Polydisperse molecular architecture of connexin 26/30 heteromeric hemichannels revealed by atomic force microscopy imaging.

Connexin (Cx) protein forms hemichannels and gap junctional channels, which play diverse and profound roles in human physiology and diseases. Gap junctions are arrays of intercellular channels formed by the docking of two hemichannels from adjacent cells. Each hexameric hemichannel contains the same or different Cx isoform. Although homomeric Cxs forms have been largely described functionally and structurally, the stoichiometry and arrangement of heteromeric Cx channels remain unknown. The latter, however, are widely expressed in human tissues and variation might have important implications on channel function. Investigating properties of heteromeric Cx channels is challenging considering the high number of potential subunit arrangements and stoichiometries, even when only combining two Cx isoforms. To tackle this problem, we engineered an HA tag onto Cx26 or Cx30 subunits and imaged hemichannels that were liganded by Fab-epitope antibody fragments via atomic force microscopy. For Cx26-HA/Cx30 or Cx30-HA/Cx26 heteromeric channels, the Fab-HA binding distribution was binomial with a maximum of three Fab-HA bound. Furthermore, imaged Cx26/Cx30-HA triple liganded by Fab-HA showed multiple arrangements that can be derived from the law of total probabilities. Atomic force microscopy imaging of ringlike structures of Cx26/Cx30-HA hemichannels confirmed these findings and also detected a polydisperse distribution of stoichiometries. Our results indicate a dominant subunit stoichiometry of 3Cx26:3Cx30 with the most abundant subunit arrangement of Cx26-Cx26-Cx30-Cx26-Cx30-Cx30. To our knowledge, this is the first time that the molecular architecture of heteromeric Cx channels has been revealed, thus providing the basis to explore the functional effect of these channels in biology.

Blackberry Vinegar Produced By Successive Acetification Cycles: Production, Characterization And Bioactivity Parameters

ABSTRACT: Blackberry vinegar was produced in successive acetification cycles and content of total phenolics, anthocyanins and antioxidant activity were evaluated along the production. Firstly, blackberry wine was obtained in bench-scale bioreactor, being verified 0.39 g/g ethanol yield, 1.78 g/L.h volumetric productivity and 76% efficiency. After, three successive acetification cycles were conducted efficiently in grapia barrel with average acetic acid production of 51.6 g/L, 72.2 % acetic acid yield and 0.4 g/L.h volumetric productivity. Appreciable contents of polyphenolic compounds, anthocyanins and high antioxidant activity were observed in the raw material, wine and vinegar obtained in each cycle of acetic acid transformation. Acetic acid transformation led the small reduction of antioxidant activity compared to alcoholic fermentation, but the antioxidant potential was maintained along the cycles. The content of total phenolics and anthocyanins also suffered a reduction in step of acetification.

Concentrações de metais pesados em grãos de café produzidos em lavouras sobre solos originados do basalto e do arenito Caiuá / Heavy metals concentration in coffee grains produced in farming under basalt and Caiuá sandstone soils

Tendo em vista que o Brasil é um grande produtor e consumidor de café, fica evidente a importância de sua caracterização em diferentes locais de cultivo. O objetivo do presente estudo foi quantificar os teores de metais pesados em amostras de grãos de café, provenientes de lavouras implantadas em solos oriundos do basalto e do arenito Caiuá do Estado do Paraná. Foram examinadas 30 amostras de grãos de café, sendo 15 cultivados em solos predominantemente arenosos e 15 em solos argilosos. A determinação de Cromo (Cr), Cobalto (Co), Níquel (Ni), Chumbo (Pb), Cádmio (Cd), Cobre (Cu), Zinco (Zn) e Manganês (Mn) foi feita por espectrometria de emissão atômica com fonte de plasma induzido com Thermo Jarrel-ash ICAP 612 E (ICP-EAS). Os níveis foram variáveis entre as amostras.

Considering that Brazil is a big producer and coffee consumer its evident the importance of its characterization in the different places of cultivation. The purpose of the present study was quantify the levels of high metals in coffee grains samples 'IAPAR 59' derived from farming introduced in basalt and Caiuá sandstone soils in Paraná, Brazil . Thirty ground samples were examined, being 15 of predominantly arenaceous ground and 15 of argillaceous ground. The determination of Chrome (Cr), Cobalt (Co), Nickel (Ni), Lead (Pb), Cadmium (Cd), Copper (Cu), Zinc (Zn) and Manganese (Mn), was made through atomic emission spectrometry with induced plasma source with Thermo 612 Jarrel-ash ICAP E (ICP-EAS). The levels changed among the samples.

