Estudos hematológicos em Oreochromis niloticus (Linnaeus, 1758) (Cichlidae, Teleostei) - Parte I / Hematological studies in Oreochromis niloticus (Linnaeus, 1758) (Cichlidae, Teleostei) - Part I

Avaliou-se o hemograma e a morfologia celular em amostras de sangue periférico obtidas de 10 peixes da espécie Oreochromis niloticus. Parte das mesmas foi utilizada para o estudo da hematimetria, isto é, contagem de eritrócitos (x = 2,35 x 10 elevado à sexta potência/mm ao cubo mais ou menos 0,12), taxa de hemoglobina (x = 7,04g/dL mais ou menos 0,38), hematócrito (x = 27,85 por cento mais ou menos 1,62), HCM (x = 30,31pg mais ou menos 1,64), VCM (x = 118,6u ao cubo mais ou menos 3,53), CHCM (x = 25,78 por cento mais ou menos 1,72), contagem global de leucócitos (x = 16,08 x 10 ao cubo/mm ao cubo mais ou menos 1,28), contagem de trombócitos (x = 61,69 x 10 ao cubo/mm ao cubo mais ou menos 5,96), sendo a avaliaçäo da morfologia das células do sangue realizada através do estudo de extensöes sanguíneas submetidas à coloraçäo de Wright. Os eritrócitos e trombócitos nucleados apresentaram-se elípticos; neutrófilos e eosinófilos esféricos de tamanho variável, com o núcleo também esférico, apresentaram citoplasmas abundantes, ligeiramente basófilo e altamente acidófilo, respectivamente. Näo foram vistas as formas polimorfonucleares dessas células. Os basófilos apresentaram núcleo esférico e granulaçöes metacromáticas. Os monócitos e os linfócitos assemelharam-se morfologicamente aos dos mamíferos

Morphology and morphometry of the cervix uteri of female albino rats in the estrus and diestrus phases.

The morphological aspects of the cervix uteri of twelve albino rats, six in estrus and six in diestrus were studied. Micrometry of the epithelium thickness was performed and it was observed that the epithelium is significantly thicker during the estrus phase of the cycle than in diestrus. The major causes for this are discussed and the findings are correlated with the estrogen and progesterone levels present in each of the mentioned phases.

[Ultrastructural and morphometric study of the myeloid parenchyma cells of mice prior and after X-ray exposure]. / Estudo ultra-estrutural e morfométrico de células do parênquima mielóide de camundongos antes e depois da exposição aos raios X.

An electron microscopic study of initial X-ray changes in ultrastructures of bone marrow cells was performed. Such changes reached nucleous and cytoplasm organellas early (1 hour) after radiation, but were more proeminent later on. Subsequent readings (2, 6 hours) showed them more proeminent in cytoplasm structures, and the morphologic appearance was close to normal within 12, 14 days. Quantitative analyses were performed using the marrow recovery coefficient (MRC).

[Evaluation of the effects of ionizing radiation and cyclophosphamide on the bone marrow of mice, by counting non-nucleated and nucleated cells]. / Avaliação dos efeitos da radiação ionizante e da ciclofosfamida na medula óssea de camundongos, pela contagem das células não nucleadas e nucleadas.

Bone marrow injury after exposure to ionizing radiation and cyclophosphamide was evaluated by morphologic analysis and by the ratio erythrocytes/nucleated cells (NN/N). The authors show the efficiency of the method proposed, which can be determined in bone marrow samples of variable and unpredictable size.

[Ultrastructural aspects of the skeletal striated muscle, macrophages and fibroblasts during the regression of tadpole tails]. / Aspectos ultra-estruturais do músculo estriado esquelético, macrófagos e fibroblastos durante a regressão da cauda de girinos.

Striated muscle fibres and fibroblasts observed at electron microscope were entirely developed when the tail of tadpoles reached its maximum size. However, during resorption of the tail, striated muscle fibres showed signs of degeneration: rupture and disorganization of myofibrils, altered mitochondria and lipid droplets in the cytoplasm. A great amount of macrophages phagocyting myofibrils and fibroblasts containing collagen fibrils in several breakdown stages were also observed among degenerated muscle fibres.