A novel non-enzymatic electrochemical uric acid sensing method based on nanohydroxyapatite from eggshell biowaste immobilized on a zinc oxide nanoparticle modified activated carbon electrode (Hap-Esb/ZnONPs/ACE).

Hydroxyapatite-derived eggshell biowaste (Hap-Esb) has been fabricated and developed for the electrochemical detection of uric acid (UA). The physicochemical characteristics of the Hap-Esb and modified electrodes were evaluated using a scanning electron microscope and X-ray Diffraction analysis. Utilized as UA sensors, the electrochemical behavior of modified electrodes (Hap-Esb/ZnONPs/ACE) was assessed using cyclic voltammetry (CV). The superior peak current response observed for the oxidation of UA at Hap-Esb/ZnONPs/ACE, which was 13 times higher than that of the Hap-Esb/activated carbon electrode (Hap-Esb/ACE) is attributed to the simple immobilization of Hap-Esb on zinc oxide nanoparticle-modified ACE. The UA sensor exhibited a linear range at 0.01 to 1 µM, low detection limit (0.0086 µM), and excellent stability, which surpass the existing Hap-based electrodes reported in the literature. The facile UA sensor subsequently realized is also advantaged by its simplicity, repeatability, reproducibility, and low cost, applicable for real sample analysis (human urine sample).

Highly selective electrochemical sensing based on electropolymerized ion imprinted polyaniline (IIPANI) on a bismuth modified carbon paste electrode (CPE-Bi) for monitoring Nickel(ii) in river water.

Electrochemical sensors based on ion-imprinting polymers have emerged as an effective analytical tool for heavy metal tracking. This study describes a simple and facile technique for manufacturing a highly selective and sensitive electrode using an ion imprinting polymer on a bismuth-modified carbon paste electrode. The developed sensor applied aniline as a functional monomer and was used for tracking Ni(ii) ions. The proposed sensor was thoroughly characterized by scanning electron microscopy, cyclic voltammetry, and differential pulse striping anodic voltammetry. The analytical evaluation showed that the proposed sensor has a linear dynamic range (R 2 = 0.999) for the Ni(ii) concentration range of 0.01 to 1 µM and a limit of detection value of 0.00482 µM. The proposed sensor showed excellent performance when tested for tracking Ni(ii) ions in the presence of interfering ions (Cd(ii), Co(ii), Cu(ii), and Zn(ii) ions) at a 1000-fold higher concentration. When the proposed sensor was tested for tracking Ni(ii) concentration in an actual river sample, our modified sensor showed similar results compared to the atomic absorption spectroscopy evaluation (p > 0.05, n = 3). In summary, our proposed sensor is promising for monitoring Ni(ii) ions in the aquatic environment.

Silver Nanoparticles Coupled with Graphene Nanoplatelets Modified Screen-Printed Carbon Electrodes for Rhodamine B Detection in Food Products.

A rapid, simple, and sensitive voltammetric sensor has been fabricated to determine Rhodamine B (RhB), a textile coloring agent. Silver nanoparticles (AgNPs) were synthesized by the chemical reduction method of silver nitrate and sodium citrate. Graphene nanoplatelets (GPLs) and AgNPs were drop-casted on the surface of a working electrode of a screen-printed carbon electrode (SPCE), forming the SPCE-GPLs/AgNPs samples. Scanning electron microscopy-energy dispersive X-ray and cyclic voltammetry confirmed the altered surface of the SPCE. The square wave voltammetry was used for the electrochemical determination of RhB. The SPCE-GPLs/AgNPs demonstrated electrochemical responses to detect RhB with a linear range of 2-100 µM, and the limit of detection was 1.94 µM. The SPCE-GPLs/AgNPs demonstrated a selective detection of RhB in the presence of common interfering compounds present in the food samples, including sucrose and monosodium glutamate. Furthermore, the sensor presented good reproducibility as well as repeatability in the detection of RhB. When the sensor was used to determine RhB in an actual food sample, similar results were shown as suggested by UV-vis spectroscopy analysis. Hence, the fabricated sensor can be applied for the detection of RhB in food samples.