Concentrações de metais pesados em grãos de café produzidos em lavouras sobre solos originados do basalto e do arenito Caiuá

Considering that Brazil is a big producer and coffee consumer its evident the importance of its characterization in the different places of cultivation. The purpose of the present study was quantify the levels of high metals in coffee grains samples 'IAPAR 59' derived from farming introduced in basalt and Caiuá sandstone soils in Paraná, Brazil . Thirty ground samples were examined, being 15 of predominantly arenaceous ground and 15 of argillaceous ground. The determination of Chrome (Cr), Cobalt (Co), Nickel (Ni), Lead (Pb), Cadmium (Cd), Copper (Cu), Zinc (Zn) and Manganese (Mn), was made through atomic emission spectrometry with induced plasma source with Thermo 612 Jarrel-ash ICAP E (ICP-EAS). The levels changed among the samples.

Tendo em vista que o Brasil é um grande produtor e consumidor de café, fica evidente a importância de sua caracterização em diferentes locais de cultivo. O objetivo do presente estudo foi quantificar os teores de metais pesados em amostras de grãos de café, provenientes de lavouras implantadas em solos oriundos do basalto e do arenito Caiuá do Estado do Paraná. Foram examinadas 30 amostras de grãos de café, sendo 15 cultivados em solos predominantemente arenosos e 15 em solos argilosos. A determinação de Cromo (Cr), Cobalto (Co), Níquel (Ni), Chumbo (Pb), Cádmio (Cd), Cobre (Cu), Zinco (Zn) e Manganês (Mn) foi feita por espectrometria de emissão atômica com fonte de plasma induzido com Thermo Jarrel-ash ICAP 612 E (ICP-EAS). Os níveis foram variáveis entre as amostras.

Concentrações de metais pesados em grãos de café produzidos em lavouras sobre solos originados do basalto e do arenito Caiuá

Considering that Brazil is a big producer and coffee consumer its evident the importance of its characterization in the different places of cultivation. The purpose of the present study was quantify the levels of high metals in coffee grains samples 'IAPAR 59' derived from farming introduced in basalt and Caiuá sandstone soils in Paraná, Brazil . Thirty ground samples were examined, being 15 of predominantly arenaceous ground and 15 of argillaceous ground. The determination of Chrome (Cr), Cobalt (Co), Nickel (Ni), Lead (Pb), Cadmium (Cd), Copper (Cu), Zinc (Zn) and Manganese (Mn), was made through atomic emission spectrometry with induced plasma source with Thermo 612 Jarrel-ash ICAP E (ICP-EAS). The levels changed among the samples.

Tendo em vista que o Brasil é um grande produtor e consumidor de café, fica evidente a importância de sua caracterização em diferentes locais de cultivo. O objetivo do presente estudo foi quantificar os teores de metais pesados em amostras de grãos de café, provenientes de lavouras implantadas em solos oriundos do basalto e do arenito Caiuá do Estado do Paraná. Foram examinadas 30 amostras de grãos de café, sendo 15 cultivados em solos predominantemente arenosos e 15 em solos argilosos. A determinação de Cromo (Cr), Cobalto (Co), Níquel (Ni), Chumbo (Pb), Cádmio (Cd), Cobre (Cu), Zinco (Zn) e Manganês (Mn) foi feita por espectrometria de emissão atômica com fonte de plasma induzido com Thermo Jarrel-ash ICAP 612 E (ICP-EAS). Os níveis foram variáveis entre as amostras.

Concentrações de metais pesados em grãos de café produzidos em lavouras sobre solos originados do basalto e do arenito Caiuá

Considering that Brazil is a big producer and coffee consumer its evident the importance of its characterization in the different places of cultivation. The purpose of the present study was quantify the levels of high metals in coffee grains samples 'IAPAR 59' derived from farming introduced in basalt and Caiuá sandstone soils in Paraná, Brazil . Thirty ground samples were examined, being 15 of predominantly arenaceous ground and 15 of argillaceous ground. The determination of Chrome (Cr), Cobalt (Co), Nickel (Ni), Lead (Pb), Cadmium (Cd), Copper (Cu), Zinc (Zn) and Manganese (Mn), was made through atomic emission spectrometry with induced plasma source with Thermo 612 Jarrel-ash ICAP E (ICP-EAS). The levels changed among the samples.

