Towards standardisation of surface electromyography measurements in the horse: Bipolar electrode location.

The use of surface electromyography in the field of animal locomotion has increased considerably over the past decade. However, no consensus exists on the methodology for data collection in horses. This study aimed to start the development of recommendations for bipolar electrode locations to collect surface electromyographic data from horses during dynamic tasks. Data were collected from 21 superficial muscles of three horses during trot on a treadmill using linear electrode arrays. The data were assessed both quantitatively (signal-to-noise ratio (SNR) and coefficient of variation (CoV)) and qualitatively (presence of crosstalk and activation patterns) to compare and select electrode locations for each muscle. For most muscles and horses, the highest SNR values were detected near or cranial/proximal to the central region of the muscle. Concerning the CoV, there were larger differences between muscles and horses than within muscles. Qualitatively, crosstalk was suspected to be present in the signals of twelve muscles but not in all locations in the arrays. With this study, a first attempt is made to develop recommendations for bipolar electrode locations for muscle activity measurements during dynamic contractions in horses. The results may help to improve the reliability and reproducibility of study results in equine biomechanics.

Normal variation in pelvic roll motion pattern during straight-line trot in hand in warmblood horses.

In horses, hip hike asymmetry, i.e. left-right difference in hip upwards movement during hind limb protraction in trot, is a crucial lameness sign. Vertical hip movements are complex, influenced by both pelvic roll and pelvic vertical motion. Veterinarians find it challenging to identify low-grade lameness, and knowledge of normal variation is a prerequisite for discerning abnormalities. This study, which included 100 clinically sound Warmblood horses, aimed to describe normal variation in pelvic roll stride patterns. Data were collected during straight-line trot in hand using optical motion capture. Stride-segmented pelvic roll data, normalised with respect to time (0-100% of the stride) and amplitude (± 0.5 of horse average stride range of motion), were modelled as a linear combination of sine and cosine curves. A sine curve with one period per stride and a cosine curve with three periods per stride explained the largest proportions of roll motion: model estimate 0.335 (p < 0.01) and 0.138 (p < 0.01), respectively. Using finite mixture models, the horses could be separated into three groups sharing common pelvic roll characteristics. In conclusion, pelvic roll motion in trot follows a similar basic pattern in most horses, yet there is significant individual variation in the relative prominence of the most characteristic features.

Reliability of surface electromyographic (sEMG) measures of equine axial and appendicular muscles during overground trot.

The reliability of surface electromyography (sEMG) has not been adequately demonstrated in the equine literature and is an essential consideration as a methodology for application in clinical gait analysis. This observational study investigated within-session, intra-subject (stride-to-stride) and inter-subject reliability, and between-session reliability of normalised sEMG activity profiles, from triceps brachii (triceps), latissimus dorsi (latissimus), longissimus dorsi (longissimus), biceps femoris (biceps), superficial gluteal (gluteal) and semitendinosus muscles in n = 8 clinically non-lame horses during in-hand trot. sEMG sensors were bilaterally located on muscles to collect data during two test sessions (session 1 and 2) with a minimum 24-hour interval. Raw sEMG signals from ten trot strides per horse and session were DC-offset removed, high-pass filtered (40 Hz), full-wave rectified, and low-pass filtered (25 Hz). Signals were normalised to peak amplitude and percent stride before calculating intra- and inter-subject ensemble average sEMG profiles across strides for each muscle and session. sEMG profiles were assessed using waveform similarity statistics: the coefficient of variation (CV) to assess intra- and inter-subject reliability and the adjusted coefficient of multiple correlation (CMC) to evaluate between-session reliability. Across muscles, CV data revealed that intra-horse sEMG profiles within- and between-sessions were comparatively more reliable than inter-horse profiles. Bilateral gluteal, semitendinosus, triceps and longissimus (at T14 and L1) and right biceps showed excellent between-session reliability with group-averaged CMCs > 0.90 (range 0.90-0.97). Bilateral latissimus and left biceps showed good between-session reliability with group-averaged CMCs > 0.75 (range 0.78-0.88). sEMG profiles can reliably describe fundamental muscle activity patterns for selected equine muscles within a test session for individual horses (intra-subject). However, these profiles are more variable across horses (inter-subject) and between sessions (between-session reliability), suggesting that it is reasonable to use sEMG to objectively monitor the intra-individual activity of these muscles across multiple gait evaluation sessions at in-hand trot.

