[Normal parameters of leukocyte index of intoxication]. / Nekotorye dannye o pokazateliakh normy leikotsitarnogo indeksa intoksikatsii.

A formula was suggested to determine the leukocytic index of intoxication (LII), which is a correlation between the level of neutrophils and the content of other cells in the blood leukocytic composition. The above formula was used to estimate the minimal, maximal and mean LII indices in patients, who had no inflammatory or purulent pathologies, i.e. normal indices, which respectively amounted to 1, 3 and 1.9. Such approach to composing a formula is in line with the specific features of vital activity, unlike Ya.Ya. Kalf-Kalif formula, which (when it was composed) it was added a number of coefficients. The application of the offered formula to determine the LII at acute appendicitis showed that the more severe an acute appendicitis was so much the LII increase was more intensive. It is noteworthy, that the high LII indices did not always correlate with an increased quantity of blood leukocytes. The above stated proves that the offered LII formula can be used in practice.

[Combined effect of laser radiation and alternating magnetic fields on Staphylococci]. / Kombinirovannoe vliianie lazernykh izluchenii i peremennykh magnitnykh polei na stafilokokki.

The influence of infrared and helio-neon lasers, alternate magnetic fields and their combination on the growth phases of staphylococci was studied. The study revealed that in most cases the inhibition of the growth phases of staphylococci was caused by the treatment of staphylococcal cultures with alternate magnetic fields in combination with infrared laser irradiation. The most effective treatment conditions were found.

Speciation studies by capillary electrophoresis- Simultaneous determination of chromium(III) and chromium(VI).

A method for the simultaneous determination of chromium(III) and chromium(VI) by capillary electrophoresis (CE) has been developed. The chromium(III) has been chelated with 1,2-cyclohexanediaminetetraacetic acid (CDTA) in order to impart a negative charge and similar mobility to both the chromium(III) and the chromium(VI) species. The effects of the amount of the reagent, pH and heating time required to complete the complexation have been studied. Factors affecting the CE behaviour such as the polarity of electrodes and the pH of electrophoretic buffer have been investigated. The separated species have been monitored by direct UV measurements at 214 nm. The detection limits achieved are 10 microg/l for Cr(VI) and 5 microg/l for Cr(III) and linear detector response is observed up to 100 mg/l. The procedure has been applied to the determination of both chromium species in industrial electroplating samples and its accuracy was checked by comparing the results (as total chromium) with those of atomic absorption spectrometry. No interference occurred from transition metal impurities under optimized separation conditions. The method is also shown to be feasible for determining Cr(III) as well as other metal ions capable to form complexes with CDTA (like iron(III), copper(II), zinc(II) and manganese(II)) in pharmaceutical preparations of essential trace elements.

Stability study of novel zinc complexes of pharmaceutical relevance by CE.

CE was used as a stability-indicating assay for zinc mixed-ligand complexes with promised biological activity. CE measurements indicated that Zn(RCOO)2Ln compounds (where R = H, CH3, C2H5, or C3H7; L = caffeine, nicotinic acid, thiourea, or phenazone; and n = 1 or 2) in water tend to decompose at room temperature. For thiourea and phenazone compounds, the degradation products (free zinc ion, carboxylate anion, and the corresponding neutral ligand) were found to increase within a few hours of storage in water. More stable complexes of caffeine and nicotinic acid showed no appreciable changes in CE behavior during the same period of time and only decomposed notably after 10 hr. The stability differences of zinc complexes were characterized in terms of apparent half-life values and rate constants of decomposition reaction. Relationships between the stability and structure of the complexes are discussed, and theoretical interpretation is presented.