Molecular epidemiology identifies the expansion of the DENV2 epidemic lineage from the French Caribbean Islands to French Guiana and mainland France, 2023 to 2024.

In 2023, dengue virus serotype 2 (DENV2) affected most French overseas territories. In the French Caribbean Islands, viral circulation continues with > 30,000 suspected infections by March 2024. Genome sequence analysis reveals that the epidemic lineage in the French Caribbean islands has also become established in French Guiana but not Réunion. It has moreover seeded autochthonous circulation events in mainland France. To guide prevention of further inter-territorial spread and DENV introduction in non-endemic settings, continued molecular surveillance and mosquito control are essential.

New records of Neopetrosia carbonaria (Lamarck, 1814) from the Brazilian coast reveal new morphological features and spicule types.

Sponges of the family Petrosiidae have usually a stony and brittle texture due the high silica content of the mineral skeleton that is formed by a more-or-less regular isotropic reticulation of undefined primary and secondary tracts. Neopetrosia species are distributed worldwide and twelve species are found in the Tropical Western Atlantic Ocean, of which four are recorded from the Brazilian coast. Here, we describe new Neopetrosia carbonaria specimens from several localities along the NE Brazilian coast, analyze the Neopetrosia carbonaria holotype and review the previous record of N. carbonaria from the Brazilian coast. Our study reveals new morphological features and spicule types in the Brazilian populations of N. carbonaria. Now, Neopetrosia carbonaria is defined by a thick encrusting to repent or ramose sponge, with reddish brown to brown color or dark green to black color in vivo, two categories of oxeas as megascleres and raphidiform toxas as microscleres. These toxas are quite rare and occur in both black and brown sponges. Due the absence and rarity of raphidiform toxas in some specimens, unrelated to its color, we assume that these differences are intraspecific. However, we suggest that all records of Neopetrosia carbonaria should be reevaluated, since toxas can be easily overlooked, added to the use of molecular methods to investigate the relationship between the Caribbean and Brazilian populations.

Tissue repair capacity of bioceramic endodontic sealers in rat subcutaneous tissue.

This study aimed to evaluate the tissue repair capacity of four bioceramic endodontic sealers by quantifying type I and III collagen fibers. The following sealers were tested: EndoSequence BC Sealer (Brasseler, Brasseler, Savannah, USA), Bio C Sealer (Angelus, Londrina, Brazil), Bioroot RCS (Septodont, Santa Catarina, Brazil), and Sealer Plus BC (MKLife, Porto Alegre, Brazil). Polyethylene tubes 1.5 mm in diameter and 1 cm in length containing the endodontic sealers were implanted in the subcutaneous tissue of five rats (Rattus norvegicus albinus, Wistar lineage). After 14 days, the animals were euthanized, and collagen fibers were quantified from the histological tissue sections. Given a non-normal distribution of the data, a gamma regression with log link function was employed and implemented through the generalized linear models module, was used to test whether there was a significant difference between the sealers. The pairwise comparison was performed using Least significant difference. There were significant differences between the sealers for type I (p=0.001), type III (p=0.023), and total collagen (p=0.002). Overall, Bioroot sealer was statistically superior to the other sealers, except in the analysis of type III collagen, in which there was no difference between the Bioroot sealer and Bio C Sealer sealer and the control group (p>0.05). Bioroot RCS bioceramic endodontic sealer stimulates a greater production of collagen.

A design process to define public challenges addressing SDGs / Un proceso de diseño para definir los desafíos públicos relacionados con los ODS / Um processo de design para definir desafios públicos envolvendo os ODS

Abstract How might we define local innovation challenges that are user-centered but also address strategic goals, such as the Sustainable Development Goals (SDGs)? We propose a design process that introduces a relevant contribution to the practices of the Mission-oriented Innovation Policies (MIPs) field as it goes from Grand Societal Challenges (GSCs) to local innovation challenges, which are more relevant to local needs and capabilities of solution. The main goal of the design process is to go from a GSC, such as "access to health" and one of its missions, like "reducing chronic Non-Communicable Diseases (NCD)," to a more able-to-solve innovation challenge, passing by its mission, and different problems and aspects. Snowball sampling methods allow us to find and mobilize electable subjects and design thinking techniques to process problem mapping, election, and clustering, as well as create challenge statements. It was possible to translate GSC to the local reality while reducing contestation and complexity and producing a problem-grouped map with prioritization and three problem statements (challenges) with open phrasing and solution criteria to foster the users' desired outcomes.

Resumen ¿Cómo podemos definir desafíos locales de innovación local que estén centrados en el usuario pero que también aborden objetivos estratégicos, como los Objetivos de Desarrollo Sostenible (ODS)? Proponemos un proceso de diseño que introduce una contribución relevante a las prácticas en el campo de las políticas de innovación orientadas por misión (en inglés, Mission-oriented Innovation Policies - MIPs), ya que va desde los grandes desafíos sociales (en inglés, Grand Societal Challenges - GSCs), comenzando con los ODS, hasta los desafíos locales de innovación, que son más relevantes para necesidades locales y capacidades de solución. El objetivo principal del proceso de diseño es ir desde un GSC, como "acceso a la salud" y una de sus misiones, como "reducir las Enfermedades Crónicas No Transmisibles (ECNT)", a un desafío de innovación más concreto, pasando por la misión, y por diferentes problemas y aspectos. Los métodos de muestreo de bola de nieve nos permiten encontrar y movilizar sujetos elegibles, y técnicas de design thinking, procesar el mapeo, la elección y la agrupación de problemas; y crear declaraciones de desafío. Fue posible traducir GSC a la realidad local, al mismo tiempo que se reducía la contestación y la complejidad, y se producía un plano agrupado de problemas con priorización, y tres declaraciones de problemas (desafíos) con redacción abierta y criterios de solución para promover los resultados deseados por los usuarios.

