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1.
Science ; 373(6559): 1156-1161, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34516839

RESUMO

Dominant mutations in ubiquitously expressed transfer RNA (tRNA) synthetase genes cause axonal peripheral neuropathy, accounting for at least six forms of Charcot-Marie-Tooth (CMT) disease. Genetic evidence in mouse and Drosophila models suggests a gain-of-function mechanism. In this study, we used in vivo, cell type­specific transcriptional and translational profiling to show that mutant tRNA synthetases activate the integrated stress response (ISR) through the sensor kinase GCN2 (general control nonderepressible 2). The chronic activation of the ISR contributed to the pathophysiology, and genetic deletion or pharmacological inhibition of Gcn2 alleviated the peripheral neuropathy. The activation of GCN2 suggests that the aberrant activity of the mutant tRNA synthetases is still related to translation and that inhibiting GCN2 or the ISR may represent a therapeutic strategy in CMT.


Assuntos
Doença de Charcot-Marie-Tooth/metabolismo , Glicina-tRNA Ligase/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Estresse Fisiológico , Tirosina-tRNA Ligase/metabolismo , Fator 4 Ativador da Transcrição/genética , Fator 4 Ativador da Transcrição/metabolismo , Animais , Doença de Charcot-Marie-Tooth/genética , Doença de Charcot-Marie-Tooth/fisiopatologia , Modelos Animais de Doenças , Feminino , Deleção de Genes , Genes Dominantes , Glicina-tRNA Ligase/genética , Masculino , Camundongos , Camundongos Mutantes , Neurônios Motores/fisiologia , Biossíntese de Proteínas , Inibidores de Proteínas Quinases/farmacologia , Inibidores de Proteínas Quinases/uso terapêutico , Proteínas Serina-Treonina Quinases/antagonistas & inibidores , Proteínas Serina-Treonina Quinases/genética , Medula Espinal/fisiopatologia , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/genética , Estresse Fisiológico/fisiologia , Transcriptoma , Tirosina-tRNA Ligase/genética
2.
Science ; 350(6267): 1477-8, 2015 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-26680183
3.
J R Soc Interface ; 12(104): 20141055, 2015 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-25589573

RESUMO

Cadherin interactions ensure the correct registry and anchorage of cells during tissue formation. Along the plasma membrane, cadherins form inter-junctional lattices via cis- and trans-dimerization. While structural studies have provided models for cadherin interactions, the molecular nature of cadherin binding in vivo remains unexplored. We undertook a multi-disciplinary approach combining live cell imaging of three-dimensional cell assemblies (spheroids) with a computational model to study the dynamics of N-cadherin interactions. Using a loss-of-function strategy, we demonstrate that each N-cadherin interface plays a distinct role in spheroid formation. We found that cis-dimerization is not a prerequisite for trans-interactions, but rather modulates trans-interfaces to ensure tissue stability. Using a model of N-cadherin junction dynamics, we show that the absence of cis-interactions results in low junction stability and loss of tissue integrity. By quantifying the binding and unbinding dynamics of the N-cadherin binding interfaces, we determined that mutating either interface results in a 10-fold increase in the dissociation constant. These findings provide new quantitative information on the steps driving cadherin intercellular adhesion and demonstrate the role of cis-interactions in junction stability.


Assuntos
Junções Aderentes/fisiologia , Caderinas/química , Esferoides Celulares/fisiologia , Cálcio/química , Adesão Celular , Membrana Celular/metabolismo , Movimento Celular , Simulação por Computador , Crioultramicrotomia , Dimerização , Humanos , Imageamento Tridimensional , Mutação , Probabilidade , Ligação Proteica , Software , Propriedades de Superfície
4.
Curr Opin Chem Biol ; 17(5): 738-46, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23938204

RESUMO

The non-canonical amino acid labeling techniques BONCAT (bioorthogonal non-canonical amino acid tagging) and FUNCAT (fluorescent non-canonical amino acid tagging) enable the specific identification and visualization of newly synthesized proteins. Recently, these techniques have been applied to neuronal systems to elucidate protein synthesis dynamics during plasticity, identify stimulation-induced proteomes and subproteomes and to investigate local protein synthesis in specific subcellular compartments. The next generation of tools and applications, reviewed here, includes the development of new tags, the quantitative identification of newly synthesized proteins, the application of NCAT to whole animals, and the ability to genetically restrict NCAT labeling. These techniques will enable not only improved detection but also allow new scientific questions to be tackled.


