RESUMO
HLA-DR beta 1*04 subtypes associated with rheumatoid arthritis (RA) were studied by polymerase chain reaction with sequence specific primers and the 86th amino acid of the DR beta 1 chain was analysed in RA patients vs healthy controls. The frequency of HLA-DR beta 1*0401 and 0404/0407 alleles was significantly increased in RA patients vs the controls, HLA-DR beta 1*0401 and 0404 being the dominant variants in RA. In most RA patients hydrophilic glycine proved to be the 86th amino acid of the peptide binding region thus it could have an effect on the development of RA.
Assuntos
Artrite Reumatoide/genética , Artrite Reumatoide/imunologia , Antígeno HLA-DR1/genética , Alelos , Sequência de Aminoácidos , Sequência de Bases , Primers do DNA , Glicina/genética , Antígeno HLA-DR1/imunologia , Antígeno HLA-DR4/genética , Antígeno HLA-DR4/imunologia , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Homologia de Sequência de AminoácidosRESUMO
Two years results of methotrexate treatment (13 patients, 7.5-10 mg weekly dose) in elderly onset rheumatoid arthritis are reported in comparison to other therapies (10 patients, im. gold 50 mg weekly or per os steroid max. 10 mg daily). Clinical activity of rheumatoid arthritis was measured with standard rheumatological parameters whereas progression of the disease was defined by means of a radiological method. Methotrexate caused better clinical improvement and retarded progression to a greater extent than the other therapies. Methotrexate achieved its maximum effect during the first 6 months of therapy. Methotrexate was well tolerable. Long term applicability of low dose methotrexate treatment in elderly age is emphasized.
Assuntos
Artrite Reumatoide/tratamento farmacológico , Metotrexato/uso terapêutico , Fatores Etários , Idoso , Relação Dose-Resposta a Droga , Feminino , Ouro/uso terapêutico , Humanos , Masculino , Metotrexato/administração & dosagem , Prednisolona/uso terapêuticoRESUMO
Poisoning by paraquat, a plant-protecting agent, its clinical manifestations and treatment are discussed. The case of a 5-year-old boy who had ingested an unknown quantity of paraquat is described. Peritoneal dialysis proved to be effective in overcoming renal and hepatic failure. Subsequently, a pulmonary lesion with unilateral preponderance developed; this showed marked radiological regression and in a year nearly complete functional recovery ensued.