Resumo Como podemos definir os desafios locais de inovação que são centrados no usuário, mas também abordam objetivos estratégicos, como os Objetivos de Desenvolvimento Sustentável (ODS)? Propomos um processo de design que apresenta uma contribuição relevante para as práticas no campo das políticas de inovação orientadas para a missão (em inglês, Mission-oriented Innovation Policies - MIPs), uma vez que vai dos grandes desafios societais (em inglês, Grand Societal Challenges - GSCs) aos desafios locais de inovação, que são mais relevantes para as necessidades locais e capacidades de solução. O principal objetivo do processo de desenho é passar de um GSC, como o "acesso à saúde" e uma de suas missões, como "reduzir as Doenças Crônicas Não Transmissíveis (DCNT)", para um desafio de inovação mais capaz de resolver, passando pela sua missão, diferentes problemas e aspectos. Os métodos de amostragem bola de neve nos permitem encontrar e mobilizar assuntos elegíveis e técnicas de design thinking para processar o mapeamento de problemas, eleição e agrupamento; e criar declarações de desafio. Foi possível traduzir o GSC para a realidade local, reduzindo a contestação e a complexidade, e produzindo um mapa agrupado de problemas com priorização e três declarações de problemas (desafios) com formulação aberta e critérios de solução para promover os resultados desejados pelos usuários.

Tissue repair capacity of bioceramic endodontic sealers in rat subcutaneous tissue

Abstract This study aimed to evaluate the tissue repair capacity of four bioceramic endodontic sealers by quantifying type I and III collagen fibers. The following sealers were tested: EndoSequence BC Sealer (Brasseler, Brasseler, Savannah, USA), Bio C Sealer (Angelus, Londrina, Brazil), Bioroot RCS (Septodont, Santa Catarina, Brazil), and Sealer Plus BC (MKLife, Porto Alegre, Brazil). Polyethylene tubes 1.5 mm in diameter and 1 cm in length containing the endodontic sealers were implanted in the subcutaneous tissue of five rats (Rattus norvegicus albinus, Wistar lineage). After 14 days, the animals were euthanized, and collagen fibers were quantified from the histological tissue sections. Given a non-normal distribution of the data, a gamma regression with log link function was employed and implemented through the generalized linear models module, was used to test whether there was a significant difference between the sealers. The pairwise comparison was performed using Least significant difference. There were significant differences between the sealers for type I (p=0.001), type III (p=0.023), and total collagen (p=0.002). Overall, Bioroot sealer was statistically superior to the other sealers, except in the analysis of type III collagen, in which there was no difference between the Bioroot sealer and Bio C Sealer sealer and the control group (p>0.05). Bioroot RCS bioceramic endodontic sealer stimulates a greater production of collagen.

Resumo Este estudo visou avaliar a capacidade de reparação de tecidos de quatro cimentos endodônticos biocerâmicos através da quantificação de fibras colágenas de tipo I e III. Foram testados os seguintes cimentos: EndoSequence BC Sealer (Brasseler, Brasseler, Savannah, EUA), Bio C Sealer (Angelus, Londrina, Brasil), Bioroot RCS (Septodont, Santa Catarina, Brasil), e Sealer Plus BC (MKLife, Porto Alegre, Brasil). Foram implantados tubos de polietileno de 1,5 mm de diâmetro e 1 cm de comprimento contendo os cimentos endodônticos no tecido subcutâneo de cinco ratos (Rattus norvegicus albinus, linhagem Wistar). Após 14 dias, os animais foram eutanasiados e as fibras colágenas foram quantificadas a partir de cortes histológicos do tecido. Diante de uma distribuição não-normal dos dados, uma regressão gama com função de ligação log, implementada por meio do módulo de modelos lineares generalizados, foi empregada para testar se havia diferença significativa entre os cimentos. A comparação dois a dois foi realizada utilizando Least significant difference. Houve diferença significativa entre os cimentos para os colágenos tipo I (p=0,001), tipo III (p=0,023) e colágeno total (p=0,002). No geral, o cimento Bioroot foi estatisticamente superior aos demais cimentos, com exceção na análise do colágeno tipo III na qual não houve diferença entre o cimento Bioroot e o cimento Bio C Sealer e o grupo controle (p>0,05). O cimento endodôntico biocerâmico Bioroot RCS foi capaz de estimular uma maior produção de colágeno.

Management technology for implementing the Systematization of Nursing Care.

OBJECTIVE: To describe the construction of a management technology aimed at implementing the Systematization of Nursing Care in nursing services. METHOD: This is a methodological, qualitative and explanatory study, based on the normative and legal framework of COFEN Resolution 358/2009. It comprised the theoretical construction of instruments for practice anchored in the literature and expertise of a group of 40 nurses, between April 2020 and June 2021. RESULTS: The technology is outlined from the dimensions of method, personnel and nursing instruments that support the Systematization of Nursing Care tripod. It consists of an explanatory model of an operational management matrix and a checklist-type instrument for follow-up/monitoring of Systematization of Nursing Care management in services. CONCLUSION: Management technology is inserted as a solution to improve organizational performance, health care, clinical decision support, planning, administration, organization of services and professional practice, and create favorable conditions for applying the Process of Nursing at its fullest.

New Pregnane Glycosides from Mandevilla dardanoi and Their Anti-Inflammatory Activity.

