RESUMO
Group 2 innate lymphoid cells (ILC2s) are early effectors of mucosal type 2 immunity, producing cytokines such as interleukin (IL)-13 to mediate responses to helminth infection and allergen-induced inflammation. ILC2s are also present in lymph nodes (LNs) and can express molecules required for antigen presentation, but to date there are limited data on their dynamic behaviour. We used a CD2/IL-13 dual fluorescent reporter mouse for in vivo imaging of ILC2s and Th2 T cells in real time following a type 2 priming helminth infection or egg injection. After helminth challenge, we found that ILC2s were the main source of IL-13 in lymphoid organs (Peyer's patches and peripheral LNs), and were located in T cell areas. Intravital imaging demonstrated an increase in IL-13+ ILC2 size and movement following helminth infection, but reduced duration of interactions with T cells compared with those in homeostasis. In contrast, in the intestinal mucosa, we observed an increase in ILC2-T cell interactions post-infection, including some of prolonged duration, as well as increased IL-13+ ILC2 movement. These data suggest that ILC2 activation enhances cell motility, with the potential to increase the area of distribution of cytokines to optimise the early generation of type 2 responses. The prolonged ILC2 interactions with T cells within the intestinal mucosa are consistent with the conclusion that contact-based T cell activation may occur within inflamed tissues rather than lymphoid organs. Our findings have important implications for our understanding of the in vivo biology of ILC2s and the way in which these cells facilitate adaptive immune responses.
Assuntos
Enteropatias Parasitárias/imunologia , Subpopulações de Linfócitos/imunologia , Nippostrongylus , Esquistossomose mansoni/imunologia , Infecções por Strongylida/imunologia , Células Th2/imunologia , Animais , Genes Reporter , Interleucina-13/análise , Mucosa Intestinal/imunologia , Intestino Delgado/imunologia , Intestino Delgado/parasitologia , Microscopia Intravital , Contagem de Linfócitos , Subpopulações de Linfócitos/química , Camundongos , Especificidade de Órgãos , Organismos Livres de Patógenos Específicos , Células Th2/químicaRESUMO
Rapid activation of memory CD4(+) T helper 2 (TH2) cells during allergic inflammation requires their recruitment into the affected tissue. Here we demonstrate that group 2 innate lymphoid (ILC2) cells have a crucial role in memory TH2 cell responses, with targeted depletion of ILC2 cells profoundly impairing TH2 cell localization to the lungs and skin of sensitized mice after allergen re-challenge. ILC2-derived interleukin 13 (IL-13) is critical for eliciting production of the TH2 cell-attracting chemokine CCL17 by IRF4(+)CD11b(+)CD103(-) dendritic cells (DCs). Consequently, the sentinel function of DCs is contingent on ILC2 cells for the generation of an efficient memory TH2 cell response. These results elucidate a key innate mechanism in the regulation of the immune memory response to allergens.
Assuntos
Células Dendríticas/imunologia , Hipersensibilidade/imunologia , Memória Imunológica/imunologia , Ativação Linfocitária/imunologia , Linfócitos/imunologia , Células Th2/imunologia , Animais , Modelos Animais de Doenças , Citometria de Fluxo , Imunidade Inata/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Análise de Sequência com Séries de OligonucleotídeosRESUMO
Group 2 innate lymphoid cells (ILC2s) release interleukin-13 (IL-13) during protective immunity to helminth infection and detrimentally during allergy and asthma. Using two mouse models to deplete ILC2s in vivo, we demonstrate that T helper 2 (Th2) cell responses are impaired in the absence of ILC2s. We show that MHCII-expressing ILC2s interact with antigen-specific T cells to instigate a dialog in which IL-2 production from T cells promotes ILC2 proliferation and IL-13 production. Deletion of MHCII renders IL-13-expressing ILC2s incapable of efficiently inducing Nippostrongylus brasiliensis expulsion. Thus, during transition to adaptive T cell-mediated immunity, the ILC2 and T cell crosstalk contributes to their mutual maintenance, expansion and cytokine production. This interaction appears to augment dendritic-cell-induced T cell activation and identifies a previously unappreciated pathway in the regulation of type-2 immunity.
