Assessment of Growth, Yield, and Nutrient Uptake of Mediterranean Tomato Landraces in Response to Salinity Stress.

Salinity is a major stress factor that compromises vegetable production in semi-arid climates such as the Mediterranean. The accumulation of salts in the soil can be attributed to limited water availability, which can be exacerbated by changes in rainfall patterns and rising temperatures. These factors can alter soil moisture levels and evaporation rates, ultimately leading to an increase in soil salinity, and, concomitantly, the extent to which crop yield is affected by salinity stress is considered cultivar-dependent. In contrast to tomato hybrids, tomato landraces often exhibit greater genetic diversity and resilience to environmental stresses, constituting valuable resources for breeding programs seeking to introduce new tolerance mechanisms. Therefore, in the present study, we investigated the effects of mild salinity stress on the growth, yield, and nutritional status of sixteen Mediterranean tomato landraces of all size types that had been pre-selected as salinity tolerant in previous screening trials. The experiment was carried out in the greenhouse facilities of the Laboratory of Vegetable Production at the Agricultural University of Athens. To induce salinity stress, plants were grown hydroponically and irrigated with a nutrient solution containing NaCl at a concentration that could maintain the NaCl level in the root zone at 30 mM, while the non-salt-treated plants were irrigated with a nutrient solution containing 0.5 mM NaCl. Various plant growth parameters, including dry matter content and fruit yield (measured by the number and weight of fruits per plant), were evaluated to assess the impact of salinity stress. In addition, the nutritional status of the plants was assessed by determining the concentrations of macro- and micronutrients in the leaves, roots, and fruit of the plants. The key results of this study reveal that cherry-type tomato landraces exhibit the highest tolerance to salinity stress, as the landraces 'Cherry-INRAE (1)', 'Cherry-INRAE (3)', and 'Cherry-INRAE (4)' did not experience a decrease in yield when exposed to salinity stress. However, larger landraces such as 'de Ramellet' also exhibit mechanisms conferring tolerance to salinity, as their yield was not compromised by the stress applied. The identified tolerant and resistant varieties could potentially be used in breeding programs to develop new varieties and hybrids that are better adapted to salinity-affected environments. The identification and utilization of tomato varieties that are adapted to salinity stress is an important strategy for promoting agriculture sustainability, particularly in semi-arid regions where salinity stress is a major challenge.

Comparing the Nutritional Needs of Two Solanaceae and One Cucurbitaceae Species Grown Hydroponically under the Same Cropping Conditions.

Switching over to closed-loop soilless culture systems, thus preventing pollution of water resources by nitrates and saving water and fertilizers, requires accurate estimations of the mean nutrient-to-water uptake ratios. To contribute to this objective, three fruit vegetable species (tomato, eggplant, cucumber) were grown hydroponically in a floating system under identical cropping conditions to quantify species differences in nutrient uptake. The composition of the nutrient solution used to feed the crops was identical for all species. The total water consumption and the concentrations of most nutrients (K, Ca, Mg, N, P, Fe, Mn, Zn, Cu, B) in the nutrient solution and the plant tissues were measured at crop establishment and at two different crop developmental stages. The obtained data were used to determine the uptake concentrations (UCs) using two mass balance models, one based on nutrient removal from the nutrient solution and a second based on nutrient recovery in the plant tissues. The experiment was conducted in the spring-summer season. The results revealed that the nutrient uptake concentrations were substantially different between species for all nutrients except for N, while there were also significant interactions between the two methods used for their estimation of some nutrients. Thus, the UCs of N, P, Ca, and some micronutrients were significantly higher when its estimation was based on the removal of nutrients from the nutrient solution compared to recovery from plant tissues, presumably because with the first method, losses due to denitrification or precipitation could not be separated from those of plant uptake. The comparison of the three greenhouse vegetables revealed a similar UC for nitrogen, while cucumber generally showed significantly lower UCs for P and for the micronutrients Fe, Zn, and Cu at both cropping stages compared to the two Solanaceae species. The obtained results can be used to precisely adjust the nutrient supply in closed-loop soilless cultivations to the plant uptake thus avoiding both depletion and accumulation of nutrients in the root environment.

Combined Effect of Salt Stress and Nitrogen Level on the Primary Metabolism of Two Contrasting Hydroponically Grown Cichorium spinosum L. Ecotypes.

