RESUMO
In recent years, low-density polyethylene (LDPE) has emerged as an essential component of the routine tasks that people engage in on a daily basis. However, over use of it resulted in environmental buildup that contaminated aquatic habitats and human health. Biodegradation is the most effective way for controlling pollution caused by synthetic plastic waste in a sustainable manner. In the present study, the LDPE degrading bacterial strain was screened from gut of Earthworms collected from plastic waste dumped area Mettur dam, Salem district, Tamil Nadu, India. The LDPE degrading bacterial strain was screened and identified genotypically. The LDPE degrading Bacillus gaemokensis strain SSR01 was submitted in NCBI. The B. gaemokensis strain SSR01 bacterial isolate degraded LDPE film after 14 days of incubation and demonstrated maximum weight loss of up to 4.98%. The study of deteriorated film using attenuated total reflection-Fourier transform infrared revealed the presence of a degraded product. The degradation of LDPE film by B. gaemokensis strain SSR01 was characterized by field-emission scanning electron microscopy analysis for surface alterations. The energy dispersive X-ray spectroscopy test confirmed that the broken-down LDPE film had basic carbon reduction. The present study of LDPE flim biodegradation by B. gaemokensis strain SSR01 has acted as a suitable candidate and will help in decreasing plastic waste.
Assuntos
Bacillus , Oligoquetos , Humanos , Animais , Polietileno , Índia , Biodegradação AmbientalRESUMO
AIMS: The lignocellulosic waste, Borassus flabellifer empty fruit bunch waste (BFEFBW), was employed to produce laccase using Bacillus aestuarii KSK under solid-state fermentation (SSF) conditions and to assess the efficiency of malachite green (MG) dye decolourization. METHODS AND RESULTS: Abiotic factors such as pH (5.0-9.0), temperature (25-45°C) and incubation time (24-96 h) were optimized using Response surface methodology-Box-Behenan Design (RSM-BBD) to exploit the laccase production. The anticipated model revealed that the highest laccase activity of 437 U/ml shows after 60 h of incubation at 35°C at pH 7.0. The bacterial laccase was used to remove 89% of the MG dye in less time. CONCLUSION: The laccase from B. aestuarii KSK decolorizes the MG and thereby making it a suitable choice for wastewater treatment from industrial effluents. SIGNIFICANCE AND IMPACT OF THE STUDY: This study is the first report on the production of laccase from B. flabellifer empty fruit bunch waste as a substrate. Bacillus aestuarii KSK was isolated from the soil sample and used to produce laccase under SSF conditions. The bacterial laccase has the potential for industrial application in textile waste dye treatment.
Assuntos
Arecaceae , Lacase , Lacase/metabolismo , Frutas/metabolismo , Corantes/metabolismo , Arecaceae/metabolismo , Biodegradação AmbientalRESUMO
The present study focused to synthesize the copper oxide nanoparticles (CuONPs) using novel Canthium coromandelicum leaves in a cost-effective, easy, and sustainable approach. The obtained Canthium coromandelicum-copper oxide nanoparticles (CC-CuONPs) were characterized using UV-Visible spectroscopy, FT-IR analysis, FESEM, HR-TEM imaging, and XRD study. The XRD pattern verified the development of crystalline CC-CuONPs with an average size of 33 nm. The biosynthesized CC-CuONPs were roughly spherical, according to HR-TEM and FESEM analyses. FT-IR research verified the existence of functional groups involved in CC-CuONPs production. Cu and O2 have high-energy signals of 78.32% and 12.78%, respectively, according to data from EDX. The photocatalytic evaluation showed that synthesized CC-CuONPs have the efficiency of degrading methylene blue (MB) and methyl orange (MO) by 91.32%, 89.35% respectively. The findings showed that biosynthesized CC-CuONPs might effectively remove contaminants in an environmentally acceptable manner.
