Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study.

AIMS: The aim of the current study was to examine the effects of diode laser (970 nm) when used as adjunct to mechanical debridement on reattachment of fibroblast cell cultures to the root surface. MATERIALS AND METHODS: Eighteen extracted single-rooted human teeth were used in the study. Briefly, the anatomical crowns were removed; roots were sectioned and preserved in antiseptic solution. The sections were divided into three groups: (i) scaling and root planing (SRP), (ii) SRP followed by diode laser exposure and (iii) untreated control group. All the sections were inoculated with NIH 3T3 mouse fibroblast cell lines and cell attachment was confirmed by scanning electron microscopy analysis and growth was quantitatively assessed by MTT assay after days 1, 3 and 5. RESULTS: On intragroup analysis, there was significant cell proliferation seen on day 1 and day 5. However, no significant differences were observed between the treatment groups at the various time points. CONCLUSION: 970-nm diode laser exposure had a positive effect in enhancing the fibroblast reattachment to root surfaces. However, to ascertain the role of laser treatment in enhancing the rapid cell growth compared to SRP, further quantitative studies with more sample numbers are required.

Evaluation of glutathione level in gingival crevicular fluid in periodontal health, in chronic periodontitis and after nonsurgical periodontal therapy: A clinicobiochemical study.

CONTEXT: Periodontitis is predominantly due to exaggerated host response to pathogenic microorganisms and their products which causes an imbalance between the reactive oxygen species-antioxidant in gingival crevicular fluid (GCF). Glutathione is an important redox regulator in GCF and maintenance of stable reduced glutathione (GSH):oxidized glutathione (GSSG) ratio is essential for periodontal health. AIMS: The present study was undertaken to evaluate and compare the level of glutathione and redox balance (GSH: GSSG ratio) in GCF of chronic periodontitis patients, periodontally healthy controls and also to evaluate the effect of nonsurgical periodontal therapy on the level of glutathione and redox balance during 3 months postoperative visit. STUDY DESIGN: Baseline GCF samples were collected from 20 chronic periodontitis patients and 20 periodontally healthy subjects for GSH and GSSG levels estimation. Periodontitis patients were recalled 3 months postnonsurgical periodontal therapy to re-sample GCF. MATERIALS AND METHODS: GSH and GSSG levels were measured by high-performance liquid chromatography. The values were statistically analyzed by Paired t-test. RESULTS: The mean GSH and GSSG values in GCF were found to be significantly lower in periodontitis patients pre- and 3 months post-nonsurgical periodontal therapy, compared with those in the control group subjects. In addition, the successful nonsurgical therapy even though leading to a significant improvement in the GSH and GSSG levels, does not restore glutathione concentration to the levels seen in healthy subjects. CONCLUSION: Successful nonsurgical periodontal therapy leads to significant improvement in the redox balance (GSH: GSSG ratio) in chronic periodontitis patients.