RESUMO
Cellular metabolic reprogramming is the main characteristic of cancer cells and identification of targets using this metabolic pattern is extremely important to treat cancers, such as osteosarcoma (OS). In this study, SLIT2 and ROBO1 were upregulated in OS, and higher expression of ROBO1 was associated with worse overall survival rate. Furthermore, in vitro and in vivo experiments demonstrated that the SLIT2/ROBO1 axis promotes proliferation, inhibits apoptosis, and contributes to the Warburg effect in OS cells. Mechanistically, the SLIT2/ROBO1 axis exerted cancer-promoting effects on OS via activation of the SRC/ERK/c-MYC/PFKFB2 pathway. Taken together, the findings reveal a previously unappreciated function of SLIT2/ROBO1 signaling in OS, which is intertwined with metabolic alterations that promote cancer progression. Targeting the SLIT2/ROBO1 axis may be a potential therapeutic approach for patients with OS.
Assuntos
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Osteossarcoma/metabolismo , Fosfofrutoquinase-2/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Receptores Imunológicos/metabolismo , Quinases da Família src/metabolismo , Animais , Glicólise , Xenoenxertos , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Proteínas do Tecido Nervoso/genética , Osteossarcoma/genética , Fosforilação Oxidativa , Fosfofrutoquinase-2/genética , Proteínas Proto-Oncogênicas c-myc/genética , Receptores Imunológicos/genética , Transdução de Sinais , Quinases da Família src/genética , Proteínas RoundaboutRESUMO
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.
Assuntos
Glândulas Mamárias Animais/metabolismo , MicroRNAs/metabolismo , Animais , Feminino , Perfilação da Expressão Gênica , Lactação , Glândulas Mamárias Animais/patologia , Anotação de Sequência Molecular , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de RNA , SuínosRESUMO
Aqueous rechargeable sodium-ion batteries have the potential to meet growing demand for grid-scale electric energy storage because of the widespread availability and low cost of sodium resources. In this study, we synthesized a Na-rich copper hexacyanoferrate(II) Na2 CuFe(CN)6 as a high potential cathode and used NaTi2 (PO4 )3 as a Na-deficient anode to assemble an aqueous sodium ion battery. This battery works very well with a high average discharge voltage of 1.4 V, a specific energy of 48 Wh kg(-1) , and an excellent high-rate cycle stability with approximately 90 % capacity retention over 1000 cycles, achieving a new record in the electrochemical performance of aqueous Na-ion batteries. Moreover, all the anode, cathode, and electrolyte materials are low cost and naturally abundant and are affordable for widespread applications.