Mandevilla Lindl. is an important genus of the Apocynaceae family, not only as ornamental plants but also for its medicinal uses. In Brazil, Mandevilla species are indicated to treat asthma and skin infections, their anti-inflammatory potential and wound healing properties are also reported in the literature. Concerning their chemical composition, this group of plants is a conspicuous producer of pregnane glycosides. Mandevilla dardanoi is an endemic species from the Brazilian semiarid region not studied by any phytochemical methods. In view of the medicinal potential of Mandevilla species, this study aimed to isolate new pregnane glycosides from M. dardanoi. To achieve this main goal, modern chromatography techniques were employed. Five new pregnane glycosides, dardanols A-E, were isolated from the roots of M. dardanoi by HPLC. Their structures were determined using extensive 1D and 2D-NMR and mass spectrometry (MSn and HRESIMS) data. The cytotoxicity and the anti-inflammatory potential of these compounds were evaluated. The first was evaluated by measuring proinflammatory cytokines and nitric oxide production by stimulated macrophages. Dardanols were able to inhibit the production of nitric oxide and reduce IL-1ß and TNF-α. The current work demonstrates the chemodiversity of Brazilian semiarid species and contributes to amplifying knowledge about the biological potential of the Mandevilla genus.

Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil.

During these past years, several studies have provided serological evidence regarding the circulation of West Nile virus (WNV) in Brazil. Despite some reports, much is still unknown regarding the genomic diversity and transmission dynamics of this virus in the country. Recently, genomic monitoring activities in horses revealed the circulation of WNV in several Brazilian regions. These findings on the paucity of genomic data reinforce the need for prompt investigation of WNV infection in horses, which may precede human cases of encephalitis in Brazil. Thus, in this study, we retrospectively screened 54 suspicious WNV samples collected between 2017 and 2020 from the spinal cord and brain of horses with encephalitis and generated three new WNV genomes from the Ceará and Bahia states, located in the northeastern region of Brazil. The Bayesian reconstruction revealed that at least two independent introduction events occurred in Brazil. The first introduction event appears to be likely related to the North American outbreak, and was estimated to have occurred in March 2013.The second introduction event appears to have occurred in September 2017 and appears to be likely related to the South American outbreak. Together, our results reinforce the importance of increasing the priority of WNV genomic monitoring in equines with encephalitis in order to track the dispersion of this emerging pathogen through the country.

Systematization of Nursing Care: seeking defining and differentiating theoretical contours.

The objective of this study was to elaborate theoretical propositions to help understanding the Systematization of Nursing Care as a distinct phenomenon of the Nursing Process. It is a reflective, theoretical study that presented two categories: "Systematization of Nursing Care: seeking differentiating contours" and "Systematization of Nursing Care: seeking defining contours". It was identified that the systematization is not concerned with guiding the professional nursing care itself, since this issue, together with the guidelines regarding the elaboration of clinical documentation resulting from the implemented nursing care, is related to the process. Despite what the Systematization of Nursing Care is, the idea is that it is a field of knowledge that, through its three pillars and constituent elements, supports the structuring of nursing services and, consequently, the organization or reorganization of professional work in nursing. It is concluded that systematization, in the light of the theoretical propositions presented here, is an area of knowledge that represents a particular way of carrying out the management of nursing services when the three pillars that compose it are considered.

Systematization of Nursing Care: seeking defining and differentiating theoretical contours / Sistematización de la Asistencia de Enfermería (SAE): buscando contornos teóricos definitorios y distintos / Sistematização da Assistência de Enfermagem: buscando contornos teóricos definitórios e diferenciadores

ABSTRACT The objective of this study was to elaborate theoretical propositions to help understanding the Systematization of Nursing Care as a distinct phenomenon of the Nursing Process. It is a reflective, theoretical study that presented two categories: "Systematization of Nursing Care: seeking differentiating contours" and "Systematization of Nursing Care: seeking defining contours". It was identified that the systematization is not concerned with guiding the professional nursing care itself, since this issue, together with the guidelines regarding the elaboration of clinical documentation resulting from the implemented nursing care, is related to the process. Despite what the Systematization of Nursing Care is, the idea is that it is a field of knowledge that, through its three pillars and constituent elements, supports the structuring of nursing services and, consequently, the organization or reorganization of professional work in nursing. It is concluded that systematization, in the light of the theoretical propositions presented here, is an area of knowledge that represents a particular way of carrying out the management of nursing services when the three pillars that compose it are considered.

RESUMEN Se propuso elaborar proposiciones teóricas que auxilien en la comprensión de la Sistematización de la Asistencia de Enfermería como un fenómeno distinto del Proceso de Enfermería. Estudio teórico reflexivo que presentó dos clases: "Sistematización de la Asistencia de Enfermería: buscando contornos diferenciadores" y "Sistematización de la Asistencia de Enfermería: buscando contornos definitorios". Se identificó que la sistematización no se ocupa en orientar la asistencia profesional de enfermería por si misma, una vez que esa cuestión, sumada a las orientaciones a respecto de la elaboración de la documentación clínica resultante de la asistencia de enfermería implementada, se refiere al proceso. A pesar de lo que es la Sistematización de la Asistencia de Enfermería, se conviene definir como un campo de conocimiento que, por medio de sus tres pilares y elementos que la constituyen, soporta la estructuración de servicios de enfermería y, por así decirlo, la organización o reorganización del trabajo profesional en enfermería. Se concluye que la sistematización, siguiendo las proposiciones teóricas presentadas, constituye área de conocimiento que representa una forma particular de realizar la gestión de servicios de enfermería cuando se consideran los tres pilares que la componen.