Assuntos
Aminoácidos/metabolismo , Plasticidade Neuronal , Aminoácidos/química , Animais , Corantes Fluorescentes/análise , Corantes Fluorescentes/química , Biossíntese de Proteínas , Proteínas/análise , Proteínas/química , Proteínas/metabolismo
5.
Neuroscience ; 131(2): 547-55, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15708495

RESUMO

The spontaneous or background discharge patterns of in vivo single neuron is mostly considered as neuronal noise, which is assumed to be devoid of any correlation between successive inter-spike-intervals (ISI). Such random fluctuations are modeled only statistically by stochastic point process, lacking any temporal correlation. In this study, we have investigated the nature of spontaneous irregular fluctuations of single neurons from human hippocampus-amygdala complex by three different methods: (i) detrended fluctuation analysis (DFA), (ii) multiscale entropy (MSE), (iii) rate estimate convergence. Both the DFA and MSE analysis showed the presence of long-range power-law correlation over time in the ISI sequences. Moreover, we observed that the individual spike trains presented non-random structure on longer time-scales and showed slow convergence of rate estimates with increasing counting time. This power-law correlation and the slow convergence of statistical moments were eliminated by randomly shuffling the ISIs even though the distributions of ISIs were preserved. Thus the power-law relationship arose from long-term correlations among ISIs that were destroyed by shuffling the data. Further, we found that neurons which showed long-range correlations also showed statistically significant correlated firing as measured by correlation coefficient or mutual information function. The presence of long-range correlations indicates the history-effect or memory in the firing pattern by the associative formation of a neuronal assembly.


Assuntos
Potenciais de Ação/fisiologia , Tonsila do Cerebelo/fisiologia , Hipocampo/fisiologia , Neurônios/fisiologia , Adulto , Feminino , Humanos , Análise dos Mínimos Quadrados , Masculino , Pessoa de Meia-Idade , Distribuição Normal , Fatores de Tempo
7.
J Neurophysiol ; 86(2): 1037-42, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11495971

RESUMO

Virus-mediated gene transfer into neurons is a powerful tool for the analysis of neuronal structure and function. Recombinant sindbis virus has been previously used to study protein function in hippocampal neuron cultures as well as in hippocampal organotypic slice cultures. Nevertheless, some concern still exists about the physiological relevance of these cultured preparations. Acute hippocampal slices are a widely used preparation for the study of synaptic transmission, but currently recombinant gene delivery is usually achieved only through time-consuming transgenic techniques. In this study, we show that a subregion of the CA1 area in acute hippocampal slices can be specifically altered to express a gene of interest. A sindbis virus vector carrying an enhanced green fluorescent protein (EGFP) reporter was injected in vivo into the hippocampus of adult rats. After 18 h, rats were killed, and acute hippocampal slices, infected in the CA1 field, were analyzed morphologically and electrophysiologically. Infected slices showed healthy and stable electrophysiological responses as well as long-term potentiation. In addition, infected pyramidal cells were readily recognized in living slices by two-photon imaging. Specifically, the introduction of an EGFP-Actin fusion protein greatly enhanced the detection of fine processes and dendritic spines. We propose this technique as an efficient tool for studying gene function in adult hippocampal neurons.


Assuntos
Técnicas de Transferência de Genes , Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Sindbis virus/genética , Animais , Dendritos/fisiologia , Eletrofisiologia , Expressão Gênica/fisiologia , Genes Reporter , Proteínas de Fluorescência Verde , Indicadores e Reagentes/metabolismo , Proteínas Luminescentes/genética , Masculino , Microscopia Confocal , Plasticidade Neuronal/fisiologia , Técnicas de Cultura de Órgãos , Ratos , Ratos Sprague-Dawley
8.
Neuron ; 30(2): 489-502, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11395009

