UPLC-MS-Based Metabolomics Profiling and Chemometric Analysis for Hypericum sinaicum Boiss and the Endophytic Aspergillus foetidus in Comparison to Hypericum perforatum L.

One of the endangered plant species in Saint Catherine protectorate is Hypericum sinaicum Boiss which is endemic to Egypt, Jordan, and Saudi Arabia. The fungus-host relationship can assist in the investigation of bioactive compounds produced by H. sinaicum paving the way for economic and medicinal implications. Therefore, a comprehensive metabolic approach via MS and chemical analysis was used to track and compare metabolites from H. sinaicum and Aspergillus foetidus var. pallidus, the endophytic fungus, with Hypericum perforatum. Metabolomics analysis revealed the presence of 25 metabolites distributed among samples and the discovery of new chemotaxonomic compounds, i. e., phloroglucinols and xanthones, allowing the discrimination between species. A. foetidus extract is considered a reliable source of furohyperforin and naphthodianthrone derivatives. In conclusion, using A. foetidus as an in vitro technique for producing potential phytoconstituents was cost effective, having easier optimization conditions and faster growth with fewer contamination rates than other in vitro methods.

Red and white cabbages: An updated comparative review of bioactives, extraction methods, processing practices, and health benefits.

Red and white cabbages (Brassica oleracea var. capitata f. alba and rubra, respectively) are two of the most commercially valued vegetables in crucifers, well-recognized for their unique sensory and nutritive attributes in addition to a myriad of health-promoting benefits. The current review addressed the differential qualitative/quantitative phytochemical make-ups for the first time for better utilization as nutraceuticals and to identify potential uses based on the chemical makeup of both cultivars (cvs.). In addition, extraction methods are compared highlighting their advantages and/or limitations with regards to improving yield and stability of cabbage bioactives, especially glucosinolates. Besides, the review recapitulated detailed action mechanism and safety of cabbage bioactives, as well as processing technologies to further improve their effects are posed as future perspectives. White and red cabbage cvs. revealed different GLSs profile which affected by food processing, including enzymatic hydrolysis, thermal breakdown, and leaching. In addition, the red cultivar provides high quality pigment for industrial applications. Moreover, non-conventional modern extraction techniques showed promising techniques for the recovery of their bioactive constituents compared to solvent extraction. All these findings pose white and red cabbages as potential candidates for inclusion in nutraceuticals and/or to be commercialized as functional foods prepared in different culinary forms.

UV Fingerprinting Approaches for Quality Control Analyses of Food and Functional Food Coupled to Chemometrics: A Comprehensive Analysis of Novel Trends and Applications.

(1) Background: Ultraviolet-visible (UV-Vis) spectroscopy is a common analytical tool to detect chromophore in compounds by monitoring absorbance spectral wavelengths. Further, it could provide spectral information about complex conjugated systems in mixtures aided by chemometric tools to visualize large UV-Vis datasets as typical in food samples. This review provides novel insight on UV-Vis applications in the last 20 years, as an advanced analytical tool in the quality control of food and dietary supplements, as well as several other applications, including chemotaxonomy, authentication, fingerprinting, and stability studies. (2) Conclusions: A critical assessment of the value of UV application and its novel trends in the quality control (QC) of nutraceuticals reveals the advantages and limitations, focusing on areas where future advancements are in need. Although simple, UV and its novel trends present potential analytical tools with an acceptable error for QC applications from a non-targeted perspective compared to other expensive spectral tools.

Facile solvothermal synthesis of a MIL-47(V) metal-organic framework for a high-performance Epoxy/MOF coating with improved anticorrosion properties.

