1.
Bull Exp Biol Med
; 143(6): 723-6, 2007 Jun.
Artigo
em Inglês, Russo
| MEDLINE
| ID: mdl-18239811
RESUMO
We developed a technology of labeling bone marrow precursor cells with the Lin-c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies.