Diagnostic value of anti-VZV IgG in neurological diseases among varicella unvaccinated individuals.

Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that causes neurological manifestations either as a complication of primary infection or reactivation. VZV induced neurological diseases have a good prognosis when confirmed early and treated with anti-viral therapy. Myelitis, encephalitis, ventriculitis or meningitis can occur without a telltale rash in immunocompetent and immunocompromised individuals making the diagnosis difficult. We analyzed CSF and serum samples from 30 unvaccinated study participants (17 male and 13 female) to determine the presence of VZV DNA by PCR in CSF and to estimate serum and CSF anti-VZV IgG and albumin levels in participants with neurological manifestations with/without rash. Anti-VZV IgG was detected in CSF (n = 22, [73%]) and serum (n = 29, [97%]) of pediatric and adult participants. Anti-VZV IgG were detected in CSF of participants with varied clinical presentation altered sensorium (n = 8, [36%]), meningitis (n = 4, [18%]), acute febrile illness (n = 3, [14%], encephalopathy/meningoencephalitis (n = 2, [9%]), irritability (n = 2, [9%]) and each patient from cerebrovascular stroke, demyelinating disorder and febrile seizure (n = 1, [4.5%]). VZV DNA was detected from one participant and CSF serum albumin levels were elevated in 53% of study participants. VZV DNA is present up to 1-2 weeks post onset of disease, after which anti-VZV antibody may be the only indicator of disease and therefore both VZV DNA and anti-VZV IgG need to be tested for in CSF. As VZV DNA and VZV IgG antibody are both good indicators of VZV reactivation, routine testing would result in reduced morbidity and mortality by early detection of disease and antiviral treatment.

Expanded spectrum of varicella disease and the need for vaccination in India.

Varicella vaccine was first licensed in Japan and South Korea in 1989 for use in healthy children and was introduced in US in 1995. So far, 29 countries have adopted varicella vaccine in their universal immunization program (UIP). No Asian country, India included, has adopted the varicella vaccine as part of their UIP. The extra-cutaneous sites for VZV diseases are central nervous system and gastrointestinal tract, the expanded disease spectrum includes vasculopathy, myelitis, inflammatory bowel disease, perforated ulcers, and gastritis. The actual disease burden of varicella is not known as most of the infected individuals may not visit the physician. The amplifiable VZV DNA will not always be detectable in cerebrospinal fluid (CSF) samples in protracted illnesses such as vasculopathies, but demonstrable anti-VZV IgG in CSF has diagnostic value. The World Health Organization (WHO) position paper 2014 recommends two doses of varicella and zoster vaccines in targeted population. In India, varicella vaccine is not included in the UIP due to the cost and the belief that lifelong immunity occurs following primary infection. The expanded spectrum of VZV disease and the mounting body of evidence, however, suggest the need for both varicella and zoster vaccines in routine immunization schedule.

Experimental and numerical investigation of the effect of temporal variation in ionic strength on colloid retention and remobilization in saturated porous media.

Temporal variations in the chemistry of infiltrating water into the subsurface are known to cause remobilization of colloids from the grain surfaces, thereby increasing the travel distance of the colloidal contaminants. Hence, it is essential to thoroughly understand the transport, deposition, and release mechanisms of colloids in the subsurface, through laboratory experiments and modeling. There are only a few experiments in which the chemistry of inflow water is changed rapidly during colloid transport. Also, although some models have been presented for simulating the effect of transient chemistry on the fate of colloids, there is no consensus in this regard, as the proposed models suffer from shortcomings. In this study, we systematically investigated the effect of temporal variations in ionic strength on the remobilization of deposited colloids in saturated porous media through laboratory column experiments and numerical modeling. Four sets of column experiments were performed, in which we injected carboxylate-modified latex colloids at a given ionic strength for a specified period. After breakthrough of colloids, the ionic strength of inflowing water was decreased in a stepwise manner to 0 mM (DI water). The initial ionic strength values of the four experiments were 100, 50, 25, and 10 mM. We observed partial release of deposited colloids after several steps of ionic strength decrease with significant release observed only when the ionic strength was reduced to below 10 mM. We also found that the fraction of released colloids decreased with increasing value of initial ionic strength of inflow water. We have developed a mathematical model incorporating a novel formulation for ionic strength-dependent deposition and release. The model is found to capture the colloid breakthrough curves reasonably well for all experiments with the same set of parameter values, except the one at the initial ionic strength of 25 mM.