Assuntos
Comunicação Celular/imunologia , Antígenos de Histocompatibilidade Classe II/imunologia , Nippostrongylus/imunologia , Células Th2/imunologia , Animais , Apresentação de Antígeno/imunologia , Diferenciação Celular/imunologia , Proliferação de Células , Células Cultivadas , Técnicas de Cocultura , Células Dendríticas/imunologia , Antígenos de Histocompatibilidade Classe II/genética , Imunidade Celular , Imunidade Inata , Interleucina-13/biossíntese , Interleucina-13/metabolismo , Interleucina-2/biossíntese , Ativação Linfocitária/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos KnockoutRESUMO
Induction of Bcl6 (B cell lymphoma 6) is essential for T follicular helper (Tfh) cell differentiation of antigen-stimulated CD4(+) T cells. Intriguingly, we found that Bcl6 was also highly and transiently expressed during the CD4(+)CD8(+) (double positive [DP]) stage of T cell development, in association with the E3 ligase cullin 3 (Cul3), a novel binding partner of Bcl6 which ubiquitinates histone proteins. DP stage-specific deletion of the E3 ligase Cul3, or of Bcl6, induced the derepression of the Bcl6 target genes Batf (basic leucine zipper transcription factor, ATF-like) and Bcl6, in part through epigenetic modifications of CD4(+) single-positive thymocytes. Although they maintained an apparently normal phenotype after emigration, they expressed increased amounts of Batf and Bcl6 at basal state and produced explosive and prolonged Tfh responses upon subsequent antigen encounter. Ablation of Cul3 in mature CD4(+) splenocytes also resulted in dramatically exaggerated Tfh responses. Thus, although previous studies have emphasized the essential role of Bcl6 in inducing Tfh responses, our findings reveal that Bcl6-Cul3 complexes also provide essential negative feedback regulation during both thymocyte development and T cell activation to restrain excessive Tfh responses.
Assuntos
Diferenciação Celular/imunologia , Proteínas Culina/imunologia , Proteínas Proto-Oncogênicas c-bcl-6/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Animais , Fatores de Transcrição de Zíper de Leucina Básica/genética , Fatores de Transcrição de Zíper de Leucina Básica/imunologia , Fatores de Transcrição de Zíper de Leucina Básica/metabolismo , Western Blotting , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/metabolismo , Diferenciação Celular/genética , Proteínas Culina/genética , Proteínas Culina/metabolismo , Retroalimentação Fisiológica , Células HeLa , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Camundongos Knockout , Camundongos Transgênicos , Análise de Sequência com Séries de Oligonucleotídeos , Regiões Promotoras Genéticas/genética , Regiões Promotoras Genéticas/imunologia , Ligação Proteica/imunologia , Proteínas Proto-Oncogênicas c-bcl-6/genética , Proteínas Proto-Oncogênicas c-bcl-6/metabolismo , Receptores de Antígenos de Linfócitos T/genética , Receptores de Antígenos de Linfócitos T/imunologia , Receptores de Antígenos de Linfócitos T/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Baço/citologia , Baço/imunologia , Baço/metabolismo , Linfócitos T Auxiliares-Indutores/metabolismo , Timócitos/imunologia , Timócitos/metabolismo , Transcriptoma/imunologiaRESUMO
Type 2 innate lymphoid cells (ILC2s, nuocytes, NHC) require RORA and GATA3 for their development. We show that human ILC2s express skin homing receptors and infiltrate the skin after allergen challenge, where they produce the type 2 cytokines IL-5 and IL-13. Skin-derived ILC2s express the IL-33 receptor ST2, which is up-regulated during activation, and are enriched in lesional skin biopsies from atopic patients. Signaling via IL-33 induces type 2 cytokine and amphiregulin expression, and increases ILC2 migration. Furthermore, we demonstrate that E-cadherin ligation on human ILC2 dramatically inhibits IL-5 and IL-13 production. Interestingly, down-regulation of E-cadherin is characteristic of filaggrin insufficiency, a cardinal feature of atopic dermatitis (AD). ILC2 may contribute to increases in type 2 cytokine production in the absence of the suppressive E-cadherin ligation through this novel mechanism of barrier sensing. Using Rag1(-/-) and RORα-deficient mice, we confirm that ILC2s are present in mouse skin and promote AD-like inflammation. IL-25 and IL-33 are the predominant ILC2-inducing cytokines in this model. The presence of ILC2s in skin, and their production of type 2 cytokines in response to IL-33, identifies a role for ILC2s in the pathogenesis of cutaneous atopic disease.
