Monitoring Changes in TMS-Evoked EEG and EMG Activity During 1†Hz rTMS of the Healthy Motor Cortex.

Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation technique capable of inducing neuroplasticity as measured by changes in peripheral muscle electromyography (EMG) or electroencephalography (EEG) from pre-to-post stimulation. However, temporal courses of neuromodulation during ongoing rTMS are unclear. Monitoring cortical dynamics via TMS-evoked responses using EMG (motor-evoked potentials; MEPs) and EEG (transcranial-evoked potentials; TEPs) during rTMS might provide further essential insights into its mode of action - temporal course of potential modulations. The objective of this study was to first evaluate the validity of online rTMS-EEG and rTMS-EMG analyses, and second to scrutinize the temporal changes of TEPs and MEPs during rTMS. As rTMS is subject to high inter-individual effect variability, we aimed for single-subject analyses of EEG changes during rTMS. Ten healthy human participants were stimulated with 1,000 pulses of 1 Hz rTMS over the motor cortex, while EEG and EMG were recorded continuously. Validity of MEPs and TEPs measured during rTMS was assessed in sensor and source space. Electrophysiological changes during rTMS were evaluated with model fitting approaches on a group- and single-subject level. TEPs and MEPs appearance during rTMS was consistent with past findings of single pulse experiments. Heterogeneous temporal progressions, fluctuations or saturation effects of brain activity were observed during rTMS depending on the TEP component. Overall, global brain activity increased over the course of stimulation. Single-subject analysis revealed inter-individual temporal courses of global brain activity. The present findings are in favor of dose-response considerations and attempts in personalization of rTMS protocols.

Retest reliability of repetitive transcranial magnetic stimulation over the healthy human motor cortex: a systematic review and meta-analysis.

Introduction: Repetitive transcranial magnetic stimulation (rTMS) is used to induce long-lasting changes (aftereffects) in cortical excitability, which are often measured via single-pulse TMS (spTMS) over the motor cortex eliciting motor-evoked potentials (MEPs). rTMS includes various protocols, such as theta-burst stimulation (TBS), paired associative stimulation (PAS), and continuous rTMS with a fixed frequency. Nevertheless, subsequent aftereffects of rTMS are variable and seem to fail repeatability. We aimed to summarize standard rTMS procedures regarding their test-retest reliability. Hereby, we considered influencing factors such as the methodological quality of experiments and publication bias. Methods: We conducted a literature search via PubMed in March 2023. The inclusion criteria were the application of rTMS, TBS, or PAS at least twice over the motor cortex of healthy subjects with measurements of MEPs via spTMS as a dependent variable. The exclusion criteria were measurements derived from the non-stimulated hemisphere, of non-hand muscles, and by electroencephalography only. We extracted test-retest reliability measures and aftereffects from the eligible studies. With the Rosenthal fail-safe N, funnel plot, and asymmetry test, we examined the publication bias and accounted for influential factors such as the methodological quality of experiments measured with a standardized checklist. Results: A total of 15 studies that investigated test-retest reliability of rTMS protocols in a total of 291 subjects were identified. Reliability measures, i.e., Pearson's r and intraclass correlation coefficient (ICC) applicable from nine studies, were mainly in the small to moderate range with two experiments indicating good reliability of 20 Hz rTMS (r = 0.543) and iTBS (r = 0.55). The aftereffects of rTMS procedures seem to follow the heuristics of respective inhibition or facilitation, depending on the protocols' frequency, and application pattern. There was no indication of publication bias and the influence of methodological quality or other factors on the reliability of rTMS. Conclusion: The reliability of rTMS appears to be in the small to moderate range overall. Due to a limited number of studies reporting test-retest reliability values and heterogeneity of dependent measures, we could not provide generalizable results. We could not identify any protocol as superior to the others.

On the reliability of motor evoked potentials in hand muscles of healthy adults: a systematic review.