RESUMO

Using pharmacological approaches, several recent studies suggest that local protein synthesis is required for synaptic plasticity. Convincing demonstrations of bona fide dendritic protein synthesis in mammalian neurons are rare, however. We developed a protein synthesis reporter in which the coding sequence of green fluorescent protein is flanked by the 5' and 3' untranslated regions from CAMKII-alpha, conferring both dendritic mRNA localization and translational regulation. In cultured hippocampal neurons, we show that BDNF, a growth factor involved in synaptic plasticity, stimulates protein synthesis of the reporter in intact, mechanically, or "optically" isolated dendrites. The stimulation of protein synthesis is blocked by anisomycin and not observed in untreated neurons. In addition, dendrites appear to possess translational hot spots, regions near synapses where protein synthesis consistently occurs over time.


Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/genética , Hipocampo/fisiologia , Neurônios/fisiologia , Biossíntese de Proteínas/fisiologia , Regiões 3' não Traduzidas/genética , Regiões 5' não Traduzidas/genética , Animais , Animais Recém-Nascidos , Anisomicina/farmacologia , Fator Neurotrófico Derivado do Encéfalo/farmacologia , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina , Células Cultivadas , Dendritos/fisiologia , Genes Reporter , Proteínas de Fluorescência Verde , Hipocampo/citologia , Proteínas Luminescentes/análise , Proteínas Luminescentes/genética , Neurônios/citologia , Biossíntese de Proteínas/efeitos dos fármacos , Inibidores da Síntese de Proteínas/farmacologia , RNA Mensageiro/análise , Ratos , Transcrição Gênica/efeitos dos fármacos , Transcrição Gênica/fisiologia , Transfecção
9.
Annu Rev Neurosci ; 24: 299-325, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11283313

RESUMO

Studies over the past 20 years have revealed that gene expression in neurons is carried out by a distributed network of translational machinery. One component of this network is localized in dendrites, where polyribosomes and associated membranous elements are positioned beneath synapses and translate a particular population of dendritic mRNAs. The localization of translation machinery and mRNAs at synapses endows individual synapses with the capability to independently control synaptic strength through the local synthesis of proteins. The present review discusses recent studies linking synaptic plasticity to dendritic protein synthesis and mRNA trafficking and considers how these processes are regulated. We summarize recent information about how synaptic signaling is coupled to local translation and to the delivery of newly transcribed mRNAs to activated synaptic sites and how local translation may play a role in activity-dependent synaptic modification.


Assuntos
Dendritos/fisiologia , Hipocampo/fisiologia , Proteínas do Tecido Nervoso/genética , Sinapses/fisiologia , Animais , Humanos , Potenciação de Longa Duração , Proteínas do Tecido Nervoso/biossíntese , Plasticidade Neuronal , Biossíntese de Proteínas , RNA Mensageiro/genética , Transcrição Gênica
10.
Neuroscience ; 99(1): 107-17, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10924956