Stamnagathi (Cichorium spinosum L.) is an indigenous plant species well-known for its health-promoting properties. Salinity is a long-term issue with devastating consequences on land and farmers. Nitrogen (N) constitutes a crucial element for plant growth and development (chlorophyll, primary metabolites, etc.). Thus, it is of paramount importance to investigate the impact of salinity and N supply on plants' metabolism. Within this context, a study was conducted aiming to assess the impact of salinity and N stress on the primary metabolism of two contrasting ecotypes of stamnagathi (montane and seaside). Both ecotypes were exposed to three different salinity levels (0.3 mM-non-saline treatment, 20 mM-medium, and 40 mM-high salinity level) combined with two different total-N supply levels: a low-N at 4 mM and a high-N at 16 mM, respectively. The differences between the two ecotypes revealed the variable responses of the plant under the applied treatments. Fluctuations were observed at the level of TCA cycle intermediates (fumarate, malate, and succinate) of the montane ecotype, while the seaside ecotype was not affected. In addition, the results showed that proline (Pro) levels increased in both ecotypes grown under a low N-supply and high salt stress, while other osmoprotectant metabolites such as γ-aminobutyric acid (GABA) exhibited variable responses under the different N supply levels. Fatty acids such as α-linolenate and linoleate also displayed variable fluctuations following plant treatments. The carbohydrate content of the plants, as indicated by the levels of glucose, fructose, α,α-trehalose, and myo-inositol, was significantly affected by the applied treatments. These findings suggest that the different adaptation mechanisms among the two contrasting ecotypes could be strongly correlated with the observed changes in their primary metabolism. This study also suggests that the seaside ecotype may have developed unique adaptation mechanisms to cope with high N supply and salinity stress, making it a promising candidate for future breeding programs aimed at developing stress tolerant varieties of C. spinosum L.

Effects of NaCl and CaCl2 as Eustress Factors on Growth, Yield, and Mineral Composition of Hydroponically Grown Valerianella locusta.

Corn salad (Valerianella locusta) is a popular winter salad, cultivated as an ingredient for ready-to-eat salads. The application of mild salinity stress (eustress) can increase the flavor and reduce the nitrate content of certain crops but, at the same time, a wrong choice of the eustress type and dose can negatively affect the overall productivity. In this research, the effects of different isosmotic salt solutions, corresponding to two different electrical conductivity (EC) levels, were investigated on the yield and mineral composition of hydroponically grown Valerianella locusta "Elixir". Five nutrient solutions (NS) were compared, including a basic NS used as the control, and four saline NS were obtained by adding to the basic NS either NaCl or CaCl2 at two rates each, corresponding to two isosmotic salt levels at a low and high EC level. Corn salad proved moderately susceptible to long-term salinity stress, suffering growth losses at both low and high EC levels of saline solution, except from the low NaCl treatment. Hence, it appears that mild salinity stress induced by NaCl could be employed as an eustress solution and corn salad could be cultivated with low-quality irrigation water (20 mM NaCl) in hydroponic systems.

Hydroponic Common-Bean Performance under Reduced N-Supply Level and Rhizobia Application.

This study aims to explore the possibility of a reduced application of inorganic nitrogen (N) fertiliser on the yield, yield qualities, and biological nitrogen fixation (BNF) of the hydroponic common bean (Phaseolus vulgaris L.), without compromising plant performance, by utilizing the inherent ability of this plant to symbiotically fix N2. Until the flowering stage, plants were supplied with a nutrient solution containing N-concentrations of either a, 100%, conventional standard-practice, 13.8 mM; b, 75% of the standard, 10.35 mM; or c, 50% of the standard, 6.9 mM. During the subsequent reproductive stage, inorganic-N treatments b and c were decreased to 25% of the standard, and the standard (100% level) N-application was not altered. The three different inorganic-N supply treatments were combined with two different rhizobia strains, and a control (no-inoculation) treatment, in a two-factorial experiment. The rhizobia strains applied were either the indigenous strain Rhizobium sophoriradicis PVTN21 or the commercially supplied Rhizobium tropici CIAT 899. Results showed that the 50-25% mineral-N application regime led to significant increases in nodulation, BNF, and fresh-pod yield, compared to the other treatment, with a reduced inorganic-N supply. On the other hand, the 75-25% mineral-N regime applied during the vegetative stage restricted nodulation and BNF, thus incurring significant yield losses. Both rhizobia strains stimulated nodulation and BNF. However, the BNF capacity they facilitated was suppressed as the inorganic-N input increased. In addition, strain PVTN21 was superior to CIAT 899-as 50-25% N-treated plants inoculated with the former showed a yield loss of 11%, compared to the 100%-N-treated plants. In conclusion, N-use efficiency optimises BNF, reduces mineral-N-input dependency, and therefore may reduce any consequential negative environmental consequences of mineral-N over-application.