Assuntos
Nanopartículas Metálicas , Nanopartículas , Antibacterianos/química , Corantes , Cobre/química , Química Verde/métodos , Nanopartículas Metálicas/química , Nanopartículas/química , Óxidos , Extratos Vegetais , Folhas de Planta , Espectroscopia de Infravermelho com Transformada de Fourier , TêxteisRESUMO
The present investigation reports a simple, rapid, inexpensive, and eco-friendly approach for synthesizing Amphiroa rigida-mediated silver nanoparticles (AR-AgNPs) for the first time. The biomimetic synthesized AR-AgNPs were characterized by both spectral and microscopic analysis. The UV-visible spectrum showed the surface plasmon peak at 420 nm, which indicated the formation of AR-AgNPs. X-ray diffraction characterization of AR-AgNPs showed a face-centered cubic crystal (25 nm) and the transmission electron microscope micrograph showed spherical shape. The functional group of polysaccharide that plays a major role as a stabilizing and reducing agent is confirmed by Fourier-transform infrared spectroscopy. The biomimetic synthesized AR-AgNPs showed antibacterial activity against Staphylococcus aureus (21 ± 0.2 mm) and Pseudomonas aeruginosa (15 ± 0.2 mm). Further, the cytotoxic effects of AR-AgNPs against MCF-7 human breast cancer cells were observed through acridine orange-ethidium bromide and Hoechst staining. Besides, AR-AgNPs are found to be inhibit the growth of 3rd and 4th instar larvae of Aedes aegypti in a dose-dependent manner.
Assuntos
Aedes/crescimento & desenvolvimento , Antibacterianos , Materiais Biomiméticos , Citotoxinas , Inseticidas , Nanopartículas Metálicas , Pseudomonas aeruginosa/crescimento & desenvolvimento , Rodófitas/química , Prata , Staphylococcus aureus/crescimento & desenvolvimento , Animais , Antibacterianos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Materiais Biomiméticos/síntese química , Materiais Biomiméticos/química , Materiais Biomiméticos/farmacologia , Citotoxinas/síntese química , Citotoxinas/química , Citotoxinas/farmacologia , Química Verde , Humanos , Inseticidas/síntese química , Inseticidas/química , Inseticidas/farmacologia , Larva/crescimento & desenvolvimento , Células MCF-7 , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Prata/química , Prata/farmacologiaRESUMO
A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1-4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 µg/mL.
Assuntos
Antibacterianos , Helianthus/química , Nanopartículas Metálicas , Extratos Vegetais , Prata , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antibacterianos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Nanopartículas Metálicas/química , Nanopartículas Metálicas/toxicidade , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/toxicidade , Ralstonia solanacearum/efeitos dos fármacos , Ratos , Prata/química , Prata/farmacologia , Prata/toxicidade , Baço/citologia , Xanthomonas axonopodis/efeitos dos fármacosRESUMO
Silver nanoparticle (AgNP) synthesis and characterization is an area of vast interest due to their broader application in the fields of science and technology and medicine. Plants are an attractive source for AgNP synthesis because of its ability to produce a wide range of secondary metabolites with strong reducing potentials. Thus, the present study describes the synthesis of AgNPs using aqueous rhizome extract of Acorus calamus (sweet flag). The AgNP formation was evaluated at different temperatures, incubation time and concentrations of AgNO3 using Response surface methodology based Box-Behnken design (BBD). The synthesized AgNPs were characterized by UV-Visible spectroscopy, Fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), and Scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS). The surface plasmon resonance found at 420 nm confirmed the formation of AgNPs. SEM images reveal that the particles are spherical in nature. The EDS analysis of the AgNPs, using an energy range of 2-4 keV, confirmed the presence of elemental silver without any contamination. The antibacterial activity of synthesized AgNPs was evaluated against the clinical isolates Staphylococcus aureus and Escherichia coli and it was found that bacterial growth was significantly inhibited in a dose dependent manner. The results suggest that the AgNPs from rhizome extract could be used as a potential antibacterial agent for commercial application.