Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 10 de 10
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Genes (Basel) ; 14(7)2023 07 14.
Artigo em Inglês | MEDLINE | ID: mdl-37510350

RESUMO

Classically, genetic association studies have attempted to assess genetic polymorphisms related to human physiology and physical performance. However, the heterogeneity of some findings drives the research to replicate, validate, and confirmation as essential aspects for ensuring their applicability in sports sciences. Genetic distance matrix and molecular variance analyses may offer an alternative approach to comparing athletes' genomes with those from public databases. Thus, we performed a complete sequencing of 44 genomes from male Brazilian first-division soccer players under 20 years of age (U20_BFDSC). The performance-related SNP genotypes were obtained from players and from the "1000 Genomes" database (European, African, American, East Asian, and South Asian). Surprisingly, U20_BFDSC performance-related genotypes had significantly larger FST levels (p < 0.00001) than African populations, although studies using ancestry markers have shown an important similarity between Brazilian and African populations (12-24%). U20_BFDSC were genetically similar to professional athletes, showing the intense genetic selection pressure likely to occur before this maturation stage. Our study highlighted that performance-related genes might undergo selective pressure due to physical performance and environmental, cognitive, and sociocultural factors. This replicative study suggests that molecular variance and Wright's statistics can yield novel conclusions in exercise science.


Assuntos
Desempenho Atlético , Futebol , Humanos , Masculino , Adolescente , Futebol/fisiologia , Brasil , Desempenho Atlético/fisiologia , Atletas , Exercício Físico
3.
Int J Sports Physiol Perform ; 15(8): 1156-1167, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32335533

RESUMO

PURPOSE: High cardiorespiratory capacity is a key determinant of human performance and life expectancy; however, the underlying mechanisms are not fully understood. The objective of this pilot study was to investigate biochemical signatures of endurance-performance athletes using high-resolution nontargeted metabolomics. METHODS: Elite long-distance runners with similar training and anthropometrical records were studied. After athletes' maximal oxygen consumption (V˙O2max) was measured, they were divided into 2 groups: low V˙O2max (<65 mL·kg-1·min-1, n = 7) and high V˙O2max (>75 mL·kg-1·min-1, n = 7). Plasma was collected under basal conditions after 12 hours of fasting and after a maximal exercise test (nonfasted) and analyzed by high-resolution LC-MS. Multivariate and univariate statistics were applied. RESULTS: A total of 167 compounds were putatively identified with an LC-MS-based metabolomics pipeline. Partial least-squares discriminant analysis showed a clear separation between groups. Significant variations in metabolites highlighted group differences in diverse metabolic pathways, including lipids, vitamins, amino acids, purine, histidine, xenobiotics, and others, either under basal condition or after the maximal exercise test. CONCLUSIONS: Taken together, the metabolic alterations revealed in the study affect cellular energy use and availability, oxidative stress management, muscle damage, central nervous system signaling metabolites, nutrients, and compound bioavailability, providing new insights into metabolic alterations associated with exercise and cardiorespiratory fitness levels in trained athletes.

5.
Mil Med ; 184(11-12): 875-881, 2019 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30941410

RESUMO

INTRODUCTION: Military personnel must remain physically active to meet operational requirements. Military physical training not only provides the performance capabilities required for performing occupational tasks but also fosters the development of sport. Thus, Armed Forces across the world have historically invested in developing elite- and Olympic-level athletes. This study aimed to assess the anthropometric and physiological differences among groups of Brazilian military athletes (MA), non-military athletes (A), and military non-athletes (M). MATERIALS AND METHODS: Seventy-five individuals participated in the study: 17 MA (23.7 ± 4.8 years), 27 A (24.7 ± 5.3 years), and 31 M (26.9 ± 3.3 years). MA and A individuals specialized in endurance sports, and had a mean weekly training volume of (100.0 ± 34.8 and 106.3 ± 40.5 Km; F = 0.894, p = 0.6), respectively. Anthropometric measures and maximal oxygen uptake (V̇O2máx) were assessed in all participants. Ergospirometry and anthropometry variables were analyzed with one-way analysis of variance (ANOVA) for independent measures. Comparisons of weekly training volume (km) and training experience (years) were performed only between the A and MA using the Student's t-test for independent samples. For a multidimensional approach, Partial least squares discriminant analysis (PLS-DA) was performed for all variables using the online tool MetaboAnalyst. RESULTS: We found no differences in anthropometric and physiological profiles between A and MA, but significant differences between M and MA/A in body mass index (kg/m2) (BMI), body fat percentage, fat mass (kg), waist circumference (cm) (WC), somatotype, and V̇O2máx (mL min-1 kg-1). CONCLUSION: In conclusion, military endurance athletes have similar anthropometric and physiological profiles to non-military athletes and superior levels to non-athlete military. These findings indicate that the Brazilian Armed Forces scouting system has been successful in identifying endurance athletic talent in line with their historic role of developing sport in Brazil.


