RESUMO
There is interest in developing potent, selective, and cell-permeable inhibitors of human ferrous iron and 2-oxoglutarate (2OG) oxygenases for use in functional and target validation studies. The 3-component Betti reaction enables efficient one-step C-7 functionalisation of modified 8-hydroxyquinolines (8HQs) to produce cell-active inhibitors of KDM4 histone demethylases and other 2OG oxygenases; the work exemplifies how a template-based metallo-enzyme inhibitor approach can be used to give biologically active compounds.
Assuntos
Inibidores Enzimáticos/farmacologia , Oxigenases/antagonistas & inibidores , Oxiquinolina/farmacologia , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Humanos , Modelos Moleculares , Estrutura Molecular , Oxigenases/metabolismo , Oxiquinolina/síntese química , Oxiquinolina/química , Relação Estrutura-AtividadeRESUMO
The analysis of the genetic basis of phenotypic traits is moving towards the complex diseases prevalent in wealthy populations. There is an increasing requirement for the detection of different types of sequence variation, particularly single-nucleotide polymorphisms (SNPs). SNPs occur about once every 100 to 300 bases. High-density SNP maps will help to identify the multiple genes associated with complex diseases such as cancer, diabetes, vascular disease, and some forms of mental illness.
Assuntos
DNA/análise , Polimorfismo de Nucleotídeo Único , DNA/genética , Predisposição Genética para Doença/genética , HumanosAssuntos
Imunização , Saúde da População Rural , Adolescente , Adulto , Fatores Etários , Vacina BCG/administração & dosagem , Pré-Escolar , Vacina contra Difteria, Tétano e Coqueluche/administração & dosagem , Feminino , Humanos , Esquemas de Imunização , Lactente , Masculino , Vacina contra Sarampo/administração & dosagem , Somália , VacinaçãoRESUMO
The ECL gene detection system is a novel, sensitive, non-radioactive system for the detection of nucleic acid hybridized on both nylon and nitrocellulose membranes. It is characterized by direct labeling of probe sequences with horseradish peroxidase combined with an enhanced chemiluminescent (ECL) detection reaction; the light output is captured on blue-light sensitive film. The application of the system to a range of standard molecular biology hybridization techniques is described.
