Comprehensive Analysis of Contaminants in Powdered Milk Samples Using an HPGe for γ Radiation.

Aims: This study aimed to assess the activity concentrations and cancer risk assessments of 232Th and 40K in powdered milk samples collected from various suppliers in Pakistan, considering the increasing concern about cancer risks associated with environmental radiological effects related to food consumption. Subjects and Methods: Specific activity concentrations were determined using a high-resolution, high-purity germanium γ-spectroscopy system. Results: The specific activity levels of 40K and 232Th in the analyzed powdered milk samples were found to be 230.86 and 6.87 Bq/kg, respectively, well within the safe limits recommended by the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR). The hazard index (0.074 Bq/kg) and radium equivalent (27.58 Bq/kg) were calculated as indicators of radiation hazard, along with absorbed dose (26.26 nGy/h), annual effective dose (0.13 nGy/h), and excess lifetime cancer risk (0.45). These parameters provide insights into the potential health risks associated with powdered milk consumption. Conclusions: The findings collectively affirm the radiological safety of the analyzed powdered milk samples, providing valuable insights into the potential health risks associated with their consumption.

Study loss of vegetative cover and increased land surface temperature through remote sensing strategies under the inter-annual climate variability in Jinhua-Quzhou basin, China.

The Jinhua-Quzhou basin in China is one of the most susceptible areas to drought. Due to the loss of vegetation and great fluctuations in rainfall and surface temperature, global warming occurs. Timely, accurate, and effective drought monitoring is crucial for protecting local vegetation and determining which vegetation is most vulnerable to increased LST during the period 1982-2019. It assumes a strong correlation between loss of vegetation cover, changes in monsoon climate, drought, and increases in land surface temperature (LST). Due to significantly increased in LST, low precipitation and vegetation cover, NDVI, TVDI, VCI, and NAP are useful in characterizing drought mitigation strategies. The temperature vegetation drought index (TVDI), normalized difference vegetation index (NDVI), vegetation condition index (VCI), and monthly precipitation anomaly percentage (NAP) can be helped to characterize drought reduction strategies. Monthly NDVI, NAP, VCI, TVDI, normalized vegetation supply water index (NVSWI), temperature condition index (TCI), vegetation health index (VHI), and heat map analysis indicate that the Jinhua-Quzhou basin experienced drought during 1984, 1993, 2000, and 2011. Seasonal SR, WVP, WS, NDVI, VCI, and NAP charts confirm that the Jinhua-Quzhou basin was affected by severe drought in 1984, which continued and led to severe droughts in 1993, 2000, and 2011. Regression analysis showed a significant positive correlation between NDVI, TVDI, VCI, and NAP values, while NVSWI, TVDI, and VHI showed positive signs of good drought monitoring strategies. The research results confirm the correlation between loss of vegetation cover and LST, which is one of the causes of global warming. The distribution of drought changed a trend indicating that compared with the Jinhua region; the Quzhou region has more droughts. The changing trend of drought has characteristics from 1982 to 2019, and there are significant differences in drought changing trends between different Jinhua-Quzhou basin areas. Overall, from 1982 to 2019, the frequency of drought showed a downward trend. We believe that these results will provide useful tools for drought management plans and play a relevant role in mitigating the effects of drought and protecting humanity from climate hazards.

Regulation of hippo signaling mediated apoptosis by Rauvolfia tetraphylla in triple-negative breast cancer.

Rauvolfia tetraphylla is an essential medicinal plant that has been widely used in traditional medicine for various disease treatments. However, the tumor suppressor activity of R. tetraphylla and its phytocompounds were not explored against triple-negative breast cancer. The current research investigated the impact of R. tetraphylla methanolic extract (RTE) and its isolated compounds Ajmaline (RTC1) and Reserpine (RTC2) on triple-negative breast cancer cell line (MDA-MB-231) focusing on anti-proliferative effects. Our study imparts that RTE and RTC2 showed promising cytotoxic effects compared to RTC1. So further experiments have proceeded with RTE and RTC2, to evaluate its proliferation, migration, and apoptotic effect. The result shows around 80% of cells were observed in the G0/G1 phase in cell cycle analysis indicating the cell cycle inhibition and duel staining clearly showed the apoptotic effect. The migration of cells after the scratch was 60.45% observed in control and 90% in treated cells showing the inhibition of migration. ROS distribution was intense compared to control indicating the increased ROS stress in treated cells. Both RTE and RTC2-treated cells showed the potential to suppress proliferation and induce apoptotic change by upregulating BAX and MST-1 and suppressing Bcl2, LATS-1, and YAP, proving that deregulation of YAP resulting in the blockage of TEAD-YAP complex and inhibit proliferation. Therefore, R. tetraphylla extract and its isolated compounds were demonstrated to find its ability to act against MDA-MB-231 and these findings will help adjudicate it as a therapeutic drug against experimental triple-negative breast cancer.