Aims: Motor evoked potentials (MEP) elicited by transcranial magnetic stimulation (TMS) over the primary motor cortex are used as a neurophysiological marker of cortical excitability in clinical and scientific practice. Though, the reliability of this outcome parameter has not been clarified. Using a systematic approach, this work reviews and critically appraises studies on the reliability of MEP outcome parameters derived from hand muscles of healthy subjects and gives a proposal for most reliable TMS practice. Methods: A systematic literature research was performed in PubMed, according to the PRISMA guidelines. Articles published up to March 2023 that were written in English, conducted repeated measurements from hand muscles of healthy subjects and reliability analysis were included. The risk of publication bias was determined. Two authors conducted the literature search and rated the articles in terms of eligibility and methodological criteria with standardized instruments. Frequencies of the checklist criteria were calculated and inter-rater reliability of the rating procedure was determined. Reliability and stimulation parameters were extracted and summarized in a structured way to conclude best-practice recommendation for reliable measurements. Results: A total of 28 articles were included in the systematic review. Critical appraisal of the studies revealed methodological heterogeneity and partly contradictory results regarding the reliability of outcome parameters. Inter-rater reliability of the rating procedure was almost perfect nor was there indication of publication bias. Identified studies were grouped based on the parameter investigated: number of applied stimuli, stimulation intensity, reliability of input-output curve parameters, target muscle or hemisphere, inter-trial interval, coil type or navigation and waveform. Conclusion: The methodology of studies on TMS is still subject to heterogeneity, which could contribute to the partly contradictory results. According to the current knowledge, reliability of the outcome parameters can be increased by adjusting the experimental setup. Reliability of single pulse MEP measurement could be optimized by using (1) at least five stimuli per session, (2) a minimum of 110% resting motor threshold as stimulation intensity, (3) a minimum of 4 s inter-trial interval and increasing the interval up to 20 s, (4) a figure-of-eight coil and (5) a monophasic waveform. MEPs can be reliably operationalized.

Tendon compliance and preload must be considered when determining the in vivo force-velocity relationship from the torque-angular velocity relation.

In vivo, the force-velocity relation (F-v-r) is typically derived from the torque-angular velocity relation (T-ω-r), which is subject to two factors that may influence resulting measurements: tendon compliance and preload prior to contraction. The in vivo plantar flexors' T-ω-r was determined during preloaded maximum voluntary shortening contractions at 0-200°/s. Additionally, we used a two factor block simulation study design to independently analyze the effects of preload and tendon compliance on the resulting T-ω-r. Therefore, we replicated the in vivo experiment using a Hill-type muscle model of the gastrocnemius medialis. The simulation results matched a key pattern observed in our recorded in vivo experimental data: during preloaded contractions, torque output of the muscle was increased when compared with non-preloaded contractions from literature. This effect increased with increasing contraction velocity and can be explained by a rapidly recoiling tendon, allowing the contractile element to contract more slowly, thus developing higher forces compared with non-preloaded contractions. Our simulation results also indicate that a more compliant tendon results in increased ankle joint torques. The simulation and the experimental data clearly show that the deduction of the in vivo F-v-r from the T-ω-r is compromised due to the two factors preloading and tendon compliance.

The Relationship Between Lower Limb Passive Muscle and Tendon Compression Stiffness and Oxygen Cost During Running.

Studies have reported that a stiff triceps surae muscle and tendon-aponeurosis and also a more compliant quadriceps muscle and tendon-aponeurosis, are related to lower oxygen cost during running. However, to date, no study has investigated in a single experiment how oxygen cost during running is related to the stiffness of the free tendons (Achilles tendon, patellar tendon) and all the superficial muscles of two major muscle groups for running (i.e., quadriceps, triceps surae). Thus, 17 male trained runners/triathletes participated in this study and visited the laboratory on three occasions. On the first day, the participants were familiarized with the tests. On the second day, the passive compression stiffness of the triceps surae muscle (i.e., gastrocnemii), Achilles tendon, quadriceps muscle (i.e., vastii, rectus femoris), and patellar tendon was non-invasively measured using a digital palpation device (MyotonPRO). In addition, an incremental test was applied to test the VO2max of the participants. Thereafter, in the third visit, after at least 48-h of rest, participants performed a 15-min run on the treadmill with a speed reflecting a velocity of 70% VO2max, to assess oxygen costs during running. The Spearman correlation showed a significant negative correlation between passive Achilles tendon compression stiffness and running oxygen consumption, with a large effect size (rρ = -0.52; CI (95%) -0.81 to -0.33; P = 0.03). Moreover, no further significant relationship between oxygen cost during running and the passive compression stiffness of the quadriceps muscle and patellar tendon, as well as the triceps surae muscle, was detected. The significant correlation indicates that a stiffer passive Achilles tendon can lead to a lower oxygen cost during running. Future studies will have to test the causality of this relationship with training methods such as strength training that are able to increase the Achilles tendon stiffness.

