Opposing tumor-cell-intrinsic and -extrinsic roles of the IRF1 transcription factor in antitumor immunity.

Type I interferon (IFN-I) and IFN-γ foster antitumor immunity by facilitating T cell responses. Paradoxically, IFNs may promote T cell exhaustion by activating immune checkpoints. The downstream regulators of these disparate responses are incompletely understood. Here, we describe how interferon regulatory factor 1 (IRF1) orchestrates these opposing effects of IFNs. IRF1 expression in tumor cells blocks Toll-like receptor- and IFN-I-dependent host antitumor immunity by preventing interferon-stimulated gene (ISG) and effector programs in immune cells. In contrast, expression of IRF1 in the host is required for antitumor immunity. Mechanistically, IRF1 binds distinctly or together with STAT1 at promoters of immunosuppressive but not immunostimulatory ISGs in tumor cells. Overexpression of programmed cell death ligand 1 (PD-L1) in Irf1-/- tumors only partially restores tumor growth, suggesting multifactorial effects of IRF1 on antitumor immunity. Thus, we identify that IRF1 expression in tumor cells opposes host IFN-I- and IRF1-dependent antitumor immunity to facilitate immune escape and tumor growth.

Hierarchical Capillarity-Assisted Liquid Invasion in Multilayered Paper Channels for Nanoelectrokinetic Preconcentration.

A nanoelectrokinetic phenomenon called ion concentration polarization (ICP) has been recently applied to microfluidic paper-based devices for the high fold preconcentration of low-abundant analytes. The inherent microstructural characteristics of cellulose papers can sufficiently stabilize the chaotic electroconvection of ICP, which is a significant annoyance for typical engineered microfluidic channels. However, a high electrical voltage to induce ICP in a paper-fluidic channel can increase unavoidable electrophoretic forces over drag forces so that the preconcentrated plug is rapidly receded with severe dispersion. In order to enhance the hydraulic drag force that helps the preconcentration of analytes, here we introduce a multilayered paper structure into paper-fluidic channel. We theoretically and experimentally demonstrate that a hierarchical capillary structure in a multilayered paper-fluidic channel can effectively increase the hydraulic drag force. For the practical utility in the field of diagnostics, the mechanism is verified by a simple example of the immunoassay using biotin-streptavidin complexation.

PbS Quantum Dots-Decorated BiVO4 Photoanodes for Highly Efficient Photoelectrochemical Hydrogen Production.

While metal oxides such as TiO2, Fe2O3, WO3, and BiVO4 have been previously studied for their potential as photoanodes in photoelectrochemical (PEC) hydrogen production, their relatively wide band-gap limits their photocurrent, making them unsuitable for the efficient utilization of incident visible light. To overcome this limitation, we propose a new approach for highly efficient PEC hydrogen production based on a novel photoanode composed of BiVO4/PbS quantum dots (QDs). Crystallized monoclinic BiVO4 films were prepared via a typical electrodeposition process, followed by the deposition of PbS QDs using a successive ionic layer adsorption and reaction (SILAR) method to form a p-n heterojunction. This is the first time that narrow band-gap QDs were applied to sensitize a BiVO4 photoelectrode. The PbS QDs were uniformly coated on the surface of nanoporous BiVO4, and their optical band-gap was reduced by increasing the number of SILAR cycles. However, this did not affect the crystal structure and optical properties of the BiVO4. By decorating the surface of BiVO4 with PbS QDs, the photocurrent was increased from 2.92 to 4.88 mA/cm2 (at 1.23 VRHE) for PEC hydrogen production, resulting from the enhanced light-harvesting capability arising from the narrow band-gap of the PbS QDs. Moreover, the introduction of a ZnS overlayer on the BiVO4/PbS QDs further improved the photocurrent to 5.19 mA/cm2, attributed to the reduction in interfacial charge recombination.

Investigating the Influence of PbS Quantum Dot-Decorated TiO2 Photoanode Thickness on Photoelectrochemical Hydrogen Production Performance.

To maximize the photoelectrochemical (PEC) hydrogen production performance of quantum dot (QD)-decorated photoelectrodes, it is crucial to prioritize the optimization of electrode's structure, including thickness and porosity. In this study, we prepare PbS QD-decorated mesoporous TiO2 photoanodes for PEC hydrogen production, and systematically investigate the influence of the photoanode thickness on optical properties and PEC performances. As the thickness of photoanodes increases from 6.4 µm to 16.3 µm, the light absorption capability is enhanced across the entire visible and near-infrared (IR) spectrum due to the improved loading of PbS QDs. However, the photocurrent density is optimized for the 11.9 µm thick photoanode (15.19 mA/cm2), compared to the 6.4 µm thick (10.80 mA/cm2) and 16.3 µm thick photoanodes (11.93 mA/cm2). This optimization is attributed to the trade-off between the light absorption capability and the efficient mass transfer of the electrolyte as the photoanode thickness increases, which is confirmed by the lowest charge transfer resistance (Rct) evaluated from the electrochemical impedance data.