RESUMO

Aspirin (acetylsalicylic acid), and its main metabolite sodium salicylate, have been shown to protect neurons from excitotoxic cell death in vitro. The objective of our study was to investigate the possible neuroprotective effects of sodium salicylate in vivo in rats with kainic acid-induced seizures, a model for temporal lobe epilepsy in human patients. Male Sprague-Dawley rats received intraperitoneal injections of kainic acid either alone, or with sodium salicylate given before and for 40h after kainic acid injections. The control group received either phosphate-buffered saline or sodium salicylate without co-administration of kainic acid. Animals developed status epilepticus, which was aborted 1.5-2h later with diazepam. On day 3 following kainic acid-induced seizures, animals received bromodeoxyuridine to measure cellular proliferation, and were killed under anesthesia 24h later. Brains were removed, sectioned, and analysed for gross histological changes, evidence of hemorrhage, DNA fragmentation, cellular proliferation, and microglial immunohistochemistry. We report that sodium salicylate did not protect neurons from seizure-induced cell death, and to the contrary, it caused focal hemorrhage and cell death in the hippocampal formation and the entorhinal/piriform cortex of rats with kainic acid-induced seizures. Hemorrhage was never observed in animals that received vehicle, kainic acid or sodium salicylate only, which indicated that sodium salicylate exerted its effect only in animals with seizures, and was confined to select regions of the brain that undergo seizure activity. Large numbers of cells displaying DNA fragmentation were detected in the hippocampal formation, entorhinal/piriform cortex and the dorsomedial thalamic nucleus of rats that received kainic acid or kainic acid in combination with sodium salicylate. Bromodeoxyuridine immunohistochemistry revealed large numbers of proliferating cells in and around the areas with most severe neural injury induced by kainic acid or kainic acid co-administered with sodium salicylate. These same brain regions displayed intense staining with a microglia-specific marker, an indication of microglial activation in response to brain damage. In all cases, the degree of cell death, cell proliferation and microglia staining was more severe in animals that received the combination of kainic acid and sodium salicylate when compared to animals that received kainic acid alone. We hypothesize that our findings are attributable to sodium salicylate-induced blockade of cellular mechanisms that protect cells from calcium-mediated injury. These initial observations may have important clinical implications for patients with epilepsy who take aspirin while affected by these conditions, and should promote further investigation of this relationship.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Hemorragia Cerebral/induzido quimicamente , Hipocampo/efeitos dos fármacos , Microglia/efeitos dos fármacos , Convulsões , Salicilato de Sódio/farmacologia , Animais , Aspirina/metabolismo , Morte Celular/efeitos dos fármacos , Morte Celular/fisiologia , Contraindicações , Agonistas de Aminoácidos Excitatórios , Hipocampo/citologia , Hipocampo/lesões , Ácido Caínico , Masculino , Microglia/fisiologia , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Fármacos Neuroprotetores , Ratos , Ratos Sprague-Dawley , Convulsões/induzido quimicamente
11.
Neurosci Lett ; 282(3): 141-4, 2000 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-10717411

RESUMO

Recent studies have demonstrated the importance of neurotrophin function in adult synaptic plasticity. In an effort to characterize the intracellular signaling pathways that couple Trk receptor activation to the final physiological effects of neurotrophins, we have examined the role of intracellular calcium rises in neurotrophin-induced synaptic enhancement in hippocampal slices. Using pharmacological blockers to two different calcium ion (Ca(2+)) sources, voltage-gated Ca(2+) channels and intracellular Ca(2+) stores, we show that the potentiating effects of neurotrophins in hippocampal slices are mediated by intracellular Ca(2+) signaling. Although basal synaptic transmission between hippocampal CA3 and CA1 neurons was not affected by nifedipine or thapsigargin, both drugs significantly attenuated brain-derived neurotrophic factor or neurotrophin-3-induced synaptic enhancement. The pharmacological blockade of Ca(2+) signaling is effective only during the initial period of neurotrophin-induced potentiation. These data suggest that the minimal requirements for inducing potentiation by neurotrophins involve a transient increase in intracellular Ca(2+) concentration, via voltage-gated Ca(2+) channels and/or intracellular Ca(2+) stores.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/fisiologia , Sinalização do Cálcio/fisiologia , Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Neurotrofina 3/fisiologia , Animais , Fator Neurotrófico Derivado do Encéfalo/farmacologia , Cálcio/metabolismo , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/fisiologia , Eletrofisiologia , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Técnicas In Vitro , Ativação do Canal Iônico , Masculino , Neurotrofina 3/farmacologia , Ratos , Ratos Sprague-Dawley , Transmissão Sináptica/fisiologia
12.
J Neurophysiol ; 82(6): 3213-22, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10601455

RESUMO

CA1 pyramidal cells are the primary output neurons of the hippocampus, carrying information about the result of hippocampal network processing to the subiculum and entorhinal cortex (EC) and thence out to the rest of the brain. The primary excitatory drive to the CA1 pyramidal cells comes via the Schaffer collateral (SC) projection from area CA3. There is also a direct projection from EC to stratum lacunosum-moleculare (SLM) of CA1, an input well positioned to modulate information flow through the hippocampus. High-frequency stimulation in SLM evokes an inhibition sufficiently strong to prevent CA1 pyramidal cells from spiking in response to SC input, a phenomenon we refer to as spike-blocking. We characterized the spike-blocking efficacy of burst stimulation (10 stimuli at 100 Hz) in SLM and found that it is greatest at approximately 300-600 ms after the burst, consistent with the time course of the slow GABA(B) signaling pathway. Spike-blocking efficacy increases in potency with the number of SLM stimuli in a burst, but also decreases with repeated presentations of SLM bursts. Spike-blocking was eliminated in the presence of GABA(B) antagonists. We have identified a candidate population of interneurons in SLM and distal stratum radiatum (SR) that may mediate this spike-blocking effect. We conclude that the output of CA1 pyramidal cells, and hence the hippocampus, is modulated in an input pattern-dependent manner by activation of the direct pathway from EC.