Semi-automated motor hotspot search (SAMHS): a framework toward an optimised approach for motor hotspot identification.

Background: Motor hotspot identification represents the first step in the determination of the motor threshold and is the basis for the specification of stimulation intensity used for various Transcranial Magnetic Stimulation (TMS) applications. The level of experimenters' experience and the methodology of motor hotspot identification differ between laboratories. The need for an optimized and time-efficient technique for motor hotspot identification is therefore substantial. Objective: With the current work, we present a framework for an optimized and time-efficient semi-automated motor hotspot search (SAMHS) technique utilizing a neuronavigated robot-assisted TMS system (TMS-cobot). Furthermore, we aim to test its practicality and accuracy by a comparison with a manual motor hotspot identification method. Method: A total of 32 participants took part in this dual-center study. At both study centers, participants underwent manual hotspot search (MHS) with an experienced TMS researcher, and the novel SAMHS procedure with a TMS-cobot (hereafter, called cobot hotspot search, CHS) in a randomized order. Resting motor threshold (RMT), and stimulus intensity to produce 1 mV (SI1mV) peak-to-peak of motor-evoked potential (MEP), as well as MEPs with 120% RMT and SI1mV were recorded as outcome measures for comparison. Results: Compared to the MHS method, the CHS produced lower RMT, lower SI1mV and a trend-wise higher peak-to-peak MEP amplitude in stimulations with SI1mV. The duration of the CHS procedure was longer than that of the MHS (15.60 vs. 2.43 min on average). However, accuracy of the hotspot was higher for the CHS compared to the MHS. Conclusions: The SAMHS procedure introduces an optimized motor hotspot determination system that is easy to use, and strikes a fairly good balance between accuracy and speed. This new procedure can thus be deplored by experienced as well as beginner-level TMS researchers.

What to stretch? - Isolated proprioceptive neuromuscular facilitation stretching of either quadriceps or triceps surae followed by post-stretching activities alters tissue stiffness and jump performance.

To overcome a possible drop in performance following longer stretch durations (>60 s), post-stretching dynamic activities (PSA) can be applied. However, it is not clear if this is true for isolated proprioceptive neuromuscular facilitation (PNF) stretching of different muscle groups (e.g., triceps surae and quadriceps). Thus, 16 participants performed both interventions (triceps surae PNF + PSA; quadriceps PNF + PSA) in random order, separated by 48 h. Jump performance was assessed with a force plate, and tissue stiffness was assessed with a MyotonPro device. While no changes were detected in the countermovement jump performance, the PNF + PSA interventions resulted in a decrease in drop jump performance which led to a large magnitude of change following the triceps surae PNF + PSA and a small-to-medium magnitude of change following the quadriceps PNF + PSA. Moreover, in the triceps surae PNF + PSA intervention, a decrease in Achilles tendon stiffness was seen, while in the quadriceps PNF + PSA intervention, a decrease in the overall quadriceps muscle stiffness was seen. According to our results, we recommend that especially triceps surae stretching is avoided during warm-up (also when PSA is included) when the goal is to optimise explosive or reactive muscle contractions.

Validation of a new inertial measurement unit system based on different dynamic movements for future in-field applications.

Using inertial measurement units (IMUs) in monitoring and analysing sport movements has become popular in sports research since it avoids the laboratory limitation. However, the accuracy of modern IMU-systems (hardware combined with software) needs to be validated using gold-standard systems as baseline. In this study, we investigated the feasibility of the aktos-t IMU-system for in-field biomechanical research by comparing its outputs in various tasks (repetitive movements, gait and jumping) undertaken by 14 participants, with those of an optoelectronic system. The results showed that the accuracy of aktos-t varies according to the task performed. The accuracy of pelvis, hip and knee joints ranged between acceptable (root mean squared error (RMSE) < 5°) and tolerable (RMSE < 10°) in gait, while the upper limb joints showed inaccuracy (RMSE > 10°) and imprecision (coefficient of repeatability > 10°) during the repetitive movement test. Jump impact appeared not to influence the IMU outcomes (p > 0.05). The main sources of error could be related to the IMU-alignment during the reference T-pose. Finally, the study provides researchers the means for evaluating the accuracy of aktos-t (hardware, software and biomechanical model) as sufficiently precise for its application in their in-field investigations.