RESUMO Objetivou-se elaborar proposições teóricas que auxiliem na compreensão da Sistematização da Assistência de Enfermagem enquanto fenômeno distinto do Processo de Enfermagem. Estudo teórico reflexivo que apresentou duas categorias: "Sistematização da Assistência de Enfermagem: buscando contornos diferenciadores" e "Sistematização da Assistência de Enfermagem: buscando contornos definitórios". Identificou-se que a sistematização não se ocupa em orientar a assistência profissional de enfermagem em si, já que essa questão, aliada às orientações quanto à elaboração da documentação clínica resultante da assistência de enfermagem implementada, diz respeito ao processo. A despeito do que é a Sistematização da Assistência de Enfermagem, tem-se que se trata de um campo de conhecimento que, por meio de seus três pilares e elementos constituintes, suporta a estruturação de serviços de enfermagem e, por consequência, a organização ou reorganização do trabalho profissional em enfermagem. Conclui-se que a sistematização, à luz das proposições teóricas aqui apresentadas, constitui área de conhecimento que representa uma forma particular de realizar a gestão de serviços de enfermagem quando se consideram os três pilares que a compõem.

Management technology for implementing the Systematization of Nursing Care / Tecnología de gestión para la implementación de la Sistematización de la Atención de Enfermería / Tecnologia gerencial para implementação da Sistematização da Assistência de Enfermagem

ABSTRACT Objective: To describe the construction of a management technology aimed at implementing the Systematization of Nursing Care in nursing services. Method: This is a methodological, qualitative and explanatory study, based on the normative and legal framework of COFEN Resolution 358/2009. It comprised the theoretical construction of instruments for practice anchored in the literature and expertise of a group of 40 nurses, between April 2020 and June 2021. Results: The technology is outlined from the dimensions of method, personnel and nursing instruments that support the Systematization of Nursing Care tripod. It consists of an explanatory model of an operational management matrix and a checklist-type instrument for follow-up/monitoring of Systematization of Nursing Care management in services. Conclusion: Management technology is inserted as a solution to improve organizational performance, health care, clinical decision support, planning, administration, organization of services and professional practice, and create favorable conditions for applying the Process of Nursing at its fullest.

RESUMEN Objetivo: Describir la construcción de una tecnología de gestión dirigida a implementar la Sistematización de la Atención de Enfermería en los servicios de enfermería. Método: Estudio metodológico, cualitativo y explicativo, sustentado en el marco normativo y legal de la Resolución COFEN nº 358/2009. Comprendió la construcción teórica de instrumentos para la práctica anclados en la literatura y la experiencia de un grupo de 40 enfermeros, entre abril de 2020 y junio de 2021. Resultados: La tecnología se perfila a partir de las dimensiones de método, personal e instrumentos de enfermería que sustentan el trípode de la Sistematización de la Atención de Enfermería. Consiste en un modelo explicativo de matriz de gestión operativa y un instrumento tipo checklist para el seguimiento/monitoreo de la gestión de la Sistematización de la Atención de Enfermería en los servicios. Conclusión: La tecnología gerencial se inserta como una solución para mejorar el desempeño organizacional, el cuidado de la salud, el apoyo a la decisión clínica, la planificación, la administración, la organización de los servicios y la práctica profesional, y crear condiciones favorables para la aplicación del Proceso de Enfermería en su plenitud.

RESUMO Objetivo: Descrever a construção de uma tecnologia gerencial voltada à implementação da Sistematização da Assistência de Enfermagem em serviços de enfermagem. Método: Estudo metodológico, qualitativo e explicativo, sustentado no referencial normativo e legal da Resolução COFEN nº 358/2009. Compreendeu a construção teórica de instrumentos para a prática ancorados na literatura e na expertise de um grupo de 40 enfermeiros, entre os meses de abril de 2020 e junho de 2021. Resultados: A tecnologia está delineada a partir das dimensões método, pessoal e instrumentos de enfermagem que sustentam o tripé da Sistematização da Assistência de Enfermagem. É constituída por um modelo explicativo de uma matriz de gestão operacional e de um instrumento do tipo checklist para o acompanhamento/monitoramento da gestão da Sistematização da Assistência de Enfermagem nos serviços. Conclusão A tecnologia gerencial se insere como uma solução para melhorar a performance organizacional, a atenção à saúde, o apoio às decisões clínicas, o planejamento, a administração, a organização dos serviços e a prática profissional, e criar condições favoráveis para a aplicação do Processo de Enfermagem em sua plenitude.

MEWAR: Development of a Cross-Platform Mobile Application and Web Dashboard System for Real-Time Mosquito Surveillance in Northeast Brazil.

Mosquito surveillance is a crucial process for understanding the population dynamics of mosquitoes, as well as implementing interventional programs for controlling and preventing the spread of mosquito-borne diseases. Environmental surveillance agents who performing routine entomological surveys at properties in areas where mosquito-borne diseases are endemic play a critical role in vector surveillance by searching and destroying mosquito hotspots as well as collate information on locations with increased infestation. Currently, the process of recording information on paper-based forms is time-consuming and painstaking due to manual effort. The introduction of mobile surveillance applications will therefore improve the process of data collection, timely reporting, and field worker performance. Digital-based surveillance is critical in reporting real-time data; indeed, the real-time capture of data with phones could be used for predictive analytical models to predict mosquito population dynamics, enabling early warning detection of hotspots and thus alerting fieldworker agents into immediate action. This paper describes the development of a cross-platform digital system for improving mosquito surveillance in Brazil. It comprises of two components: a dashboard for managers and a mobile application for health agents. The former enables managers to assign properties to health workers who then survey them for mosquitoes and to monitor the progress of inspection visits in real-time. The latter, which is primarily designed as a data collection tool, enables the environmental surveillance agents to act on their assigned tasks of recording the details of the properties at inspections by filling out digital forms built into the mobile application, as well as details relating to mosquito infestation. The system presented in this paper was co-developed with significant input with environmental agents in two Brazilian cities where it is currently being piloted.