Enhancing bioactive compounds accumulation in red beet (Beta Vulgaris L.) plants by managing N nutrition. The identification of the 'critical' zone as a cultivation technique.

The increasing interest in natural health-promoting compounds, which are mostly plant secondary metabolites, inspired attempts to stimulate mechanisms strengthening their bioaccumulation in crop plants via abiotic stress while maintaining the yield potential. This study investigates the long-term effects of limiting nitrogen (N) supply on the concentration of total phenolics, free radical activity of natural antioxidants, betacyanin content, biomass production, net photosynthetic rate, total chlorophyll content, and plant water relations in red beetroot plants (Beta vulgaris L.) grown hydroponically. Depending on fertilization, the range of N supply for evaporative demand comprises two contrasted nutrient zones, in which N is limiting (zone-1) or non-limiting (zone-2). Based on the carbon-nutrient-balance hypothesis, at the transition from 1st-zone to 2nd-zone, there is a narrow transition zone in which the plant nutrient status is considered 'critical'. Herein, to determine the 'critical' zone, a modified Michaelis-Menten (M-M) model was used using a piecewise linear regression on two indexes: net photosynthetic rates and free radical-scavenging capacity of phenolic antioxidants. The model showed that the 'critical' transition points of net photosynthetic rate and phenolic free radical content are located in a narrow zone ranging between 196.70 ± 8.75 and 271.54 ± 75.50 ppm NO3-, while the cropping season appears to affect slightly the range of 'critical' (transition) zone. Thus, supplying N to red beetroot plants to levels ranging within this 'critical' zone may be an efficient, profitable and sustainable way to increase the accumulation of health-promoting plant bioactive compounds (total phenolic compounds with radical activity and betacyanins) in hydroponically cultivated reed beetroot plants.

Fresh Pod Yield, Physical and Nutritional Quality Attributes of Common Bean as Influenced by Conventional or Organic Farming Practices.

The aim of the current study wat to comparatively assess the impact of different nitrogen (N) fertilization schemes on fresh pod yield and yield quality in either organically or conventionally grown common beans (Phaseolus vulgaris L.). Prior to common bean crop establishment, the experimental field site was cultivated following either organic (a) or conventional (b) farming practices with a winter non-legume crop (Brassica oleracea var. italica) (BR), or (c) with field bean (Vicia faba sp.) destined to serve as a green manure (GM) crop. At the end of the winter cultivation period the broccoli crop residues (BR) and green manure biomass (GM) were incorporated into the soil and the plots that accommodated the treatments (a) and (c) were followed by an organically cultivated common bean crop, while the conventional broccoli crop was followed by a conventionally cropped common bean crop. Additional to the plant residues (BR), sheep manure (SM) at a rate of 40 kg N ha-1 was also applied to the organically treated common beans, while the plots with a conventionally cropped common bean received 75 kg N ha-1. Organic common bean treated with SM + BR produced smaller pods of higher dry matter and bioactive compound content, responses that are correlated with limited soil N availability. No significant variations were observed on yield components and N levels of pods cultivated under organic (SM + GM) and conventional cropping systems. Pod sugar and starch content was not influenced by the different fertilization practices. In conclusion, we have demonstrated that the combined application of SM + GM can be considered as an efficient N-fertilisation strategy for organic crops of common bean, benefiting their nutritional value without compromising yield.

Legume-Based Mobile Green Manure Can Increase Soil Nitrogen Availability and Yield of Organic Greenhouse Tomatoes.