The Role of Climate Change and Its Sensitivity on Long-Term Standardized Precipitation Evapotranspiration Index, Vegetation and Drought Changing Trends over East Asia.

Droughts have become more severe and frequent due to global warming. In this context, it is widely accepted that for drought assessments, both water supply (rainfall) and demand (standardized precipitation evapotranspiration index, SPEI) should be considered. Using SPEI, we explored the spatial-temporal patterns of dry and wet annual and seasonal changes in five sub-regions of East Asia during 1902-2018. These factors are linked to excess drought frequency and severity on the regional scale, and their effect on vegetation remains an important topic for climate change studies. Our results show that the SPEI significantly improved extreme drought and mostly affected the SPEI-06 and SPEI-12 growing seasons in East Asia during 1981-2018. The dry and wet annual SPEI trends mostly affect the five sub-regions of East Asia. The annual SPEI had two extremely dry spells during 1936-1947 and 1978-2018. Japan, South Korea, and North Korea are wet in the summer compared to other regions of East Asia, with drought frequency occurring at 51.4%, respectively. The mean drought frequencies in China and Mongolia are 57.4% and 54.6%. China and Mongolia are the driest regions in East Asia due to high drought frequency and duration. The spatial seasonal analysis of solar radiation (SR), water vapor pressure (WVP), wind speed (WS), vegetation condition index (VCI), temperature condition index (TCI), and vegetation health index (VHI) have confirmed that the East Asia region suffered from maximum drought events. The seasonal variation of SPEI shows no clear drying trends during summer and autumn seasons. During the winter and spring seasons, there was a dry trend in East Asia region. During 1902-1990, a seasonal SPEI presented diverse characteristics, with clear wet trends in Japan, Mongolia, and North Korea in four different growing seasons, with dry trends in China and South Korea. During 1991-2018, seasonal SPEI presented clear dry trends in Japan, Mongolia, and North Korea in different growing seasons, while China and South Korea showed a wet trend during the spring, autumn, and winter seasons. This ecological and climatic mechanism provides a good basis for the assessment of vegetation and drought-change variations within East Asia. An understandings of long-term vegetation trends and the effects of rainfall and SPEI on droughts of varying severity is essential for water resource management and climate change adaptation. Based on the results, water resources will increase under global warming, which may alleviate the water scarcity issue in the East Asia region.

Zinc oxide nanoparticles functionalized with cinnamic acid for targeting dental pathogens receptor and modulating apoptotic genes in human oral epidermal carcinoma KB cells.

BACKGROUND: Oral diseases are often attributed to dental pathogens such as S. aureus, S. mutans, E. faecalis, and C. albicans. In this research work, a novel approach was employed to combat these pathogens by preparing zinc oxide nanoparticles (ZnO NPs) capped with cinnamic acid (CA) plant compounds. METHODS: The synthesized ZnO-CA NPs were characterized using SEM, FTIR, and XRD to validate their composition and structural features. The antioxidant activity of ZnO-CA NPs was confirmed using DPPH and ABTS free radical scavenging assays. The antimicrobial effects of ZnO-CA NPs were validated using a zone of inhibition assay against dental pathogens. Autodock tool was used to identify the interaction of cinnamic acid with dental pathogen receptors. RESULTS: ZnO-CA NPs exhibited potent antioxidant activity in both DPPH and ABTS assays, suggesting their potential as powerful antioxidants. The minimal inhibitory concentration of ZnO-CA NPs against dental pathogens was found 25 µg/mL, indicating their effective antimicrobial properties. Further, ZnO-CA NPs showed better binding affinity and amino acid interaction with dental pathogen receptors. Also, the ZnO-CA NPs exhibited dose-dependent (5 µg/mL, 15 µg/mL, 25 µg/mL, and 50 µg/mL) anticancer activity against Human Oral Epidermal Carcinoma KB cells. The mechanism of action of apoptotic activity of ZnO-CA NPs on the KB cells was identified through the upregulation of BCL-2, BAX, and P53 genes. CONCLUSIONS: This research establishes the potential utility of ZnO-CA NPs as a promising candidate for dental applications. The potent antioxidant, anticancer, and effective antimicrobial properties of ZnO-CA NPs make them a valuable option for combating dental pathogens.

