Expression and role of FKBPL in lung adenocarcinoma.

Dysregulated expression of FK506-binding protein like (FKBPL) has been demonstrated to play crucial roles in tumour development. However, the role of FKBPL in lung adenocarcinoma (ADC) remains unclear. Using immunohistochemical staining, we showed that FKBPL expression was significantly lower in lung ADC than the normal tissues (P < 0.0001). Patients with well or moderately differentiated tumours have higher FKBPL expression compared with patients with poor differentiated tumours (P = 0.037). However, no significant associations were found between FKBPL expression and other clinicopathological variables (P > 0.05 for all). Cox univariate analysis showed that high FKBPL expression was correlated with prolonged overall survival (OS) (P = 0.010). Kaplan-Meier analysis further confirmed that the FKBPL-low group showed a significantly shorter OS than the FKBPL-high group (P = 0.0081). FKBPL expression was not shown as an independent prognostic factor for OS in the multivariate analysis (P = 0.063). Moreover, our study demonstrated that FKBPL could suppress the proliferation of lung ADC cells by delaying cell cycle G1/S phase transition. In addition, FKBPL resulted in increased apoptosis in lung ADC cells. Using the Human Apoptosis Array Kit, we observed that overexpression of FKBPL in lung ADC A549 cells significantly decreased the anti-apoptotic proteins, including heat shock protein 32 (HSP32), heat shock protein 27 (HSP27), and paraoxonase-2 (PON2). FKBPL depletion significantly attenuated the pro-apoptotic protein, phospho-p53 (S46), in lung ADC H1975 cells. These new findings provide an experimental basis for further theoretical investigation of lung ADC.

Pulmonary adenofibroma with central liquefaction necrosis: A case report.

Pulmonary adenofibroma (PAF) is a rare benign tumor. Computed tomography (CT) imaging of PAF show well-defined, homogeneous and solitary nodules. To the best of our knowledge, there is no report of PAF presenting with central liquefaction necrosis on CT images. The present study reports the case of a 70-year-old man who was hospitalized due to an inguinal hernia without respiratory symptoms. Chest CT scan revealed a tumor (~6.5x5.5x4.4 cm) in the lower lobe of the left lung, characterized by uneven density and unclear boundary with the pleura. Contrast-enhanced scan revealed that the lesion was slightly enhanced and liquefaction necrosis appeared in its center. Wedge resection was performed using video-assisted thoracic surgery. Histopathological and immunohistochemical examination confirmed the diagnosis of PAF.