RESUMO
The water soluble matrix (WSM) of pearl powder [Hyriopsis cumingii Lea (Unionidae)] was extracted, and the insoluble residue was demineralized, size-fractionated, and named as MR14 (> 14 kDa), MR3-14 (3-14 kDa), and MR3 (< 3 kDa). The effects of WSM, MR14, MR3-14, and MR3 on primary mouse oral fibroblast proliferation, collagen accumulation, matrix metalloproteinase-2, -9 (MMP-2, -9) activities, and tissue inhibitor of metalloproteinase-1 (TIMP-1) production were tested by MTT assay, chloramine T method, gelatin zymography, and enzyme-linked immunosorbent assay (ELISA), respectively. The results showed that the WSM and MR14 could significantly (p < 0.05) promote fibroblast proliferation; all of the fractions could significantly promote collagen accumulation; MR14 significantly (p < 0.05) inhibited MMP-2 activity; and all of the fractions could significantly promote TIMP-1 production. This study has proved that the mechanism by which pearl powder promotes wound healing is partly due to its ability to stimulate fibroblast mitosis, collagen deposition, and TIMP-1 production, and the major active fraction may be MR14.
Assuntos
Antiulcerosos/farmacologia , Materia Medica/farmacologia , Medicina Tradicional Chinesa , Mucosa Bucal/efeitos dos fármacos , Unionidae/química , Cicatrização/efeitos dos fármacos , Animais , Antiulcerosos/química , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Fracionamento Químico , Colágeno/metabolismo , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Materia Medica/química , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Mucosa Bucal/citologia , Mucosa Bucal/metabolismo , Pós , Solubilidade , Fatores de Tempo , Inibidor Tecidual de Metaloproteinase-1/metabolismoRESUMO
<p><b>OBJECTIVE</b>To research molluscicidal effect activity, active components and stable passage of endophyte JJ18 from Pseudolarix amabilis and examine the possibility for practical application.</p><p><b>METHOD</b>Molluscicidal effect test was performed according to the immersion test method suggested by WHO.</p><p><b>RESULT</b>Immersion test with JJ18 broth showed that the active components were extracellular moiety of the broth and that 10% concentration solution could kill nearly 90% snail immersed after 72 h, the salified broth has favourable thermostabily and photostability and showed that JJ18 has stable passage and its active components concentrate in the extract of n-butanol.</p><p><b>CONCLUSION</b>The metabolite of endophyte JJ18 has activity for molluscicidal effect and potential for application.</p>
