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In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising "hard" and "soft" protein coronas. However, a comprehensive picture regarding the protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evolution of protein corona formation. Our results show that protein corona formation is kinetically divided into three different phases: phase 1, proteins irreversibly and directly bound (under physiologically relevant conditions) to the particle surface; phase 2, irreversibly bound proteins interacting with preadsorbed proteins, and phase 3, reversibly bound "soft" protein corona proteins. Additionally, we investigate particle-protein interactions on low-fouling zwitterionic-coated particles where the adsorption of irreversibly bound proteins does not occur, and on such particles, only a "soft" protein corona is formed. The reported approach offers the potential to define new state-of-the art procedures for kinetics and protein fouling experiments.
Assuntos
Dispositivos Lab-On-A-Chip , Nanopartículas/química , Coroa de Proteína/química , Dióxido de Silício/química , Humanos , Microfluídica , Microscopia ConfocalRESUMO
The control of the distribution of colloidal particles in microfluidic flows plays an important role in biomedical and industrial applications. A particular challenge is to induce cross-streamline migration in laminar flows, enabling the separation of colloidal particles according to their size, shape or elasticity. Here we show that viscoelastic fluids can mediate cross-streamline migration of deformable spherical and cylindrical colloidal particles in sinusoidal microchannels at low Reynolds numbers. For colloidal streams focused into the center of the channel entrance this leads to a symmetric stream-splitting and separation into four substreams. The degree of stream splitting and separation can be controlled via the flow rates, viscoelasticity of the focusing fluid, and the spatial microchannel modulation with an upper limit when reaching the microchannel walls. We demonstrate that this effect can be used to separate flexible particles of different size and shape. This methodology of cross-stream migration has thus great potential for the passive separation of colloids and cells in microfluidic channels.
Assuntos
Hidrodinâmica , Microfluídica/métodos , ColoidesRESUMO
Block copolymer self-assembly in solution paves the way for the construction of well-defined compartmentalized nanostructures. These are excellent templates for the incorporation and stabilisation of nanoparticles (NPs), giving rise to highly relevant applications in the field of catalysis or sensing. However, the regio-selective incorporation of NPs in specific compartments is still an issue, especially concerning the loading with different NP types. Using crystallisation-driven self-assembly (CDSA), functional worm-like crystalline-core micelles (wCCMs) with a tailor-made, nanometre-sized patchy corona were prepared as versatile templates for the incorporation and stabilisation of metal and metal oxide NPs. Different strategies, like ligand exchange or co-precipitation of polymer stabilised NPs with one surface patch, were developed that allow the incorporation of NPs in specific regions of the patchy wCCM corona. Independent of the NP type and the incorporation method, the NPs showed no tendency for agglomeration and were fixed within the corona patches of the wCCMs. The binary loading of patchy micelles with metal and metal oxide NPs was realised by combining different loading strategies, yielding hybrids with homogeneously dispersed NPs guided by the patchy structure of the template.
RESUMO
Liquid microjets play a key role in fiber spinning, inkjet printing, and coating processes. In all of these applications, the liquid jets carry dispersed particles whose spatial and orientational distributions within the jet critically influence the properties of the fabricated structures. Despite its importance, there is currently no knowledge about the orientational distribution of particles within microjets and droplets. Here, we demonstrate a microfluidic device that allows to determine the local particle distribution and orientation by X-ray scattering. Using this methodology, we discovered unexpected changes in the particle orientation upon exiting the nozzle to form a free jet, and upon jet break-up into droplets, causing an unusual biaxial particle orientation. We show how flow and aspect ratio determine the flow orientation of anisotropic particles. Furthermore, we demonstrate that the observed phenomena are a general characteristic of anisotropic particles. Our findings greatly enhance our understanding of particle orientation in free jets and droplets and provide a rationale for controlling particle alignment in liquid jet-based fabrication methodologies.
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We report the fabrication of highly permeable membranes in poly(ethylene glycol) diacrylate (PEGDA) channels, for investigating ultra- or micro-filtration, at the microfluidic scale. More precisely, we used a maskless UV projection setup to photo-pattern PEG-based hydrogel membranes on a large scale (mm-cm), and with a spatial resolution of a few microns. We show that these membranes can withstand trans-membrane pressure drops of up to 7 bar without any leakage, thanks to the strong anchoring of the hydrogel to the channel walls. We also report in situ measurements of the Darcy permeability of these membranes, as a function of the deposited energy during photo-polymerization, and their formulation composition. We show that the use of PEG chains as porogens, as proposed in [Lee et al., Biomacromolecules, 2010, 11, 3316], significantly increases the porosity of the hydrogels, up to Darcy permeabilities of about 1.5 × 10-16 m2, while maintaining the strong mechanical stability of the membranes. We finally illustrate the opportunities offered by this technique, by investigating frontal filtration of colloidal dispersions in a straight microfluidic channel.
RESUMO
Delamination is a key step to obtain individual layers from inorganic layered materials needed for fundamental studies and applications. For layered van der Waals materials such as graphene, the adhesion forces are small, allowing for mechanical exfoliation, whereas for ionic layered materials such as layered silicates, the energy to separate adjacent layers is considerably higher. Quite counterintuitively, we show for a synthetic layered silicate (Na0.5-hectorite) that a scalable and quantitative delamination by simple hydration is possible for high and homogeneous charge density, even for aspect ratios as large as 20000. A general requirement is the separation of adjacent layers by solvation to a distance where layer interactions become repulsive (Gouy-Chapman length). Further hydration up to 34 nm leads to the formation of a highly ordered lamellar liquid crystalline phase (Wigner crystal). Up to eight higher-order reflections indicate excellent positional order of individual layers. The Wigner crystal melts when the interlayer separation reaches the Debye length, where electrostatic interactions between adjacent layers are screened. The layers become weakly charge-correlated. This is indicated by fulfilling the classical Hansen-Verlet and Lindeman criteria for melting. We provide insight into the requirements for layer separation and controlling the layer distances for a broad range of materials and outline an important pathway for the integration of layers into devices for advanced applications.