Tendo em vista que o Brasil é um grande produtor e consumidor de café, fica evidente a importância de sua caracterização em diferentes locais de cultivo. O objetivo do presente estudo foi quantificar os teores de metais pesados em amostras de grãos de café, provenientes de lavouras implantadas em solos oriundos do basalto e do arenito Caiuá do Estado do Paraná. Foram examinadas 30 amostras de grãos de café, sendo 15 cultivados em solos predominantemente arenosos e 15 em solos argilosos. A determinação de Cromo (Cr), Cobalto (Co), Níquel (Ni), Chumbo (Pb), Cádmio (Cd), Cobre (Cu), Zinco (Zn) e Manganês (Mn) foi feita por espectrometria de emissão atômica com fonte de plasma induzido com Thermo Jarrel-ash ICAP 612 E (ICP-EAS). Os níveis foram variáveis entre as amostras.

Interação da torra e moagem do café na preferência do consumidor do oeste paranaense / Influence of roasting and milling on consumers coffee preference at Paraná west-Brazil

O objetivo deste estudo foi avaliar as preferências dos consumidores de café da região oeste do Paraná, em relação ao ponto de torra e à granulometria de moagem. Foram realizadas análises sensoriais de preferência e físico-químicas. As análises físico-químicas foram utilizadas para monitorar as mudanças ocorridas nos produtos ao longo do processo de torra. Os cafés com torras mais leves foram pouco aceitos. O café que apresentou aparência global, aroma e sabor preferido foi o que apresentava uma torra média escura, mais próxima das torras tradicionais brasileiras. A torra mais escura foi bem aceita apenas em relação à aparência. A moagem fina foi preferida em relação à aparência global do produto, mas, apesar de ter apresentado maior acidez, os provadores não diferenciaram seu sabor.

This study aimed the evaluation of the consumers preference for coffee in the west region of Paraná State, Brazil, in relation to the roasting degree and grinding granulometry. Sensorial preference and physical-chemichal analysis were made. The physical-chemichal analysis were used to monitor the changes that occurred in the products along the roasting process. The samples of coffee with light toasts were little accepted. The kind of coffee that presented preferred global appearance, aroma and taste was the one which presented average dark toasting, which is the one that is more similar to the traditional Brazilians toastings. The darkest toasting, was well-accepted in relation to appearance. The thin milling was preferred in relation to the global appearance of the product, but in spite of having presented major acidity the tasters did not make difference about its taste.

Interação da torra e moagem do café na preferência do consumidor do oeste paranaense / Influence of roasting and milling on consumers coffee preference at Paraná west-Brazil

O objetivo deste estudo foi avaliar as preferências dos consumidores de café da região oeste do Paraná, em relação ao ponto de torra e à granulometria de moagem. Foram realizadas análises sensoriais de preferência e físico-químicas. As análises físico-químicas foram utilizadas para monitorar as mudanças ocorridas nos produtos ao longo do processo de torra. Os cafés com torras mais leves foram pouco aceitos. O café que apresentou aparência global, aroma e sabor preferido foi o que apresentava uma torra média escura, mais próxima das torras tradicionais brasileiras. A torra mais escura foi bem aceita apenas em relação à aparência. A moagem fina foi preferida em relação à aparência global do produto, mas, apesar de ter apresentado maior acidez, os provadores não diferenciaram seu sabor.(AU)

This study aimed the evaluation of the consumers preference for coffee in the west region of Paraná State, Brazil, in relation to the roasting degree and grinding granulometry. Sensorial preference and physical-chemichal analysis were made. The physical-chemichal analysis were used to monitor the changes that occurred in the products along the roasting process. The samples of coffee with light toasts were little accepted. The kind of coffee that presented preferred global appearance, aroma and taste was the one which presented average dark toasting, which is the one that is more similar to the traditional Brazilians toastings. The darkest toasting, was well-accepted in relation to appearance. The thin milling was preferred in relation to the global appearance of the product, but in spite of having presented major acidity the tasters did not make difference about its taste.(AU)