Green, Solvent-Free Mechanochemical Synthesis of Nano Ag2O/MnO2/N-Doped Graphene Nanocomposites: An Efficient Catalyst for Additive-Base-Free Aerial Oxidation of Various Kinds of Alcohols.

Herein, we report a solvent-less, straightforward, and facile mechanochemical technique to synthesize nanocomposites of Ag2O nanoparticles-doped MnO2, which is further codoped with nitrogen-doped graphene (N-DG) [i.e., (X %)N-DG/MnO2-(1% Ag2O)] using physical milling of separately prepared N-DG and Ag2O NPs-MnO2 annealed at 400 °C over an eco-friendly ball-mill process. To assess the efficiency in terms of catalytic performance of the nanocomposites, selective oxidation of benzyl alcohol (BlOH) to benzaldehyde (BlCHO) is selected as a substrate model with an eco-friendly oxidizing agent (O2 molecule) and without any requirements for the addition of any harmful additives or bases. Various nanocomposites were prepared by varying the amount of N-DG in the composite, and the results obtained highlighted the function of N-DG in the catalyst system when they are compared with the catalyst MnO2-(1% Ag2O) [i.e., undoped catalyst] and MnO2-(1% Ag2O) codoped with different graphene dopants such as GRO and H-RG for alcohol oxidation transformation. The effects of various catalytic factors are systematically evaluated to optimize reaction conditions. The N-DG/MnO2-(1% Ag2O) catalyst exhibits premium specific activity (16.0 mmol/h/g) with 100% BlOH conversion and <99.9% BlCHO selectivity within a very short interval. The mechanochemically prepared N-DG-based nanocomposite displayed higher catalytic efficacy than that of the MnO2-(1% Ag2O) catalyst without the graphene dopant, which is N-DG in this study. A wide array of aromatic, heterocyclic, allylic, primary, secondary, and aliphatic alcohols have been selectively converted to respective ketones and aldehydes with full convertibility without further oxidation to acids over N-DG/MnO2-(1% Ag2O). Interestingly, it is also found that the N-DG/MnO2-(1% Ag2O) can be efficiently reused up to six times without a noteworthy decline in its effectiveness. The prepared nanocomposites were characterized using various analytical, microscopic, and spectroscopic techniques such as X-ray diffraction, thermogravimetric analysis, Fourier-transform infrared spectroscopy, Raman, field emission scanning electron microscopy, and Brunauer-Emmett-Teller.

Solvothermal synthesis of VO2 nanoparticles with locally patched V2O5 surface layer and their morphology-dependent catalytic properties for the oxidation of alcohols.

Vanadium oxides are promising oxidation catalysts because of their rich redox chemistry. We report the synthesis of VO2 nanocrystals with VO2(B) crystal structure. By varying the mixing ratio of the components of a binary ethanol/water mixture, different VO2 nanocrystal morphologies (nanorods, -urchins, and -sheets) could be made selectively in pure form. Polydisperse VO2(B) nanorods with lengths between 150 nm and a few micrometers were formed at large water : ethanol ratios between 4 : 1 and 3 : 2. At a water : ethanol ratio of 1 : 9 VO2 nanosheets with diameters of ∼50-70 nm were formed, which aggregated to nano-urchins with diameters of ∼200 nm in pure ethanol. The catalytic activity of VO2 nanocrystals for the oxidation of alcohols was studied as a function of nanocrystal morphology. VO2 nanocrystals with all morphologies were catalytically active. The activity for the oxidation of benzyl alcohol to benzaldehyde was about 30% higher than that for the oxidation of furfuryl alcohol to furfural. This is due to the substrate structure. The oxidation activity of VO2 nanostructures decreases in the order of nanourchins > nanosheets > nanorods.

Marine-derived κ-carrageenan-coated zinc oxide nanoparticles for targeted drug delivery and apoptosis induction in oral cancer.