Assuntos
Dermatite Atópica/metabolismo , Interleucina-17/metabolismo , Interleucinas/metabolismo , Linfócitos/citologia , Alérgenos/química , Animais , Caderinas/metabolismo , Diferenciação Celular , Separação Celular , Citocinas/metabolismo , Dermatite Atópica/imunologia , Proteínas Filagrinas , Citometria de Fluxo , Humanos , Inflamação , Interleucina-13/metabolismo , Interleucina-33 , Interleucina-5/metabolismo , Proteínas de Filamentos Intermediários/metabolismo , Leucócitos/citologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Fenótipo , Pele/metabolismoRESUMO
The differentiation of several T- and B-cell effector programs in the immune system is directed by signature transcription factors that induce rapid epigenetic remodelling. Here we report that promyelocytic leukaemia zinc finger (PLZF), the BTB-zinc finger (BTB-ZF) transcription factor directing the innate-like effector program of natural killer T-cell thymocytes, is prominently associated with cullin 3 (CUL3), an E3 ubiquitin ligase previously shown to use BTB domain-containing proteins as adaptors for substrate binding. PLZF transports CUL3 to the nucleus, where the two proteins are associated within a chromatin-modifying complex. Furthermore, PLZF expression results in selective ubiquitination changes of several components of this complex. CUL3 was also found associated with the BTB-ZF transcription factor BCL6, which directs the germinal-centre B cell and follicular T-helper cell programs. Conditional CUL3 deletion in mice demonstrated an essential role for CUL3 in the development of PLZF- and BCL6-dependent lineages. We conclude that distinct lineage-specific BTB-ZF transcription factors recruit CUL3 to alter the ubiquitination pattern of their associated chromatin-modifying complex. We propose that this new function is essential to direct the differentiation of several T- and B-cell effector programs, and may also be involved in the oncogenic role of PLZF and BCL6 in leukaemias and lymphomas.
Assuntos
Linfócitos B/metabolismo , Proteínas Culina/metabolismo , Fatores de Transcrição Kruppel-Like/metabolismo , Linfócitos T/metabolismo , Dedos de Zinco , Animais , Linfócitos B/citologia , Diferenciação Celular , Linhagem Celular , Proteínas Culina/química , Proteínas Culina/genética , Proteínas de Ligação a DNA/metabolismo , Camundongos , Proteína com Dedos de Zinco da Leucemia Promielocítica , Ligação Proteica , Transporte Proteico , Proteínas Proto-Oncogênicas c-bcl-6 , Linfócitos T/citologia , UbiquitinaçãoRESUMO
Two years ago, T helper cells, including Th1, Th2 and Th17 cells, were considered to be the most significant sources of cytokine during the orchestration of immune responses in the lung. Following the discovery of innate lymphoid cells, we now know that these previously unappreciated cytokine-secreting cells, including ILC1 (IFN-γ-expressing NK cells), ILC2 (IL-5 and IL-13-expressing nuocytes) and RORγ ILC (IL-17 and IL-22-expressing 'ILC3') are important mediators in immune processes. Herein we review the role of ILC2 specifically in inflammatory lung responses with special attention to allergen-induced and viral-induced type 2 lung disease.
Assuntos
Asma/imunologia , Hipersensibilidade/imunologia , Linfócitos/fisiologia , Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares/imunologia , Humanos , Interleucina-13/metabolismo , Interleucina-5/metabolismo , Células Th1/fisiologia , Células Th17/fisiologia , Células Th2/fisiologiaRESUMO
Airborne exposure to microbial cell wall lipids such as lipopolysaccharide triggers innate immune responses that regulate susceptibility to allergic airway inflammation. α-Glycosylceramides represent another widespread class of microbial lipids that directly stimulate innate-like, IL-4- and IL-13-producing, CD1d-restricted NKT cells. In this study, we demonstrate that NKT cells constitutively accumulate and reside in the microvasculature of the mouse lung. After a single airborne exposure to lipid antigen, they promptly extravasate to orchestrate the formation of peribronchiolar and interstitial lymphohistiocytic granulomas containing numerous eosinophils. Concomitant airborne exposure to ovalbumin (OVA) induces the priming of OVA-specific Th2 cells and IgE antibodies by the same dendritic cell coexpressing CD1d and MHC class II. Although NKT cell activation remains confined to the lipid-exposed lung and draining lymph nodes, Th2 cells recirculate and seed the lung of a parabiotic partner, conferring susceptibility to OVA challenge months after the initial exposure, in a manner independent of NKT cells and CD1d. Thus, transient recruitment and activation of lung-resident intravascular NKT cells can trigger long-term susceptibility to allergic airway inflammation.