Electrochemical sensor based on screen printed carbon electrode-zinc oxide nano particles/molecularly imprinted-polymer (SPCE-ZnONPs/MIP) for detection of sodium dodecyl sulfate (SDS).

The foremost objective of this work is to prepare a novel electrochemical sensor-based screen-printed carbon electrode made of zinc oxide nanoparticles/molecularly imprinted polymer (SPCE-ZnONPs/MIP) and investigate its characteristics to detect sodium dodecyl sulfate (SDS). The MIP that is polyglutamic acid (PGA) film was synthesized via in situ electro-polymerization. The SDS's recognition site was left on the surface of the PGA film after extraction using the cyclic voltammetry (CV) technique, facilitating the specific detection of SDS. Moreover, the ZnONPs (∼71 nm, polydispersity index of 0.138) were synthesized and effectively combined with the MIP by a drop-casting method, enhancing the current response. The surface of the prepared SPCE-ZnONPs/MIP was characterized by scanning electron microscopy and energy dispersive X-ray. Besides, the electrochemical performance of the SPCE-ZnONPs/MIP was also studied through CV and differential pulse voltammetry (DPV) techniques. As an outstanding result, it is observed that the current response of SPCE-ZnONPs/MIP for detection of SDS remarkably increased almost four times higher from 0.009 mA to 0.041 mA in comparison with bare SPCE. More importantly, the proposed SPCE-ZnONPs/MIP exhibited an excellent selectivity (in the presence of interfering molecules of Ca2+, Pb2+, as well as sodium dodecylbenzene sulfonate (SDBS)), sensitivity, reproducibility, and repeatability. Since the modified sensor offers portability, it is suitable for in situ environment and cosmetic monitoring.

Fractionation using adsorptive macroporous resin HPD-600 enhances antioxidant activity of Gnetum gnemon L. seed hard shell extract.

In this study, antioxidant activities and identification of the bioactive substances in Gnetum gnemon L. (Gg) seed hard shell were evaluated. The seed of Gnetum gnemon L., an Indonesian native plant, is commonly consumed as a vegetable or further processed as cracker. Isolated substances from Gnetum gnemon seed are mainly stilbenoid derivatives which show potent antioxidant, tyrosinase inhibitor, and antimicrobial activities. Nevertheless, the antioxidant activity of its crude extract is still considered weak. In this study, an effort was made to improve antioxidant potency by fractionation using macroporous adsorptive resin (MAR). This fractionation successfully enhanced antioxidant activity of red Gg seed hard shell extract with efficient adsorption contact time within 30 min. Antioxidant activity of fractions 25-75% v/v ethanol increased three- to sevenfold as compared to crude extract and more importantly resulted in dry product which was easier for further processes. Identification of bioactive compounds in Gg seed hard shell extract with different degrees of ripeness was also performed by HPLC and confirmed the presence of Gnetin C, resveratrol, and other stilbenoid derivatives. These other stilbenoid derivatives could be the main substances contributing in antioxidant action with lower IC50 as compared to both Gnetin C and resveratrol. In summary, fractionation process using MAR HPD-600 reduced unnecessary sugar molecules from red Gg seed hard shell extract hence resulting to fraction with strong antioxidant activity.

Antioxidant activity and total phenolic content of endophytic fungus Fennellia nivea NRRL 5504.

Antioxidant is an interesting bioactivity since it has several correlations with disease such as Alzheimer, cancer, ageing and many others in which were promoted by free radicals. Investigation on our endophytic fungus collection namely Fennelia nivea NRRL 5504 revealed that the fermented mediumhas antioxidant property against DPPH through free radical scavenging effect. Fermentation in liquid medium i.e. Potatoes Dextrose Broth, resulted that the fermented medium reached about 86.51% (p < 0.05) of free radical inhibition on the eighth day. Results ofphytochemical screening from extracted fermentation medium showed the presence of terpenes, triterpenes, phenolic compound, tannin, flavonoid and also saponin. The highest antioxidant activity was showed by ethyl acetate extract. Calculation on total phenolic content of ethyl acetate crude extract was 0.544 mg g(-1) equivalent to pyrogallol. By exposure above, we have alternative source of antioxidant that came from our endophytic fungus collection which we isolated previously from Typhonium divaricatum Lodd.