Implications of Nursing Care Systematization in Brazilian professional practice. / Implicações da Sistematização da Assistência de Enfermagem na prática profissional brasileira.

To analyze the implications of the Nursing Care Systematization in Brazilian professional practice. Theoretical and reflective study, which listed as analytical categories the regulatory framework of systematization, the implications for professional practice, the limits and possibilities. In the legal frameworks, an evolution of understanding about Systematization was identified, which resulted in an update in the resolution that deals with the theme. It is emphasized that the implications for professional practice can be elucidated when the concepts, method, personnel and instruments are better understood, the three pillars of systematization, since they do not have conceptual and operational definitions in the current resolution. The lack of conceptual clarity about the term, as well as the lack of conceptual and operational definitions of its pillars and constituent elements, have made the understanding of the Nursing Care Systematization difficult, impacting the perception of its contribution to professional practice, since it is not rarely used as a synonym for the Nursing Process. The production of knowledge about Systematization must consider its limits and possibilities. Thus, contributing to the demarcation of the production of knowledge that is representative of this epistemological object.

Analisar as implicações da Sistematização da Assistência de Enfermagem na prática profissional brasileira. Estudo teórico e de caráter reflexivo, que elencou como categorias analíticas o marco regulador da sistematização, as implicações na prática profissional, os limites e as possibilidades. Identificou-se nos marcos legais uma evolução da compreensão sobre a Sistematização, o que resultou em atualização na resolução que trata da temática. Ressalta-se que as implicações na prática profissional podem ser elucidadas quando forem melhor compreendidos os conceitos de método, pessoal e instrumentos, os três pilares da Sistematização, uma vez que eles não possuem definições conceituais e operacionais na atual resolução. A falta de clareza conceitual acerca do termo, bem como a escassez de definições conceituais e operacionais de seus pilares e de elementos constituintes, têm dificultado a compreensão da Sistematização da Assistência de Enfermagem, impactando na percepção de sua contribuição à prática profissional, visto que não raramente é utilizada como sinônimo de Processo de Enfermagem. A produção de conhecimento acerca da Sistematização deve considerar seus limites e possibilidades. Dessa forma, contribuindo para a demarcação da produção de conhecimento que seja representativa desse objeto epistemológico.

El objetivo es analizar las implicaciones de la Sistematización de la Atención de Enfermería en la práctica profesional brasileña. Se trata de un estudio teórico y reflexivo que enumeró como categorías analíticas, el marco normativo de la sistematización, las implicaciones en la práctica profesional, sus límites y posibilidades. Se identificó una evolución de la comprensión sobre la Sistematización en los marcos legales, trayendo consigo una actualización de la resolución que trata sobre el tema. Cabe destacar que las implicaciones en la práctica profesional pueden dilucidarse cuando se entienden mejor los conceptos del método, del personal y de los instrumentos, los tres pilares de la Sistematización, ya que no existen definiciones conceptuales y operativas en la resolución actual. La falta de claridad conceptual sobre el término, así como la escasez de definiciones conceptuales y operativas de sus pilares y elementos constituyentes, han dificultado la comprensión de la Sistematización de la Atención de Enfermería, impactando en la percepción de su aporte a la práctica profesional, ya que muchas veces se utiliza como sinónimo de Proceso de Enfermería. La producción de conocimiento sobre la Sistematización debe considerar sus límites y posibilidades, contribuyendo, así, a la demarcación de la producción de conocimiento que represente a este objeto epistemológico.

Bone regeneration using Wollastonite/ß-TCP scaffolds implants in critical bone defect in rat calvaria.

In order to provide favorable conditions for bone regeneration, a lot of biomaterials have been developed and evaluated, worldwide. Composite biomaterials have gained notoriety, as they combine desirable properties of each isolated material. Thus, in this research, bone repair capacity of three developed formulations of ceramic scaffolds were evaluated histomorphometrically, after implantation. Scaffolds were based on wollastonite (W) andß-tricalcium phosphate (ß-TCP) composites in three different ratios (wt.%). ThirtyWistarrats were randomly assigned to three experimental groups: W-20 (20 W/80ß-TCP wt.%), W-60 (60 W/40ß-TCP wt.%), and W-80 (80 W/20ß-TCP wt.%), evaluated by optical microscopy at biological tests after 15 and 45 days of implantation. Throughout the study, the histological results evidenced that the scaffolds remained at the implantation site, were biocompatible and presented osteogenic potential. The percentage of neoformed mineralized tissue was more evident in the W-20 group (51%), at 45 days. The composite of the W-80 group showed more evident biodegradation than the biomaterials of the W-20 and W-60 groups. Thus, it is concluded that the scaffold containing 20 W/80ß-TCP (wt.%) promoted more evident bone formation, but all composites evaluated in this study showed notorious bioactivity and promising characteristics for clinical application.