Assuntos
Antígenos/imunologia , Hiper-Reatividade Brônquica/imunologia , Lipídeos/imunologia , Células T Matadoras Naturais/imunologia , Pneumonia/imunologia , Administração por Inalação , Animais , Células Apresentadoras de Antígenos/imunologia , Antígenos/administração & dosagem , Antígenos CD1d/imunologia , Líquido da Lavagem Broncoalveolar/citologia , Quimiocinas/genética , Quimiocinas/imunologia , Células Dendríticas/imunologia , Genes MHC da Classe II , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Ovalbumina/imunologiaRESUMO
Innate-like NKT cells conspicuously accumulate within the liver microvasculature of healthy mice, crawling on the luminal side of endothelial cells, but their general recirculation pattern and the mechanism of their intravascular behavior have not been elucidated. Using parabiotic mice, we demonstrated that, despite their intravascular location, most liver NKT cells failed to recirculate. Antibody blocking experiments established that they were retained locally through constitutive LFA-1-intercellular adhesion molecule (ICAM) 1 interactions. This unprecedented lifelong intravascular residence could be induced in conventional CD4 T cells by the sole expression of promyelocytic leukemia zinc finger (PLZF), a transcription factor specifically expressed in the NKT lineage. These findings reveal the unique genetic and biochemical pathway that underlies the innate intravascular surveillance program of NKT cells.
Assuntos
Regulação da Expressão Gênica , Molécula 1 de Adesão Intercelular/metabolismo , Fatores de Transcrição Kruppel-Like/metabolismo , Fígado/irrigação sanguínea , Antígeno-1 Associado à Função Linfocitária/metabolismo , Células T Matadoras Naturais/metabolismo , Animais , Linfócitos T CD4-Positivos/citologia , Adesão Celular , Linhagem da Célula , Citometria de Fluxo/métodos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Microcirculação , Microscopia de Fluorescência/métodos , Proteína com Dedos de Zinco da Leucemia PromielocíticaRESUMO
Humans with primary biliary cirrhosis (PBC), a disease characterized by the destruction of small bile ducts, exhibit signature autoantibodies against mitochondrial Pyruvate Dehydrogenase Complex E2 (PDC-E2) that crossreact onto the homologous enzyme of Novosphingobium aromaticivorans, an ubiquitous alphaproteobacterium. Here, we show that infection of mice with N. aromaticivorans induced signature antibodies against microbial PDC-E2 and its mitochondrial counterpart but also triggered chronic T cell-mediated autoimmunity against small bile ducts. Disease induction required NKT cells, which specifically respond to N. aromaticivorans cell wall alpha-glycuronosylceramides presented by CD1d molecules. Combined with the natural liver tropism of NKT cells, the accumulation of N. aromaticivorans in the liver likely explains the liver specificity of destructive responses. Once established, liver disease could be adoptively transferred by T cells independently of NKT cells and microbes, illustrating the importance of early microbial activation of NKT cells in the initiation of autonomous, organ-specific autoimmunity.
