Exploratory study evaluating the relationships between perinatal adversity, oxidative stress, and infant neurodevelopment across the first year of life.

Early childhood adversity increases risk for negative lifelong impacts on health and wellbeing. Identifying the risk factors and the associated biological adaptations early in life is critical to develop scalable early screening tools and interventions. Currently, there are limited, reliable early childhood adversity measures that can be deployed prospectively, at scale, to assess risk in pediatric settings. The goal of this two-site longitudinal study was to determine if the gold standard measure of oxidative stress, F2-Isoprostanes, is potentially a reliable measure of a physiological response to adversity of the infant and mother. The study evaluated the independent relationships between F2-Isoprostanes, perinatal adversity and infant neurocognitive development. The study included mother-infant dyads born >36 weeks' gestation. Maternal demographic information and mental health assessments were utilized to generate a perinatal cumulative risk score. Infants' development was assessed at 6 and 12 months and both mothers and infants were assayed for F2-isoprostane levels in blood and urine, respectively. Statistical analysis revealed that cumulative risk scores correlated with higher maternal (p = 0.01) and infant (p = 0.05) F2-isoprostane levels at 6 months. Infant F2-isoprostane measures at 2 months were negatively associated with Mullen Scales of Early Learning Composite scores at 12 months (p = 0.04). Lastly, higher cumulative risk scores predicted higher average maternal F2-isoprostane levels across the 1-year study time period (p = 0.04). The relationship between perinatal cumulative risk scores and higher maternal and infant F2-isoprostanes at 6 months may reflect an oxidative stress status that informs a sensitive period in which a biomarker can be utilized prospectively to reveal the physiological impact of early adversity.

Improving Children's Expressive Language and Auditory Comprehension Through Responsive Caregiving: Evidence from a Randomized Controlled Trial of a Strength-Based Video-Coaching Intervention.

Young children from low-SES backgrounds are at higher risk for delayed language development, likely due to differences in their home language environment and decreased opportunities for back and forth communicative exchange. Intervention strategies that encourage reciprocal caregiver-child interactions may effectively promote young children's language development and enhance optimal language outcomes. The Filming Interactions to Nurture Development (FIND) program is a brief strength-based video-coaching intervention designed to promote increased back and forth ("serve and return") interactions between caregivers and their children. The current study used data from a randomized controlled trial (RCT) to examine the effectiveness of the FIND program in improving auditory comprehension and expressive communication skills among children from low-SES backgrounds. The current study used a pretest-posttest design to evaluate intervention effects from an RCT with 91 low-SES families. Families with children aged 4 to 36 months old (41.8% female) were randomly assigned to an active control or FIND intervention group. Children's auditory comprehension and expressive communication were assessed using the Preschool Language Scales, Fifth Edition (PLS-5) during both pre- and post-intervention sessions. Children in the FIND intervention group showed significantly increased expressive communication skills and a non-significant increase in auditory comprehension skills across the intervention period. In contrast, children in the active control group showed non-significant changes in expressive communication and a statistically significant decline in auditory comprehension abilities between pre- and post-intervention assessments. All analyses controlled for sex, age, and home language. This study provides preliminary evidence that the FIND intervention promotes the development of expressive and receptive language skills among young children in high-stress, low-SES environments.

Maternal stress and development of infant attention to threat-related facial expressions.

Attentional biases to threat-related stimuli, such as fearful and angry facial expressions, are important to survival and emerge early in development. Infants demonstrate an attentional bias to fearful facial expressions by 5-7 months of age and an attentional bias toward anger by 3 years of age that are modulated by experiential factors. In a longitudinal study of 87 mother-infant dyads from families predominantly experiencing low income, we examined whether maternal stress and depressive symptoms were associated with trajectories of attentional biases to threat, assessed during an attention disengagement eye-tracking task when infants were 6-, 9-, and 12-month old. By 9 months, infants demonstrated a generalized bias toward threat (both fearful and angry facial expressions). Maternal perceived stress was associated with the trajectory of the bias toward angry facial expressions between 6 and 12 months. Specifically, infants of mothers with higher perceived stress exhibited a greater bias toward angry facial expressions at 6 months that decreased across the next 6 months, compared to infants of mothers with lower perceived stress who displayed an increased bias to angry facial expressions over this age range. Maternal depressive symptoms and stressful life events were not associated with trajectories of infant attentional bias to anger or fear. These findings highlight the role of maternal perceptions of stress in shaping developmental trajectories of threat-alerting systems.

