Assuntos
Paralisia Facial/diagnóstico , Herpes Zoster da Orelha Externa/diagnóstico , Vertigem/etiologia , Vômito/etiologia , Aciclovir/efeitos adversos , Aciclovir/uso terapêutico , Idoso de 80 Anos ou mais , Antivirais/efeitos adversos , Antivirais/uso terapêutico , Bromodesoxiuridina/análogos & derivados , Bromodesoxiuridina/uso terapêutico , Diagnóstico Diferencial , Paralisia Facial/tratamento farmacológico , Feminino , Herpes Zoster da Orelha Externa/tratamento farmacológico , Humanos , Resultado do TratamentoRESUMO
AIM: To determine levels of secretory IgA (sIgA), free secretory component (FSC) and IgD in saliva of newborn infants at the age of 1 day and to evaluate the detection patterns, the influence of saliva flow and the relation to serum derived proteins. METHODS: Seventy-three healthy newborn infants were studied. Saliva was obtained from the bottom of the mouth and buccal sulci using a sterile polyethylene tube connected to a syringe. SIgA, FSC, IgD and albumin were measured by radial immunodiffusion. RESULTS: SIgA was detected in 74.0% of all saliva samples, whereas detection rates for FSC and IgD were 94.5% and 75.3%, respectively. Investigation of detection patterns and their relation to saliva flow indicated that secretion of sIgA and FSC into the oral cavity is under similar regulation. Levels of IgD were found to be independent from saliva flow, as well as from concentrations of serum-derived proteins suggesting different regulative mechanisms compared to sIgA and FSC. The flow rate of unstimulated whole saliva in newborn infants was found to be 15 times lower compared to adolescents, emphasizing the role of saliva flow as a limiting factor for secretion of sIgA and FSC. CONCLUSION: SIgA, FSC and IgD can be determined in saliva of newborn infants even in the first day of life. The saliva flow rate has to be considered when evaluating the function and biological relevance of the oral mucosal immune system of newborn infants shortly after birth.
Assuntos
Imunoglobulina A Secretora/análise , Imunoglobulina D/análise , Recém-Nascido/fisiologia , Saliva/química , Componente Secretório/análise , Estudos Transversais , Feminino , Humanos , Masculino , Glândulas Salivares/imunologia , Glândulas Salivares/metabolismoRESUMO
As a first line of defence against microbial invasion, secretory IgA (sIgA) is the dominant immunoglobulin on all mucosal surfaces. In this study sIgA was determined by radial immunodiffusion in saliva samples of 63 newborn infants divided into the following age groups: (1) 1 day and younger, (2) 2-10 days. Concentrations of sIgA and albumin as well as their relation to age, postprandial time, gestational age and birth weight were analysed. sIgA could be detected in 75.0% (group 1) and 77.1% (group 2) of the saliva samples with a mean concentration of 190.2 mg/l (group 1) and 216.4 mg/l (group 2). Differences failed to reach significance. Concentration of sIgA was found to be independent of age but positively related to the concentration of albumin in the same saliva sample. In conclusion, this study provides evidence that high levels of sIgA are found in saliva of newborn infants, indicating the existence of a competent oral mucosal immune system as early as within the first 10 days of life.
