RESUMO
Human pregnancy-associated plasma protein A (PAPP-A) inhibited significantly the proteolytic activity of bovine trypsin and human plasmin. Trypsin or plasmin treatment of PAPP-A resulted in the generation of a major 85 kDa component and the rapid cleavage of internal thiol esters. The results indicated that both of these serine proteinases bound in a 1:1 stoichiometry to PAPP-A. The PAPP-A-bound enzymes were found to be enzymatically active towards small synthetic substrates and inaccessible to inactivation by soybean trypsin inhibitor and alpha 1-proteinase inhibitor. The mechanism of proteinase inhibition was likely to be entrapment, as described for alpha 2-macroglobulin.
Assuntos
Proteína Plasmática A Associada à Gravidez/farmacologia , Inibidores de Proteases/farmacologia , Sequência de Aminoácidos , Animais , Bovinos , Eletroforese em Gel de Poliacrilamida , Fibrinolisina/metabolismo , Humanos , Dados de Sequência Molecular , Proteínas de Plantas/farmacologia , Proteína Plasmática A Associada à Gravidez/metabolismo , Inibidores de Serina Proteinase/farmacologia , Tripsina/metabolismo , Inibidores da Tripsina , alfa 1-Antitripsina/farmacologia , alfa-Amilases/antagonistas & inibidoresRESUMO
The paper reviews the problems of professional medical orientation in Russia and abroad. Different opinions on occupational orientation of young people with regard to medicine are presented and the attitude to the profession of a nurse, among other things, is analyzed. Positive results attained in the sphere of professional orientation and the shortcomings are analyzed. The author emphasizes that the problem is still to be researched and new methods should be developed to solve it.