Residual force enhancement in humans: Is there a true non-responder?

When an active muscle is stretched and kept isometrically active, the resulting force is enhanced compared to a purely isometric reference contraction at the same muscle length and activity; a generally accepted muscle property called residual force enhancement (rFE). Interestingly, studies on voluntary muscle action regularly identify a significant number of participants not showing rFE. Therefore, the aim was to unmask possible confounders for this non-responsive behavior. Ten participants performed maximum voluntary isometric plantarflexion contractions with and without preceding stretch. Contractions were accompanied by the assessment of voluntary activation using the twitch-interpolation technique. The same test protocol was repeated four additional times with a least on day rest in-between. Additionally, at the first and fifth sessions, a submaximal tetanic muscle-stimulation condition was added. At both muscle-stimulation sessions mean rFE higher 10% (p < 0.028) was found. In contrast, during voluntary muscle action, individual participants showed inconsistent rFE across sessions and only one session (#3) had significant rFE (5%; p = 0.023) in group means. As all participants clearly had rFE in electrical stimulation conditions, structural deficits cannot explain the missing rFE in voluntary muscle action. However, we also did not find variability in voluntary activation levels or muscle activity as the confounding characteristics of "non-responders."

Power Amplification Increases With Contraction Velocity During Stretch-Shortening Cycles of Skinned Muscle Fibers.

Muscle force, work, and power output during concentric contractions (active muscle shortening) are increased immediately following an eccentric contraction (active muscle lengthening). This increase in performance is known as the stretch-shortening cycle (SSC)-effect. Recent findings demonstrate that the SSC-effect is present in the sarcomere itself. More recently, it has been suggested that cross-bridge (XB) kinetics and non-cross-bridge (non-XB) structures (e.g., titin and nebulin) contribute to the SSC-effect. As XBs and non-XB structures are characterized by a velocity dependence, we investigated the impact of stretch-shortening velocity on the SSC-effect. Accordingly, we performed in vitro isovelocity ramp experiments with varying ramp velocities (30, 60, and 85% of maximum contraction velocity for both stretch and shortening) and constant stretch-shortening magnitudes (17% of the optimum sarcomere length) using single skinned fibers of rat soleus muscles. The different contributions of XB and non-XB structures to force production were identified using the XB-inhibitor Blebbistatin. We show that (i) the SSC-effect is velocity-dependent-since the power output increases with increasing SSC-velocity. (ii) The energy recovery (ratio of elastic energy storage and release in the SSC) is higher in the Blebbistatin condition compared with the control condition. The stored and released energy in the Blebbistatin condition can be explained by the viscoelastic properties of the non-XB structure titin. Consequently, our experimental findings suggest that the energy stored in titin during the eccentric phase contributes to the SSC-effect in a velocity-dependent manner.

Functional Adaptations in Isokinetic Performance and Shoulder Mobility in Elite Ultimate Frisbee Players.

Ultimate Frisbee is a team sport where players mainly use their dominant arm for throwing a flying disc. Little is known about possible functional adaptations to this unilateral strain. The purpose of this study was to investigate shoulder rotation strength and range of motion (ROM) in elite Ultimate Frisbee players. We were especially interested in possible sport-specific functional adaptations of peak torque and work ratios as well as the range of motion of agonist and antagonist shoulder rotators regarding laterality between the dominant and non-dominant body sides. For this reason, the concentric external and internal isokinetic (60°/s) shoulder rotation torque and passive glenohumeral rotation ROM were examined in 15 male subjects (27.5 ±â€Š5.5 yrs; all current or former members of the German National Team) and comparisons of dominant to non-dominant body sides were conducted. Maximum strength (work) during external shoulder rotation was higher on the dominant body side compared with the non-dominant body side (8.4 ±â€Š8.4 %; p < 0.01). No side-to-side differences were found in maximum internal rotation strength. Internal rotation ROM and total ROM were smaller on the dominant body side (21 ± 18 %; p < 0.01 and 5 ±â€Š9 %; p < 0.05). Our findings indicate similar adaptation patterns of shoulder rotation mobility compared to other overhead sports, but differing results regarding shoulder rotation strength. Stretching exercises and monitoring programs are recommended for elite Ultimate Frisbee players, starting at the early stages of the career.