The vanadium-based metal-organic framework MIL-47 distinguishes itself among other MOFs for its distinctive structure and unique properties (e.g., flexible structure, high thermal stability, and high surface area). The synthesis of MIL-47 has been reported from various metal precursors, including vanadium(iii) chloride (VCl3) as a rich source of metal ions. Attempts have been made to include other starting materials, a step forward towards large-scale production. Synthesis from various solid materials is encouraged, seeking an economic and greener approach. In this study, vanadium pentoxide (V2O5), a readily abundant low-cost and thermodynamically stable metal source, was used to synthesize the MIL-47(V) framework via a facile solvothermal route. This precursor provides a controllable rate of metal ion production depending on the applied reaction conditions. In our method, the synthesis took place at a low temperature and reaction time (180 °C for 20 h, instead of 220 °C for 72 h), yielding MIL-47 microrods. Moreover, among its unique properties, the metal centers of MIL-47 oxidize under the influence of thermal or chemical treatments, preserving the framework structure. This unusual character is not commonly witnessed in comparable MOF structures. This property can be leveraged in anti-corrosion applications, whereby a redox reaction would sacrifice the framework components, protecting the metal in contact. However, the chemical stability of MIL-47 is doubted against a corrosive medium. Thus, an epoxy coating with 10 wt% MOF loading was incorporated in our investigation to extend the aluminum alloy (AA2024) surface protection for prolonged exposure duration. The uniformity of distribution of the prepared MOF within the epoxy matrix was confirmed using SEM/EDX. Electrochemical impedance spectroscopy (EIS) was used to evaluate the corrosion performance of the coated samples. The results showed that the inclusion of V-MOF offers extended corrosion prevention, over 60 days, for the AA2024 alloy against artificial seawater. The neat epoxy coating could not prevent the corrosion of AA2024 over two weeks of immersion, whereby pitting corrosion was clearly observed. The V-MOF could induce a series of redox reactions leading to the precipitation of vanadium on the cathodic sites of metal surfaces.

Antiinflammatory and antioxidant activity of Hypericum sinaicum Boiss. growing widely in Egypt.

Methanol extract of the flowering aerial parts of Hypericum sinaicum Boiss. (ME) growing in Saint Catherine Protectorate (SKP), Egypt was analysed for its phenolic compounds profiling using HPLC and colorimetric methods. The total phenol content of ME was 158.60 ± 0.74 (µg GAE/mg D.E.), while the total flavonoid content was 70.91 ± 0.01 (µg QE/mg D.E.). HPLC analysis revealed that the highest flavonoid was naringenin (50.31 mg/g), while the highest phenolic acid was syringic acid (0.37 mg/g). The scavenging activity of ME was evaluated using DPPH assay with SC50 22.9 µg/ml and ABTS with SC50 13.10 µg/ml. ME produced dose - dependent and significant inhibition of edema at 4 hour of dose 200 mg/kg (78.55%) and 100 mg/kg (72.89%) to that of standard drug Indomethacin (86.94%). The current study interprets the anti-inflammatory and antioxidant potency of H. sinaicum.

MUFA in metabolic syndrome and associated risk factors: is MUFA the opposite side of the PUFA coin?

Omega-9 fatty acids represent some of the main mono-unsaturated fatty acids (MUFA) found in plant and animal sources. They can be synthesized endogenously in the human body, but they do not fully provide all the body's requirements. Consequently, they are considered as partially essential fatty acids. MUFA represent a healthier alternative to saturated animal fats and have several health benefits, including the prevention of metabolic syndrome (MetS) and its complications. This review concentrates on the major MUFA pharmacological activities in the context of MetS management, including alleviating cardiovascular disease (CVD) and dyslipidemia, central obesity, non-alcoholic fatty liver disease (NAFLD), and type 2 diabetes mellitus (T2DM). The beneficial effects of MUFA for CVD were found to be consistent with those of polyunsaturated fatty acids (PUFA) for the alleviation of systolic and diastolic blood pressure and high low density lipoprotein cholesterol (LDLc) and triacylglcerol (TAG) levels, albeit MUFA had a more favorable effect on decreasing night systolic blood pressure (SBP). To reduce the obesity profile, the use of MUFA was found to induce a higher oxidation rate with a higher energy expenditure, compared with PUFA. For NAFLD, PUFA was found to be a better potential drug candidate for the improvement of liver steatosis in children than MUFA. Any advantageous outcomes from using MUFA for diabetes and insulin resistance (IR) compared to using PUFA were found to be either non-significant or resulted from a small number of meta-analyses. Such an increase in the number of studies of the mechanisms of action require more clinical and epidemiological studies to confirm the beneficial outcomes, especially over a long-term treatment period.