BACKGROUND: Kappaphycus alvarezii, a marine red algae species, has gained significant attention in recent years due to its versatile bioactive compounds. Among these, κ-carrageenan (CR), a sulfated polysaccharide, exhibits remarkable antimicrobial properties. This study emphasizes the synergism attained by functionalizing zinc oxide nanoparticles (ZnO NPs) with CR, thereby enhancing its antimicrobial efficacy and target specificity against dental pathogens. METHODS: In this study, we synthesized ZnO-CR NPs and characterized them using SEM, FTIR, and XRD techniques to authenticate their composition and structural attributes. Moreover, our investigation revealed that ZnO-CR NPs possess better free radical scavenging capabilities, as evidenced by their effective activity in the DPPH and ABTS assay. RESULTS: The antimicrobial properties of ZnO-CR NPs were systematically assessed using a zone of inhibition assay against dental pathogens of S. aureus, S. mutans, E. faecalis, and C. albicans, demonstrating their substantial inhibitory effects at a minimal concentration of 50 µg/mL. We elucidated the interaction between CR and the receptors of dental pathogens to further understand their mechanism of action. The ZnO-CR NPs demonstrated a dose-dependent anticancer effect at concentrations of 5 µg/mL, 25 µg/mL, 50 µg/mL, and 100 µg/mL on KB cells, a type of Human Oral Epidermal Carcinoma. The mechanism by which ZnO-CA NPs induced apoptosis in KB cells was determined by observing an increase in the expression of the BCL-2, BAX, and P53 genes. CONCLUSION: Our findings unveil the promising potential of ZnO-CR NPs as a candidate with significant utility in dental applications. The demonstrated biocompatibility, potent antioxidant and antiapoptotic activity, along with impressive antimicrobial efficacy position these NPs as a valuable resource in the ongoing fight against dental pathogens and oral cancer.

Sb-Doped Cerium Molybdate: An Emerging Material as Dielectric and Photocatalyst for the Removal of Diclofenac Potassium from Aqueous Media.

This work reports the influence of antimony substitution in a cerium molybdate lattice for improved dielectric and photocatalytic properties. For this purpose, a series of Ce2-xSbx(MoO4)3 (x = 0.00, 0.01, 0.03, 0.05, 0.07, and 0.09) were synthesized through a co-precipitation route. The as-synthesized materials were characterized for their optical properties, functional groups, chemical oxidation states, structural phases, surface properties, and dielectric characteristics using UV-Vis spectroscopy (UV-Vis), Fourier transform infrared (FTIR) and Raman spectroscopies, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, and impedance spectroscopy, respectively. UV-Vis study showed a prominent red shift of absorption maxima and a continuous decrease in band gap (3.35 eV to 2.79 eV) by increasing the dopant concentration. The presence of Ce-O and Mo-O-Mo bonds, detected via FTIR and Raman spectroscopies, are confirmed, indicating the successful synthesis of the desired material. The monoclinic phase was dominant in all materials, and the crystallite size was decreased from 40.29 nm to 29.09 nm by increasing the Sb content. A significant increase in the dielectric constant (ε' = 2.856 × 108, 20 Hz) and a decrease in the loss tan (tanδ = 1.647, 20 Hz) were exhibited as functions of the increasing Sb concentration. Furthermore, the photocatalytic efficiency of pristine cerium molybdate was also increased by 1.24 times against diclofenac potassium by incorporating Sb (x = 0.09) in the cerium molybdate. The photocatalytic efficiency of 85.8% was achieved within 180 min of UV light exposure at optimized conditions. The photocatalytic reaction followed pseudo-first-order kinetics with an apparent rate constant of 0.0105 min-1, and the photocatalyst was recyclable with good photocatalytic activity even after five successive runs. Overall, the as-synthesized Sb-doped cerium molybdate material has proven to be a promising candidate for charge storage devices and a sustainable photocatalyst for wastewater treatment.

Synthesis and Structural Characterization of Selenium Nanoparticles-Bacillus sp. MKUST-01 Exopolysaccharide (SeNPs-EPS) Conjugate for Biomedical Applications.

