultations. Objective: To evaluate the percentage of patients with positive serology results for hepatitis B and C, Human Immunodeficiency Virus (HIV), and syphilis in the initial assessment of patients referred to diabetic nephropathy external consultations. Patients and method: Retrospective study of 500 patients assessed over the course of 5 years with the diagnosis of diabetic nephropathy originating in external consultations and for which hepatitis B, C, HIV, and syphilis serology had been requested. Results: With regard to hepatitis B virus, 0.4% of patients had chronic hepatitis B, 10.2% had overcome the hepatitis and were in recovery, and 4.2% had received the hepatitis B vaccine. As for hepatitis C, 2.4% of the patients studied had antibodies against hepatitis C. With regard to syphilis, 0.8% of patients had positive serological results. No patients had positive HIV serology results. Conclusions: Despite major methodological limitations, this would be the first study to evaluate the microbiological serology of diabetic nephropathy patients treated in external consultations (AU)

Hipernatremia transitoria e hipodipsia en paciente con insuficiencia renal / Transitory hypernatraemia and hypodipsia in a renal failure patient

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Mouse intestinal villi as a model system for studies of rotavirus infection.

Rotavirus replicates in the mature enterocytes lining the villi of the small intestine and the availability of an in vitro system for culturing these natural target cells would contribute to substantial advances in the understanding of the pathogenesis of rotavirus. A novel in vitro system was established for culturing isolated small intestinal villi from suckling mice, and the susceptibility of the villus cells to the wild-type murine rotavirus EDIM-Cambridge (ECwt) infection was assessed by immunocytochemistry staining and ELISA. Cell viability of cultured villi infected by rotavirus was estimated to be higher than 70% 16 h post-infection, whereas the accumulated rotavirus structural and non-structural antigen was found to reach a maximum value at 24 h post-infection. Terminal apoptosis was found in about 65% of villus cells 22 h post-infection as detected with either propidium iodide or Hoechst 33342 staining. Mock-infected villus cells exhibited a slight tendency toward more extensive chromatin fragmentation compared to their rotavirus-infected counterpart, mainly when caspase-3 activity was measured. Examination of villus cells by ELISA indicated that the amount of rotavirus structural antigen accumulated at 12 h post-infection was nearly the same regardless of the intestinal section (duodenum, jejunum and ileum) used. The isolation, culture and infection of small intestinal villi from suckling mice has led to the introduction of a useful model for rotavirus studies.