Information about the availability of soil mineral nitrogen (N) in organic greenhouse tomatoes after the application of mobile green manure (MGM), and its impact on plant nutrient status and yield is scarce. Considering this knowledge gap, the effects of legume biomass from faba beans that are cultivated outdoors (FAB), or from feed-grade alfalfa pellets at two different doses (AAL = 330 g m-2; AAH = 660 g m-2) that were applied as MGM on the nutrition and yield of an organic greenhouse crop of tomatoes were evaluated. All of the MGM treatments increased the mineral N concentrations in the soil throughout the cropping period, and the total N concentration in tomato leaves when compared to the untreated control. FAB and AAH treatments had a stronger impact than AAL in all of the measured parameters. In addition, AAL, AAH, and FAB treatments increased the yield compared to the control by 19%, 33%, and 36%, respectively. The application of MGM, either as faba bean fresh biomass or as alfalfa dry pellets, in organic greenhouse tomatoes significantly increased the plant available soil N, improved N nutrition, and enhanced the fruit yield. However, the N mineralization rates after the MGM application were excessive during the initial cropping stages, followed by a marked decrease thereafter. This may impose an N deficiency during the late cropping period.

Impact of Plant Growth-Promoting Rhizobacteria Inoculation and Grafting on Tolerance of Tomato to Combined Water and Nutrient Stress Assessed via Metabolomics Analysis.

In the current study, inoculation with plant growth-promoting rhizobacteria (PGPR) and grafting were tested as possible cultural practices that may enhance resilience of tomato to stress induced by combined water and nutrient shortage. The roots of tomato grown on perlite were either inoculated or not with PGPR, applying four different treatments. These were PGPR-T1, a mix of two Enterobacter sp. strains (C1.2 and C1.5); PGPR-T2, Paenibacillus sp. strain DN1.2; PGPR-T3, Enterobacter mori strain C3.1; and PGPR-T4, Lelliottia sp. strain D2.4. PGPR-treated plants were either self-grafted or grafted onto Solanum lycopersicum cv. M82 and received either full or 50% of their standard water, nitrogen, and phosphorus needs. The vegetative biomass of plants subjected to PGPR-T1 was not reduced when plants were cultivated under combined stress, while it was reduced by stress to the rest of the PGPR treatments. However, PGPR-T3 increased considerably plant biomass of non-stressed tomato plants than did all other treatments. PGPR application had no impact on fruit biomass, while grafting onto 'M82' increased fruit production than did self-grafting. Metabolomics analysis in tomato leaves revealed that combined stress affects several metabolites, most of them already described as stress-related, including trehalose, myo-inositol, and monopalmitin. PGPR inoculation with E. mori strain C3.1 affected metabolites, which are important for plant/microbe symbiosis (myo-inositol and monopalmitin). The rootstock M82 did not affect many metabolites in plant leaves, but it clearly decreased the levels of malate and D-fructose and imposed an accumulation of oleic acid. In conclusion, PGPR are capable of increasing tomato tolerance to combined stress. However, further research is required to evaluate more strains and refine protocols for their application. Metabolites that were discovered as biomarkers could be used to accelerate the screening process for traits such as stress tolerance to abiotic and/or abiotic stresses. Finally, 'M82' is a suitable rootstock for tomato, as it is capable of increasing fruit biomass production.

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece.

Phaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

Impact of Legumes as a Pre-Crop on Nitrogen Nutrition and Yield in Organic Greenhouse Tomato.

An organic greenhouse crop of tomato was established in February following cultivation of cowpea (CP) or common bean (CB) for green pod production, or faba bean (FB) for green manuring. The vegetative residues of CP and CB were incorporated to the soil together with farmyard manure (FYM), prior to establishing the tomato crop. The FB plants were incorporated to the soil at anthesis together with either FYM or composted olive-mill waste (CO). Green manuring with FB resulted in higher soil mineral N levels during the subsequent tomato crop and higher tomato fruit yield when combined with FYM, compared to compost. The level of soil mineral N was the main restrictive factor for yield in organic greenhouse tomato. FB for green manuring as preceding crop to tomato increased significantly the level of soil mineral N and tomato yield compared to CB or CP aiming to produce green pods. The lowest tomato yield was obtained when the preceding crop was CB cultivated for green pod production. The soil mineral N was significantly higher when FYM was applied as base dressing compared with CO, despite the higher total N concentration in CO, pointing to slower mineralization rates of CO during tomato cultivation.

