RESUMO
OBJECTIVES: We evaluated the significance of urinary retinoic acid receptor-ß2 (RAR-ß2) gene promoter methylation and hyaluronidase activity in comparison with voided urine cytology (VUC) in diagnosis of bladder cancer. DESIGN AND METHODS: This study included 100 patients diagnosed with bladder cancer, 65 patients with benign urological disorders and 51 healthy volunteers. Urine supernatant was used for determining hyaluronidase activity by zymography while urine sediment was used for cytology and detection of methylated RAR-ß2 gene promoter by methylation specific nested PCR. RESULTS: The sensitivity and specificity were 53% and 90.5% for VUC, 65% and 89.7% for percent methylation fraction of RAR-ß2 gene promoter, and 89% and 90.5% for hyaluronidase activity; combination of the three parameters increased sensitivity to 95%. A significant association was observed between investigated markers and advanced grade tumor. CONCLUSIONS: Combined use of RAR-ß2 gene promoter methylation, hyaluronidase activity and VUC is promising non-invasive tool for bladder cancer detection.
Assuntos
Antígenos de Neoplasias/urina , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células de Transição/diagnóstico , Metilação de DNA , Histona Acetiltransferases/urina , Hialuronoglucosaminidase/urina , Receptores do Ácido Retinoico/genética , Neoplasias da Bexiga Urinária/diagnóstico , Adulto , Idoso , Biomarcadores Tumorais/urina , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/parasitologia , Carcinoma de Células Escamosas/urina , Carcinoma de Células de Transição/genética , Carcinoma de Células de Transição/parasitologia , Carcinoma de Células de Transição/urina , Estudos de Casos e Controles , DNA/isolamento & purificação , DNA/urina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Regiões Promotoras Genéticas , Curva ROC , Receptores do Ácido Retinoico/metabolismo , Esquistossomose/complicações , Esquistossomose/imunologia , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/parasitologia , Neoplasias da Bexiga Urinária/urina , Urina/citologiaRESUMO
OBJECTIVES: Acute lymphoblastic leukemia (ALL) is an aggressive cancer especially in adults as only 20-40% are cured with current treatment regimens. DESIGN AND METHODS: We measured survivin and tumor necrosis factor-alpha (TNF-α) in serum of 30 ALL patients before and after induction therapy and compared to 30 age and sex matched normal adults. RESULTS: Survivin at cutoff value 15.18 pg/mL was detected in all ALL patients before therapy but in only 83.33% after therapy and not detected in the control group; P<0.001. However TNF-α at cutoff value 60.05 pg/mL was detected in 90% ALL patients before therapy and 86.6% after therapy that was significantly higher than the control group (20%); P<0.001. Survivin showed a significant positive correlation with TNF-α (P<0.05), bone marrow blast cells (P<0.01), peripheral blast cells (P<0.05) and Philadelphia chromosome (P<0.01). CONCLUSIONS: Survivin may have an important role in the development of acute leukemia and it could serve as a significant prognostic marker.
Assuntos
Proteínas Inibidoras de Apoptose/sangue , Leucemia-Linfoma Linfoblástico de Células Precursoras/sangue , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Fator de Necrose Tumoral alfa/sangue , Adulto , Biomarcadores Tumorais , Feminino , Regulação Leucêmica da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Cromossomo Filadélfia , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Prognóstico , Curva ROC , Fatores de Risco , Sensibilidade e Especificidade , Survivina , Resultado do TratamentoRESUMO
OBJECTIVES: Oxidative stress plays a role in the pathogenesis in patients with HCV infection. The objective of this study was to evaluate oxidant and antioxidant biomarkers in patients with HCV. DESIGN AND METHODS: Serum malonaldehyde (MDA) and nitric oxide (NO) levels and the activities of myeloperoxidase (MPO), arylesterase (AE) and paraoxonase-1 (PON1) were determined in 23 chronic and 21 cirrhotic patients with HCV and 21 healthy subjects. RESULTS: Cirrhotic patients with HCV had higher serum NO level and MPO activity while lower AE and PON1 activities than the chronic. Significant inverse correlation was observed between MDA and PON1 activity in patients with HCV. The most significant HCV biomarker was MDA, AE, NO and PON1. The best combined ones for sensitivity, specificity were MDA+albumin, PON1+AST, and PON1+albumin. CONCLUSIONS: The use of the MDA, MPO, AE, NO and PON1 as biomarkers might be useful tools, helping in the monitoring of patients with HCV.