Exopolysaccharides (EPS) are exogenous microbial metabolites generated predominantly during the development of bacteria. They have several biological potentials, including antibacterial, antioxidant, and anticancer actions. Polysaccharide-coated nanoparticles have high biological activity and are used in treatments and diagnostics. In this research, selenium nanoparticles (SeNPs) are synthesized and conjugated with bacterial (Bacillus sp. MKUST-01) exopolysaccharide (EPS). Initially, the creation of SeNPs conjugates was verified through UV-Vis spectral examination, which exhibited a prominent peak at 264 nm. Additionally, X-ray diffraction (XRD) analysis further substantiated the existence of crystalline Se, as evidenced by a robust reflection at 29.78°. Another reflection observed at 23.76° indicated the presence of carbon originating from the EPS. Fourier transform infrared spectroscopy (FT-IR) analysis of the EPS capped with SeNPs displayed characteristic peaks at 3425 cm-1, 2926 cm-1, 1639 cm-1, and 1411 cm-1, corresponding to the presence of O-H, C-H, C=O, and COO-groups. The SeNPs themselves were found to possess elongated rod-shaped structures with lengths ranging from 250 to 550 nm and a diameter of less than 70 nm, as confirmed using scanning electron microscopy and particle size analysis. In contrast to the SeNPs, the SeNPs-EPS conjugates showed no hemolytic activity. The overall antioxidant activity of SeNPs-EPS conjugates outperformed 20% higher than SeNPs and EPS. Additionally, experimental observations involving gnotobiotic Artemia nauplii experiments were also recorded, such as the supplementation of EPS and SeNPs-EPS conjugates corresponding to enhanced growth and increased survival rates compared to Artemia nauplii fed with SeNPs and a microalgal diet.

Morphology Controlled Deposition of Vanadium Oxide (VOx) Nanoparticles on the Surface of Highly Reduced Graphene Oxide for the Photocatalytic Degradation of Hazardous Organic Dyes.

Semiconducting nanomaterials based heterogeneous photocatalysis represent a low-cost, versatile technique for environmental remediation, including pollution mitigation, energy management and other environmental aspects. Herein, we demonstrate the syntheses of various heterogeneous photocatalysts based on highly reduced graphene oxide (HRG) and vanadium oxide (VOx)-based nanocomposites (HRG-VOx). Different shapes (rod, sheet and urchin forms) of VOx nanoparticles were successfully fabricated on the surface of HRG under solvo-/hydrothermal conditions by varying the amount of water and ethanol. The high concentration of water in the mixture resulted in the formation of rod-shaped VOx nanoparticles, whereas increasing the amount of ethanol led to the production of VOx sheets. The solvothermal condition using pure ethanol as solvent produced VOx nano-urchins on the surface of HRG. The as-prepared hybrid materials were characterized using various spectroscopic and microscopic techniques, including X-ray diffraction, UV-vis, FTIR, SEM and TEM analyses. The photocatalytic activities of different HRG-VOx nanocomposites were investigated for the photodegradation of methylene blue (MB) and methyl orange (MO). The experimental data revealed that all HRG-VOx composite-based photocatalysts demonstrated excellent performance toward the photocatalytic degradation of the organic dyes. Among all photocatalysts studied, the HRG-VOx nanocomposite consisting of urchin-shaped VOx nanoparticles (HRG-VOx-U) demonstrated superior photocatalytic properties towards the degradation of dyes.

CBPDdb: a curated database of compounds derived from Coumarin-Benzothiazole-Pyrazole.

The present article describes the building of a small-molecule web server, CBPDdb, employing R-shiny. For the generation of the web server, three compounds were chosen, namely coumarin, benzothiazole and pyrazole, and their derivatives were curated from the literature. The two-dimensional (2D) structures were drawn using ChemDraw, and the .sdf file was created employing Discovery Studio Visualizer v2017. These compounds were read on the R-shiny app using ChemmineR, and the dataframe consisting of a total of 1146 compounds was generated and manipulated employing the dplyr package. The web server is provided with JSME 2D sketcher. The descriptors of the compounds are obtained using propOB with a filter. The users can download the filtered data in the .csv and .sdf formats, and the entire dataset of a compound can be downloaded in .sdf format. This web server facilitates the researchers to screen plausible inhibitors for different diseases. Additionally, the method used in building the web server can be adapted for developing other small-molecule databases (web servers) in RStudio. Database URL: https://srampogu.shinyapps.io/CBPDdb_Revised/.

Development and Characterization of Bio-Based Composite Films for Food Packing Applications Using Boiled Rice Water and Pistacia vera Shells.

