RESUMO
Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV) belong to the Benyvirus genus. BSBMV has been reported only in the United States, while BNYVV has a worldwide distribution. Both viruses are vectored by Polymyxa betae and possess similar host ranges, particle number and morphology. BNYVV and BSBMV are not serologically related but they have similar genomic organizations. Field isolates usually consist of four RNA species but some BNYVV isolates contain a fifth RNA. RNAs 1 and 2 are essential for infection and replication while RNAs 3 and 4 play important roles in plant and vector interactions, respectively. Nucleotide and amino acid analyses revealed that BSBMV and BNYVV are sufficiently different to be classified as two species. Complementary base changes found within the BSBMV RNA-3 5' UTR made it resemble to BNYVV 5' RNA-3 structure whereas the 3' UTRs of both species were more conserved. cDNA clones were obtained, and allowed complete copies of BSBMV RNA-3 to be trans-replicated, trans-encapsidated by the BNYVV viral machinery. Long-distance movement was observed indicating that BSBMV RNA-3 could substitute BNYVV RNA-3 for systemic spread, even though the p29 encoded by BSBMV RNA-3 is much closer to the RNA-5-encoded p26 than to BNYVV RNA-3-encoded p25. Competition occurred when BSBMV RNA-3-derived replicons were used together with BNYVV-derived RNA-3 but not when the RNA-5-derived component was used. Exploitation of the similarities and divergences between BSBMV and BNYVV should lead to a better understanding of molecular interactions between Benyviruses and their hosts.
Assuntos
Amaranthaceae/virologia , Doenças das Plantas/virologia , Vírus de Plantas/fisiologia , Vírus de RNA/fisiologia , Proteínas Virais/metabolismo , Sequência de Bases , Regulação Viral da Expressão Gênica , Dados de Sequência Molecular , Vírus de Plantas/genética , Vírus de RNA/química , Vírus de RNA/genética , RNA Viral/genética , RNA Viral/metabolismo , Proteínas Virais/químicaRESUMO
The p25 protein encoded by Beet necrotic yellow vein virus (BNYVV) RNA-3 is a pathogenicity determinant that has been implicated in symptom exacerbation on Chenopodiaceae hosts. Several p25 variants exist within natural isolates and p25 sequence variation may influence the degree of pathogenicity of such BNYVV isolates. Expression of p25 from natural A- and P-type isolates in the background of B-type BNYVV cDNA clones gave symptom discrepancies when compared to B-type p25 expression. Such pathogenicity fluctuation was not due to a different subcellular localization of p25 but was correlated with the nature of the tetrad motif present between amino acid residues 67-70, as well as with the capacity of p25 to self-associate and to activate transcription in a yeast one-hybrid system. Our data suggest that the complete sequence of p25 is required for its functions and the identified sequence variations may contribute to correct folding of the protein.
Assuntos
Aizoaceae/virologia , Vírus de Plantas/genética , Vírus de Plantas/patogenicidade , Vírus de RNA/genética , Vírus de RNA/patogenicidade , Proteínas Virais/genética , Genes Virais , Variação Genética , Doenças das Plantas/virologia , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Ativação Transcricional , Técnicas do Sistema de Duplo-Híbrido , Proteínas Virais/fisiologia , Virulência/genéticaRESUMO
A study of molecular diversity was carried out on 136 sugar beets infected with Beet necrotic yellow vein virus (BNYVV, Benyvirus) collected worldwide. The nucleotide sequences of the RNA-2-encoded CP, RNA-3-encoded p25 and RNA-5-encoded p26 proteins were analysed. The resulting phylogenetic trees allowed BNYVV to be classified into groups that show correlations between the virus clusters and geographic origins. The selective constraints on these three sequences were measured by estimating the ratio between synonymous and non-synonymous substitution rates (omega) with maximum-likelihood models. The results suggest that selective constraints are exerted differently on the proteins. CP was the most conserved, with mean omega values ranging from 0.12 to 0.15, while p26 was less constrained, with mean omega values ranging from 0.20 to 0.33. Selection was detected in three amino acid positions of p26, with omega values of about 5.0. The p25 sequences presented the highest mean omega values (0.36-1.10), with strong positive selection (omega=4.7-54.7) acting on 14 amino acids, and particularly on amino acid 68, where the omega value was the highest so far encountered in plant viruses.
Assuntos
Beta vulgaris/virologia , Variação Genética , Filogenia , Vírus de RNA/classificação , Proteínas do Capsídeo/genética , Vírus de Plantas/genética , Vírus de RNA/genética , RNA Viral/análise , Homologia de Sequência do Ácido NucleicoRESUMO
A Beet necrotic yellow vein virus isolate containing a fifth RNA is present in the Pithiviers area of France. A full-length cDNA clone of RNA-5 was obtained and placed under the control of a T(7)-RNA-pol promoter that allowed the production of infectious transcripts. "Pithiviers" isolate-specific necrotic symptoms were obtained on Chenopodium quinoa when RNA-5-encoded p26 was expressed either from RNA-5 or from an RNA-3-derived replicon. By using haemagglutinin- and green fluorescent protein-tagged constructs, virally expressed p26-fusion proteins induced the same necrotic local lesions on host plants and were localized mainly in the nucleus of infected cells. Deletion mutagenesis permitted identification of two domains, responsible respectively for nuclear export and cytoplasmic retention of the p26 mutated proteins. By using a yeast two-hybrid system, Gal4DB-p26 protein self-activated transcription of the His3 reporter gene. The p26 transcription-activation domain was located within its first 55 aa and has been studied by alanine scanning. Resulting p26 mutants were tested for their capability to induce necrotic symptoms and to localize in the nuclear compartment.
