Interplay of immune modulation, adaptive response and hormesis: Suggestive of threshold for clinical manifestation of effects of ionizing radiation at low doses?

The health impacts of low-dose ionizing radiation exposures have been a subject of debate over the last three to four decades. While there has been enough evidence of "no adverse observable" health effects at low doses and low dose rates, the hypothesis of "Linear No Threshold" continues to rule and govern the principles of radiation protection and the formulation of regulations and public policies. In adopting this conservative approach, the role of the biological processes underway in the human body is kept at abeyance. This review consolidates the available studies that discuss all related biological pathways and repair mechanisms that inhibit the progression of deleterious effects at low doses and low dose rates of ionizing radiation. It is pertinent that, taking cognizance of these processes, there is a need to have a relook at policies of radiation protection, which as of now are too stringent, leading to undue economic losses and negative public perception about radiation.

Dosimetry audit in advanced radiotherapy using in-house developed anthropomorphic head & neck phantom.

The treatment of head and neck (H&N) cancer presents formidable challenges due to the involvement of normal tissue and organs at risk (OARs) in the close vicinity. Ensuring the precise administration of the prescribed dose demands prior dose verification. Considering contour irregularity and heterogeneity in the H&N region, an anthropomorphic and heterogeneous H&N phantom was developed and fabricated locally for conducting the dosimetry audit in advanced radiotherapy treatments. This specialized phantom emulates human anatomy and incorporates a removable cylindrical insert housing a C-shaped planning target volume (PTV) alongside key OARs including the spinal cord, oral cavity, and bilateral parotid glands. Acrylonitrile Butadiene Styrene (ABS) was chosen for PTV and parotid fabrication, while Delrin was adopted for spinal cord fabrication. A pivotal feature of this phantom is the incorporation of thermoluminescent dosimeters (TLDs) within the PTV and OARs, enabling the measurement of delivered dose. To execute the dosimetry audit, the phantom, accompanied by dosimeters and comprehensive guidelines, was disseminated to multiple radiotherapy centers. Subsequently, hospital physicists acquired computed tomography (CT) scans to generate treatment plans for phantom irradiation. The treatment planning system (TPS) computed the anticipated dose distribution within the phantom, and post-irradiation TLD readings yielded actual dose measurements. The TPS calculated and TLD measured dose values at most of the locations inside the PTV were found comparable within ± 4%. The outcomes affirm the suitability of the developed anthropomorphic H&N phantom for precise dosimetry audits of advanced radiotherapy treatments.

A Clinical Conundrum with Diagnostic and Therapeutic Challenge: a Tale of Two Disorders in One Case.

Living organisms are exposed to exogenous and endogenous agents that affect genomic integrity by creating DNA double strand breaks (DSBs). These breaks are repaired by DNA repair proteins to maintain homeostasis. Defects in DNA repair pathways also affect lymphocyte development and maturation, as DSB sites are critical intermediates for rearrangements required for V(D)J recombination. Recent classifications for inborn errors of immunity (IEIs) have listed DNA repair defect genes in a separate group, which suggests the importance of these genes for adaptive and innate immunity. We report an interesting case of a young female (index P1) with mutations in two different genes, DCLRE1C and FANCA, involved in DNA repair pathways. She presented with clinical manifestations attributed to both defects. With the advent of NGS, more than one defect is increasingly identified in patients with IEIs. Familial segregation studies and appropriate functional assays help ascertain the pathogenicity of these mutations and provide appropriate management and genetic counseling.

A machine learning approach for correcting glow curve anomalies in CaSO4:Dy-based TLD dosimeters used in personnel monitoring.

The study presents a novel approach to analysing the thermoluminescence (TL) glow curves (GCs) of CaSO4:Dy-based personnel monitoring dosimeters using machine learning (ML). This study demonstrates the qualitative and quantitative impact of different types of anomalies on the TL signal and trains ML algorithms to estimate correction factors (CFs) to account for these anomalies. The results show a good degree of agreement between the predicted and actual CFs, with a coefficient of determination greater than 0.95, a root mean square error less than 0.025, and a mean absolute error less than 0.015. The use of ML algorithms leads to a significant two-fold reduction in the coefficient of variation of TL counts from anomalous GCs. This study proposes a promising approach to address anomalies caused by dosimeter, reader, and handling-related factors. Furthermore, it accounts for non-radiation-induced TL at low dose levels towards improving the dosimetric accuracy in personnel monitoring.

