Evaluation of the effects of chitosan nanoparticles on polyhydroxy butyrate electrospun scaffolds for cartilage tissue engineering applications.

In this study, we synthesized and incorporated chitosan nanoparticles (Cs) into polyhydroxy butyrate (PHB) electrospun scaffolds for cartilage tissue engineering. The Cs nanoparticles were synthesized via an ionic gel interaction between Cs powder and tripolyphosphate (TPP). The mechanical properties, hydrophilicity, and fiber diameter of the PHB scaffolds with varying concentrations of Cs nanoparticles (1-5 wt%) were evaluated. The results of these evaluations showed that the scaffold containing 1 wt% Cs nanoparticles (P1Cs) was the optimum scaffold, with increased ultimate strength from 2.6 to 5.2 MPa and elongation at break from 5.31 % to 12.6 %. Crystallinity, degradation, and cell compatibility were also evaluated. The addition of Cs nanoparticles decreased crystallinity and accelerated hydrolytic degradation. MTT assay results showed that the proliferation of chondrocytes on the scaffold containing 1 wt% Cs nanoparticles were significantly higher than that on pure PHB after 7 days of cultivation. These findings suggest that the electrospun P1Cs scaffold has promising potential as a substrate for cartilage tissue engineering applications. This combination offers a promising approach for the fabrication of biomimetic scaffolds with enhanced mechanical properties, hydrophilicity, and cell compatibility for tissue engineering applications.

Ecotoxicity of chlorpyrifos on earthworm Eisenia fetida (Savigny, 1826): Modifications in oxidative biomarkers.

Chlorpyrifos (CPF; O, O'-diethyl-3, 5, 6-trichloro-2-pyridyl phosphorothionate) as an organophosphate compound with moderate toxicity that entered the soil in 1965, is widely used as an active substance of many insecticides. CPF may affect some biochemical mechanisms, particularly through disrupting pro- and anti-oxidant balance and inducing free radical-induced oxidative stress. Expired pesticides, if present in ecosystem, may pose new issues of toxicological concern. In the current study, modifications in the oxidative stress (OS) hallmarks including the content of lipid peroxidation (LPO/MDA) and the activities of antioxidant enzymes catalase (CAT) and glutathione S-transferase (GST) in the whole body extract and total antioxidant capacity (TAC), in the coelomic fluid (CF) of earthworm Eisenia fetida were evaluated spectrophotometrically after exposure to different concentrations (1/20th, 1/10th, and 1/5th of LC50) of fresh and expired CPF for 4 and 8 days. First, LC50 for both fresh and expired CPF were determined by using probit method as ≤192 and ≤ 209 mg/kg dry soil, respectively. Our results also revealed that both fresh and expired CPF could be toxic to earthworms via inducing OS at higher concentrations. Here, CPF-induced OS was determined by a significant elevation (p < 0.05) in LPO content, CAT and GST activities and also a meaningful decrease (p < 0.05) in TAC value. Briefly, CPF may exhibit toxic effects in earthworms in the fresh and expired forms via changing oxidative balance and modifying some biochemical markers in the whole body. Further unraveling is needed to elucidate CPF-related impairments in soil organisms.