Suspension and expulsion in Colorado early care and education settings: Child, program, and community-level predictors.

Expulsion presents a significant risk in Early Care and Education (ECE) settings due to its association with early disadvantage and frequency of use. A statewide survey was conducted to characterize ECE suspension and expulsion (S/E) in Colorado. Child, program, and community-level factors were examined as predictors of S/E, alongside preventative intervention supports capable of reducing S/E. Six hundred and sixty three licensed child care programs participated in the survey, providing information on 19,848 enrolled children, 312 suspensions, and 74 expulsions. A series of multilevel hierarchical regressions were conducted, examining predictors for S/E. Children with IEPs/IFSPs were disproportionately suspended; 2-3-year-olds were disproportionately expelled; and boys, 5-6-year-olds, and children with disabilities were disproportionately suspended and expelled. Center-based programs, lower quality ratings, and "zero tolerance" policies predicted greater use of suspension. In contrast, school-district affiliation and knowledge of how to access infant and early childhood mental health consultation (IECMHC) decreased suspension. Lower levels of regional child poverty predicted higher use of expulsion. Affiliation with a school-district, IECMH consultant-led training, and use of quality coaches predicted decreased expulsion. This study identifies the need for ongoing protection for children with IEPs/ IFSPs and the preventative interventions that mitigate risk for S/E.

Introducción. La expulsión presenta un riesgo significativo en el marco del Temprano Cuidado y Educación (ECE) debido a su asociación con tempranas desventajas y la frecuencia de uso. Una encuesta a nivel estatal se llevó a cabo para caracterizar la suspensión y expulsión de ECE (S/E) en Colorado. Asuntos relacionados con el niño, el programa y de nivel comunitario se examinaron como factores de predicción de (S/E), junto con apoyos de intervención para prevenir, capaces de reducir S/E. Métodos. Participaron en la encuesta 663 programas de cuidado infantil autorizados, lo cual aportó información sobre 19,848 niños matriculados, 312 suspensiones y 74 expulsiones. Se llevó a cabo una serie de regresiones jerárquicas de niveles múltiples, examinando así los factores de predicción de S/E. Resultados. A los niños con participación en programas o planes IEP/IFSP se les suspendió de manera desproporcionada; niños entre los dos y tres años fueron desproporcionalmente expulsados; y varones de cinco a seis años, y niños con discapacidades fueron desproporcionalmente suspendidos y expulsados. Los programas con base en un centro, los más bajos puntajes de evaluación de la calidad y las políticas de 'tolerancia cero' predijeron el mayor uso de la suspensión. En contraste, la afiliación a un distrito escolar y el conocimiento de cómo tener acceso a la consulta de salud mental infantil y la temprana niñez (IECMHC) redujo las suspensiones. Los más bajos niveles de pobreza regional de niños predijeron el más alto uso de la expulsión. La afiliación a un distrito escolar, el entrenamiento de consultores de IECMH, así como el uso de entrenadores calificados predijeron una reducción de las expulsiones. Conclusiones. Este estudio identifica la necesidad de una continuada protección para niños en programas o planes IEP/IFSP y las intervenciones de prevención que mitigan el riesgo de S/E.