Contribution of Stretch-Induced Force Enhancement to Increased Performance in Maximal Voluntary and Submaximal Artificially Activated Stretch-Shortening Muscle Action.

In everyday muscle action or exercises, a stretch-shortening cycle (SSC) is performed under different levels of intensity. Thereby, compared to a pure shortening contraction, the shortening phase in a SSC shows increased force, work, and power. One mechanism to explain this performance enhancement in the SSC shortening phase is, besides others, referred to the phenomenon of stretch-induced increase in muscle force (known as residual force enhancement; rFE). It is unclear to what extent the intensity of muscle action influences the contribution of rFE to the SSC performance enhancement. Therefore, we examined the knee torque, knee kinematics, m. vastus lateralis fascicle length, and pennation angle changes of 30 healthy adults during isometric, shortening (CON) and stretch-shortening (SSC) conditions of the quadriceps femoris. We conducted maximal voluntary contractions (MVC) and submaximal electrically stimulated contractions at 20%, 35%, and 50% of MVC. Isometric trials were performed at 20° knee flexion (straight leg: 0°), and dynamic trials followed dynamometer-driven ramp profiles of 80°-20° (CON) and 20°-80°-20° (SSC), at an angular velocity set to 60°/s. Joint mechanical work during shortening was significantly (p < 0.05) enhanced by up to 21% for all SSC conditions compared to pure CON contractions at the same intensity. Regarding the steady-state torque after the dynamic phase, we found significant torque depression for all submaximal SSCs compared to the isometric reference contractions. There was no difference in the steady-state torque after the shortening phases between CON and SSC conditions at all submaximal intensities, indicating no stretch-induced rFE that persisted throughout the shortening. In contrast, during MVC efforts, the steady-state torque after SSC was significantly less depressed compared to the steady-state torque after the CON condition (p = 0.034), without significant differences in the m. vastus lateralis fascicle length and pennation angle. From these results, we concluded that the contribution of the potential enhancing factors in SSCs of the m. quadriceps femoris is dependent on the contraction intensity and the type of activation.

Considerations on the human Achilles tendon moment arm for in vivo triceps surae muscle-tendon unit force estimates.

Moment arm-angle functions (MA-a-functions) are commonly used to estimate in vivo muscle forces in humans. However, different MA-a-functions might not only influence the magnitude of the estimated muscle forces but also change the shape of the muscle's estimated force-angle relationship (F-a-r). Therefore, we investigated the influence of different literature based Achilles tendon MA-a-functions on the triceps surae muscle-tendon unit F-a-r. The individual in vivo triceps torque-angle relationship was determined in 14 participants performing maximum voluntary fixed-end plantarflexion contractions from 18.3° ± 3.2° plantarflexion to 24.2° ± 5.1° dorsiflexion on a dynamometer. The resulting F-a-r were calculated using 15 literature-based in vivo Achilles tendon MA-a-functions. MA-a-functions affected the F-a-r shape and magnitude of estimated peak active triceps muscle-tendon unit force. Depending on the MA-a-function used, the triceps was solely operating on the ascending limb (n = 2), on the ascending limb and plateau region (n = 12), or on the ascending limb, plateau region and descending limb of the F-a-r (n = 1). According to our findings, the estimated triceps muscle-tendon unit forces and the shape of the F-a-r are highly dependent on the MA-a-function used. As these functions are affected by many variables, we recommend using individual Achilles tendon MA-a-functions, ideally accounting for contraction intensity-related changes in moment arm magnitude.

Cross-Bridges and Sarcomeric Non-cross-bridge Structures Contribute to Increased Work in Stretch-Shortening Cycles.