Rater agreement for assessment of equine back mobility at walk and trot compared to quantitative gait analysis.

BACKGROUND: Lameness assessment in horses is still predominantly performed using subjective methods. Visual assessment is known to have moderate to good intra-rater agreement but relatively poor inter-rater agreement. Little is known about inter- and intra-rater agreement on the evaluation of back motion, for which no objective measurement technique in a clinical setting is available thus far. OBJECTIVES: To describe inter- and intra-rater agreement of visual evaluation of equine back mobility. STUDY DESIGN: Rater reliability study using a fully crossed design in which all horses are rated by all observers. This data is compared with objective gait analysis. METHODS: Seventy equine professionals (veterinarians and physiotherapists) and veterinary students evaluated videos of 12 healthy horses at walk and trot on a hard, straight line. Nine parameters related to back mobility were scored: general mobility, thoracic, lumbar, lumbosacral flexion and extension and left and right thoracolumbar latero-flexion. All parameters were compared with simultaneously measured quantitative motion parameters. After 1 month, six randomly chosen horses were re-evaluated by 57 observers. RESULTS: For each parameter inter- and intra-rater agreements were calculated using intra-class correlation coefficients. For all parameters, inter-rater agreement was very poor (<0.2). The mean intra-rater agreement of all observers and for all parameters was poor (~0.4) but varied between 0.0 and 0.96 for individual observers. There was no correlation between the visual subjective scoring and objective gait analysis measurements. MAIN LIMITATIONS: Horses were scored from videos and by lack of any existing (semi-) quantitative system, a custom-made system had to be used. CONCLUSIONS: The poor inter- and intra-rater agreements of visual scoring of mobility of the equine back and the disagreement between subjective and objective gait analysis data, demonstrate the need for the development and introduction of objective, quantitative and repeatable techniques to assess equine back motion.

Differences in equine spinal kinematics between straight line and circle in trot.

Work on curved tracks, e.g. on circles, is commonplace within all forms of horse training. Horse movements in circles are naturally asymmetric, including the load distribution between inner and outer limbs. Within equestrian dressage the horse is expected to bend the back laterally to follow the circle, but this has never been studied scientifically. In the current study 12 horses were measured (optical motion capture, 100 Hz) trotting on left and right circles and on the straight without rider (soft surface). Data from markers placed along the spine indicated increased lateral bending to the inside (e.g. left bending on the left circle) of the thoracolumbar back (difference left circle vs. straight - 3.75°; right circle + 3.61°) and the neck (left - 5.23°; right + 4.80° vs. straight). Lateral bending ROM increased on the circle (+ 0.87° and + 0.62°). Individual variation in straight-circle differences was evident, but each horse was generally consistent over multiple trials. Differences in back movements between circle and straight were generally small and may or may not be visible, but accompanying changes in muscle activity and limb movements may add to the visual impression.

Improving gait classification in horses by using inertial measurement unit (IMU) generated data and machine learning.

For centuries humans have been fascinated by the natural beauty of horses in motion and their different gaits. Gait classification (GC) is commonly performed through visual assessment and reliable, automated methods for real-time objective GC in horses are warranted. In this study, we used a full body network of wireless, high sampling-rate sensors combined with machine learning to fully automatically classify gait. Using data from 120 horses of four different domestic breeds, equipped with seven motion sensors, we included 7576 strides from eight different gaits. GC was trained using several machine-learning approaches, both from feature-extracted data and from raw sensor data. Our best GC model achieved 97% accuracy. Our technique facilitated accurate, GC that enables in-depth biomechanical studies and allows for highly accurate phenotyping of gait for genetic research and breeding. Our approach lends itself for potential use in other quadrupedal species without the need for developing gait/animal specific algorithms.

Range of motion and between-measurement variation of spinal kinematics in sound horses at trot on the straight line and on the lunge.