Establishment of ex vivo calibration curve for X-ray induced "dicentric + ring" and micronuclei in human peripheral lymphocytes for biodosimetry during radiological emergencies, and validation with dose blinded samples.

In the modern developing society, application of radiation has increased extensively. With significant improvement in the radiation protection practices, exposure to human could be minimized substantially, but cannot be avoided completely. Assessment of exposure is essential for regulatory decision and medical management as applicable. Until now, cytogenetic changes have served as surrogate marker of radiation exposure and have been extensively employed for biological dose estimation of various planned and unplanned exposures. Dicentric Chromosomal Aberration (DCA) is radiation specific and is considered as gold standard, micronucleus is not very specific to radiation and is considered as an alternative method for biodosimetry. In this study dose response curves were generated for X-ray induced "dicentric + ring" and micronuclei, in lymphocytes of three healthy volunteers [2 females (age 22, 23 years) and 1 male (24 year)]. The blood samples were irradiated with X-ray using LINAC (energy 6 MV, dose rate 6 Gy/min), in the dose range of 0-5Gy. Irradiated blood samples were cultured and processed to harvest metaphases, as per standard procedures recommended by International Atomic Energy Agency. Pooled data obtained from all the three volunteers, were in agreement with Poisson distribution for "dicentric + ring", however over dispersion was observed for micronuclei. Data ("dicentric + ring" and micronuclei) were fitted by linear quadratic model of the expression Y[bond, double bond]C + αD + ßD2 using Dose Estimate software, version 5.2. The data fit has resulted in linear coefficient α = 0.0006 (±0.0068) "dicentric + ring" cell-1 Gy-1 and quadratic coefficient ß = 0.0619 (±0.0043) "dicentric + ring" cell-1 Gy-2 for "dicentric + ring" and linear coefficient α = 0.0459 ± (0.0038) micronuclei cell-1 Gy-1 and quadratic coefficient ß = 0.0185 ± (0.0010) micronuclei cell-1 Gy-2 for micronuclei, respectively. Background frequencies for "dicentric + ring" and micronuclei were 0.0006 ± 0.0004 and 0.0077 ± 0.0012 cell-1, respectively. Established curves were validated, by reconstructing the doses of 8 dose blinded samples (4 by DCA and 4 by CBMN) using coefficients generated here. Estimated doses were within the variation of 0.9-16% for "dicentric + ring" and 21.7-31.2% for micronuclei respectively. These established curves have potential to be employed for biodosimetry of occupational, clinical and accidental exposures, for initial triage and medical management.

Measurement of dose enhancement factor for Xoft Axxent electronic brachytherapy device using nanoparticle-embedded alginate film and radiochromic film.

Aim: Inw nanoparticles-aided radiotherapy, the radiation sensitivity of tumor is increased with the infusion of nanoparticles in tumor. This therapeutic modality is capable of delivering enhanced dose to tumor, without exceeding the normal tissue tolerance dose. Further, the quantification of the enhanced dose using suitable dosimeter is important. The present study is aimed at measuring the dose enhancement factors (DEFs) using the combination of nanoparticles-embedded alginate (Alg) film and unlaminated Gafchromic EBT3 film. Materials and Methods: Gold nanoparticles (AuNPs)- and silver nanoparticles (AgNPs)-embedded Alg polymer films were synthesized and characterized using standard techniques. Further, a customized version of the Gafchromic EBT3 film, i.e., unlaminated EBT3 film, was specially fabricated. The DEFs were measured using Xoft Axxent electronic brachytherapy device. Results: The surface plasmon resonance (SPR) and particle size of AuNPs were found to be 550 and 15 ± 2 nm, respectively. In the case of AgNPs, the SPR and particle size were recorded as 400 and 13 ± 2 nm, respectively. The DEFs measured, using unlaminated EBT3 film, for Xoft Axxent electronic brachytherapy using AuNPs and AgNPs were 1.35 ± 0.02 and 1.20 ± 0.01, respectively. Conclusion: The increase in dose enhancement during nanoparticles-aided electronic brachytherapy can be attributed to dominance of photoelectric effect, due to the presence of low-energy X-rays. The investigation indicates that the Xoft Axxent electronic brachytherapy device is suitable for nanoparticles-aided brachytherapy.

