RESUMO
Detection of hepatitis B virus (HBV) DNA is particularly important for detection of early acute and of occult HBV infection. On the other hand, HBV DNA detection and quantification are essential to diagnose and treat chronic HBV infection. In this study, we evaluated the performance of the real-time PCR SUMASIGNAL VHB (un paso) (Immunoassay Center, Cuba). The clinical and analytical specificity of the assay was 100%. Intra-assay and inter-assay coefficients of variation ranged from 0.50 to 2.53% and from 1.23 to 3.03%, respectively. A strong correlation (r=0.926; P<0.001) with the COBAS® AmpliPrep/COBAS® TaqMan® HBV Test, v2.0 (Roche Molecular Systems, Inc.) was obtained. The limit of detection using the third WHO international standard for HBV DNA was 377.47 IU/mL. The test was able to detect the most prevalent HBV genotypes (A-G) equally well. In conclusion, the SUMASIGNAL VHB (un paso) is a sensitive, specific, precise and accurate assay for the quantification of serum and plasma HBV DNA. Thus, this simple and fast real-time PCR test can be used as an aid in diagnosing an HBV infection and monitoring drug efficacy.
Assuntos
DNA Viral/sangue , Vírus da Hepatite B/genética , Hepatite B/sangue , Hepatite B/diagnóstico , Hepatite B/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , DNA Viral/genética , Feminino , Humanos , MasculinoRESUMO
Introducción: la infección por el virus de la hepatitis B constituye uno de los principales problemas de salud a nivel mundial, su espectro clínico es variable.Objetivo: Identificar infección oculta y coinfección viral en pacientes con hepatopatía crónica por virus B. Material y Métodos: se realizó un estudio observacional descriptivo transversal en pacientes con hepatitis B crónica con carga viral detectable, atendidos durante el último trimestre de 2014 en el Instituto de Gastroenterología. Se estimó la seroprevalencia de anti HVC y anti HIV así como la frecuencia de AgHBs, y Anti S, las asociaciones fueron evaluadas mediante el estadígrafo x2. Resultados: existió un predominio de las cargas virales bajas para 81.3 por ciento. El 28.7 por ciento de las muestras fueron AgHBs negativas, sugestivas de infección por virus oculto. Se detectaron niveles de protección frente al antígeno S en 20 % de los pacientes con carga viral baja. la coinfección a hepatitis C fue frecuente para 8.4 por ciento. Conclusiones: la infección oculta al virus de hepatitis B fue frecuente en las muestras procesadas en el Instituto de Gastroenterología, con asociación a coinfección al virus de hepatitis C(AU)
Introduction: infection with the hepatitis B virus is one of the major health problems worldwide, clinical spectrum is variable. Objective: to identify occult viral infection and co-infection in patients with chronic liver disease for virus B. Material and Methods: a cross-sectional observational study was conducted in patients with chronic hepatitis B with detectable viral load seen during the last quarter of 2014 in the Institute of Gastroenterology. The seroprevalence of anti HCV and anti HIV and the frequency of HBsAg and Anti S was estimated associations were evaluated using the x2 statistic. Results: there is a predominance of low viral loadsto81.3 percent. 28.7 percent of samples were HBsAg negative, suggestive of occult virus infection. Protective levels against the antigen S were detected in 20 percent of patients with low viral load.Hepatitis C coinfection was frequent for 8.4 percent. Conclusions: the occult infection hepatitis B virus is common in samples processed at the Institute of Gastroenterology with associated coinfection virus hepatitis C(AU)