Clinical assessment of spinal motion in horses is part of many routine clinical exams but remains highly subjective. A prerequisite for the quantification of spinal motion is the assessment of the expected normal range of motion and variability of back kinematics. The aim of this study was to objectively quantify spinal kinematics and between -measurement, -surface and -day variation in owner-sound horses. In an observational study, twelve owner-sound horses were trotted 12 times on four different paths (hard/soft straight line, soft lunge left and right). Measurements were divided over three days, with five repetitions on day one and two, and two repetitions on day three (recheck) which occurred 28-55 days later. Optical motion capture was used to collect kinematic data. Elements of the outcome were: 1) Ranges of Motion (ROM) with confidence intervals per path and surface, 2) a variability model to calculate between-measurement variation and test the effect of time, surface and path, 3) intraclass correlation coefficients (ICC) to determine repeatability. ROM was lowest on the hard straight line. Cervical lateral bending was doubled on the left compared to the right lunge. Mean variation for the flexion-extension and lateral bending of the whole back were 0.8 and 1 degrees. Pelvic motion showed a variation of 1.0 (pitch), 0.7 (yaw) and 1.3 (roll) degrees. For these five parameters, a tendency for more variation on the hard surface and reduced variation with increased repetitions was observed. More variation was seen on the recheck (p<0.001). ICC values for pelvic rotations were between 0.76 and 0.93, for the whole back flexion-extension and lateral bending between 0.51 and 0.91. Between-horse variation was substantially higher than within-horse variation. In conclusion, ROM and variation in spinal biomechanics are horse-specific and small, necessitating individual analysis and making subjective and objective clinical assessment of spinal kinematics challenging.

Variation in gait parameters used for objective lameness assessment in sound horses at the trot on the straight line and the lunge.

BACKGROUND: Objective lameness assessment is gaining more importance in a clinical setting, necessitating availability of reference values. OBJECTIVES: To investigate the between -path, -trial and -day variation, between and within horses, in the locomotion symmetry of horses in regular use that are perceived sound. STUDY DESIGN: Observational study with replicated measurement sessions. METHODS: Twelve owner-sound horses were trotted on the straight line and on the lunge. Kinematic data were collected from these horses using 3D optical motion capture. Examinations were repeated on 12 occasions over the study which lasted 42 days in total. For each horse, measurements were grouped as five replicates on the first and second measurement days and two replicates on the third measurement day. Between measurement days 2 and 3, every horse had a break from examination of at least 28 days. Previously described symmetry parameters were calculated: RUD and RDD (Range Up/Down Difference; difference in upward/downward movement between right and left halves of a stride); MinDiff and MaxDiff (difference between the two minima/maxima of the movement); HHDswing and HHDstance (Hip Hike Difference-swing/-stance; difference between the upward movement of the tuber coxae during swingphase/stancephase). Data are described by the between-measurement variation for each parameter. A linear mixed model was used to test for the effect of time, surface and path. Intraclass correlation coefficients (ICC) were calculated to access repeatability. RESULTS: Mean between-measurement variation was (MinDiff, MaxDiff, RUD, RDD): 13, 12, 20, 16 mm (head); 4, 3, 6, 4 mm (withers) and 5, 4, 6, 6 mm (pelvis); (HHDswing, HHDstance): 7 and 7 mm. More between-measurement variation is seen on the first measurement day compared to the second and third measurement days. In general, less variation is seen with increasing number of repetitions. Less between-measurement variation is seen on hard surface compared to soft surface. More between-measurement variation is seen on the circle compared to the straight line. Between-horse variation was clearly larger than within-horse variation. ICC values for the head, withers and pelvis symmetry parameters were 0.68 (head), 0.76 (withers), 0.85 (pelvis). MAIN LIMITATIONS: Lunge measurements on a hard surface were not performed. CONCLUSIONS: Between-measurement variation may be substantial, especially in head motion. This should be considered when interpreting clinical data after repeated measurements, as in routine lameness assessments.

The development of hoof balance and landing preference in the post-natal period.

BACKGROUND: Foals can follow the herd within hours of birth, but it has been shown that kinetic gait parameters and static balance still have to mature. However, development of dynamic balance has not been investigated. OBJECTIVES: To objectively quantify landing and pressure pattern dynamics under the hoof during the first half year of life. STUDY DESIGN: Prospective, cohort study performed at a single stud farm. METHODS: Pressure plate measurements at walk and trot from ten Dutch warmblood foals during the first 24 weeks of life were used to quantify toe-heel and medial-lateral hoof balance asymmetry indexes and to determine preferred landing strategy. Concurrently, radiographs of the tarsocrural and femoropatellar joints were taken at 4-6 weeks and after 6 months to check for osteochondrosis. A linear mixed model was used to determine the effects of time point, limb pair (front/hind), side (left/right) and osteochondrosis status of every foal. RESULTS: At 25% of stance duration at walk, front limbs were more loaded in the heel region in weeks 6-20 (P≤0.04), the medial-lateral balance was more to the lateral side from week 6 onwards at both walk and trot (P≤0.04). Landing preference gradually changed in the same directions. Variability in pressure distribution decreased over time. (Subclinical) osteochondrosis did not influence any of the measured parameters. MAIN LIMITATIONS: This study is limited by the relatively small sample size only containing one breed from a single stud farm. CONCLUSIONS: Dynamic hoof balance in new-born foals is more variable and less oriented towards the lateral side of the hoof and to the heel than in mature horses. This pattern changes gradually during the first weeks of life. Knowledge of this process is essential for the clinician when considering interventions in this area in early life.