Sistematização da Assistência de Enfermagem: compreensão à luz de seus pilares e elementos constituintes / Systematization of nursing care: understanding in the light of its pillars and constituent elements

Objetivo: Analisar os pilares da Sistematização da Assistência de Enfermagem e seus elementos constituintes. Método: Estudo teórico-reflexivo, que estabeleceu como categorias analíticas Método, Pessoal, Instrumentos. Resultados: Se decompôs cada pilar estruturante da Sistematização, o que permitiu identificar seus elementos constituintes, e ampliou-se a compreensão do que é Sistematização da Assistência de Enfermagem, considerando-a como fenômeno distinto do Processo de Enfermagem. Conclusão: Descortinaram-se os elementos que compõem cada pilar da Sistematização da Assistência de Enfermagem, permitindo melhor entendimento quando se discute a organização do trabalho profissional a partir desta, bem como, ampliou-se a compreensão do termo, conceitual e operacionalmente. (AU)

Objective: To analyze the pillars of the Nursing Care Systematization and its constituent elements. Method: Theoretical-reflective study, which established as analytical categories Method, Personnel, Instruments. Results: Each structuring pillar of the Systematization was decomposed, which allowed the identification of its constituent elements, and the understanding of what is Systematization of Nursing Care was expanded, considering it as a distinct phenomenon of the Nursing Process. Conclusion: The elements that make up each pillar of the Systematization of Nursing Assistance were revealed, allowing a better understanding when discussing the organization of professional work from it, as well as the understanding of the term, conceptually and operationally, was expanded. (AU)

Objetivo: Analizar los pilares de la Sistematización del Cuidado de Enfermería y sus elementos constitutivos. Método: Estudio teórico-reflexivo, que establece como categorías analíticas Método, Personal, Instrumentos. Resultados: se descompuso cada pilar estructurador de la Sistematización, lo que permitió la identificación de sus elementos constitutivos, y se amplió la comprensión de lo que es la Sistematización de la Atención de Enfermería, considerándolo como un fenómeno distinto del Proceso de Enfermería. Conclusión: se revelaron los elementos que componen cada pilar de la Sistematización de la Asistencia de Enfermería, lo que permitió una mejor comprensión al analizar la organización del trabajo profesional a partir de ella, así como la comprensión del término, conceptual y operativamente, se amplió. (AU)

Electrocorticographic description of the effects of anticonvulsant drugs used to treat lidocaine-induced seizures.

INTRODUCTION: Local anesthetics are widely used in clinical practice. While toxicity is rare, these drugs can cause potentially lethal seizures. OBJECTIVE: In the present study, we investigated the electrocorticographic (ECoG) and electromyographic patterns of seizures induced by acute lidocaine (LA) toxicity and treated with anticonvulsant drugs. The study used adult male Wistar rats to describe of the seizure-related behavior of LA and investigated the treatment with anticonvulsant drugs. RESULTS: The use of LA resulted in clear changes in the ECoG pattern, which presented characteristics of Status epilepticus, with increased intensity in all brainwaves. The decomposition of the cerebral waves showed an increase in the beta and gamma waves that may be related to tonic-clonic seizure. Although the treatment with anticonvulsants drugs reduces the power of brainwaves at frequencies between 1 and 40 Hz compared to the LA group, but only diazepam (DZP) was able to decrease the intensity of oscillations. The muscle contraction power also indicated a difference in the effectiveness of the three treatments. CONCLUSION: The sum of the evidence indicates that LA causes status epilepticus and that DZP is the most effective treatment for the control of these seizures, by restoring the systemic values to levels close to those recorded in the control group.

Significados atribuídos à sistematização da assistência de Enfermagem: repercussões para o cuidado de Enfermagem e em saúde / Meanings attributed to the systematization of Nursing care: repercussions for Nursing and health care

Teve-se como tese nesta pesquisa que as múltiplas condições hospitalares influenciam a forma como os enfermeiros significam a aplicação da Sistematização da Assistência de Enfermagem (SAE), repercutindo no cuidado de enfermagem e em saúde. Desse modo, objetivou-se conhecer os significados da aplicação da SAE à luz dos enfermeiros; relacionar os achados com o cuidado de enfermagem; e, propor uma teoria que elucide a repercussão da SAE junto ao cuidado em saúde. Pesquisa do tipo explicativa, de abordagem qualitativa, que teve como referenciais teórico e metodológico, respectivamente, o Interacionismo Simbólico e a Teoria Fundamenta nos Dados (TFD). Participaram dezessete enfermeiros assistenciais que atuavam em unidades de internação clínico-cirúrgica adulto em um hospital privado na cidade do Rio de Janeiro/ Brasil, compondo o grupo amostral. A coleta de dados foi realizada por meio de entrevista semiestruturada e observação participante, no período de setembro de 2020 a janeiro de 2021. A seleção dos participantes seguiu os princípios da Teoria Fundamentada nos Dados. A pesquisa atendeu aos aspectos éticos, sendo submetida e aprovada por comitê de ética em pesquisa. A partir da análise dos dados levantados, identificou-se o fenômeno central "Organizando a prática clínico-assistencial: práxis de enfermagem", que representa o cotidiano de trabalho dos enfermeiros ao longo de um plantão hospitalar. Compreendeu-se que múltiplas condições hospitalares repercutem no cuidado de enfermagem e em saúde, e por consequência em como significam a aplicação da Sistematização da Assistência de Enfermagem. São exemplos dessas condições hospitalares: a forma do recebimento e da passagem do plantão hospitalar; as numerosas ferramentas que utilizam para organizar as atividades diretamente ou indiretamente ligadas à sua prática clínico-assistencial; as conferências e registros diários de materiais, equipamentos e insumos; o dimensionamento de pessoal; e a comunicação clínica que estabelecem, dentre outros aspectos. Conclui-se que o papel de gestor de cada unidade de internação em que atuam no cenário pesquisado, uma vez considerando o tempo de demandas imediatas e mediatas, parece se sobrepor ao seu papel de terapeuta à luz dos diagnósticos, dos resultados e das intervenções, impactando em como significam a aplicação da Sistematização da Assistência de Enfermagem a partir dos pilares e dos elementos constituintes destes.