Assuntos
Antropometria/métodos , Atletas/estatística & dados numéricos , Exercício Físico/fisiologia , Militares/estatística & dados numéricos , Aptidão Física/fisiologia , Adulto , Análise de Variância , Índice de Massa Corporal , Brasil , Feminino , Humanos , Masculino , Consumo de Oxigênio/fisiologia
6.
Toxins (Basel) ; 11(4)2019 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-30987210

RESUMO

We report for the first time the efficient use of accelerated solvent extraction (ASE) for extraction of ricin to analytical purposes, followed by the combined use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and MALDI-TOF MS/MS method. That has provided a fast and unambiguous method of ricin identification for in real cases of forensic investigation of suspected samples. Additionally, MALDI-TOF MS was applied to characterize the presence and the toxic activity of ricin in irradiated samples. Samples containing ricin were subjected to ASE, irradiated with different dosages of gamma radiation, and analyzed by MALDI-TOF MS/MS for verification of the intact protein signal. For identification purposes, samples were previously subjected to SDS-PAGE, for purification and separation of the chains, followed by digestion with trypsin, and analysis by MALDI-TOF MS/MS. The results were confirmed by verification of the amino acid sequences of some selected peptides by MALDI-TOF MS/MS. The samples residual toxic activity was evaluated through incubation with a DNA substrate, to simulate the attack by ricin, followed by MALDI-TOF MS/MS analyses.


Assuntos
Ricina/análise , Acetona/química , Sequência de Aminoácidos , Eletroforese em Gel de Poliacrilamida , Hexanos/química , Peptídeos/análise , Peptídeos/química , Ricina/química , Solventes/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrometria de Massas em Tandem
7.
Int J Sports Physiol Perform ; 14(6): 711-717, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-30427247

RESUMO

Purpose: Soccer is one of the most popular sports worldwide, a physical activity of great physiological demand and complexity. Currently, numerous trials involving physiological responses such as hypertrophy, energy expenditure, vasodilation, cardiac output, VO2max, and recovery have supported the possibility of genomic predictors' affecting performance. In a complementary way to association studies with single nucleotide polymorphisms (SNPs), the objective was to evaluate if the use of population genetics data from human-genomics databases can provide information for a better understanding of the relationship between heritability and sport performance. Methods: The study included 25 healthy male professional soccer players (25.5 [4.3] y, 177.4 [6.4] cm, 76.4 [6.4] kg, body fat 10.5% [4.3%]) from the Brazilian first-division soccer club. Anthropometric measurements and field and isokinetic tests were performed to evaluate performance and physiologic parameters of subjects. Moreover, 10 genetic polymorphisms previously related to performance were genotyped. The genotypes of the same polymorphisms were obtained for 2504 individuals from the populations deposited in the 1000 Genomes database. A principal-component analysis and matrix genetic-distances approach (Fst) were evaluated. Results: As expected, the admixture Brazilian population has numerous genetic similarities with the European and American populations from genomic databases. Although the African component is absolutely recognized in genomes from the Brazilian population, using the specific performance-related SNPs, surprisingly the African population was one of the most genetically distant of the players (P < .00001). Conclusions: The early results suggest a selective pressure on genes of elite soccer players, possibly related simultaneously to physical-performance, environmental, cognitive, and sociocultural aspects.


Assuntos
Atletas , Genética Populacional , Polimorfismo de Nucleotídeo Único , Futebol , Adulto , Antropometria , Desempenho Atlético , Brasil , Teste de Esforço , Genótipo , Humanos , Masculino , Dinamômetro de Força Muscular , Adulto Jovem
8.
PLoS One ; 13(3): e0194264, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29538445