On the brink of daily clinical application of objective gait analysis: What evidence do we have so far from studies using an induced lameness model?

Quantitative gait analysis has the potential to offer objective and unbiased gait information that can assist clinical decision-making. In recent years, a growing number of gait analysis systems have come onto the market, highlighting the demand for such technology in equine orthopaedics. However, it is imperative that the measured variables which are used as outcome parameters are supported by scientific evidence and that the interpretation of such measurements is backed by a proper understanding of the biomechanical principles of equine locomotion. This review, which is based on studies on experimentally induced lameness, summarises the currently most widely used methods for gait analysis and the available evidence concerning gait parameters that can be used to quantify gait changes due to lameness. These are discussed regarding their current and future potential for routine clinical application.

Vertical movement symmetry of the withers in horses with induced forelimb and hindlimb lameness at trot.

BACKGROUND: The main criteria for lameness assessment in horses are head movement for forelimb lameness and pelvic movement for hindlimb lameness. However, compensatory head nod in horses with primary hindlimb lameness is a well-known phenomenon. This compensatory head nod movement can be easily misinterpreted as a sign of primary ipsilateral forelimb lameness. Therefore, discriminating compensatory asymmetries from primary directly pain-related movement asymmetries is a prerequisite for successful lameness assessment. OBJECTIVES: To investigate the association between head, withers and pelvis movement asymmetry in horses with induced forelimb and hindlimb lameness. STUDY DESIGN: Experimental study. METHODS: In 10 clinically sound Warmblood riding horses, forelimb and hindlimb lameness were induced using a sole pressure model. The horses were then trotted on a treadmill. Three-dimensional optical motion capture was used to collect kinematic data from reflective markers attached to the poll, withers and tubera sacrale. The magnitude and side (left or right) of the following symmetry parameters, vertical difference in minimum position, maximum position and range-up were calculated for head, withers, and pelvis. Mixed models were used to analyse data from induced forelimb and hindlimb lameness. RESULTS: For each mm increase in pelvic asymmetry in response to hindlimb lameness induction, withers movement asymmetry increased by 0.35-0.55 mm, but towards the contralateral side. In induced forelimb lameness, for each mm increase in head movement asymmetry, withers movement asymmetry increased by 0.05-0.10 mm, in agreement with the head movement asymmetry direction, both indicating lameness in the induced forelimb. MAIN LIMITATIONS: Results must be confirmed in clinically lame horses trotting overground. CONCLUSIONS: The vertical asymmetry pattern of the withers discriminated a head nod associated with true forelimb lameness from the compensatory head movement asymmetry caused by primary hindlimb lameness. Measuring movement symmetry of the withers may, thus, aid in determining primary lameness location.

Mouldable, thermoplastic, glue-on frog-supportive shoes change hoof kinetics in normal and obese Shetland ponies.

BACKGROUND: Obesity and hyperinsulinaemia are frequently encountered in the equine population and risk factors for the development of laminitis. There are many options for hoof support that claim a beneficial effect, but often the scientific evidence is scarce. OBJECTIVES: To quantify the effect of frog-supportive shoes on hoof kinetics in normal and obese ponies. STUDY DESIGN: Controlled in vivo trial. METHODS: Ten Shetland mares (n = 10) with a normal (n = 5) or obese (n = 5) body condition were led over a dynamically calibrated pressure plate before (T0), immediately after (T1) and 72 h (T2) after application of the shoes. The following locomotor variables were measured: stance duration (StDur), vertical impulse (VI), peak vertical force (PVF), time to PVF and time from PVF to lift off. The hoof print was divided into a toe and heel region and the StDur toe-heel index was calculated. The toe-heel hoof balance curves of the vertical force were plotted throughout the stance phase. RESULTS: The VI and PVF increased significantly 72 h after application of the shoes, when compared with T0 and T1. The StDur toe-heel index and toe-heel balance curves were significantly different between the normal and obese ponies. These variables became more comparable between the groups after application of the frog-supportive shoes. MAIN LIMITATIONS: It would have been interesting to measure the effect of the shoe in patients with acute laminitis. However, this would have had major welfare implications. CONCLUSIONS: The obese ponies moved more carefully than the normal group, demonstrated by a decreased loading of the toe area. The data illustrate that the ponies became more comfortable 72 h after application of the shoes, with a pronounced effect in the obese group. Thus, these results suggest that frog-supportive shoes could be beneficial, especially for obese ponies.