Generation of map on natural environmental background absorbed dose rate in India.

A systematic mapping of natural absorbed dose rate was carried out to assess the existing exposure situation in India. The mammoth nationwide survey covered the entire terrestrial region of the country comprising of 45127 sampling grids (grid size 36 km2) with more than 100,000 data points. The data was processed using Geographic Information System. This study is based on established national and international approaches to provide linkage with conventional geochemical mapping of soil. Majority (93%) of the absorbed dose rate data was collected using handheld radiation survey meters and remaining were measured using environmental Thermo Luminescent Dosimeters. The mean absorbed dose rate of the entire country including several mineralized regions, was found to be 96 ± 21 nGy/h. The median, Geometric Mean and Geometric Standard Deviation values of absorbed dose rate were 94, 94 and 1.2 nGy/h, respectively. Among the High Background Radiation Areas of the country, absorbed dose rate varied from 700 to 9562 nGy/h in Karunagappally area of Kollam district, Kerala. The absorbed dose rate in the present nationwide study is comparable with the global database.

ESTIMATION OF PERSONAL DOSE EQUIVALENT HP(0.07) USING CASO4:DY TEFLON DISC-BASED EXTREMITY DOSEMETER.

The radiation workers in India use extremity dosemeter in occupational areas where there is likelihood of receiving significant dose to extremities of the body. Algorithm used for the estimation of whole body dose and extremity dose of wrist are same and is based on slab phantom calibration. However, internationally, different phantoms are recommended for calibration of dosemeter used for extremity and whole body. The recommended quantity for estimation of dose to extremity is the personal dose equivalent Hp(0.07) at 0.07 mm depth. In light of this, an algorithm was developed for estimation of wrist dose in terms of Hp(0.07) based on calibration performed on ISO recommended pillar phantom. Performance of the dosemeter for the estimation of Hp(0.07) with new algorithm was carried out at different angular exposures of photon beams and mixed field of photon and beta. Comparison of results obtained for two types of ISO phantoms (slab and pillar) is also performed for photon beams to highlight the uncertainty caused due to the use of the slab phantom. In case of beta radiation, it was found that the size and shape of the phantom has very little influence in the response of the dosemeter. Performance of the dosemeter using ISO slab and ISO pillar phantoms for beta radiation using the prevalent algorithm was carried out and found to be within the uncertainty limits laid down by ISO except at angle 60°.

Establishment of in vitro Calibration Curve for 60Co-γ-rays Induced Phospho-53BP1 Foci, Rapid Biodosimetry and Initial Triage, and Comparative Evaluations With γH2AX and Cytogenetic Assays.

A rapid and reliable method for biodosimetry of populations exposed to ionizing radiation in the event of an incident or accident is crucial for initial triage and medical attention. DNA-double strand breaks (DSBs) are indicative of radiation exposure, and DSB-repair proteins (53BP1, γH2AX, ATM, etc.) are considered sensitive markers of DSB quantification. Phospho-53BP1 and γH2AX immunofluorescence technique serves as a sensitive, reliable, and reproducible tool for the detection and quantification of DSB-repair proteins, which can be used for biological dose estimations. In this study, dose-response curves were generated for 60Co-γ-rays induced phospho-53 Binding Protein 1 (phospho-53BP1) foci at 1, 2, 4, 8, 16, and 24 h, post-irradiation for a dose range of 0.05-4 Gy using fluorescence microscopy. Following ISO recommendations, minimum detection limits (MDLs) were estimated to be 16, 18, 25, 40, 50, and 75 mGy for dose-response curves generated at 1, 2, 4, 8, 16, and 24 h post-irradiation. Colocalization and correlation of phospho-53BP1 and γH2AX were also measured in irradiated peripheral blood lymphocytes (PBLs) to gain dual confirmation. Comparative evaluation of the established curve was made by γH2AX-immunofluorescence, dicentric chromosome assay (DCA), and reciprocal translocation (RT) assays by reconstructing the dose of 6 dose-blinded samples. Coefficients of respective in-house established dose-response curves were employed to reconstruct the blind doses. Estimated doses were within the variation of 4.124%. For lower doses (0.052 Gy), phospho-53BP1 and γH2AX assays gave closer estimates with the variation of -4.1 to + 9% in comparison to cytogenetic assays, where variations were -8.5 to 24%. For higher doses (3 and 4 Gy), both the cytogenetic and immunofluorescence (phospho-53BP1 and γH2AX), assays gave comparable close estimates, with -11.3 to + 14.3% and -10.3 to -13.7%, variations, respectively.