Assuntos
Hipocampo/fisiologia , Células Piramidais/fisiologia , Animais , Axônios/fisiologia , Giro Denteado/citologia , Giro Denteado/fisiologia , Estimulação Elétrica , Eletrodos , Eletrofisiologia , Hipocampo/citologia , Técnicas In Vitro , Interneurônios/fisiologia , Masculino , Potenciais da Membrana/fisiologia , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley
13.
Curr Opin Cell Biol ; 11(5): 549-53, 1999 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-10508654

RESUMO

Studies in the past few years suggest that cell adhesion molecules may play signaling as well as structural roles at adult synapses during plasticity. The observation that many adhesion molecules are expressed both pre-synaptically and post-synaptically raises the possibility that information about synaptic activity might simultaneously be communicated to both sides of the synapse, circumventing the need for distinct anterograde and retrograde messengers.


Assuntos
Comportamento Animal/fisiologia , Moléculas de Adesão Celular/fisiologia , Proteínas do Tecido Nervoso/fisiologia , Plasticidade Neuronal/fisiologia , Animais , Caderinas/genética , Caderinas/fisiologia , Moléculas de Adesão Celular Neuronais/fisiologia , Drosophila melanogaster/metabolismo , Hipocampo , Humanos , Proteínas de Insetos/fisiologia , Integrinas/fisiologia , Aprendizagem em Labirinto/fisiologia , Camundongos , Camundongos Knockout , Camundongos Mutantes Neurológicos , Família Multigênica , Oligopeptídeos/química , Sinapses/fisiologia
14.
Learn Mem ; 6(3): 267-75, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10492008

RESUMO

Neurotrophic factors, including BDNF and NT-3, have been implicated in the regulation of synaptic transmission and plasticity. Previous attempts to analyze synaptic transmission and plasticity in mice lacking the NT-3 gene have been hampered by the early death of the NT-3 homozygous knockout animals. We have bypassed this problem by examining synaptic transmission in mice in which the NT-3 gene is deleted in neurons later in development, by crossing animals expressing the CRE recombinase driven by the synapsin I promoter to animals in which the NT-3 gene is floxed. We conducted blind field potential recordings at the Schaffer collateral-CA1 synapse in hippocampal slices from homozygous knockout and wild-type mice. We examined the following indices of synaptic transmission: (1) input-output relationship; (2) paired-pulse facilitation; (3) post-tetanic potentiation; and (4) long-term potentiation: induced by two different protocols: (a) two trains of 100-Hz stimulation and (b) theta burst stimulation. We found no difference between the knockout and wild-type mice in any of the above measurements. These results suggest that neuronal NT-3 does not play an essential role in normal synaptic transmission and some forms of plasticity in the mouse hippocampus.


Assuntos
Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Fatores de Crescimento Neural/genética , Fatores de Crescimento Neural/fisiologia , Neurônios/fisiologia , Transmissão Sináptica/fisiologia , Animais , Northern Blotting , Southern Blotting , Eletrofisiologia , Deleção de Genes , Hipocampo/citologia , Processamento de Imagem Assistida por Computador , Técnicas In Vitro , Camundongos , Camundongos Knockout , Neurotrofina 3 , Ratos
15.
J Neurosci ; 19(18): 7823-33, 1999 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-10479685

