RESUMO
Three methods of analysis are conducted that need computational procedures by the Matlab® software. The first is the univariate mean centering method which eliminates the interfering signal of the one component at a selected wave length leaving the amplitude measured to represent the component of interest only. The other two multivariate methods named PLS and PCR depend on a large number of variables that lead to extraction of the maximum amount of information required to determine the component of interest in the presence of the other. Good accurate and precise results are obtained from the three methods for determining clotrimazole in the linearity range 1-12⯵g/mL and 75-550⯵g/mL with dexamethasone acetate 2-20⯵g/mL in synthetic mixtures and pharmaceutical formulation using two different spectral regions 205-240â¯nm and 233-278â¯nm. The results obtained are compared statistically to each other and to the official methods.