Assuntos
Infecções por Bactérias Gram-Negativas/imunologia , Células Matadoras Naturais/imunologia , Cirrose Hepática Biliar/imunologia , Cirrose Hepática Biliar/microbiologia , Sphingomonadaceae/imunologia , Subpopulações de Linfócitos T/imunologia , Animais , Anticorpos Antibacterianos/imunologia , Antígenos CD1/imunologia , Antígenos CD1d , Autoanticorpos/imunologia , Di-Hidrolipoil-Lisina-Resíduo Acetiltransferase/imunologia , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Hepatite Autoimune/imunologia , Hepatite Autoimune/microbiologia , Imunoglobulina A/imunologia , Imunoglobulina G/imunologia , Fígado/imunologia , Camundongos , Camundongos Endogâmicos , Proteínas Mitocondriais/imunologia , Linfócitos T/imunologiaRESUMO
The innate immune molecule surfactant protein-D (SP-D) plays an important regulatory role in the allergic airway response. In this study, we demonstrate that mice sensitized and challenged with either Aspergillus fumigatus (Af) or OVA have increased SP-D levels in their lung. SP-D mRNA and protein levels in the lung also increased in response to either rIL-4 or rIL-13 treatment. Type II alveolar epithelial cell expression of IL-4Rs in mice sensitized and challenged with Af, and in vitro induction of SP-D mRNA and protein by IL-4 and IL-13, but not IFN-gamma, suggested a direct role of IL-4R-mediated events. The regulatory function of IL-4 and IL-13 was further supported in STAT-6-deficient mice as well as in IL-4/IL-13 double knockout mice that failed to increase SP-D production upon allergen challenge. Interestingly, addition of rSP-D significantly inhibited Af-driven Th2 cell activation in vitro whereas mice lacking SP-D had increased numbers of CD4(+) cells with elevated IL-13 and thymus- and activation-regulated chemokine levels in the lung and showed exaggerated production of IgE and IgG1 following allergic sensitization. We propose that allergen exposure induces elevation in SP-D protein levels in an IL-4/IL-13-dependent manner, which in turn, prevents further activation of sensitized T cells. This negative feedback regulatory circuit could be essential in protecting the airways from inflammatory damage after allergen inhalation.
Assuntos
Bronquiolite/imunologia , Retroalimentação Fisiológica/efeitos dos fármacos , Hipersensibilidade/imunologia , Hipersensibilidade/metabolismo , Interleucina-13/farmacologia , Interleucina-4/farmacologia , Proteína D Associada a Surfactante Pulmonar/metabolismo , Alérgenos/imunologia , Animais , Bronquiolite/metabolismo , Complexo CD3/metabolismo , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD4-Positivos/imunologia , Proliferação de Células , Células Cultivadas , Quimiocina CCL17 , Quimiocinas CC/metabolismo , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Imunoglobulina E/imunologia , Inflamação/imunologia , Inflamação/metabolismo , Interferon gama/farmacologia , Interleucina-13/metabolismo , Ativação Linfocitária , Camundongos , Camundongos Knockout , Proteína D Associada a Surfactante Pulmonar/deficiência , Proteína D Associada a Surfactante Pulmonar/genética , RNA Mensageiro/genética , Receptores de Interleucina-4/metabolismo , Proteínas Recombinantes/farmacologia , Timo/citologiaRESUMO
Surfactant protein A (SP-A), a member of the collectin family, selectively binds to Pneumocystis carinii and mediates interactions between pathogen and host alveolar macrophages in vitro. To test the hypothesis that mice lacking SP-A have delayed clearance of Pneumocystis organisms and enhanced lung injury, wild-type C57BL/6 (WT) and SP-A-deficient mice (SP-A(-/-)) with or without selective CD4(+)-T-cell depletion were intratracheally inoculated with Pneumocystis organisms. Four weeks later, CD4-depleted SP-A-deficient mice had developed a more severe Pneumocystis infection than CD4-depleted WT (P. carinii pneumonia [PCP] scores of 3 versus 2, respectively). Whereas all non-CD4-depleted WT mice were free of PCP, intact SP-A(-/-) mice also had evidence of increased organism burden. Pneumocystis infection in SP-A-deficient mice was associated histologically with enhanced peribronchial and/or perivascular cellularity (score of 4 versus 2, SP-A(-/-) versus C57BL/6 mice, respectively) and a corresponding increase in bronchoalveolar lavage (BAL) cell counts. Increases in SP-D content, gamma interferon, interleukin-4, interleukin-5, and tumor necrosis factor alpha in BAL fluid occurred but were attenuated in PCP-infected SP-A(-/-) mice compared to WT mice. There were increases in total BAL NO levels in both infected groups, but nitrite levels were higher in SP-A(-/-) mice, indicating a reduction in production of higher oxides of nitrogen that was also reflected in lower levels of 3-nitrotyrosine staining in the SP-A(-/-) group. We conclude that despite increases in inflammatory cells, SP-A-deficient mice infected with P. carinii exhibit an enhanced susceptibility to the organism and attenuated production of proinflammatory cytokines and reactive oxygen-nitrogen species. These data support the concept that SP-A is a local effector molecule in the lung host defense against P. carinii in vivo.