RESUMO

mRNA for the alpha-subunit of CaMKII is abundant in dendrites of neurons in the forebrain (Steward, 1997). Here we show that tetanic stimulation of the Schaffer collateral pathway causes an increase in the concentration of alpha-CaMKII in the dendrites of postsynaptic neurons. The increase is blocked by anisomycin and is detected by both quantitative immunoblot and semiquantitative immunocytochemistry. The increase in dendritic alpha-CaMKII can be measured 100-200 micrometer away from the neuronal cell bodies as early as 5 min after a tetanus. Transport mechanisms for macromolecules from neuronal cell bodies are not fast enough to account for this rapid increase in distal portions of the dendrites. Therefore, we conclude that dendritic protein synthesis must produce a portion of the newly accumulated CaMKII. The increase in concentration of dendritic CaMKII after tetanus, together with the previously demonstrated increase in autophosphorylated CaMKII (Ouyang et al., 1997), will produce a prolonged increase in steady-state kinase activity in the dendrites, potentially influencing mechanisms of synaptic plasticity that are controlled through phosphorylation by CaMKII.


Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/genética , Corpo Estriado/fisiologia , Dendritos/enzimologia , Regulação Enzimológica da Expressão Gênica , Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Neurônios/fisiologia , Vias Aferentes/fisiologia , Animais , Anisomicina/farmacologia , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina , Dendritos/efeitos dos fármacos , Estimulação Elétrica , Técnicas In Vitro , Potenciação de Longa Duração/efeitos dos fármacos , Masculino , Neurônios/efeitos dos fármacos , Neurônios/enzimologia , Fosforilação , Ratos , Ratos Sprague-Dawley
17.
Curr Opin Neurobiol ; 9(1): 105-9, 1999 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10072368

RESUMO

Examples of signaling molecules that are devoted to neuronal development at the exclusion of other functions are scarce. It may then come as no surprise to learn that a family of molecules that promote neuronal survival, differentiation and outgrowth also regulate synaptic transmission at both developing and mature synapses. Indeed, many studies over the past five years have shown that neurotrophins, including nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5 and brain-derived neurotrophic factor (BDNF), have both rapid and long-latency influences on synaptic strength. New research has highlighted the enormous range of neurotrophin actions at both developing and mature synapses, demonstrating that transmission can be enhanced or reduced at excitatory and inhibitory synapses by either pre- or postsynaptic mechanisms.


Assuntos
Fatores de Crescimento Neural/fisiologia , Transmissão Sináptica/fisiologia , Animais , Fator Neurotrófico Derivado do Encéfalo/fisiologia , Camundongos , Camundongos Knockout , Plasticidade Neuronal
18.
J Neurophysiol ; 81(3): 1036-44, 1999 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10085331

RESUMO

The temporoammonic pathway, the direct projection from layer III of the entorhinal cortex to area CA1 of the hippocampus, includes both excitatory and inhibitory components that are positioned to be an important source of modulation of the hippocampal output. However, little is known about synaptic plasticity in this pathway. We used field recordings in hippocampal slices prepared from mature (6- to 8-wk old) rats to study long-term depression (LTD) in the temporoammonic pathway. Low-frequency (1 Hz) stimulation (LFS) for 10 min resulted in a depression of the field response that lasted for >/=1 h. This depression was saturable by multiple applications of LFS. LTD induction was unaffected by the blockade of either fast (GABAA) or slow (GABAB) inhibition. Temporoammonic LTD was inhibited by the presence of the N-methyl-D-aspartate (NMDA) receptor antagonist AP5, suggesting a dependence on calcium influx. Full recovery from depression could be induced by high-frequency (100 Hz) stimulation (HFS); in the presence of the GABAA antagonist bicuculline, HFS induced recovery above the original baseline level. Similarly, HFS or theta-burst stimulation (TBS) applied to naive slices caused little potentiation, whereas HFS or TBS applied in the presence of bicuculline resulted in significant potentiation of the temporoammonic response. Our results show that, unlike the Schaffer collateral input to CA1, the temporoammonic input in mature animals is easy to depress but difficult to potentiate.