A Quick Laboratory Method for Assessment of Blood Penetration and Splash Resistance of PPE Fabrics During the COVID-19 Pandemic Situation.

In the current outbreak of COVID-19, healthcare facilities are hit by a shortage of supply of Personal Protective Equipments (PPE) owing to extensive local and global demands and restrictions on their import or export. To circumvent this, trials with several indigenous materials suitable to qualify for PPEs and sterilization techniques for their reuse are being carried out. Prior to their commercialisation, it is imperative to evaluate the resistance of the PPE fabrics against penetration of synthetic blood under applied pressure, 40-300 mmHg as per test standards. Generally, two types of tests are recommended, Penetration Test and Splash Resistance Test, the former being more stringent. While the final certification of PPEs is carried out by authorised agencies, a first impression quick estimate of the choice of fabric can be made using a simple laboratory set-up. This study describes setups developed in the laboratory to carry out these tests. Evaluation of the fabrics, post-gamma irradiation, was also carried out. Microscopic examinations were performed to investigate radiation-induced structural changes in fabrics showing degraded performance. This set-up is useful for selection of fabrics and to assess the feasibility of reuse of PPEs, which is the need of the hour in this pandemic situation.

Investigations on baseline levels for natural radioactivity in soils, rocks, and lakes of Larsemann Hills in East Antarctica.

A comprehensive measurement of concentrations of the natural radionuclides 238U, 232Th and 40K, and 226Ra in the soil and rocks along with natural uranium and tritium activity levels in lake water were carried out during the Indian expedition to Antarctica. The samples were collected from the Larsemann Hills region in Antarctica (latitude 69°20' S to 69°25'S, longitude 76°6' E to 76°23'E). The data on the natural radioactivity for this region is limited. The study was carried out to establish baseline levels of radioactivity in different terrestrial matrices of this region such as soil, rocks, and lake water. A radiation survey mapping for terrestrial radioactivity was conducted in the region before collection of soil and rock samples. The soil and rock samples were analyzed for natural radioactivity concentrations using high-resolution gamma spectroscopy system. The major contributor to elevated gamma radiation background is attributed to the higher concentration of 232Th and 40K radionuclides in both soil and rocks. Terrestrial components of gamma dose rate due to natural radioactivity have been estimated from the measured radioactivity concentrations and dose conversion coefficients. Several "hotspots" and high background areas in the region have been identified having significantly higher concentration of 232Th and 40K. Rocks in Larsemann Hills region showed high reserve of thorium mineralization in monazites and 40K in K-feldspar. The concentrations of 232Th in soil are found to be in the range of 106-603 Bq/kg, whereas in rock it is in the range of 8-4514 Bq/kg. Natural radioactivity U (nat) and 3H contents in the lake water samples in Larsemann Hills region were estimated as 0.4 and 1.3 Bq/L and are well within the prescribed limit of radioactivity in drinking water as recommended by World Health Organization.

Surface dose rate variations in planar and curved geometries of 106Ru/106Rh plaque sources for ocular tumors.