Stretch-shortening cycles (SSCs) refer to the muscle action when an active muscle stretch is immediately followed by active muscle shortening. This combination of eccentric and concentric contractions is the most important type of daily muscle action and plays a significant role in natural locomotion such as walking, running or jumping. SSCs are used in human and animal movements especially when a high movement speed or economy is required. A key feature of SSCs is the increase in muscular force and work during the concentric phase of a SSC by more than 50% compared with concentric muscle actions without prior stretch (SSC-effect). This improved muscle capability is related to various mechanisms, including pre-activation, stretch-reflex responses and elastic recoil from serial elastic tissues. Moreover, it is assumed that a significant contribution to enhanced muscle capability lies in the sarcomeres itself. Thus, we investigated the force output and work produced by single skinned fibers of rat soleus muscles during and after ramp contractions at a constant velocity. Shortening, lengthening, and SSCs were performed under physiological boundary conditions with 85% of the maximum shortening velocity and stretch-shortening magnitudes of 18% of the optimum muscle length. The different contributions of cross-bridge (XB) and non-cross-bridge (non-XB) structures to the total muscle force were identified by using Blebbistatin. The experiments revealed three main results: (i) partial detachment of XBs during the eccentric phase of a SSC, (ii) significantly enhanced forces and mechanical work during the concentric phase of SSCs compared with shortening contractions with and without XB-inhibition, and (iii) no residual force depression after SSCs. The results obtained by administering Blebbistatin propose a titin-actin interaction that depends on XB-binding or active XB-based force production. The findings of this study further suggest that enhanced forces generated during the active lengthening phase of SSCs persist during the subsequent shortening phase, thereby contributing to enhanced work. Accordingly, our data support the hypothesis that sarcomeric mechanisms related to residual force enhancement also contribute to the SSC-effect. The preload of the titin molecule, acting as molecular spring, might be part of that mechanism by increasing the mechanical efficiency of work during physiological SSCs.

High-density electromyographic assessment of stretch reflex activity during drop jumps from varying drop heights.

This study investigated how drop heights and their associated drop jump performance relate to stretch reflex modulations. Eleven male subjects performed ten drop jumps from each of three individually predetermined drop heights. These were the drop height resulting in maximal performance (OPT), as well as 10 cm below (LOW) and above (HIGH) maximal performance. To quantify drop jump performance the reactive strength index, derived from force plate measures, was used. High-density surface EMG provided both stretch reflex response timing and size, as well as novel insight into the associated motor unit recruitment via muscle fiber conduction velocity estimations. These measures were examined in the vastus lateralis (VL), soleus (SOL) and gastrocnemius medialis (GM). Drop jump performance improved by 9% (p < 0.001) from LOW to OPT and decreased by 5% (p = 0.008) from OPT to HIGH. Despite decreasing performance, stretch reflex responses were largest at HIGH. Stretch reflex responses timing did not change; staying within the short (SOL, <60 ms) and medium (VL, GM; 60-85 ms) latency response time-frames. Motor unit recruitment appeared to change across drop heights only for VL, whereas activation intensity only changed for SOL. These results indicate that during drop jumps above OPT neuromuscular modifications result in VL no longer being maximally recruited.

Force depression following a stretch-shortening cycle depends on the amount of residual force enhancement established in the initial stretch phase.

Studies on residual force enhancement (rFE) and residual force depression (rFD) of the muscle-tendon unit (MTU) have typically been conducted independent of each other, with little information available on how stretch-induced rFE affects the shortening phase and the steady-state MTU isometric force at the end of stretch-shortening cycles (SSCs). We showed previously that when rFE is kept constant, but the force at the end of the stretch is varied by changing the stretch speed, the steady-state forces at the end of SSCs were the same. These results led to the hypothesis that the amount of rFE of the MTU established in the initial stretch phase of SSCs determines the steady-state force following the shortening phase of SSCs. This study was aimed at testing this hypothesis. Steady-state MTU isometric thumb adduction forces were measured for pure isometric contractions, following pure shortening contractions, following pure stretch contractions, and following SSCs with constant shortening speed and magnitude. However, two stretch magnitudes (30° and 10° thumb abduction) and stretch speeds (15°/sec and ~ 60°/sec, respectively) were chosen such that forces at the end of the stretch phase of the SSCs were the same, while rFE differed substantially. As hypothesized, the steady-state isometric MTU forces following SSCs were positively related to the stretch-magnitude dependent amount of rFE established in the stretch phase and were independent of the force reached at the end of the stretch phase in SSCs. Among many competing theories, these results can potentially be explained with the idea that there is a length-specific engagement of a passive structural element at the initial length of muscle activation.

Accuracy and precision of loadsol® insole force-sensors for the quantification of ground reaction force-based biomechanical running parameters.