Random Material Property Fields of 3D Concrete Microstructures Based on CT Image Reconstruction.

The purpose of this paper is to determine the random spatially varying elastic properties of concrete at various scales taking into account its highly heterogeneous microstructure. The reconstruction of concrete microstructure is based on computed tomography (CT) images of a cubic concrete specimen. The variability of the local volume fraction of the constituents (pores, cement paste and aggregates) is quantified and mesoscale random fields of the elasticity tensor are computed from a number of statistical volume elements obtained by applying the moving window method on the specimen along with computational homogenization. Based on the statistical characteristics of the mesoscale random fields, it is possible to assess the effect of randomness in microstructure on the mechanical behavior of concrete.

Responses of sweet pepper (Capsicum annum L.) cultivated in a closed hydroponic system to variable calcium concentrations in the nutrient solution.

BACKGROUND: The use of water containing calcium bicarbonate (Ca(HCO3 )2 ) at excessively high concentrations in closed hydroponic crops can cause calcium ion (Ca2+ ) accumulation in the recycled nutrient solution (NS) and concomitantly negatively affect yield and product quality. The aim of the study was to determine maximum Ca2+ concentrations that do not harm the crop and to simulate the pattern of Ca2+ accumulation when the Ca2+ concentration in the irrigation water, and concomitantly in the replenishment nutrient solution (RNS), is excessive. In the current study, irrigation water containing 1.5, 3.0, 4.5 and 6.0 mmol L-1 Ca2+ was used to prepare the RNS supplied to pepper cultivated in a closed hydroponic system. RESULTS: At 1.5 mmol L-1 Ca2+ , no Ca2+ accumulation was observed in the recirculating NS. However, at 3.0, 4.5 and 6.0 mmol L-1 in the irrigation water, the Ca2+ concentration in the recirculating NS, increased by the latter cropping stages to 17, 28 and 37 mmol L-1 , corresponding to 6.4, 9.0 and 10.8 dS m-1 . The accumulation of Ca2+ in the recirculating NS affected both tissue nutrient concentrations and uptake concentrations of Ca2+ , sulphate ion (SO4 2- ) and magnesium ion (Mg2+ ), but this was not the case for nitrogen (N) or potassium ion (K+ ). Growth, yield and plant water uptake were restricted at moderate (3.0 and 4.5 mmol L-1 ) and high (6.0 mmol L-1 ) external Ca2+ levels. CONCLUSION: In soilless pepper crops with zero discharge of fertigation effluents, the Ca2+ concentration in the irrigation water and the RNS should be lower than 3.0 mmol L-1 to avoid yield restrictions due to salinity. © 2021 Society of Chemical Industry.

Genetic diversity and phylogeny of indigenous rhizobia nodulating faba bean (Vicia faba L.) in Greece.

The genetic diversity and phylogeny of fast-growing rhizobia isolated from root nodules of Vicia faba grown in different geographical regions of Greece were assessed. Although Rhizobium leguminosarum sv. viciae is the most common symbiont of Vicia spp. in European soils, there is no available information on native rhizobia nodulating faba bean in Greece. Seventy bacterial strains were isolated and grouped into sixteen distinct profiles based on BOX-PCR fingerprinting. The phylogenetic affiliation was further defined by sequence analysis of the rrs and multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and gyrB). Fifty-eight isolates were affiliated with recently described genospecies gsF-2, represented by R. laguerreae FB206T, whereas six isolates were closely related to gsB and two isolates might belong to gsA. Two isolates assigned to R. hidalgonense and another two non-nodulating strains could not be assigned to any validly defined species and possibly belong to a new rhizobial lineage. Interestingly, R. laguerreae strains were commonly found at all sampling sites, suggesting that they could be the main symbionts of faba beans in Greek soils. According to the phylogenies of two symbiosis-related genes (nodC and nifH), all nodulating isolates belonged to symbiovar (sv.) viciae harboring four distinct nodC gene haplotypes and they were grouped into two clades together with strains assigned to R. laguerreae and genospecies of R. leguminosarum isolated from other countries and continents. This is the first report that R. hidalgonense strains belong to sv. viciae. No correlation was observed between the nodC haplotypes, geographic origin and chromosomal background of the isolates in the study.