The thesis of this research was that the multiple hospital conditions influence the way nurses signify the application of the Systematization of Nursing Care (SAE), impacting on nursing care and health. Thus, the objective was to know the meanings of the application of SAE in the light of nurses; relate the findings to nursing care; and, to propose a theory that elucidates the repercussion of SAE in health care. Explanatory research, with a qualitative approach, which had as theoretical and methodological references, respectively, the Symbolic Interactionism and the Grounded Theory in Data (GT). Seventeen care nurses who worked in adult clinical-surgical inpatient units in a private hospital in the city of Rio de Janeiro/ Brazil participated, composing the sample group. Data collection was carried out through semi-structured interviews and participant observation, from September 2020 to January 2021. The selection of participants followed the principles of Grounded Theory. The research met the ethical aspects, being submitted and approved by the research ethics committee. From the analysis of the collected data, the central phenomenon was identified "Organizing clinical-care practice: nursing praxis", which represents the daily work of nurses during a hospital shift. It was understood that multiple hospital conditions have an impact on nursing and health care, and consequently on how they mean the application of the Systematization of Nursing Care. Examples of these hospital conditions are: the way in which the hospital shift is received and transferred; the numerous tools they use to organize activities directly or indirectly linked to their clinical-care practice; daily checks and records of materials, equipment and supplies; the dimensioning of personnel; and the clinical communication they establish, among other aspects. It is concluded that the role of manager of each hospitalization unit in which they work in the researched scenario, once considering the time of immediate and mediate demands, seems to overlap with their role as therapist in the light of diagnoses, results and interventions, impacting on how they mean the application of the Systematization of Nursing Care from the pillars and their constituent elements.

La tesis de esta investigación fue que las múltiples condiciones hospitalarias influyen en la forma en que los enfermeros significan la aplicación de la Sistematización de la Atención de Enfermería (SAE), impactando en el cuidado de enfermería y en la salud. Así, el objetivo fue conocer los significados de la aplicación del SAE a la luz de los enfermeros; relacionar los hallazgos con el cuidado de enfermería; y, proponer una teoría que esclarezca la repercusión del SAE en el cuidado de la salud. Investigación explicativa, con abordaje cualitativo, que tuvo como referentes teóricos y metodológicos, respectivamente, el Interaccionismo Simbólico y la Grounded Theory in Data (GT). Participaron diecisiete enfermeros asistenciales que actuaban en unidades de hospitalización clínico-quirúrgicas de adultos en un hospital privado de la ciudad de Rio de Janeiro/Brasil, componiendo el grupo de la muestra. La recolección de datos se realizó a través de entrevistas semiestructuradas y observación participante, desde septiembre de 2020 hasta enero de 2021. La selección de los participantes siguió los principios de la Grounded Theory. La investigación cumplió con los aspectos éticos, siendo sometida y aprobada por el comité de ética en investigación. A partir del análisis de los datos recolectados, se identificó el fenómeno central "Organizando la práctica clínico-asistencial: la praxis de enfermería", que representa el trabajo cotidiano de los enfermeros durante un turno hospitalario. Se entendió que múltiples condiciones hospitalarias tienen impacto en la enfermería y en la atención a la salud, y consecuentemente en cómo significan la aplicación de la Sistematización de la Atención de Enfermería. Ejemplos de estas condiciones hospitalarias son: la forma de recibir y pasar el turno hospitalario; las numerosas herramientas que utilizan para organizar actividades directa o indirectamente vinculadas a su práctica clínico-asistencial; controles y registros diarios de materiales, equipos y suministros; el dimensionamiento del personal; y la comunicación clínica que establecen, entre otros aspectos. Se concluye que el rol de gestor de cada unidad de hospitalización en la que actúan en el escenario investigado, una vez considerado el tiempo de demandas inmediatas y mediatas, parece superponerse con su rol de terapeuta a la luz de los diagnósticos, resultados e intervenciones, impactando sobre cómo significan la aplicación de la Sistematización de la Atención de Enfermería a partir de los pilares y sus elementos constitutivos.

Implicações da Sistematização da Assistência de Enfermagem na prática profissional brasileira / Implicaciones de la Sistematización de la Atención de la Enfermería en la práctica profesional brasileña / Implications of Nursing Care Systematization in Brazilian professional practice

RESUMO Analisar as implicações da Sistematização da Assistência de Enfermagem na prática profissional brasileira. Estudo teórico e de caráter reflexivo, que elencou como categorias analíticas o marco regulador da sistematização, as implicações na prática profissional, os limites e as possibilidades. Identificou-se nos marcos legais uma evolução da compreensão sobre a Sistematização, o que resultou em atualização na resolução que trata da temática. Ressalta-se que as implicações na prática profissional podem ser elucidadas quando forem melhor compreendidos os conceitos de método, pessoal e instrumentos, os três pilares da Sistematização, uma vez que eles não possuem definições conceituais e operacionais na atual resolução. A falta de clareza conceitual acerca do termo, bem como a escassez de definições conceituais e operacionais de seus pilares e de elementos constituintes, têm dificultado a compreensão da Sistematização da Assistência de Enfermagem, impactando na percepção de sua contribuição à prática profissional, visto que não raramente é utilizada como sinônimo de Processo de Enfermagem. A produção de conhecimento acerca da Sistematização deve considerar seus limites e possibilidades. Dessa forma, contribuindo para a demarcação da produção de conhecimento que seja representativa desse objeto epistemológico.