Quantification of the effect of instrumentation error in objective gait assessment in the horse on hindlimb symmetry parameters.

BACKGROUND: Objective gait analysis is becoming more popular as a tool assisting veterinarians during the clinical lameness exam. At present, there is only limited information on the effect of misplacement of markers/motion-sensors. OBJECTIVES: To investigate and describe the effect of marker misplacement on commonly calculated pelvic symmetry parameters. STUDY DESIGN: Experimental study. METHODS: Each horse was equipped with custom-made devices consisting of several reflective markers arranged in a predefined manner with a reference marker correctly positioned regarding the anatomical landmark and several misplaced markers along the sagittal and transverse planes. Linear regression analysis was used to estimate the effect of marker misplacement. RESULTS: For the tubera sacrale, each cm of left/right misplacement led to a difference in minimum position of the pelvis (PDmin) of ±1.67 mm (95% CI 1.54-1.8 mm) (P<0.001); maximum position of the pelvis (PDmax) was affected by ±0.2 mm (95% CI 0.071-0.33 mm) (P = 0.003). With respect to cranial/caudal misplacement, each cm of misplacement resulted in a PDmin difference of ±0.04 mm (95% CI -0.09 to 0.16 mm) (P = 0.56) and a PDmax difference of ±0.008 mm (95% CI -0.13 to 0.12 mm) (P = 0.9). For the tubera coxae, each cm of vertical misplacement led to a difference in the displacement amplitude between left and right tubera coxae (Hip-Hike_Diff) of ±1.56 mm (95% CI 1.35-1.77 mm) (P<0.001); for the cranial/caudal misplacement, this was ±0.82 mm (95% CI 0.66-0.97 mm) (P<0.001). MAIN LIMITATIONS: Only three horses were used in this experiment and the study design did not permit to determine the influence of marker misplacement on the evaluation of different degrees of lameness. CONCLUSIONS: Marker misplacement significantly affects calculated symmetry parameters of the pelvis. The observed errors are overall small but significant. In cases of mildly asymmetrical horses, this error might influence the decision-making process whereas in more severe asymmetries, the influence of the error effect may become less significant.

The development of locomotor kinetics in the foal and the effect of osteochondrosis.

REASON FOR PERFORMING STUDY: Foals stand and walk immediately after birth, but insight into the subsequent longitudinal development of their gait kinetics in the early juvenile phase and the possible influence of osteochondrosis thereon is lacking. OBJECTIVES: To quantify gait kinetics in foals during the first half year of life, taking into account their osteochondrosis status. STUDY DESIGN: Prospective, cohort study performed at a single stud farm. METHODS: Pressure plate measurements at walk and trot from 11 Dutch Warmblood foals during the first 24 weeks of life were used to determine body mass normalised peak vertical force, normalised vertical impulse and stance duration. Coefficients of variation of peak vertical force and stance duration were used as measures for gait maturity. Radiographs of tarsocrural and femoropatellar joints were taken at age 4-6 weeks and after 6 months to check for osteochondrosis. A linear mixed model was used to determine the effects of age, limb, presence of osteochondrosis and speed on gait parameters. RESULTS: Mean walking and trotting velocity increased over time as did stance duration and normalised vertical impulse, normalised peak vertical force values however remained relatively constant. During the first weeks of their life only the coefficient of variation of stance duration decreased significantly, while the coefficient of variation of peak vertical force did not. None of the foals was visibly lame, but the presence of osteochondrosis resulted in a temporarily but significantly reduced normalised peak vertical force. MAIN LIMITATIONS: This study is a relatively small sample size of one breed from a single stud farm. A stand-alone pressure plate was used and body mass was estimated rather than measured. CONCLUSIONS: Despite being precocious, foals need time to mature their gait. During growth, velocity at walk and trot increases, but normalised peak vertical force remains relatively constant. Although not visibly lame, a temporary reduction in normalised peak vertical force was detected in osteochondrosis positive foals using a pressure plate.