PURPOSE: Investigation of surface dose rate variation with respect to the source configuration of 106Ru/106Rh eye plaque. To explore an alternate way to determine activity of brachytherapy plaques. METHODS: The surface dose rates of 106Ru/106Rh plaque developed indigenously were measured by extrapolation chamber. To rule out possibility of any error in the activity distribution and quantity, same source was used in two different configurations namely planar and curved. EBT3 Gafchromic film was used for determination of uniformity in activity. Monte Carlo-based Codes EGSnrc and FLUKA were used to calculate dose rate in tissue, percentage depth dose and for determination of activity. Parameters and correction factors were estimated using simulations. RESULTS: The measured reference absorbed dose rates for planar and curved 106Ru/106Rh eye plaques are found to be 589 ± 29 mGy/h and 560 ± 28 mGy/h, respectively. The difference in the reference absorbed dose rate of curved eye plaque is about ~5% as compared to planar configuration. The FLUKA-calculated dose values are almost independent of cavity length of the extrapolation chamber for both eye plaques. The FLUKA-based dose rates per µCi 106Ru/106Rh are about 17.28 ± 0.08 mGy/h and 16.48 ± 0.06 mGy/h, respectively for planar and curved eye plaques which match well with the measurements. The calculated activities for planar and curved eye plaques are 34.08 µCi and 33.98 µCi, respectively. CONCLUSIONS: Surface dose rates for a prototype 106Ru/106Rh eye plaque with different configurations were estimated using simulations and measured experimentally. An alternate way to determine activity of beta-gamma brachytherapy plaque has been proposed.

Probability Distribution of Pixel Intensities of EBT3 Films and its Application in the Correction of Uncertainty Budget.

BACKGROUND AND AIM: Modern radiotherapy modalities, such as Intensity-Modulated Radiotherapy and Volumetric Modulated Arc Therapy involve complex dose delivery. The dose delivery is complex as it involves beam modulation, hence, manual dose calculations for these techniques are not possible. Film dosimetry is commonly used method of dose verification for these modalities because of the advantages associated with it. The quantification of uncertainty associated with a film dosimetry system under clinical use becomes important for accurate dosimetry. The spread in the distribution of the pixel values (PV) of the irradiated film contributes to the uncertainty. The probability distribution (PD) of the PV was studied for the clinical photon beam energies of 6, 10, and 15 MV. METHODS AND MATERIALS: Gafchromic EBT3 film and EPSON 10000XL flatbed scanner were used for this purpose and using the resulting PD, the uncertainty budgets for these energies in the red, green and blue color channels were estimated. RESULTS: The PV of exposed films for the energies studied follows t-distribution, the sum of the squares of the deviation of the measured data from the fitted value was of the order of 10-7, this indicates the goodness of fit. The "t" value corrected combined standard uncertainty (CSU) at 1σ confidence level for exposed film and dose measurement at 200 cGy were 1.42%, 1.48%, and 1.63% and 1.99%, 3.23%, and 5.08% for 6, 10, and 15 MV energies, respectively, in the red colour channel. CONCLUSION: In the case of the limited number of measurements of a quantity, the SU values must be corrected using the "t" value to get the correct CSU.

Interaction of cesium bound fission product compounds (CsI and CsOH) with abundant inorganic compounds of atmosphere: Effect on hygroscopic growth properties.

Cesium compounds if present in atmosphere, can affect human health as well as the ecosystem due to their highly hazardous nature. Interaction of cesium compounds with abundantly available atmospheric salts can modify the hygroscopic behavior in sub-saturation relative humidity (RH) domain. Any marked modification in growth factor (GF) for the mixed particle state in comparison to the single particles ultimately affects the settling rates and hence the deposition flux. This work studies the hygroscopic behavior of two important cesium bound fission product aerosols (CsI, CsOH) internally mixed with some common atmospheric particles viz. [Formula: see text] and NaNO3 for a fixed dry particle size of 100 nm. Experimental measurements, performed with Hygroscopic tandem differential mobility analyzer in the range of 20-94% RH, have been compared with the predictions made from Zdanovskii-Stokes-Robinson (ZSR) approach. Apart from the single/pure particle state for the constituents (i.e. mixing ratios 1:0 and 0:1), three other mixing ratios 1:4, 1:1 and 4:1 have been considered. The results show that the GF vs RH pattern for mixed particles is different from that for single CsI and CsOH particles. The intrinsic water uptake behavior for these cesium compounds was found to be perturbed for some of the chosen combinations as well. Deliquescent transition for the mixed particles was observed at lower RH compared to the single electrolytes. Relative differences noticeable for the chosen mixing ratios could be related to the available fractions in the mixed state. Overall, ZSR method was found to be capturing the trend of increasing GFs with increasing RH. Terminal gravitational settling velocities calculated from the measured GFs were also found to be different for single and mixed particles. The relative difference was significant for some combinations and test conditions. Any modification in settling velocity ultimately impacts the deposition flux estimations. Hence neglecting the presence of atmospheric salts affects the accuracy of the source term estimates for a postulated nuclear reactor accident scenario.