INTRODUCTION: L'expulsion présente un risque important pour les contextes de Soins Précoces et Education (en anglais Early Care and Education, abrégé ici ECE) du fait de son lien à un désavantage précoce et la fréquence de l'utilisation. Un sondage au niveau de l'état a été fait afin de caractériser la suspension ECE et l'expulsion (S/E) dans l'état du Colorado aux Etats-Unis. L'enfant, le programme et les facteurs aux niveau de la communauté ont été examinés en tant que facteurs de prédiction de la (S/E), en parallèle avec des soutiens d'une intervention préventive capable de réduire la S/E. Méthodes. 663 programmes de crèches accréditées ont participé au questionnaire, offrant des renseignements sur 19848 enfants inscrits, 312 suspensions et 74 expulsions. Une série de régressions hiérarchiques à niveaux multiples a été faite, examinant les facteurs de prédiction pour la S/E. Résultats. Les enfants avec des plans IEP/IFSP (Plan d'Enseignement Individualisé/Plan Individualisé de Service à la Famille) ont été suspendus de manière disproportionnée ; les enfants de deux à trois ans ont été expulsés de manière disproportionnée, et les enfants avec des handicaps ont été suspendus et expulsée de manière disproportionnée. Les programmes centres (centrés sur une condition), les cotes de qualité inférieure et les politiques de « zéro tolérance ¼ ont prédit une plus grande utilisation de la suspension. Par contre, l'affiliation à un district académique et des connaissances sur la manière d'accéder à la consultation de santé mentale du nourrisson et de la petite enfance (IECMHC) ont fait décroître le taux de suspension. Des niveaux plus bas de pauvreté de l'enfant régionale ont prédit une utilisation plus élevée de la suspension. L'affiliation à un district académique, la formation faite par un consultant IECMH et l'utilisation de formateurs de qualité ont prédit une expulsion moins élevée. Cette étude identifie le besoin de protection continue des enfants avec des plans IEP/IFSP et d'interventions préventives qui mitigent le risque de S/E.

Parental buffering in the context of poverty: positive parenting behaviors differentiate young children's stress reactivity profiles.

Experiencing poverty increases vulnerability for dysregulated hypothalamic-pituitary-adrenal (HPA) axis functioning and compromises long-term health. Positive parenting buffers children from HPA axis reactivity, yet this has primarily been documented among families not experiencing poverty. We tested the theorized power of positive parenting in 124 parent-child dyads recruited from Early Head Start (Mage = 25.21 months) by examining child cortisol trajectories using five samples collected across a standardized stress paradigm. Piecewise latent growth models revealed that positive parenting buffered children's stress responses when controlling for time of day, last stress task completed, and demographics. Positive parenting also interacted with income such that positive parenting was especially protective for cortisol reactivity in families experiencing greater poverty. Findings suggest that positive parenting behaviors are important for protecting children in families experiencing low income from heightened or prolonged physiologic stress reactivity to an acute stressor.

Association of Perceived Maternal Stress During the Perinatal Period With Electroencephalography Patterns in 2-Month-Old Infants.