Force plates represent the "gold standard" in measuring running kinetics to predict performance or to identify the sources of running-related injuries. As these measurements are generally limited to laboratory analyses, wireless high-quality sensors for measuring in the field are needed. This work analysed the accuracy and precision of a new wireless insole forcesensor for quantifying running-related kinetic parameters. Vertical ground reaction force (GRF) was simultaneously measured with pit-mounted force plates (1 kHz) and loadsol® sensors (100 Hz) under unshod forefoot and rearfoot running-step conditions. GRF data collections were repeated four times, each separated by 30 min treadmill running, to test influence of extended use. A repeated-measures ANOVA was used to identify differences between measurement devices. Additionally, mean bias and Bland-Altman limits of agreement (LoA) were calculated. We found a significant difference (p < .05) in ground contact time, peak force, and force rate, while there was no difference in parameters impulse, time to peak, and negative force rate. There was no influence of time point of measurement. The mean bias of ground contact time, impulse, peak force, and time to peak ranged between 0.6% and 3.4%, demonstrating high accuracy of loadsol® devices for these parameters. For these same parameters, the LoA analysis showed that 95% of all measurement differences between insole and force plate measurements were less than 12%, demonstrating high precision of the sensors. However, highly dynamic behaviour of GRF, such as force rate, is not yet sufficiently resolved by the insole devices, which is likely explained by the low sampling rate.

Force depression following a stretch-shortening cycle is independent of stretch peak force and work performed during shortening.

The steady-state isometric force following active muscle shortening or lengthening is smaller (force depression; FD) or greater (residual force enhancement; RFE) than a purely isometric contraction at the corresponding length. The mechanisms behind these phenomena remain not fully understood, with few studies investigating the effects of FD and RFE in stretch-shortening cycles (SSC). The purpose of this study was to investigate the influence of RFE and peak force at the end of the stretch phase on the steady-state isometric force following shortening. Isometric thumb adduction force measurements were preceded by an isometric, a shortening contraction to induce FD, and SSCs at different stretch speeds (15°/s, 60°/s, and 120°/s). The different peak force values at the end of stretch and the different amounts of work performed during shortening did not influence the steady-state isometric force at the end of the SSC. We conclude that the FD following SSC depends exclusively on the amount of RFE established in the initial stretch phase in situations where the timing and contractile conditions of the shortening phase are kept constant .

Physiological Responses to Firefighting in Extreme Temperatures Do Not Compare to Firefighting in Temperate Conditions.

Purpose: The aim of this study was to examine physiological responses to two different simulated firefighting exercises: a firefighting exercise with flashovers, smoke, poor visibility and extreme temperatures (300°) in a burning container and a standard firefighting exercise in temperate conditions. Furthermore, a second purpose of the study was to find out if the contribution of strength and endurance capacities to firefighting performance changes when the demands of the firefighting exercise change. Methods: Sixteen professional firefighters performed a maximum treadmill test, strength testing, a standard simulated firefighting exercise (SFE) without heat and flashovers and a firefighting exercise with a simulation of the flashover phenomenon in a burning container (FOT). The treadmill testing was used to determine peak oxygen uptake (VO2peak), ventilatory threshold (VT1) and respiratory compensation point (RCP). Three intensity zones were identified according to heart rate (HR) values corresponding to VT1 and RCP: zone 1-HR below VT1, zone 2-HR between VT1 and RCP, zone 3-HR above RCP. Firefighting performance was determined by a simple time-strain-air depletion model (TSA) taking the sum of z-transformed parameters of time to finish the exercise, strain in terms of mean heart rate, and air depletion from the breathing apparatus. Correlations were then established between TSA based firefighting performance parameters and fitness variables representing strength and endurance. Results: HR was significantly lower during SFE (79.9 ± 6.9%HRmax) compared to FOT (85.4 ± 5.2%HRmax). During SFE subjects spent 24.6 ± 30.2% of time in zone 1, 65.8 ± 28.1% in zone 2 and 9.7 ± 16.6% in zone 3. During FOT subjects spent 16.3 ± 12.8% in zone 1, 50.4 ± 13.2% in zone 2 and 33.3 ± 16.6% in zone 3. Out of all correlations, relative VO2peak showed the highest relation to mean HR during SFE (-0.593) as well as FOT (-0.693). Conclusions: Endurance in terms of VO2peak is an important prerequisite for both firefighting exercises. However, for standard simulated firefighting exercises it is important to work below VT1. For firefighting exercises in extreme temperatures with smoke, poor visibility and unexpected flashovers a high fitness level is required in order to keep the time spent above RCP as short as possible.