Establishment and multiparametric-cytogenetic validation of 60Co-gamma-ray induced, phospho-gamma-H2AX calibration curve for rapid biodosimetry and triage management during radiological emergencies.

Exposure to ionizing radiation is unavoidable to our modern developing society as its applications are widespread and increasing with societal development. The exposures may be planned as in medical applications or may be unplanned as in occupational work and radiological emergencies. Dose quantification of planned and unplanned exposures is essential to make crucial decisions for management of such exposures. This study aims to establish ex-vivo dose-response curve for 60Co-gamma-ray induced gamma-H2AX-foci by immunofluorescence using microscopy and flowcytometry with human lymphocytes. This technique has the potential to serve as a rapid tool for dose estimation and triage application during small to large scale radiological emergencies and clinical exposures. Response curves were generated for the dose range 0-4 Gy (at 1, 2, 4, 8, 16, 24, 48, 72 and 96 h of incubation after irradiation) with microscopy and 0-8 Gy (at 2, 4, 8, 16 and 24 h of incubation after irradiation) with flow cytometry. These curves can be applied for dose reconstruction when post exposure sampling is delayed up to 96 h. In order to evaluate Minimum Detection Limit (MDL) of the assay, variation of background frequency of gamma-H2AX-foci was measured in 12 volunteers. To understand the application window of the assay, gamma-H2AX foci decay kinetics has been studied up to 96 h with microscopy and response curves were generated from 1 to 96 hours post exposure. Gamma-H2AX fluorescence intensity decay kinetics was also studied up to 96 h with flow cytometry and response curves were generated from 2 to 24 hours post irradiation. Established curves were validated with dose blinded samples and also compared with standard cytogenetic assays. An inter-comparison of dose estimates was made among gamma-H2AX assay, dicentric aberrations and reciprocal translocations for application window in various dose ranges and time of blood collection after exposures.

Wire mesh capped DRPS based bronchial dosimeter for personal inhalation dosimetry due to radon progeny.

A study has been carried out to experimentally determine the calibration factor (CF) of the passive bronchial dosimeter, which consists of a direct radon progeny sensor capped with a 100-wire mesh. First, the CF was determined in controlled environmental conditions simulated in a calibration chamber. With aerosol concentrations varying from 104p cm-3to 105p cm-3and relative humidity varying from 60% to 80% in the chamber, CF was observed to be nearly constant with an average value of (3.8 ± 0.5) × 10-3mSv tracks-1cm2. Then, the CF was determined in real indoor environments in which it was again observed to be almost constant and the mean value was found to be (5.6 ± 0.1) × 10-3mSv tracks-1cm2. Pooling all the data on CFs obtained under controlled conditions and in real indoor environments, a lognormal distribution of the CF was observed with a geometric mean and geometric standard deviation of 0.0052 mSv tracks-1cm2and 1.28 respectively. The experimentally determined value of CF was found to be in close agreement with the theoretically estimated value, taking into consideration the unattached fraction of radon progeny. This dosimeter is passive, cheap, lightweight and, moreover, the CF being stable against environmental variations, will be useful in monitoring inhalation doses due to radon progeny for occupational workers.

A novel method based on 220Rn (thoron) exhalation rate of indoor surfaces for robust estimates of 220Rn concentration and equilibrium factor to compute inhalation dose.