Importance: Variation in child responses to adversity creates a clinical challenge to identify children most resilient or susceptible to later risk for disturbances in cognition and health. Advances in establishing scalable biomarkers can lead to early identification and mechanistic understanding of the association of early adversity with neurodevelopment. Objectives: To examine whether maternal reports of stress are associated with patterns in resting electroencephalography at 2 months of age and whether unique electroencephalographic profiles associated with risk and resiliency factors can be identified. Design, Setting, and Participants: For this cohort study, a population-based sample of 113 mother-infant dyads was recruited from January 1, 2016, to March 1, 2018, during regularly scheduled pediatric visits before infants were 2 months of age from 2 primary care clinics in Boston, Massachusetts, and Los Angeles, California, that predominantly serve families from low-income backgrounds. Data are reported from a single time point, when infants were aged 2 months, of an ongoing cohort study longitudinally following the mother-infant dyads. Exposures: Maternal reported exposure to stressful life events and perceived stress. Main Outcomes and Measures: Spectral power (absolute and relative) in different frequency bands (Δ, θ, low and high α, ß, and γ) from infant resting electroencephalography (EEG) and EEG profiles across frequency bands determined by latent profile analysis. Results: Of 113 enrolled infants, 70 (mean [SD] age, 2.42 [0.37] months; 35 girls [50%]) provided usable EEG data. In multivariable hierarchical linear regressions, maternal perceived stress was significantly and negatively associated with absolute ß (ß = -0.007; 95% CI, -0.01 to -0.001; semipartial r = -0.25) and γ power (ß = -0.008; 95% CI, -0.01 to -0.002; semipartial r = -0.28). Maternal educational level was significantly and positively associated with power in high α, ß, and γ bands after adjusting for covariates (high school: γ: ß = 0.108; 95% CI, 0.014-0.203; semipartial r = -0.236; associate's degree or higher: high α: ß = 0.133; 95% CI, 0.018-0.248; semipartial r = 0.241; ß: ß = 0.167; 95% CI, 0.055-0.279; semipartial r = 0.309; and γ: ß = 0.183; 95% CI, 0.066-0.299; semipartial r = 0.323). Latent profile analysis identified 2 unique profiles for absolute and relative power. Maternal perceived stress (ß = 0.13; 95% CI, 0.01-0.25; adjusted odds ratio [AOR], 1.14; 95% CI, 1.01-1.28) and maternal educational level (high school: ß = 3.00; 95% CI, 0.35-5.65; AOR, 20.09; 95% CI, 1.42-283.16; associate's degree or higher: ß = 4.12; 95% CI, 1.45-6.79; AOR, 61.56; 95% CI, 4.28-885.01) were each associated with unique profile membership. Conclusions and Relevance: These findings suggest that unique contributions of caregiver stress and maternal educational level on infant neurodevelopment are detectable at 2 months; EEG might be a promising tool to identify infants most susceptible to parental stress and to reveal mechanisms by which neurodevelopment is associated with adversity. Additional studies validating subgroups across larger cohorts with different stressors and at different ages are required before use at the individual level in clinical settings.

2D Cocrystallization from H-Bonded Organic Ferroelectrics.

The synthesis of 2D H-bonded cocrystals from the room-temperature ferroelectric organics croconic acid (CA) and 3-hydroxyphenalenone (3-HPLN) is demonstrated through self-assembly on a substrate under ultrahigh vacuum. 2D cocrystal polymorphs of varied stoichiometry were identified with scanning tunneling microscopy, and one of the observed structural building blocks consists of two CA and two 3-HPLN molecules. Computational analysis with density functional theory confirmed that the experimental (CA)2(3-HPLN)2 tetramers are lower in energy than single-component structures due to the ability of the tetramers to pack efficiently in two dimensions, the promotion of favorable electrostatic interactions between tetramers, and the optimal number of intermolecular hydrogen bonds. The structures investigated, especially the experimentally found tetrameric building blocks, are not polar. However, it is demonstrated computationally that cocrystallization can, in principle, result in heterogeneous structures with dipole moments that exceed those of homogeneous structures and that 2D structures with select stoichiometries could favor metastable polar structures.

Distribution of progesterone receptor immunoreactivity in the midbrain and hindbrain of postnatal rats.

Nuclear steroid hormone receptors are powerful transcription factors and therefore have the potential to influence and regulate fundamental processes of neural development. The expression of progesterone receptors (PR) has been described in the developing forebrain of rats and mice, and the mammalian brain may be exposed to significant amounts of progesterone, either from maternal sources and/or de novo synthesis of progesterone from cholesterol within the brain. The present study examined the distribution of PR immunoreactive (PRir) cells within the midbrain and hindbrain of postnatal rats. The results demonstrate that PR is transiently expressed within the first 2 weeks of life in specific motor, sensory and reticular core nuclei as well as within midbrain dopaminergic cell groups such as the substantia nigra and the ventral tegmental area. Additionally, robust PRir was observed in cells of the lower rhombic lip, a transient structure giving rise to precerebellar nuclei. These results suggest that progestins and progesterone receptors may play a fundamental role in the postnatal development of numerous midbrain and hindbrain nuclei, including some areas implicated in human disorders. Additionally, these findings contribute to the increasing evidence that steroid hormones and their receptors influence neural development in a wide range of brain areas, including many not typically associated with reproduction or neuroendocrine function.