Customer demand for natural packaging materials in the food industry has increased. Biocomposite films developed using boiled rice water could be an eco-friendly and cost-effective packaging product in the future. This study reports the development of bio-based films using waste materials, such as boiled rice water (matrix) and Pistacia vera shells (reinforcement material), using an adapted solution casting method. Several film combinations were developed using various concentrations of plasticizing agent (sorbitol), thickening agent (oil and agar), and stabilizing agents (Arabic gum, corn starch, and Pistacia vera shell powder). Various packaging properties of the film were analyzed and examined to select the best bio-based film for food packaging applications. The film fabricated with Pistacia vera shell powder in the biocomposite film exhibited a reduced water solubility, swelling index, and moisture content, as compared to polyethene packaging material, whereas the biocomposite film exhibited poor antimicrobial properties, high vapor transmission rate, and high biodegradability rate. The packaging properties and characterization of the film indicated that the boiled rice water film with Pistacia vera shell powder was suitable for packaging material applications.

Evaluation of Photocatalytic, Antioxidant, and Antibacterial Efficacy of Almond Oil Capped Zinc Oxide Nanoparticles.

In this study, ZnO nanoparticles (NPs) were synthesized in the presence of almond oil at various molar ratios of zinc acetate and sodium hydroxide, including 0.5:1, 0.75:1, 1:1, 1.25:1, and 1.5:1, to obtain pH values of 11, 10, 9, 8, and 7, respectively. The XRD results revealed that ZnO NPs exhibit a hexagonal structure, with high crystallinity. SEM results showed that dense and large sized ZnO NPs were formed at pH 11, and relatively small (~30-40 nm) NPs were obtained at pH 9. The size distribution can be explained in terms of the presence of OH- ions at different pH levels. However, the larger size of the NPs at pH 7 compared to those at pH 8-11 were due to the coalescence of NPs suitable for antioxidant/antibacterial activities. ZnO NPs demonstrated a high degradation efficiency (~93%) in 90 min, with a high rate constant for Methyl Orange (MO), which is better than the previously reported rate. The larger sized almond oil capped ZnO NPs also showed excellent radical scavenging activity (94%) and are proven to be good carriers to resist Escherichia coli (E. coli) bacteria.

OralNet: Fused Optimal Deep Features Framework for Oral Squamous Cell Carcinoma Detection.

Humankind is witnessing a gradual increase in cancer incidence, emphasizing the importance of early diagnosis and treatment, and follow-up clinical protocols. Oral or mouth cancer, categorized under head and neck cancers, requires effective screening for timely detection. This study proposes a framework, OralNet, for oral cancer detection using histopathology images. The research encompasses four stages: (i) Image collection and preprocessing, gathering and preparing histopathology images for analysis; (ii) feature extraction using deep and handcrafted scheme, extracting relevant features from images using deep learning techniques and traditional methods; (iii) feature reduction artificial hummingbird algorithm (AHA) and concatenation: Reducing feature dimensionality using AHA and concatenating them serially and (iv) binary classification and performance validation with three-fold cross-validation: Classifying images as healthy or oral squamous cell carcinoma and evaluating the framework's performance using three-fold cross-validation. The current study examined whole slide biopsy images at 100× and 400× magnifications. To establish OralNet's validity, 3000 cropped and resized images were reviewed, comprising 1500 healthy and 1500 oral squamous cell carcinoma images. Experimental results using OralNet achieved an oral cancer detection accuracy exceeding 99.5%. These findings confirm the clinical significance of the proposed technique in detecting oral cancer presence in histology slides.

Effectiveness of Nonfunctionalized Graphene Oxide Nanolayers as Nanomedicine against Colon, Cervical, and Breast Cancer Cells.

Recent studies in nanomedicine have intensively explored the prospective applications of surface-tailored graphene oxide (GO) as anticancer entity. However, the efficacy of nonfunctionalized graphene oxide nanolayers (GRO-NLs) as an anticancer agent is less explored. In this study, we report the synthesis of GRO-NLs and their in vitro anticancer potential in breast (MCF-7), colon (HT-29), and cervical (HeLa) cancer cells. GRO-NLs-treated HT-29, HeLa, and MCF-7 cells showed cytotoxicity in the MTT and NRU assays via defects in mitochondrial functions and lysosomal activity. HT-29, HeLa, and MCF-7 cells treated with GRO-NLs exhibited substantial elevations in ROS, disturbances of the mitochondrial membrane potential, an influx of Ca2+, and apoptosis. The qPCR quantification showed the upregulation of caspase 3, caspase 9, bax, and SOD1 genes in GRO-NLs-treated cells. Western blotting showed the depletion of P21, P53, and CDC25C proteins in the above cancer cell lines after GRO-NLs treatment, indicating its function as a mutagen to induce mutation in the P53 gene, thereby affecting P53 protein and downstream effectors P21 and CDC25C. In addition, there may be a mechanism other than P53 mutation that controls P53 dysfunction. We conclude that nonfunctionalized GRO-NLs exhibit prospective biomedical application as a putative anticancer entity against colon, cervical, and breast cancers.