A two-way coupled model for the co-transport of two different colloids in porous media.

Models for the co-transport of two different colloids commonly assume a one-way coupling. This is because often a large colloid and small colloid are involved. Therefore, they assume that the spread of smaller colloid is affected by the transport of larger colloids, but not the other way around. However, a number of studies have shown that this assumption is not valid, even for large and small colloids. Therefore, in this study, a two-way coupled model is developed to simulate the co-transport of two different colloids in porous media and their effect on each other. We have considered the interactions of the two colloids with the grain surface, kinetics of heteroaggregation (of the two colloids), and heteroaggregate deposition onto the grain surface. We assumed a first-order kinetic model to represent heteroaggregate formation and its deposition on the grain surface. The model is evaluated by fitting the experimental data reported in four different papers from the literature on the co-transport of clay colloids and viruses, bacteria and graphene oxide nanoparticles, and clay colloids and graphene oxide nanoparticles. The model performance is compared with the commonly-used one-way coupled model. The two-way coupled model is found to satisfactorily simulate most of the experimental conditions reported in the above papers, except for the co-transport of montmorillonite-adenovirus, and Staphylococcus aureus- graphene oxide nanoparticles.

Upscaling of nanoparticle transport in porous media under unfavorable conditions: Pore scale to Darcy scale.

Transport and deposition of nanoparticles in porous media is a multi-scale problem governed by several pore-scale processes, and hence, it is critical to link the processes at pore scale to the Darcy-scale behavior. In this study, using pore network modeling, we develop correlation equations for deposition rate coefficients for nanoparticle transport under unfavorable conditions at the Darcy scale based on pore-scale mechanisms. The upscaling tool is a multi-directional pore-network model consisting of an interconnected network of pores with variable connectivities. Correlation equations describing the pore-averaged deposition rate coefficients under unfavorable conditions in a cylindrical pore, developed in our earlier studies, are employed for each pore element. Pore-network simulations are performed for a wide range of parameter values to obtain the breakthrough curves of nanoparticle concentration. The latter is fitted with macroscopic 1-D advection-dispersion equation with a two-site linear reversible deposition accounting for both equilibrium and kinetic sorption. This leads to the estimation of three Darcy-scale deposition coefficients: distribution coefficient, kinetic rate constant, and the fraction of equilibrium sites. The correlation equations for the Darcy-scale deposition coefficients, under unfavorable conditions, are provided as a function of measurable Darcy-scale parameters, including: porosity, mean pore throat radius, mean pore water velocity, nanoparticle radius, ionic strength, dielectric constant, viscosity, temperature, and surface potentials of the particle and grain surfaces. The correlation equations are found to be consistent with the available experimental results, and in qualitative agreement with Colloid Filtration Theory for all parameters, except for the mean pore water velocity and nanoparticle radius.

Modeling the co-transport of viruses and colloids in unsaturated porous media.

A mathematical model is developed to simulate the co-transport of viruses and colloids in unsaturated porous media under steady-state flow conditions. The virus attachment to the mobile and immobile colloids is described using a linear reversible kinetic model. Colloid transport is assumed to be decoupled from virus transport; that is, we assume that colloids are not affected by the presence of attached viruses on their surface. The governing equations are solved numerically using an alternating three-step operator splitting approach. The model is verified by fitting three sets of experimental data published in the literature: (1) Syngouna and Chrysikopoulos (2013) and (2) Walshe et al. (2010), both on the co-transport of viruses and clay colloids under saturated conditions, and (3) Syngouna and Chrysikopoulos (2015) for the co-transport of viruses and clay colloids under unsaturated conditions. We found a good agreement between observed and fitted breakthrough curves (BTCs) under both saturated and unsaturated conditions. Then, the developed model was used to simulate the co-transport of viruses and colloids in porous media under unsaturated conditions, with the aim of understanding the relative importance of various processes on the co-transport of viruses and colloids in unsaturated porous media. The virus retention in porous media in the presence of colloids is greater during unsaturated conditions as compared to the saturated conditions due to: (1) virus attachment to the air-water interface (AWI), and (2) co-deposition of colloids with attached viruses on its surface to the AWI. A sensitivity analysis of the model to various parameters showed that the virus attachment to AWI is the most sensitive parameter affecting the BTCs of both free viruses and total mobile viruses and has a significant effect on all parts of the BTC. The free and the total mobile viruses BTCs are mainly influenced by parameters describing virus attachment to the AWI, virus interaction with mobile and immobile colloids, virus attachment to solid-water interface (SWI), and colloid interaction with SWI and AWI. The virus BTC is relatively insensitive to parameters describing the maximum adsorption capacity of the AWI for colloids, inlet colloid concentration, virus detachment rate coefficient from the SWI, maximum adsorption capacity of the AWI for viruses and inlet virus concentration.