Assuntos
Citocinas/metabolismo , Pulmão/microbiologia , Pulmão/patologia , Pneumocystis carinii/fisiologia , Proteína A Associada a Surfactante Pulmonar/deficiência , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Tirosina/análogos & derivados , Animais , Líquido da Lavagem Broncoalveolar/química , Humanos , Inflamação/complicações , Inflamação/metabolismo , Inflamação/microbiologia , Inflamação/patologia , Pulmão/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Óxido Nítrico/metabolismo , Infecções por Pneumocystis/imunologia , Infecções por Pneumocystis/metabolismo , Infecções por Pneumocystis/microbiologia , Infecções por Pneumocystis/patologia , Proteína A Associada a Surfactante Pulmonar/genética , Proteína D Associada a Surfactante Pulmonar/metabolismo , Tirosina/metabolismoRESUMO
BACKGROUND: C57BL/6 mice have attenuated allergic airway hyperresponsiveness (AHR) when compared with Balb/c mice but the underlying mechanisms remain unclear. SP-D, an innate immune molecule with potent immunosuppressive activities may have an important modulatory role in the allergic airway response and the consequent physiological changes. We hypothesized that an elevated SP-D production is associated with the impaired ability of C57BL/6 mice to develop allergic AHR. METHODS: SP-D mRNA and protein expression was investigated during development of allergic airway changes in a model of Aspergillus fumigatus (Af)-induced allergic inflammation. To study whether strain dependency of allergic AHR is associated with different levels of SP-D in the lung, Balb/c and C57BL/6 mice were compared. RESULTS: Sensitization and exposure to Af induced significant airway inflammation in both mouse strains in comparison with naïve controls. AHR to acetylcholine however was significantly attenuated in C57BL/6 mice in spite of increased eosinophilia and serum IgE when compared with Balb/c mice (p < 0.05). Af challenge of sensitized C57BL/6 mice induced a markedly increased SP-D protein expression in the SA surfactant fraction (1,894 +/- 170% of naïve controls) that was 1.5 fold greater than the increase in Balb/c mice (1,234 +/- 121% p < 0.01). These changes were selective since levels of the hydrophobic SP-B and SP-C and the hydrophilic SP-A were significantly decreased following sensitization and challenge with Af in both strains. Further, sensitized and exposed C57BL/6 mice had significantly lower IL-4 and IL-5 in the BAL fluid than that of Balb/c mice (p < 0.05). CONCLUSIONS: These results suggest that enhanced SP-D production in the lung of C57BL/6 mice may contribute to an attenuated AHR in response to allergic airway sensitization. SP-D may act by inhibiting synthesis of Th2 cytokines.
Assuntos
Aspergillus fumigatus/imunologia , Hiper-Reatividade Brônquica/etiologia , Hiper-Reatividade Brônquica/metabolismo , Hipersensibilidade/complicações , Hipersensibilidade/imunologia , Imunização , Proteína D Associada a Surfactante Pulmonar/biossíntese , Administração Intranasal , Alérgenos/imunologia , Animais , Aspergillus fumigatus/química , Hiper-Reatividade Brônquica/imunologia , Líquido da Lavagem Broncoalveolar/química , Extratos Celulares/administração & dosagem , Extratos Celulares/imunologia , Citocinas/análise , Suscetibilidade a Doenças , Feminino , Técnicas In Vitro , Inflamação/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Proteína D Associada a Surfactante Pulmonar/genética , RNA Mensageiro/metabolismo , Doenças Respiratórias/imunologia , Células Th2/metabolismoRESUMO
Pulmonary surfactant dysfunction may significantly contribute to small airway obstruction during the asthmatic response, but neither its exact role nor its regulation is clear. Surfactant function and composition was studied in an Aspergillus fumigatus (Af)-induced late-phase allergic airway response in sensitized BALB/c mice. The peak of Af-induced airway hyperresponsiveness in sensitized and challenged mice 24 h after allergen provocation coincided with a significant fall in surface activity of the pulmonary surfactant. The underlying changes included time-dependent elaboration of eotaxin and IL-5 followed by eosinophil influx into the airways. The height of airway inflammation and hyperresponsiveness was preceded by release of IL-4 and marked reductions in surfactant protein (SP)-B, a hydrophobic surfactant protein responsible for maintaining low surface tension of the lining fluid of distal air spaces. Furthermore, intratracheal administration of IL-4 significantly inhibited SP-B, indicating a regulatory role of this cytokine in the surfactant biophysical changes. Thus surfactant dysfunction induced by an IL-4-driven SP-B deficiency after allergen provocation may be an important part of the late asthmatic airway response.