In vivo hydroquinone exposure alters circulating neutrophil activities and impairs LPS-induced lung inflammation in mice.

Hydroquinone (HQ) is an environmental contaminant which causes immune toxicity. In this study, the effects of exposure to low doses of HQ on neutrophil mobilization into the LPS-inflamed lung were investigated. Male Swiss mice were exposed to aerosolized vehicle (control) or 12.5, 25 or 50ppm HQ (1h/day for 5 days). One hour later, oxidative burst, cell cycle, DNA fragmentation and adhesion molecules expressions in circulating neutrophils were determined by flow cytometry, and plasma malondialdehyde (MDA) levels were measured by HPLC. Also, 1h later the last exposures, inflammation was induced by LPS inhalation (0.1mg/ml/10min) and 3h later, the numbers of leukocytes in peripheral blood and in the bronchoalveolar lavage fluid (BALF) were determined using a Neubauer chamber and stained smears; adhesion molecules expressed on lung microvessel endothelial cells were quantified by immunohistochemistry; myeloperoxidase (MPO) activity was measured in the lung tissue by colorimetric assay; and cytokines in the BALF were determined by ELISA. In vivo HQ exposure augmented plasma MDA levels and oxidative activity of neutrophils, but did not cause alterations in cell cycle and DNA fragmentation. Under these conditions, the number of circulating leukocytes was not altered, but HQ exposure reduced LPS-induced neutrophil migration into the alveolar space, as these cells remained in the lung tissue. The impaired neutrophil migration into BALF may not be dependent on reduced cytokines secretions in the BALF and lung endothelial adhesion molecules expressions. However, HQ exposure increased the expression of ß(2) and ß(3) integrins and platelet-endothelial cell adhesion molecule-1 (PECAM-1) in neutrophils, which were not further enhanced by fMLP in vitro stimulation, indicating that HQ exposure activates circulating neutrophils, impairing further stimulatory responses. Therefore, it has been shown, for the first time, that neutrophils are target of lower levels of in vivo HQ exposure, which may be considered in host defense in infectious diseases.

Características da exposição ocupacional a poeira sem marmorarias da cidade de São Paulo / Characteristics of occupational exposure to dust in the work environment of marble shops in the city of São Paulo

O estudo das características da exposição ocupacional a poeiras no beneficiamento de rochas ornamentais em marmorarias teve como base a aplicação das convenções adotadas pela American Conference of Governmental Industrial Hygienists (ACGIH), pela International Organization for Standardization (ISO) e pelo Comité Europeén de Normalisation (CEN) para a classificação de poeiras por faixa de tamanhos de partícula. Foram consideradas as relações entre as concentrações de poeira nos ambientes de trabalho, os tipos de rochas trabalhadas, as operações a úmido e a seco, as máquinas e as ferramentas utilizadas e os tamanhos das partículas suspensas no ar. Os ambientes de trabalho das marmorarias mostraram altas concentrações de poeira nas frações inalável, torácica e respirável, originadas pelas ferramentas utilizadas no setor de acabamento a seco. As concentrações de sílica chegaram a ser 16 vezes superiores ao valor do limite de exposição ocupacional de 0,05 mg/m3. A avaliação de uma marmoraria que adotou acabamento a úmido mostrou que a probabilidade das concentrações ambientais ultrapassarem os valores de referência ocupacionais pôde ser reduzida em até 99%.

This paper reports a study based on the application of conventions adopted by the American Conference of Governmental Industrial Hygienists (ACGIH), International Organization for Standardization (ISO) and Comité Europeén de Normalisation (CEN) for the classification of types of dust by particle size range. The occupational exposure characterization took into account the relations among dust concentrations at workplace, types of rocks worked on, wet and dry operations, machinery and tools used, and the size distribution of particles suspended in the air. The workplaces at the marble shops were characterized by high concentrations of dust in inhalable, thoracic and respirable fractions, produced by the tools used in the dry abrasive polishing sector. The silica concentrations were up to 16 times higher than the 0.05 mg/m3 occupational exposure limit recommended. The evaluation of a marble shop that made use of the wet abrasive polishing process showed that the probability of environmental concentrations exceeding the occupational reference values could be reduced by up to 99%.