Light-Driven Catalytic Activity of Green-Synthesized SnO2/WO3-x Hetero-nanostructures.

This work reports an environmentally friendly and economically feasible green synthesis of monometallic oxides (SnO2 and WO3) and their corresponding mixed metal oxide (SnO2/WO3-x) nanostructures from the aqueous Psidium guajava leaf extract for light-driven catalytic degradation of a major industrial contaminant, methylene blue (MB). P. guajava is a rich source of polyphenols that acts as a bio-reductant as well as a capping agent in the synthesis of nanostructures. The chemical composition and redox behavior of the green extract were investigated by liquid chromatography-mass spectrometry and cyclic voltammetry, respectively. Results acquired by X-ray diffraction and Fourier transform infrared spectroscopy confirm the successful formation of crystalline monometallic oxides (SnO2 and WO3) and bimetallic SnO2/WO3-x hetero-nanostructures capped with polyphenols. The structural and morphological aspects of the synthesized nanostructures were analyzed by transmission electron microscopy and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. Photocatalytic activity of the synthesized monometallic and hetero-nanostructures was investigated for the degradation of MB dye under UV light irradiation. Results indicate a higher photocatalytic degradation efficiency for mixed metal oxide nanostructures (93.5%) as compared to pristine monometallic oxides SnO2 (35.7%) and WO3 (74.5%). The hetero-metal oxide nanostructures prove to be better photocatalysts with reusability up to 3 cycles without any loss in degradation efficiency or stability. The enhanced photocatalytic efficiency is attributed to a synergistic effect in the hetero-nanostructures, efficient charge transportation, extended light absorption, and increased adsorption of dye due to the enlarged specific surface area.

Antimicrobial, anticancer, and biofilm inhibition studies of highly reduced graphene oxide (HRG): In vitro and in silico analysis.

Background: Bacterial infections and cancers may cause various acute or chronic diseases, which have become serious global health issues. This requires suitable alternatives involving novel and efficient materials to replace ineffective existing therapies. In this regard, graphene composites are being continuously explored for a variety of purposes, including biomedical applications, due to their remarkable properties. Methods: Herein, we explore, in-vitro, the different biological properties of highly reduced graphene oxide (HRG), including anti-cancer, anti-bacterial, and anti-biofilm properties. Furthermore, to analyze the interactions of graphene with proteins of microbes, in silico docking analysis was also carried out. To do this, HRG was prepared using graphene oxide as a precursor, which was further chemically reduced to obtain the final product. The as-prepared HRG was characterized using different types of microscopic and spectroscopic techniques. Results: The HRG revealed significant cytotoxic ability, using a dose-dependent anti-cell proliferation approach, which substantially killed human breast cancer cells (MCF-7) with IC50 of 29.51 ± 2.68 µg/mL. The HRG demonstrated efficient biological properties, i.e., even at low concentrations, HRG exhibited efficient anti-microbial properties against a variety of microorganisms. Among the different strains, Gram-positive bacteria, such as B. subtilis, MRSA, and S. aureus are more sensitive to HRG compared to Gram-negative bacteria. The bactericidal properties of HRG are almost similar to a commercially available effective antibiotic (ampicillin). To evaluate the efficacy of HRG against bacterial biofilms, Pseudomonas aeruginosa and MRSA were applied, and the results were compared with gentamycin and ampicillin, which are commonly applied standard antibiotics. Notably, HRG demonstrated high inhibition (94.23%) against P.aeruginosa, with lower MIC (50 µg/mL) and IC50 (26.53 µg/mL) values, whereas ampicillin and gentamicin showed similar inhibition (90.45% and 91.31% respectively) but much higher MIC and IC50 values. Conclusion: Therefore, these results reveal the excellent biopotential of HRG in different biomedical applications, including cancer therapy; antimicrobial activity, especially anti-biofilm activity; and other biomedicine-based therapies. Based on the molecular docking results of Binding energy, it is predicted that pelB protein and HRG would form the best stable docking complex, and high hydrogen and hydrophobic interactions between the pelB protein and HRG have been revealed. Therefore, we conclude that HRG could be used as an antibiofilm agent against P. aeruginosa infections.