1H NMR metabolic profiling dataset of spiny chicory (Cichorium spinosum L.) exposed to abiotic stresses.

The data presented here were derived by 1H NMR metabolic profiling of stamnagathi (Cichorium spinosum L.) plants following treatments with different isosmotic salt solutions; eight saline nutrient solutions with two different levels of total molar concentrations, which were obtained by adding different amounts of NaCl, KCl, Na2SO4 or CaCl2 to the replenishment nutrient solution, were applied. The 1H NMR metabolite profiles of stamnagathi plants', which are included in this article, were recorded 56 days after transplanting. Since stamnagathi is a niche product combining unique taste and superior phytonutrient content (e.g. vitamins C and K1, lutein, ß-carotene, tocopherols, phenolic acids, fatty acids, minerals, and glutathione), the dataset could serve as a reference for future metabolomics studies related to the investigation of the effects of the four salinity sources on the plant's metabolism. Also, the dataset could be a valuable resource for the discovery of validated biomarkers of the plant's tolerance to salinity stress and responses to new plant protection products (e.g. bioelicitors). The dataset support the research article "Salinity source alters mineral composition and metabolism of Cichorium spinosum" authored by Ntatsi et al., (2017) [1].

Functional Quality, Mineral Composition and Biomass Production in Hydroponic Spiny Chicory (Cichorium spinosum L.) Are Modulated Interactively by Ecotype, Salinity and Nitrogen Supply.

The hydroponic cultivation of spiny chicory (Cichorium spinosum L.), also known as stamnagathi, allows the development of year-round production. In the current study, two contrasting stamnagathi ecotypes originating from a montane and a coastal-marine habitat were supplied with nutrient solution containing 4 or 16 mM total-N in combination with 0.3, 20, or 40 mM NaCl. The primary aim of the experiment was to provide insight into salinity tolerance and nutrient needs in the two ecotypes, thereby contributing to breeding of more resilient cultivars to salinity and nutrient stress. Nutritional qualities of the stamnagathi genotypes were also tested. The coastal-marine ecotype was more salt tolerant in terms of fresh shoot biomass production and contained significantly more water and macro- and micro-nutrients in the shoot per dry weight unit. The root Na+ concentration was markedly lower in the coastal-marine compared to the montane ecotype. The leaf Na+ concentration was similar in both ecotypes at external NaCl concentrations up to 20 mM, but significantly higher in the montane compared to the coastal-marine ecotype at 40 mM NaCl. However, the leaf Cl- concentration was consistently higher in the coastal-marine than in the montane ecotype within each salinity level. The marine ecotype also exhibited significantly less total phenols, carotenoids, flavonoids, and chlorophyll compared to the montane ecotype across all treatments. Integrating all findings, it appears that at moderate salinity levels (20 mM), the higher salt tolerance of the coastal-marine ecotype is associated with mechanisms mitigating Na+ and Cl- toxicity within the leaf tissues, such as salt dilution imposed through increased leaf succulence. Nevertheless, at high external NaCl levels, Na+ exclusion may also contribute to enhanced salt tolerance of stamnagathi. Both ecotypes exhibited a high N-use efficiency, as their shoot biomass was not restricted when the total-N supply varied from 16 to 4 mM. The leaf organic-N was not influenced by salinity, while the interaction ecotype × N-supply-level was insignificant, indicating that the mechanisms involved in the salt tolerance difference between the two ecotypes was not linked with N-acquisition or -assimilation within the plant. The current results indicate that both ecotypes are promising germplasm resources for future breeding programs.

Impact of Cultivar and Grafting on Nutrient and Water Uptake by Sweet Pepper (Capsicum annuum L.) Grown Hydroponically Under Mediterranean Climatic Conditions.