RESUMO

Ethanol (ET) is a substance that modulates the Central Nervous System (CNS). Frequently, ET intake occurs combined with energy drinks, which contain taurine (TA), an important amino acid found in the body (i.e brain and muscles). Although TA administration has been used in the improvement of physical performance, the impact of TA, ET and exercise remains unknown. This study aimed to analyze the acute effect of 6g of Taurine (TA), 0.6 mL∙kg-1 of Ethanol (ET), and Taurine combined with Ethanol (TA+ET) ingestion on the electrocortical activity before and after a moderate intensity exercise in 9 subjects, 5 women (counterbalanced experimental design). In each of the 4 treatments (Placebo-PL, TA, ET and TA+ET), electroencephalography (EEG) tests were conducted in order to analyze changes in absolute beta power (ABP) in the frontal lobe in 3 moments: baseline (before ingestion), peak (before exercise) and post-exercise. In the PL treatment, the frontal areas showed decrease in ABP after exercise. However, in the ET+TA treatment, ABP values were greater after exercise, except for Fp1. The ET treatment had no effect on the Superior Frontal Gyrus area (F3, Fz and F4) and ABP decreased after exercise in Fp1 and Fp2. In the TA treatment, ABP increased after exercise, while it decreased at the peak moment in most of the frontal regions, except for Fp1, F3 and Fz. We concluded that after a moderate intensity exercise, a decrease in cortical activity occurs in placebo treatment. Moreover, we found a inhibitory effect of TA on cortical activity before exercise and a increased in cortical activity after exercise. A small ET dose is not enough to alter ABP in all regions of the frontal cortex and, in combination with TA, it showed an increase in the frontal cortex activity at the post-exercise moment.


Assuntos
Etanol/farmacologia , Exercício Físico/fisiologia , Lobo Frontal/efeitos dos fármacos , Lobo Frontal/fisiologia , Taurina/farmacologia , Feminino , Voluntários Saudáveis , Humanos , Masculino , Modelos Biológicos , Fatores de Tempo
9.
Neurosci Lett ; 657: 5-10, 2017 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-28743582

RESUMO

Taurine and alcohol has been popularly ingested through energy drinks. Reports from both compounds shows they are active on nervous system but little is known about the acute effect of these substances on the frontal cortex in an exercise approach. The aim of this study was to determine the effects of 0,6mldL-1 of ethanol (ET), 6g of taurine (TA), and taurine with ethanol (TA+ET) intake on absolute alpha power (AAP) in the frontal region, before and after exercise. Nine participants were recruited, five women (22±3years) and four men (26±5years), for a counterbalanced experimental design. For each treatment, the tests were performed considering three moments: "baseline", "peak" and "post-exercise". In the placebo treatment (PL), the frontal areas showed AAP decrease at the post-exercise. However, in the TA, AAP decreased at peak and increased at post-exercise. In the ET treatment, AAP increased at the peak moment for the left frontal electrodes. In the TA+ET treatment, an AAP increase was observed at peak, and it continued after exercise ended. These substances were able to produce electrocortical activity changes in the frontal regions after a short duration and low intensity exercise. Left and right regions showed different AAP dynamics during peak and post-exercise moments when treatments were compared.


Assuntos
Ritmo alfa/efeitos dos fármacos , Depressores do Sistema Nervoso Central/farmacologia , Etanol/farmacologia , Exercício Físico/fisiologia , Lobo Frontal/efeitos dos fármacos , Taurina/farmacologia , Adulto , Depressores do Sistema Nervoso Central/administração & dosagem , Etanol/administração & dosagem , Feminino , Humanos , Masculino , Taurina/administração & dosagem , Adulto Jovem
10.
Biol Sport ; 34(4): 353-359, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29472738

RESUMO

Taurine (TA) ingestion has been touted as blunting the deleterious effects of ethanol (ET) ingestion on motor performance. This study investigated the effects of ingestion of 0.6 mL·kg-1 of ET, 6 grams of TA, and ethanol in combination with taurine (ET+TA) on economy of movement (EM) and heart rate (HR). Nine volunteers, five female (22 ± 3 years) and four male (26 ± 5 years), participated in a study that used a counterbalanced experimental design. EM and HR were measured for 6 min while the subjects were pedalling at a fixed load 10% below the anaerobic threshold. The blood alcohol concentration (BAC) was similar between ET and ET+TA treatments at 30 min after ingestion and after exercise (12.3 mmol·L-1 vs. 13.7 mmol·L-1, and 9.7 mmol • L-1 vs 10.9 mmol·L-1, respectively). EM was significantly different among treatments, with lower mL·W-1 following ingestion of TA (-7.1%, p<0.001) than placebo and ET+TA (-2.45%, p=0.001) compared to ET. HR (bpm) was significantly (p<0.05) higher for ET (137 ± 14 bpm) than the other three treatments (placebo = 129 ± 14 bpm; TA = 127 ± 11 bpm; TA+ET = 133 ± 12 and ET = 137 ± 14 bpm). Taurine improved EM when compared to placebo or ET, and reduced HR when compared to ET. The combination of ET+TA also enhanced EM compared to placebo, and reduced HR in comparison to ET alone. Therefore, these findings indicate that taurine improves EM and counteracts ethanol-induced increases in HR during submaximal exercise.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...