Assuntos
Hipocampo/fisiologia , Plasticidade Neuronal , Transmissão Sináptica/fisiologia , Lobo Temporal/fisiologia , Animais , Estimulação Elétrica , Antagonistas de Aminoácidos Excitatórios/farmacologia , Técnicas In Vitro , Masculino , Antagonistas Muscarínicos/farmacologia , Vias Neurais/fisiologia , Ratos , Ratos Sprague-Dawley , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Transmissão Sináptica/efeitos dos fármacos , Ácido gama-Aminobutírico/fisiologia
19.
J Neurosci ; 18(24): 10231-40, 1998 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-9852560

RESUMO

Brain-derived neurotrophic factor (BDNF), like other neurotrophins, has long-term effects on neuronal survival and differentiation; furthermore, recent work has shown that BDNF also can induce rapid changes in synaptic efficacy. We have investigated the mechanism(s) of these synaptic effects on cultured embryonic hippocampal neurons. In the presence of the GABAA receptor antagonist, picrotoxin, the application of BDNF (100 ng/ml) for 1-5 min increased the amplitude of evoked synaptic currents by 48 +/- 9% in 10 of 15 pairs of neurons and increased the frequency of EPSC bursts to 205 +/- 20% of the control levels. There was no detectable effect of BDNF on various measures of electrical excitability, including the resting membrane potential, input resistance, action potential threshold, and action potential amplitude. In addition, BDNF did not change the postsynaptic currents induced by the exogenous application of glutamate. BDNF did increase the frequency of miniature EPSCs (mEPSCs) (268.0 +/- 46.8% of control frequency), however, without affecting the mEPSC amplitude. The effect of BDNF on mEPSC frequency was blocked by the tyrosine kinase inhibitor K252a and also by the removal of extracellular calcium ([Ca2+]o). Fura-2 recordings showed that BDNF elicited an increase in intracellular calcium concentration ([Ca2+]c). This effect was dependent on [Ca2+]o; it was blocked by K252a and by thapsigargin, but not by caffeine. The results demonstrate that BDNF enhances glutamatergic synaptic transmission at a presynaptic locus and that this effect is accompanied by a rise in [Ca2+]c that requires the release of Ca2+ from IP3-gated stores.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/farmacologia , Hipocampo/fisiologia , Neurônios/fisiologia , Neurotransmissores/metabolismo , Animais , Cálcio/metabolismo , Células Cultivadas , Relação Dose-Resposta a Droga , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Espaço Extracelular/metabolismo , Antagonistas de Receptores de GABA-A , Ácido Glutâmico/farmacologia , Hipocampo/metabolismo , Técnicas In Vitro , Líquido Intracelular/metabolismo , Potenciais da Membrana/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Técnicas de Patch-Clamp , Ratos , Ratos Wistar , Transmissão Sináptica/efeitos dos fármacos , Fatores de Tempo
20.
Proc Natl Acad Sci U S A ; 95(18): 10884-9, 1998 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-9724799

RESUMO

We have developed a method to analyze the relative contributions of pre- and postsynaptic actions of a particular gene product in neurons in culture and potentially in slices using adenovirus-mediated gene transfer. A recombinant virus directed the expression of both a GFP reporter protein and TrkB.T1, a C-terminal truncated dominant negative TrkB neurotrophin receptor. When expressed in the presynaptic cell at synapses between embryonic hippocampal neurons in culture, the dominant negative TrkB.T1 inhibited two forms of synaptic potentiation induced by the neurotrophin brain-derived neurotrophic factor (BDNF): (i) greater evoked synaptic transmission and (ii) higher frequency of spontaneous miniature synaptic currents. These inhibition effects are not seen if the transgene is expressed only in the postsynaptic cell. We conclude that BDNF-TrkB signal transduction in the presynaptic terminal leads to both types of potentiation and is therefore the primary cause of synaptic enhancement by BDNF in these neurons.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/fisiologia , Hipocampo/metabolismo , Potenciação de Longa Duração/fisiologia , Neurônios/metabolismo , Receptores Proteína Tirosina Quinases/metabolismo , Receptores de Fator de Crescimento Neural/metabolismo , Animais , Células Cultivadas , Feminino , Hipocampo/citologia , Hipocampo/fisiologia , Potenciais da Membrana , Neurônios/fisiologia , Gravidez , Ratos , Ratos Wistar , Receptor do Fator Neutrófico Ciliar , Transdução de Sinais , Transmissão Sináptica/fisiologia
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