RESUMEN El objetivo es analizar las implicaciones de la Sistematización de la Atención de Enfermería en la práctica profesional brasileña. Se trata de un estudio teórico y reflexivo que enumeró como categorías analíticas, el marco normativo de la sistematización, las implicaciones en la práctica profesional, sus límites y posibilidades. Se identificó una evolución de la comprensión sobre la Sistematización en los marcos legales, trayendo consigo una actualización de la resolución que trata sobre el tema. Cabe destacar que las implicaciones en la práctica profesional pueden dilucidarse cuando se entienden mejor los conceptos del método, del personal y de los instrumentos, los tres pilares de la Sistematización, ya que no existen definiciones conceptuales y operativas en la resolución actual. La falta de claridad conceptual sobre el término, así como la escasez de definiciones conceptuales y operativas de sus pilares y elementos constituyentes, han dificultado la comprensión de la Sistematización de la Atención de Enfermería, impactando en la percepción de su aporte a la práctica profesional, ya que muchas veces se utiliza como sinónimo de Proceso de Enfermería. La producción de conocimiento sobre la Sistematización debe considerar sus límites y posibilidades, contribuyendo, así, a la demarcación de la producción de conocimiento que represente a este objeto epistemológico.

ABSTRACT To analyze the implications of the Nursing Care Systematization in Brazilian professional practice. Theoretical and reflective study, which listed as analytical categories the regulatory framework of systematization, the implications for professional practice, the limits and possibilities. In the legal frameworks, an evolution of understanding about Systematization was identified, which resulted in an update in the resolution that deals with the theme. It is emphasized that the implications for professional practice can be elucidated when the concepts, method, personnel and instruments are better understood, the three pillars of systematization, since they do not have conceptual and operational definitions in the current resolution. The lack of conceptual clarity about the term, as well as the lack of conceptual and operational definitions of its pillars and constituent elements, have made the understanding of the Nursing Care Systematization difficult, impacting the perception of its contribution to professional practice, since it is not rarely used as a synonym for the Nursing Process. The production of knowledge about Systematization must consider its limits and possibilities. Thus, contributing to the demarcation of the production of knowledge that is representative of this epistemological object.

Reflexões sobre o Processo de Enfermagem no trabalho de enfermeiras frente à pandemia da Covid-19 / Reflections on the nursing process in the work of nurses in front of the COVID-19 pandemic

Objetivo: refletir sobre a aplicação do Processo de Enfermagem na organização do cuidado profissional no enfrentamento da COVID-19 no Brasil. Método: estudo de reflexão teórica apoiado na Resolução Cofen nº 358/2009 que dispõe sobre o Processo de Enfermagem. Resultados: são apresentadas considerações sobre a aplicabilidade do Processo de Enfermagem direcionado pelo saber/fazer profissional da equipe de enfermagem sob a liderança da enfermeira. Conclusão: o Processo de Enfermagem se mostra essencial na organização da prática profissional da enfermeira possibilitando sistematizar as ações no enfrentamento da COVID-19 no cenário nacional, subsidiar o registro dos achados clínicos de enfermagem, que possibilitarão gerar dados epidemiológicos, além de refletir o pensamento crítico-reflexivo da enfermeira. (AU)

Objective: To reflect on the application of the Nursing Process in the organization of professional care in coping with Covid-19 in Brazil. Method: Theoretical reflection study supported by Resolution COFEN nº 358/2009, which provides for the Nursing Process. Results: Considerations arepresented about the applicability of the Nursing Process guided by the professional knowledge/practice of the nursing team under the leadership of the nurse. Conclusion: The Nursing Process proves to be essential in the organization of professional nursing practice, making it possible to systematize actions in the fight against Covid-19in the national scenario, to subsidize the registration of clinical nursing findings, which will make it possible to generate epidemiological data, in addition to reflecting the thinking critical-reflective of the nurse. (AU)

Objetivo: Reflexionar sobre la aplicación del Proceso de Enfermería en la organización de la atención profesional para hacer frente a Covid-19en Brasil. Método: Estudio de reflexión teórica respaldado por la Resolución Cofen nº 358/2009, que prevé el Proceso de Enfermería. Resultados: Se presentan consideraciones sobre la aplicabilidad del Proceso de Enfermería guiado por el conocimiento/práctica profesional del equipo de enfermería bajo el liderazgo de la enfermera. Conclusión: El Proceso de Enfermería demuestra ser esencial en la organización de la práctica profesional de enfermería, permitiendo sistematizar acciones en la lucha contra Covid-19 en el escenario nacional, para subsidiar el registro de hallazgos clínicos de enfermería, lo que permitirá generar datos epidemiológicos, además de reflejar el pensamiento. crítico-reflexivo de la enfermera. (AU)

Qualification of palliative nursing assistance in the use of the subcutaneous route.

OBJECTIVES: to describe the experience of conducting workshops for teaching the subcutaneous fluid infusion therapy in palliative care patients. METHODS: experience report based on four workshops with a workload of nine hours each, addressing the teaching, implementation of the technique, and management in the use of subcutaneous fluid infusion therapy in patients in palliative care. The host institution was a private hospital, which had two care units in the state of Rio de Janeiro. RESULTS: we identified little knowledge about the theme. Due to the dynamics used, the workshops made it possible to qualify the participants to perform and manage the subcutaneous route in palliative care environments. CONCLUSIONS: the workshops were an important means of training, qualification, and dissemination of nursing care in a palliative care environment. The resources used to enable the qualification in the execution and management of the presented technique.