In closed-cycle hydroponic systems (CHS), nutrients and water should be delivered to the plants at identical ratios to those they are removed via plant uptake, to avoid their depletion or accumulation in the root zone. For a particular plant species and developmental stage, the nutrient to water uptake ratios, henceforth termed "uptake concentrations" (UC), remain relatively constant over time under similar climatic conditions. Thus, the nutrient to water uptake ratios can be used as nutrient concentrations in the nutrient solution (NS) supplied to CHS to compensate for nutrient and water uptake by plants. In the present study, mean UC of macro- and micronutrients were determined during five developmental stages in different pepper cultivars grown in a closed hydroponic system by measuring the water uptake and the nutrient removal from the recirculating NS. The experiment was conducted in a heated glasshouse located in Athens Mediterranean environment and the tested cultivars were 'Orangery,' 'Bellisa,' 'Sondela,' 'Sammy,' self-grafted and 'Sammy' grafted onto the commercial rootstock 'RS10' (Capsicum annuum). 'Sondela' exhibited significantly higher NO3-, Mg2+, Ca2+ and B UC, while Bellisa exhibited higher K UC in comparison with all other cultivars. The UC of all nutrients were similar in the grafted and the non-grafted 'Sammy' plants, which indicates that this Capsicum annum rootstock does not modify the uptake of nutrients and water by the scion. The UC of macronutrients estimated in the present study (mmol L-1) ranged from 2.4 to 3.7 for Ca, 1.0 to 1.5 for Mg, 6.2 to 9.0 for K, 11.7 to 13.7 for N, and 0.7 to 1.1 for P. The UC of N, K, Ca, and Mg were appreciably higher than the corresponding values found in Dutch tomato glasshouse, while that of P was similar in both locations during the vegetative stage and higher in the present study thereafter. The UC of Fe, Zn and B tended to decrease with time, while that of Mn increased initially and subsequently decreased slightly during the reproductive developmental stage.

Faba Bean Cultivation - Revealing Novel Managing Practices for More Sustainable and Competitive European Cropping Systems.

Faba beans are highly nutritious because of their high protein content: they are a good source of mineral nutrients, vitamins, and numerous bioactive compounds. Equally important is the contribution of faba bean in maintaining the sustainability of agricultural systems, as it is highly efficient in the symbiotic fixation of atmospheric nitrogen. This article provides an overview of factors influencing faba bean yield and quality, and addresses the main biotic and abiotic constraints. It also reviews the factors relating to the availability of genetic material and the agronomic features of faba bean production that contribute to high yield and the improvement of European cropping systems. Emphasis is to the importance of using new high-yielding cultivars that are characterized by a high protein content, low antinutritional compound content, and resistance to biotic and abiotic stresses. New cultivars should combine several of these characteristics if an increased and more stable production of faba bean in specific agroecological zones is to be achieved. Considering that climate change is also gradually affecting many European regions, it is imperative to breed elite cultivars that feature a higher abiotic-biotic stress resistance and nutritional value than currently used cultivars. Improved agronomical practices for faba bean crops, such as crop establishment and plant density, fertilization and irrigation regime, weed, pest and disease management, harvesting time, and harvesting practices are also addressed, since they play a crucial role in both the production and quality of faba bean.

Impact of nitrogen source and supply level on growth, yield and nutritional value of two contrasting ecotypes of Cichorium spinosum L. grown hydroponically.

BACKGROUND: In the present study, two contrasting stamnagathi (Cichorium spinosum L.) ecotypes originating either from a mountainous or from a seaside habitat were grown hydroponically and supplied with a nutrient solution differing in the total-N level (4 or 16 mmol L-1 ) and the N source (NH4+ -N/total-N: 0.05, 0.25 or 0.50). The aim was to search for genotypic differences in nitrogen nutrition. RESULTS: At commercial maturity, the dry weight of mountainous plants was higher than that of seaside plants. The shoot mineral concentrations were higher in seaside plants than in mountainous plants in both harvests. The leaf nitrate concentration was influenced by the levels of both total-N and NH4+ -N/total-N at both harvests, whereas plants with a seaside origin exhibited higher nitrate concentrations than those originating from a mountainous site in all total-N and NH4+ -N/total-N treatments. CONCLUSION: The two stamnagathi ecotypes differed considerably in their responses to nitrogen nutrition and tissue nitrate content. The mountainous ecotype was superior in terms of growth, tissue nitrate concentration and antioxidant capacity, whereas the seaside ecotype accumulated more nutrient microcations in leaves. A low total-N concentration (up to 4 mmol L-1 ) combined with a high NH4+ -N/total-N ratio (up to 0.05) could minimize tissue NO3- concentrations without compromising yield. © 2017 Society of Chemical Industry.