[Microdialysis study of release of adenine nucleotide breakdown products into the intercellular space of the myocardium in ischemia and reperfusion]. / Mikrodializnoe issledovanie vysvobozhdeniia v mezhkletochnoe prostranstvo miokarda produktov raspada adeninnukleotidov pri ishemii i reperfuzii.

Interstitial concentrations of adenine nucleotide breakdown products (ANBP)--adenosine (ADS), inosine (INS), hypoxanthine (HYP), in perfused rat hearts were measured during total ischemia and reperfusion by microdialysis technique. Progressive accumulation of ANBP in the interstitial space was observed during ischemia, but unlike INS and HYP, maximal ADS concentration was reached not by the end of ischemia but after the beginning of reperfusion. During reperfusion the qualitative accordance between changes in ANBP levels in the interstitial space and venous effluent was observed, but interstitial concentrations were several times greater.

[Selective activation of adrenaline secretion by the rat adrenal in neuroglycopenia detected via microdialysis]. / Izbiratel'naia aktivatsiia sekretsii adrenalina nadpochechnikom krysy pri neiroglikopenii, obnaruzhivaemaia s pomoshch'iu mikrodializa.

Secretion of adrenaline (A) and noradrenaline (NA) was measured in conscious Wistar rats using the microdialysis probe implanted into the adrenal gland. Changes in contents of A and NA in plasma as well as in dialysate samples in response to i. v. administration of 500 mg/kg 2-deoxy-D-glucose (2-DG) were measured by HPLC with electrochemical detection. 2-DG-induced neuroglycopenia led to a selective increase in adrenal secretion of A which reached maximum 20 m after 2-DG administration, whereas an increase in A and NA contents occurred in plasma. Elevation in the plasma NA content seems to reflect an enhancement in sympathetic nervous activity.

[A microdialysis study of catecholamine secretion in the rat adrenal gland during hypovolemic hypotension]. / Mikrodializnoe issledovanie sekretsii katekholaminov v nadpochechnike krysy pri gipovoliumicheskoi gipotenzii.

In anesthetized rats, concentrations of adrenaline and noradrenaline in sequential 20-min microdialysis samples were measured. Central activation of adrenal secretory function induced by controlled bleeding, increased catecholamine levels in dialysate but the maximal levels were reached after hemorrhage when a decrease in the blood pressure had been stabilized. The results of parallel measurements of catecholamine concentration in dialysate and blood samples suggest that reflex endocrine response of the adrenal gland during hypovolemic hypotension significantly exceeds sympathetic neural response.

[Study of catecholamine secretion by the rat adrenal glands using microdialysis in vivo]. / Issledovanie sekretsii katekholaminov v nadpochechnike krysy s pomoshch'iu mikrodializa in vivo.

Microdialysis technique has been developed to study secretory function of the adrenal gland in anesthesized rats. Concentration of adrenaline and noradrenaline in sequential 20 min microdialysis samples was measured by HPLC with electrochemical detection. The suitability of method was tested by local and central stimulation of catecholamine secretion. In the first case 100 mmol of KCl or 1 mmol of carbachol were added to perfusion medium, in the second one hypovolemic hypotension was applied. All the stimuli used increased catecholamine levels in the adrenal gland dialysates. Institute of Experimental Cardiology of the All-Union.