The research into 220Rn (thoron) has generated an increasing interest in recent times due to the realisation of its radiological importance in many indoor environments. Though it is assumed that the contribution of 220Rn, per se, to the inhalation dose is negligible in comparison with that of its decay products, this may not be always true. Correct estimation of inhalation dose due to thoron requires a reliable method to measure the concentration of both 220Rn and its decay products in indoor air. However, due to its very short half-life (55.6 s) 220Rn shows large variation in its indoor activity concentration. This makes it difficult to have a robust value of 220Rn concentration which can be considered representative of a house, thus making the dose estimation unreliable. This issue has been addressed in the present study by developing a novel method that utilises the 220Rn exhalation rate from indoor surfaces as the basis for estimation of average 220Rn concentration in indoor air. The 220Rn concentration estimated in this manner can be converted to decay products concentration using a suitable equilibrium factor and finally the inhalation dose using appropriate dose conversion factors. A wall mounting accumulator setup has been developed for easy in-situ measurement of 220Rn exhalation from room surfaces. The method has been validated through comprehensive measurements in 25 dwellings in two different regions of India. The developed method is very good for large scale field surveys because of fast and easy applicability.

A periodic pumping technique of soil gas for 222Rn stabilization in large calibration chambers: part 2-theoretical formulation and experimental validation.

In an adjoining publication, we demonstrated the novel technique to harvest soil gas of natural origin as a highly efficient source of 222Rn for calibration applications in a large volume 222Rn calibration chamber. Its advantages over the use of conventional high strength 226Ra sources, such as the capability to serve as a non-depleting reservoir of 222Rn and achieve the desired concentration inside the calibration chamber within a very short time, devoid of radiation safety issues in source handling and licensing requirements from the regulatory authority, were discussed in detail. It was also demonstrated that stability in the 222Rn concentration in large calibration chambers could be achieved within ± 20% deviation from the desired value through a semi-dynamic mode of injection in which 222Rn laden air was periodically pumped to compensate for its loss due to leak and decay. The necessity of developing a theory for determining the appropriate periodicity of pumping was realized to get good temporal stability with a universally acceptable deviation of ≤ ± 10% in the 222Rn concentration. In this paper, we present a mathematical formulation to determine the injection periods (injection pump ON and OFF durations) for the semi-dynamic operation to achieve long term temporal stability in the 222Rn concentration in the chamber. These computed pumping parameters were then used to efficiently direct the injection of soil gas into the chamber. We present the mathematical formulation, and its experimental validations in a large volume calibration chamber (22 m3). With this, the temporal stability of 222Rn concentration in the chamber was achieved with a deviation of ~ 3% from the desired value.

An innovative technique of harvesting soil gas as a highly efficient source of 222Rn for calibration applications in a walk-in type chamber: part-1.

The paper describes a novel technique to harvest 222Rn laden air from soil gas of natural origin as a highly efficient source of 222Rn for calibration applications in a walk-in type 222Rn calibration chamber. The technique makes use of a soil probe of about 1 m to draw soil gas, through a dehumidifier and a delay volume, using an air pump to fill the calibration chamber. 222Rn concentration in the range of a few hundred Bq m-3 to a few tens of kBq m-3 was easily attained in the chamber of volume 22.7 m3 within a short pumping duration of 1 h. A new technique referred to as "semi-dynamic mode of operation" in which soil gas is injected into the calibration chamber at regular intervals to compensate for the loss of 222Rn due to decay and leak is discussed. Harvesting soil gas has many important advantages over the traditional methods of 222Rn generation for calibration experiments using finite sources such as solid flow-through, powdered emanation, and liquid sources. They are: (1) soil gas serves as an instantaneous natural source of 222Rn, very convenient to use unlike the high strength 226Ra sources used in the calibration laboratories, and has no radiation safety issues, (2) does not require licensing from the regulatory authority, and (3) it can be used continuously as a non-depleting reservoir of 222Rn, unlike other finite sources. The newly developed technique would eliminate the need for expensive radioactive sources and thereby offers immense application in a variety of day to day experiments-both in students and research laboratories.