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Diabetes Metab ; 44(1): 55-60, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28916390

RESUMO

AIM: Kidney hypoxia can predispose to the development of acute and chronic renal failure in diabetes. Ischaemia-reperfusion injury (IRI) causes inflammation, and diabetes is known to exacerbate this inflammatory response in the kidney, whereas alarmin IL-33 could act as an innate immune mediator during kidney IRI. Thus, the present study examined the impact of genetic IL-33 receptor ST2 deficiency (ST2-/-) on renal IRI in euglycaemic and hyperglycaemic mice. METHODS: Hyperglycaemia was induced with streptozotocin (STZ) in adult male C57BL/6JRj wild-type (WT) mice and ST2-/- mice. Unilateral renal IRI was achieved 3months after STZ treatment by left kidney nephrectomy (non-ischaemic control kidney) and clamping of the right renal artery for 32min in STZ- and vehicle-treated animals. At 24h after reperfusion, renal function and injury were determined by levels of plasma creatinine, blood urea nitrogen (BUN) and histological tubule scores. Also, in a complementary pilot clinical study, soluble ST2 concentrations were compared in diabetics and non-diabetics. RESULTS: Urinary albumin was significantly increased in STZ-induced hyperglycaemic mice, regardless of genotypic background. At 24h post-ischaemia, plasma creatinine, BUN and tubular injury were significantly reduced in ST2-/- mice compared with vehicle-treated WT mice, but this protective effect was lost in the STZ-induced hyperglycaemic ST2-/- animals. Plasma concentrations of soluble ST2 were significantly greater in type 2 diabetes patients vs non-diabetics. CONCLUSION: Our data suggest that the IL-33/ST2 pathway exerts differential effects depending on the glucose environment, opening-up new avenues for future research on alarmins and diabetes in ischaemia-related diseases.


Assuntos
Injúria Renal Aguda/metabolismo , Glicemia/metabolismo , Hiperglicemia/metabolismo , Proteína 1 Semelhante a Receptor de Interleucina-1/genética , Traumatismo por Reperfusão/metabolismo , Idoso , Animais , Diabetes Mellitus Experimental , Feminino , Humanos , Hiperglicemia/induzido quimicamente , Proteína 1 Semelhante a Receptor de Interleucina-1/metabolismo , Rim/metabolismo , Masculino , Camundongos , Camundongos Knockout , Pessoa de Meia-Idade , Estreptozocina/efeitos adversos
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