Diversity of Citrullus colocynthis (L.) Schrad Seeds Extracts: Detailed Chemical Profiling and Evaluation of Their Medicinal Properties.

Seeds and fruits of Citrullus colocynthis have been reported to possess huge potential for the development of phytopharmaceuticals with a wide range of biological activities. Thus, in the current study, we are reporting the potential antimicrobial and anticancer properties of C. colocynthis seeds extracted with solvents of different polarities, including methanol (M.E.), hexane (H.E.), and chloroform (C.E.). Antimicrobial properties of C. colocynthis seeds extracts were evaluated on Gram-positive and Gram-negative bacteria, whereas, anticancer properties were tested on four different cell lines, including HepG2, DU145, Hela, and A549. All the extracts have demonstrated noteworthy antimicrobial activities with a minimum inhibitory concentration (MIC) ranging from 0.9-62.5 µg/mL against Klebsiella planticola and Staphylococcus aureus; meanwhile, they were found to be moderately active (MIC 62.5-250 µg/mL) against Escherichia coli and Micrococcus luteus strains. Hexane extracts have demonstrated the highest antimicrobial activity against K. planticola with an MIC value of 0.9 µg/mL, equivalent to that of the standard drug ciprofloxacin used as positive control in this study. For anticancer activity, all the extracts of C. colocynthis seeds were found to be active against all the tested cell lines (IC50 48.49-197.96 µg/mL) except for the chloroform extracts, which were found to be inactive against the HepG2 cell line. The hexane extract was found to possess the most prominent anticancer activity when compared to other extracts and has demonstrated the highest anticancer activity against the DU145 cell line with an IC50 value of 48.49 µg/mL. Furthermore, a detailed phytoconstituents analysis of all the extracts of C. colocynthis seeds were performed using GC-MS and GC-FID techniques. Altogether, 43 phytoconstituents were identified from the extracts of C. colocynthis seeds, among which 21, 12, and 16 components were identified from the H.E., C.E., and M.E. extracts, respectively. Monoterpenes (40.4%) and oxygenated monoterpenes (41.1%) were the most dominating chemical class of compounds from the hexane and chloroform extracts, respectively; whereas, in the methanolic extract, oxygenated aliphatic hydrocarbons (77.2%) were found to be the most dominating chemical class of compounds. To the best of our knowledge, all the phytoconstituents identified in this study are being reported for the first time from the C. colocynthis.

Self-Healing, Flexible and Smart 3D Hydrogel Electrolytes Based on Alginate/PEDOT:PSS for Supercapacitor Applications.

Hydrogel electrolytes for energy storage devices have made great progress, yet they present a major challenge in the assembly of flexible supercapacitors with high ionic conductivity and self-healing properties. Herein, a smart self-healing hydrogel electrolyte based on alginate/poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (alginate/PEDOT:PSS)(A/P:P) was prepared, wherein H2SO4 was employed as a polymeric initiator, as well as a source of ions. PEDOT:PSS is a semi-interpenetrating network (IPN) that has been used in recent studies to exhibit quick self-healing properties with the H2SO3 additive, which further improves its mechanical strength and self-healing performance. A moderate amount of PEDOT:PSS in the hydrogel (5 mL) was found to significantly improve the ionic conductivity compared to the pure hydrogel of alginate. Interestingly, the alginate/PEDOT:PSS composite hydrogel exhibited an excellent ability to self-heal and repair its original composition within 10 min of cutting. Furthermore, the graphite conductive substrate-based supercapacitor with the alginate/PEDOT:PSS hydrogel electrolyte provided a high specific capacitance of 356 F g-1 at 100 mV/s g-1. The results demonstrate that the A/P:P ratio with 5 mL PEDOT:PSS had a base sheet resistance of 0.9 Ω/square. This work provides a new strategy for designing flexible self-healing hydrogels for application in smart wearable electronics.