Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats.

Kisspeptin (Kp-10) is a neuropeptide that binds to GPR54 receptors, exerting several functions mainly in the nervous and reproductive systems of the body. However, its effects and mechanisms of action on the skeletal system remain poorly understood. This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synthesis, percentage of area covered by MSCs/field and mineralized nodules/field, and immunocytochemistry of the GPR54 receptor tests. Furthermore, evaluation of gene transcripts for type I collagen, Runx-2, Bmp-2, bone sialoprotein, osteocalcin and osteopontin was performed using real-time RT-qPCR. It was observed that MSCs expressed GPR54 receptor to which Kp-10 binds during osteogenic differentiation, promoting a negative effect on osteogenic differentiation. This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2. Thus, Kp-10 inhibits in vitro osteogenic differentiation of MSCs extracted from the BM of adult Wistar rats.

Shiatsu-associated physical therapy on pain and fatigue on people with multiple sclerosis / Shiatsu associado à fisioterapia na dor e fadiga em pessoas com esclerose múltipla

Abstract Introduction: Pain and fatigue are common symptoms in multiple sclerosis (MS). Shiatsu, a technique that uses the pressure of fingers, associated with manual therapy, exercises and stretching can be used to control these symptoms. Objective: To evaluate the effect of Shiatsu associated with physical therapy on pain and fatigue on people with MS. Methods: Randomized clinical trial with people diagnosed with MS divided into two groups: intervention group (IG) - Shiatsu-associated physical therapy (n = 9), and control group (CG, n = 8). Participants were assessed before and after treatment by the Expanded Disability Status Scale (EDSS), Neuropathic Pain Questionnaire (DN4), Visual Analog Scale (VAS) and Fatigue Impact Scale (MFIS), and description of sociodemographic. Results: Seventeen people with MS (9 men) aged 45.18 ± 3.06 years participated in this study. In the total sample, the average of DN4 was 1.65 ± 20.02, VAS was 2.29 ± 2.80, MFIS was 39.47 ± 29.67, and 52.9 % had a score > 38 in the MFIS that corresponds to presence of fatigue. The values pre/post-intervention/grade of p, respectively in the IG were DN4: 2.78 ± 2.16/2.0 ± 2.12/0.432, EVA: 3.22 ± 3.27/0.33 ± 1.00/0.023, total MFIS: 44.44 ± 35.91/35 ± 31.70/0.068. In the CG the values were DN4 : 0.38 ± 0.744/2.25 ± 2.71/0.054, EVA : 1.25 ± 1.83/3.63 ± 2.38/0.043, and MFIS : 33.88 ± 21.68/25.13 ± 24.22/0.379. Conclusion: Shiatsu associated with Physiotherapy was effective in improving pain and fatigue in individuals with MS.

Resumo Introdução: A dor e a fadiga são sintomas comuns na esclerose múltipla (EM). O Shiatsu, técnica que utiliza a pressão dos dedos, associado à terapia manual, exercícios e alongamentos pode ser utilizado para controlar esses sintomas. Objetivo: Avaliar o efeito do Shiatsu associado à fisioterapia na dor e fadiga em pessoas com EM. Métodos: Ensaio clínico randomizado com pessoas com diagnóstico de EM, divididas aleatoriamente em grupo intervenção (GI), que recebeu fisioterapia associada ao Shiatsu (n = 9), e grupo controle (GC, n = 8). Os participantes foram avaliados antes e após o tratamento pela Escala Expandida do Estado da Incapacidade (EDSS), questionário para dor neuropática (DN4), Escala Visual de Dor (EVA) e Escala de Impacto da Fadiga (MFIS), edescrição dos dados sociodemográficos. Resultados: Participaram deste estudo 17 pessoas com EM (9 homens), com idade média de 45,18 ± 3,06 anos. Na amostra total, a média do DN4 foi de 1,65 ± 20,02, da EVA foi de 2,29 ± 2,80, MFIS foi de 39,47 ± 29,67 e 52,9% apresentaram escore > 38 no MFIS, que corresponde à presença de fadiga. Os valores no pré/pós-intervenção/valor de p no GI foram, respectivamente, DN4: 2,78 ± 2,16/2,0 ± 2,12/0,432, EVA: 3,22 ± 3,27/0,33 ± 1,00/0,023, MFIS: 44,44 ± 35,91/35 ± 31,70/0,068. No GC foram DN4: 0,38 ± 0,744/2,25 ± 2,71/ 0,054, EVA: 1,25 ± 1,83/3,63 ± 2,38/0,043 e MFIS: 33,88 ± 21,68/25,13 ± 24,22/0,379. Conclusão: O Shiatsu associado à fisioterapia foi eficaz na melhora da dor e fadiga em indivíduos com EM.

Quality of life in patients with oculocutaneous albinism.

BACKGROUND: The social reality of the albino needs to be more studied in Brazil, as myths and social segregation regarding this illness are likely to be found in the country, with psychosocial and medical implications. OBJECTIVE: As this subject has not been referenced in previous scientific articles in Brazil, this research intends to evaluate the quality of life of the albinos that treated at our medical institution. METHODS: The quality of life was evaluated through the WHOQOL-BREF. Furthermore, two aspects of main relevance in the lives of the albinos were also objects of research, low vision and skin cancer. The sample consisted of forty oculocutaneous albinos and a control group of forty healthy individuals, matched by sex and age. RESULTS: Among the participants, 57.7% were between 18 and 40 years old, 28.2% were between 41 and 60, and 14.1% were over 60. 42.1% had skin cancer before the study, 18.4% had skin cancer during the study and 89.5% stated visual deficit. The results obtained in the questionnaires showed a statistically significant difference in the physical domain, with P < 0.001. CONCLUSION: Low vision combined with skin lesions and social stigma may contribute to disturbances in the quality of life of oculocutaneous albinos. The results presented in this study demonstrated the vulnerability of the affected individuals and the special care required by those patients, at the same time that the need for further research is highlighted in order to better elucidate the aspects related to albinism.

Quality of life in patients with oculocutaneous albinism

AbstractBACKGROUND:The social reality of the albino needs to be more studied in Brazil, as myths and social segregation regarding this illness are likely to be found in the country, with psychosocial and medical implications.OBJECTIVE:As this subject has not been referenced in previous scientific articles in Brazil, this research intends to evaluate the quality of life of the albinos that treated at our medical institution.METHODS:The quality of life was evaluated through the WHOQOL-BREF. Furthermore, two aspects of main relevance in the lives of the albinos were also objects of research, low vision and skin cancer. The sample consisted of forty oculocutaneous albinos and a control group of forty healthy individuals, matched by sex and age.RESULTS:Among the participants, 57.7% were between 18 and 40 years old, 28.2% were between 41 and 60, and 14.1% were over 60. 42.1% had skin cancer before the study, 18.4% had skin cancer during the study and 89.5% stated visual deficit. The results obtained in the questionnaires showed a statistically significant difference in the physical domain, with P < 0.001.CONCLUSION:Low vision combined with skin lesions and social stigma may contribute to disturbances in the quality of life of oculocutaneous albinos. The results presented in this study demonstrated the vulnerability of the affected individuals and the special care required by those patients, at the same time that the need for further research is highlighted in order to better elucidate the aspects related to albinism.