Virus-sized colloid transport in a single pore: model development and sensitivity analysis.

A mathematical model is developed to simulate the transport and deposition of virus-sized colloids in a cylindrical pore throat considering various processes such as advection, diffusion, colloid-collector surface interactions and hydrodynamic wall effects. The pore space is divided into three different regions, namely, bulk, diffusion and potential regions, based on the dominant processes acting in each of these regions. In the bulk region, colloid transport is governed by advection and diffusion whereas in the diffusion region, colloid mobility due to diffusion is retarded by hydrodynamic wall effects. Colloid-collector interaction forces dominate the transport in the potential region where colloid deposition occurs. The governing equations are non-dimensionalized and solved numerically. A sensitivity analysis indicates that the virus-sized colloid transport and deposition is significantly affected by various pore-scale parameters such as the surface potentials on colloid and collector, ionic strength of the solution, flow velocity, pore size and colloid size. The adsorbed concentration and hence, the favorability of the surface for adsorption increases with: (i) decreasing magnitude and ratio of surface potentials on colloid and collector, (ii) increasing ionic strength and (iii) increasing pore radius. The adsorbed concentration increases with increasing Pe, reaching a maximum value at Pe=0.1 and then decreases thereafter. Also, the colloid size significantly affects particle deposition with the adsorbed concentration increasing with increasing particle radius, reaching a maximum value at a particle radius of 100nm and then decreasing with increasing radius. System hydrodynamics is found to have a greater effect on larger particles than on smaller ones. The secondary minimum contribution to particle deposition has been found to increase as the favorability of the surface for adsorption decreases. The sensitivity of the model to a given parameter will be high if the conditions are favorable for adsorption. The results agree qualitatively with the column-scale experimental observations available in the literature. The current model forms the building block in upscaling colloid transport from pore scale to Darcy scale using Pore-Network Modeling.

Gaseous and bioaerosol emissions from municipal wastewater treatment plants.

Wastewater treatment plants (WWTPs) are identified as potential emission sources of greenhouse gases (GHGs) and bioaerosols. This paper reviews and analyse the potential sources of GHGs and bioaerosols from different unit operations and processes of WWTPs. Aeration tanks of activated sludge process (ASP) are found to be the most important sources of GHGs as well as bioaerosol emissions. Nitrification and denitrification processes are found to be important sources of nitrous oxide (N2O) emissions. To minimize the N2O emissions from WWTPs, dissolved oxygen (DO) concentration should be kept greater than 2 mg/L in nitrification process, whereas purely anoxic condition (0 mg/L DO) is required in denitrification process. Diffused aeration emits fewer microbes into the air than surface aerators. It is observed that fixed-film processes emit microbes by two orders of magnitude less than aeration tanks. The various WWTPs discussed in this study used different methods of treatment sample collection and species of microorganisms studied. It is realised that the standardisation of the microorganisms to be analysed and methods of sample collection needs to be done. It is also found that from the microbiological point of view, there is no clean air in the vicinity of a WWTP.

Effect of organic shock loads on a two-stage activated sludge-biofilm reactor.

The effects of short-term organic shock loads on the performance of a laboratory scale two-stage activated sludge (AS)-biofilm reactor working at 6 h HRT and treating medium strength domestic wastewater was studied by increasing the influent chemical oxygen demand (COD) to 2-4 times the normal values. Each shock load was applied for a period of 6 h, after which normal loading conditions were resumed. The maximum effluent COD concentration obtained was 169, 169, 250 and 617 mg/L under the shock loads of 808, 1170, 1358 and 1900 mg COD/L, respectively. The COD removal rate increased with increasing effective OLR. The system recovered quickly from shock loads; recovery time proportional to the magnitude of shock loads. The optimum loading rate of the reactor was found at effective OLR of 170 mg COD/m(3)/h. Shock loads changed the dominant bacterial type in the reactor from Gram-positive rods to Gram-negative oval shaped bacteria.