Rapid and accurate nanoelectrokinetic diagnosis of drug-resistant bacteria.

Increased antimicrobial resistance presents a major threat to public health, and it is a global health problem due to the rapid globalization and transmission of infectious diseases. However, fast and precise diagnosis tool is lacking, and inappropriate antibiotic prescription leads to the unforeseen production of drug-resistant bacteria. Here, we report a Rapid and Accurate Nanoelectrokinetic Diagnostic System (RANDx) for detecting drug-resistant bacteria, which cause a common infectious disease called Urinary Tract Infection (UTI), within 7 min. We develop nanoelectrokinetic paper-based analytic device (NEK-PAD) as a sample prep module of RANDx and obtain >100-fold post-wetting preconcentration by balancing between ion concentration polarization (ICP) and radial imbibition for a constant flow rate. Simultaneously with preconcentration, our cathodic nanochannel design enables NEK-PAD to extract drug-resistant enzymes without denaturation and accelerate enzyme-linked reactions under electrical spontaneous heating at approximately 37 °C. Finally, using a cell phone camera, we detect label-free drug-resistant bacteria as low as 104 cfu/mL, which is higher than clinically required threshold (>105 cfu/mL) by enhancing 1000 times of the limit of detection (LOD) of colorimetric nitrocefin assay. We believe that the RANDx will be an innovative precision medicine tool for UTI and other infectious diseases in limited remote settings.

Electrokinetic-Driven Fast Ion Delivery for Reversible Aqueous Zinc Metal Batteries with High Capacity.

Aqueous zinc (Zn) metal batteries (ZMBs) are considered a promising candidate for grid-scale energy storage due to their freedom from fire hazards. However, a limited reversibility of Zn metal electrode caused by dendritic Zn growth has hindered the advent of high-capacity Zn metal batteries (>4 mAh cm-2 ). Herein, it is reported that fast electrokinetic Zn-ion transport extremely improves the Zn metal reversibility. It is revealed that a negatively charged porous layer (NPL) provides the electrokinetic Zn-ion transport by surface conduction, and consequently impedes the depletion of Zn-ion on electrode surface as indicated by numerical simulations and overlimiting current behavior. Due to the quick Zn-ion delivery, a dendrite-free and densely packed Zn metal deposit is accommodated inside its pores. With the introduction of the NPL, the cycling stability of Zn symmetric cell is enhanced by 21 times at 10 mA cm-2 /10 mAh cm-2 . Average Coulombic efficiency of 99.6% is achieved over 500 cycles for electrodeposition/stripping at 30 mA cm-2 /5 mAh cm-2 on NPL-Cu electrode. Furthermore, a high-capacity Zn/V2 O5 full cell with the NPL exhibits an extraordinary stability over 1000 cycles at a capacity of 4.8 mAh cm-2 .

A Preliminary Study on the Effectiveness of the Peer Relationship Enhancement Program in Adolescents at Risk for Internet and Smartphone Addiction.

OBJECTIVES: The purpose of this study is to evaluate the preliminary effects of the Peer Relationship Enhancement Program in adolescents deemed to be in an at-risk group for Internet and smartphone addiction. METHODS: The study group consisted of 33 adolescent participants (24 boys and 9 girls) at risk of Internet and smartphone addiction in small and medium-sized cities. The subjects participated in 8 consecutive sessions of the Peer Relationship Enhancement Program. The Korean Internet Addiction Proneness Scale, the Korean Smartphone Addiction Proneness Scale, the Real-Ideal Self Discrepancy Scale, the UCLA Loneliness Scale, the Peer Intimacy Scale, and the Escaping from the Self Scale were evaluated before the initial and after the final session. A paired t-test was performed to statistically analyze the data. RESULTS: The Peer Relationship Enhancement Program led to a significant decrease (p<0.05) in self-reported measures of The Korean Internet Addiction Proneness Scale, the Korean Smartphone Addiction Proneness Scale, and the Real-Ideal Self Discrepancy Scale. CONCLUSION: The Peer Relationship Enhancement Program reduces the risk of Internet and smartphone addiction and effectively prevents the associated problems.