RESUMO
The aim of this study was to systematically evaluate the correlation between the rs231775 locus polymorphism in the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) gene and genetic susceptibility to Graves' disease (GD) in children. Some studies found that the CTLA-4 gene polymorphism was associated with GD in children. The data up to February 2022 were retrieved from the databases. Stata 15.0 software was used for meta-analysis. A total of seven studies were included in our research. The results of the meta-analysis showed that the rs231775 locus polymorphism in the CTLA-4 gene in general and Asian populations was correlated with children's susceptibility to GD (A vs G: OR = 0.75, 95% CI (0.660-0.86); GG vs AA: OR = 1.34, 95% CI (1.04-1.73); AG vs AA: OR = 1.32, 95% CI (1.02-1.10); AG + GG vs AA: OR = 3.81, 95% CI (2.17-6.70); GG vs AA + AG: OR = 1.23, 95% CI (1.05-1.45)). In summary, the rs231775 locus polymorphism in the CLTA-4 gene may be a risk factor for GD in Asian children. The G allele may be a susceptibility factor, while the allele A may be a protective factor against GD in Asian children. In the future, more large-scale studies may be needed to verify our results.
RESUMO
OBJECTIVE: The present study aimed to elucidate the molecular network mechanism of the Rujiling capsule in the treatment of hyperplasia of mammary glands through network pharmacology and molecular docking. MATERIALS AND METHODS: TCMSP and TCMID databases were screened for the active components and their action targets of the Rujiling capsule, whereas the disease targets of hyperplasia of mammary glands were searched in GeneCard and DisGeNET databases. Venny software was employed to identify the common targets of drugs and diseases. Cytoscape software was used to construct the network pharmacological diagram of "drug-active components-target" and the intersection targets were subjected to protein-protein interaction analysis by STRING platform and Cytoscape software. The DAVID database was exploited for gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis of the intersection target. After that, the key target genes with a degree value greater than the median were verified with the active components in molecular docking. RESULTS: A total of 691 drug targets, 251 disease targets, and 108 intersection targets were obtained after retrieval and screening. Among the 686 items enriched by GO included 522 biological processes, 110 molecular functions, and 54 cellular components. At the same time, 114 signal pathways were enriched by KEGG. The results of molecular docking revealed that the docking energies of main active components and some core targets were all <-5 kcal/mol. CONCLUSION: Henceforth, highlighted the role of the Rujiling capsule in the treatment of hyperplasia of mammary glands through multiple components, multiple targets, and multiple signal pathways.
