1.
J Microbiol Methods
; 103: 118-23, 2014 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24929037
RESUMO
A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.