DNA, the biopolymer as a target material for metalloinsertors: From chemistry to preclinical implications.

The coordination of therapeutically interesting designed complexes of stoichiometry [ML(Met)Cl2] [where M=Cu(II), Co(II), Ni(II), Mn(II) and Zn(II), L=benzylidene-4-aminoantipyrine and Met=methionine] has been ascertained on the basis of physicochemical techniques. Their interaction with CT DNA reveals that they are good intercalators. The anticancer mechanism of our complexes is documented from their enhanced DNA splitting personalities under physiological conditions. To reveal the chemotherapeutic action of these complexes, we explored the inflammatory response, analgesic and antioxidant activities. Moreover, all the complexes show good antimicrobial activity against few bacterial and fungal strains. Our study has identified the mechanism of action of these complexes on inhibiting tumor cells and suggested that they have great potential as novel anticancer agents.

DNA binding mode of novel tetradentate amino acid based 2-hydroxybenzylidene-4-aminoantipyrine complexes.

Few transition metal complexes of tetradentate N(2)O(2) donor Schiff base ligands containing 2-hydroxybenzylidene-4-aminoantipyrine and amino acids (alanine/valine) abbreviated to KHL(1)/KHL(2) have been synthesized. All the metal complexes have been fully characterized with the help of elemental analyses, molecular weights, molar conductance values, magnetic moments and spectroscopic data. The Schiff bases KHL(1)/KHL(2) are found to act as tetradentate ligands using N(2)O(2) donor set of atoms leading to a square-planar geometry for the complexes around the metal ions. The binding behaviors of the complexes to calf thymus DNA have been investigated by absorption spectra, viscosity measurements and cyclic voltammetry. The DNA binding constants reveal that all these complexes interact with DNA through minor groove binding mode. The studies on mechanism of photocleavage reveal that singlet oxygen ((1)O(2)) and superoxide anion radical (O(2)(-)) may play an important role in the photocleavage. The Schiff bases and their metal complexes have been screened for their in vitro antibacterial activities against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, Klebsiella pneumoniae and antifungal activities against Aspergillus niger, Fusarium solani, Culvularia lunata, Rhizoctonia bataicola and Candida albicans by MIC method.