Innovative tailor made dextran based membranes with excellent non-inflammatory response: In vivo assessment.

In this work, dextran based membranes with potential to be used as implantable devices in Tissue Engineering and Regenerative Medicine (TERM) were prepared by a straightforward strategy. Briefly, two polymers approved by the Food and Drug Administration, viz. dextran and poly(ε-caprolactone) (PCL) were functionalized with methacrylate moieties, and subjected to photocrosslinking. Employing different weight ratios of each polymer in the formulations allowed to obtain transparent membranes with tunable physicochemical properties and low adverse host tissue response. Independently of the material, all formulations have shown to be thermally stable up to 300 °C whilst variations in the polymer ratio resulted in membranes with different glass transition temperatures (Tg) and flexibility. The swelling capacity ranged from 50% to 200%. On the other hand, in vitro hydrolytic degradation did not show to be material-dependent and all membranes maintained their structural integrity for more than 30 days, losing only 8-12% of their initial weight. Preliminary in vitro biological tests did not show any cytotoxic effect on seeded human dental pulp stem cells (hDPSCs), suggesting that, in general, all membranes are capable of supporting cell adhesion and viability. The in vivo biocompatibility of membranes implanted subcutaneously in rats' dorsum indicate that M100/0 (100%wt dextran) and M25/75 (25 %wt dextran) formulations can be classified as "slight-irritant" and "non-irritant", respectively. From the histological analysis performed on the main tissue organs it was not possible to detect any signs of fibrosis or necrosis thereby excluding the presence of toxic degradation by-products deposited or accumulated in these tissues. In combination, these results suggest that the newly developed formulations hold great potential as engineered devices for biomedical applications, where the biological response of cells and tissues are greatly dependent on the physical and chemical cues provided by the substrate.

Unexpectedly high genetic diversity and divergence among populations of the apomictic Neotropical tree Miconia albicans.

Since tropical trees often have long generation times and relatively small reproductive populations, breeding systems and genetic variation are important for population viability and have consequences for conservation. Miconia albicans is an obligate, diplosporous, apomictic species widespread in the Brazilian Cerrado, the savanna areas in central Brazil and elsewhere in the Neotropics. The genetic variability would be, theoretically, low within these male-sterile and possibly clonal populations, although some variation would be expected due to recombination during restitutional meiosis. We used ISSR markers to assess genetic diversity of M. albicans and to compare with other tropical trees, including invasive species of Melastomataceae. A total of 120 individuals from six populations were analysed using ten ISSR primers, which produced 153 fully reproducible fragments. The populations of M. albicans presented mean Shannon's information index (I) of 0.244 and expected heterozygosity (He ) of 0.168. Only two pairs of apparently clonal trees were identified, and genetic diversity was relatively high. A hierarchical amova for all ISSR datasets showed that 74% of the variance was found among populations, while only 26% of the variance was found within populations of this species. Multivariate and Bayesian analyses indicated marked separation between the studied populations. The genetic diversity generated by restitutional meiosis, polyploidy and possibly other genome changes may explain the morpho-physiological plasticity and the ability of these plants to differentiate and occupy such a wide territory and different environmental conditions. Producing enormous amounts of bird-dispersed fruits, M. albicans possess weedy potential that may rival other Melastomataceae alien invaders.

An alternating direction algorithm for total variation reconstruction of distributed parameters.

Augmented Lagrangian variational formulations and alternating optimization have been adopted to solve distributed parameter estimation problems. The alternating direction method of multipliers (ADMM) is one of such formulations/optimization methods. Very recently, the number of applications of the ADMM, or variants of it, to solve inverse problems in image and signal processing has increased at an exponential rate. The reason for this interest is that ADMM decomposes a difficult optimization problem into a sequence of much simpler problems. In this paper, we use the ADMM to reconstruct piecewise-smooth distributed parameters of elliptical partial differential equations from noisy and linear (blurred) observations of the underlying field. The distributed parameters are estimated by solving an inverse problem with total variation (TV) regularization. The proposed instance of the ADMM solves, in each iteration, an l(2) and a decoupled l(2) - l(1) optimization problems. An operator splitting is used to simplify the treatment of the TV regularizer, avoiding its smooth approximation and yielding a simple yet effective ADMM reconstruction method compared with previously proposed approaches. The competitiveness of the proposed method, with respect to the state-of-the-art, is illustrated in simulated 1-D and 2-D elliptical equation problems, which are representative of many real applications.

Halogen atom effect on photophysical and photodynamic characteristics of derivatives of 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin.

Brominated and iodinated derivatives of 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin were synthesised directly from the corresponding aldehydes. Photophysical and photochemical properties, singlet oxygen formation quantum yields, photobleaching and log P were measured. Cellular uptake measurements and cytotoxicity assays on WiDr and A375 tumour cell lines were performed. 5,10,15,20-Tetrakis(2-bromo-5-hydroxyphenyl)porphyrin showed the best cytotoxicity with values of IC(50) of 113 nM over WiDr cells and 52nM over A375 cells.

3-Hydroxybenzaldehyde.

The title compound, C(7)H(6)O(2), forms infinite chains where the molecules are hydrogen bonded via the hydroxyl and aldehyde groups, with an O.O distance of 2.719 (3) A. Interchain interactions are weak. The geometry of the ring differs from the ideal form due to the effect of the substituents. Ab initio (Hartree-Fock self-consistent field-molecular orbital and density functional theory) calculations for the free molecule reproduce well the observed small distortions of the ring. In the crystal, the geometry deviates from the ideal C(s) symmetry of the free molecule, as given by the ab initio calculations. The aldehyde and hydroxyl groups are twisted around the single bonds which join them to the ring as a result of the intermolecular hydrogen-bond interactions. These are also responsible for an elongation of the hydroxy C-OH bond compared with that calculated for the free molecule.